38 results about "Murine embryo" patented technology

The invention belongs to the technical field of medicines, and in particular discloses application of fructose 1,6-diphosphate (FDP) in preparation of a fetus protection medicine for spontaneous abortion. In vitro and in vivo animal experiments show that the concentration of the FDP in peripheral blood and decidual tissues of patients with recurrent spontaneous abortion is decreased compared withnormal pregnancy. Compared with control pregnant mice, the model plasma and uterus FDP levels of pregnant mice with spontaneous abortion are reduced, the uterus macrophage M2 phenotype molecule expression of the pregnant mice with spontaneous abortion is decreased, the proportions of helper cells T(Th)2 and regulatory T cells are reduced, and the phenomena of poor endometrium decidualization and decreased embryonic trophoblast infiltration degree occur in an accompanied way. The results indicate that the supplement of the FDP can significantly improve the uterine immune tolerance pattern, decidualization and embryonic trophoblast infiltration in the pregnant mice with spontaneous abortion, and obviously reduces embryonic loss in the pregnant mice. Therefore, the FDP is expected to be an early-warning and diagnostic indicator for the patients with spontaneous abortion, and can be used for preparing the fetus protection medicine for the spontaneous abortion.

The invention discloses a method for rapidly and directly directionally inducing the differentiation of mouse embryonic stem cells into neuroepithelial cells, by adding an induction medium containing dorsomorphin and noggin, SB431542 and CHIR99021 to the adherent cultured mouse embryonic stem cell pellet clones to induce mouse Embryonic stem cells form rosette-shaped neurospheres; then add the second-stage induction medium containing bFGF to form suspended neurospheres; finally digest the neurospheres into single cells, and then use the third-stage induction medium to form adherent cultures Neuroepithelial cells with longer self-proliferation and renewal. The invention abandons the traditional EB forming method, shortens the differentiation time, and the differentiation efficiency reaches more than 95%. The invention can quickly induce NE cells to replace NSC cells to treat central system damage, and has high clinical applicability.

The invention belongs to the field of artificial intelligence, and specifically relates to a mouse embryo organ recognition and scoring method and system. The method includes the following steps: collecting original images of mouse embryos in different periods and manually labeling them; Input the Mask-RCNN network for training to obtain the organ recognition model; intercept the organs in the marked image from the original image, and use it and the corresponding score as the training set data, and train different convolutional neural networks respectively. Obtain an image scoring model that can score the development of each organ separately; input the original image of the mouse embryo to be recognized into the organ recognition model, and output all organs in the image; intercept all organs from the original image, and input them into the image scoring model Model, get the development score of each organ; get the total score. The invention can quickly and accurately identify the organs in the mouse embryo, and judge the current development stage of each organ.

The invention provides a method for cultivating human induced pluripotent stem cells by using human urine cells as a feeder layer. Human pluripotent stem cells (hiPS) reprogrammed from adult male foreskin fibroblasts were cultured with subcultured human urine cells from the third to twelfth passages cultured after cryopreservation and thawing as trophoblast cells. The human urine cells used in the present invention replace mouse embryonic fibroblasts or mouse embryonic fibroblast cell lines used in traditional methods to cultivate human induced pluripotent stem cells, which reduces the contamination of heterologous cells in the culture system, and is more effective than mesenchymal Mesenchymal stem cells are easier to obtain. And it was proved that this culture system can expand human induced pluripotent stem cells in vitro, and maintain the biological characteristics and pluripotent stemness of hiPS cells for a long time, which provides the possibility for hiPS to enter clinical application.

The invention discloses a mouse embryo transplant glue and a preparation method thereof. The mouse embryo transplant glue is prepared by adding pregnant horse serum, chorionic gonadotropin, amniotic membrane substances, mouse plasma, and streptomycin to a pH of 7.0 ~7.2, prepared in HEPES buffer solution with a concentration of 20mmol / L; the addition amount of each substance in each mL of HEPES buffer solution is as follows: 0.01~0.05μg of pregnant horse serum, 100~375 units of chorionic gonadotropin, streptomyces 50-70 μg of prime, 20 μL of amniotic substance, and 8-10 μL of mouse plasma. The mouse embryo transplantation glue provided by the invention can not only adhere the embryo to the uterus, improve the implantation rate of the embryo, but also provide nutrition for the growth of the embryo after implantation, and greatly improve the survival rate of the mouse embryo.

A tool for mouse embryo transplantation preservation and its manufacturing method, comprising a centrifuge tube, a syringe, a rubber piston and a Pasteur tube, the rubber piston is inserted into the syringe jacket from the tail, and a part of the volume of the cone head of the syringe jacket is appropriately cut off to make the cone The width of the mouth is equal to the width of the outer diameter of the Pasteur tube. Properly cut off the two wings of the syringe jacket so that the length of the two wings is equal to or slightly greater than the diameter of the centrifuge tube. Insert the syringe jacket into the centrifuge tube and fix the jacket so that the jacket cannot be left or right in the centrifuge tube. Moving up and down, the Pasteur tube is fixed to the cone of the jacket. By adopting the invention, the transplanted embryos can be effectively preserved in the external environment, shortening the low temperature and low humidity time of the embryos in vitro, and facilitating the implantation and later development of the embryos.

The invention discloses an albumin / keratin double expression mice embryonic stem cell system and an establishing method thereof. The mice embryonic stem cell system of the invention is established by sub-culturing the albumin / keratin double expression mice embryonic stem cell. The mice embryonic stem cell is integrated with a red fluorescence / green fluorescence protein double reporter gene, wherein the reporter gene is regulated by tissue specificity promoter albumin ALB or keratin CK19. Compared with a method of inputting genes, the method of controlling the reporter gene by using the transfection tissue specificity promoter of the invention is more simple, rapid and useful. The uniform originator cells in the developmental stage can be precisely chosen by using the albumin / keratin double expression mice embryonic stem cell of the invention, which can bring a great beneficial effect to the following research on the originator cell differentiation, the cell transplanting and cure.

The invention relates to an experimental method for inducing directional differentiation of neural stem cells into neuron cells by Gd:Fe3O4@RA nanoparticles. The experimental method includes the following steps of putting a cover slip pre-treated with polylysine into a culture plate, and inoculating culture holes with primary and passaged neural stem cells derived from rat embryonic brain hippocampus at 1 is multiplied by 10<7> L<-1>, removing basic fibroblast growth factors from a complete culture solution of the neural stem cells, adding 1.0 ml of Gd:Fe3O4@RA complex nano ions with the concentration of 1 mg / ml and fetal bovine serum with the volume fraction of 5% to promote neural stem cell differentiation, performing culture for 6 days, continuing culture for 6 days in a neural stem cell culture medium containing the fetal bovine serum with the volume fraction of 5%, and taking out the cover slip after the cell morphology is mature.