38 results about "Murine embryo" patented technology

The invention belongs to the technical field of medicines, and in particular discloses application of fructose 1,6-diphosphate (FDP) in preparation of a fetus protection medicine for spontaneous abortion. In vitro and in vivo animal experiments show that the concentration of the FDP in peripheral blood and decidual tissues of patients with recurrent spontaneous abortion is decreased compared withnormal pregnancy. Compared with control pregnant mice, the model plasma and uterus FDP levels of pregnant mice with spontaneous abortion are reduced, the uterus macrophage M2 phenotype molecule expression of the pregnant mice with spontaneous abortion is decreased, the proportions of helper cells T(Th)2 and regulatory T cells are reduced, and the phenomena of poor endometrium decidualization and decreased embryonic trophoblast infiltration degree occur in an accompanied way. The results indicate that the supplement of the FDP can significantly improve the uterine immune tolerance pattern, decidualization and embryonic trophoblast infiltration in the pregnant mice with spontaneous abortion, and obviously reduces embryonic loss in the pregnant mice. Therefore, the FDP is expected to be an early-warning and diagnostic indicator for the patients with spontaneous abortion, and can be used for preparing the fetus protection medicine for the spontaneous abortion.

The invention provides a method for cultivating human induced pluripotent stem cells by using human urine cells as a feeder layer. Human pluripotent stem cells (hiPS) reprogrammed from adult male foreskin fibroblasts were cultured with subcultured human urine cells from the third to twelfth passages cultured after cryopreservation and thawing as trophoblast cells. The human urine cells used in the present invention replace mouse embryonic fibroblasts or mouse embryonic fibroblast cell lines used in traditional methods to cultivate human induced pluripotent stem cells, which reduces the contamination of heterologous cells in the culture system, and is more effective than mesenchymal Mesenchymal stem cells are easier to obtain. And it was proved that this culture system can expand human induced pluripotent stem cells in vitro, and maintain the biological characteristics and pluripotent stemness of hiPS cells for a long time, which provides the possibility for hiPS to enter clinical application.

The invention discloses a mouse embryo transplant glue and a preparation method thereof. The mouse embryo transplant glue is prepared by adding pregnant horse serum, chorionic gonadotropin, amniotic membrane substances, mouse plasma, and streptomycin to a pH of 7.0 ~7.2, prepared in HEPES buffer solution with a concentration of 20mmol / L; the addition amount of each substance in each mL of HEPES buffer solution is as follows: 0.01~0.05μg of pregnant horse serum, 100~375 units of chorionic gonadotropin, streptomyces 50-70 μg of prime, 20 μL of amniotic substance, and 8-10 μL of mouse plasma. The mouse embryo transplantation glue provided by the invention can not only adhere the embryo to the uterus, improve the implantation rate of the embryo, but also provide nutrition for the growth of the embryo after implantation, and greatly improve the survival rate of the mouse embryo.

A tool for mouse embryo transplantation preservation and its manufacturing method, comprising a centrifuge tube, a syringe, a rubber piston and a Pasteur tube, the rubber piston is inserted into the syringe jacket from the tail, and a part of the volume of the cone head of the syringe jacket is appropriately cut off to make the cone The width of the mouth is equal to the width of the outer diameter of the Pasteur tube. Properly cut off the two wings of the syringe jacket so that the length of the two wings is equal to or slightly greater than the diameter of the centrifuge tube. Insert the syringe jacket into the centrifuge tube and fix the jacket so that the jacket cannot be left or right in the centrifuge tube. Moving up and down, the Pasteur tube is fixed to the cone of the jacket. By adopting the invention, the transplanted embryos can be effectively preserved in the external environment, shortening the low temperature and low humidity time of the embryos in vitro, and facilitating the implantation and later development of the embryos.