The invention discloses a tissue culture method of graptopetabum paraguayense, relates to a cuttage method of a plant, and belongs to the field of plant cultivation methods. The method is characterized by comprising the following steps: scissoring simple leaf, branch stem or telome, drying pruning wound, smearing 1000ppm of naphthylacetic acid on the pruning wound; transplanting on a dedicated sand bed. The manufacturing method is as follows: washing and screening normal sand to leave 100-mesh of sand, mixing 80% of sand, 10% of soil and 10% of manure, covering a plastic film, tightly closing the greenhouse for 15-20 days, preparing the sand for later use. The cuttage method has the beneficial effects that the root of the graptopetabum paraguayense in the cuttage method disclosed by the invention has no rot phenomenon, and the survival rate is improved to 95%; a plant growth regulator and micronutrient element relate to the root development are used as main agents, the root differentiation time can be shortened, the problem of the graptopetabum paraguayense cuttage technology in application is solved.