37 results about "Murine embryo" patented technology

The invention belongs to the field of artificial intelligence, and particularly relates to a mouse embryo organ recognition and scoring method and a system. The method comprises the following steps: collecting original images of mouse embryos in different periods, and carrying out the manual marking; inputting the mouse embryo original image and the annotation file into a Mask-RCNN network for training to obtain an organ recognition model; intercepting organs in the marked image from the original image, taking the organs and corresponding scores as training set data, and training different convolutional neural networks to obtain an image scoring model capable of scoring development of each organ; inputting a to-be-recognized mouse embryo original image into the organ recognition model, and outputting all organs in the image; intercepting all organs from the original image, and respectively inputting the organs into an image scoring model to obtain a development score of each organ; and obtaining a total score. According to the method, the organs in the mouse embryo can be quickly and accurately recognized, and the current development stage of each organ can be judged.

The invention provides a novel body cell reprogramming inducing method, a kit and an application and relates to an application of an ectodermal differentiation and development correlation factor and/or mesendodermal differentiation and development correlation factor as an inducing factor to generation of an induced pluripotent stem cell. According to the invention, the inducing factor is provided for a differentiated stem cell to induce to generate the induced pluripotent stem cell (iPS cell), and the inducing factor comprises the mesendodermal differentiation and development correlation factor, Sox2, Klf4 and any one c-Myc or the ectodermal differentiation and development correlation factor, Oct4, Klf4 and any one c-Myc or the mesendodermal differentiation and development correlation factor, the ectodermal differentiation and development correlation factor, Klf4 and any one c-Myc. The iPS cell obtained by using the novel body cell reprogramming inducing method has characteristics similar to those of a rat embryonic stem cell, namely has self-renewal maintaining capacity and differentiation omnipotency.

The invention relates to the technical field of medicines, in particular to application of rapamycin in preparation of a miscarriage prevention medicine for spontaneous abortion. Compared with normalpregnancy, macrophages residing in decidua tissue of a patient suffering from recurrent spontaneous abortion are reduced, and expression of adhesion molecules is low; and the autophagy level of the decidual macrophages of the abortion patient is low. Experimental results show that the rapamycin can significantly promote adhesion of the macrophages to decidua matrix cells. Normal pregnant rats andabortion pregnant rat models are constructed, and in-vivo experiments prove that: the rapamycin is applied to reduce dwelling of decidual macrophages caused by autophagy disorders, maintains the steady state of decidua immune components and significantly reduces the embryo absorption rate of the pregnant rats, thereby improving the outcome of bad pregnancy. Therefore, the rapamycin is expected tobe used as the miscarriage prevention medicine for spontaneous abortion, a new treatment scheme is provided for patients suffering from repeated spontaneous abortion, the psychological burden of the patients is relieved, and the rapamycin can be well applied clinically.

The invention discloses a non-alcoholic fatty liver mouse model and a construction method thereof. The construction method includes the specific construction step that 1, mouse embryonic stem cells are transfected, and a fourth exon segment and a fifth exon segment containing ghr genes are amplified through Pfu Turbo DNA polymerase and cloned into a pCR Blunt II-TOPO vector. When a mouse modeled through the method is 8 weeks old, the liver volume is remarkably increased, H&E staining and oil red staining results show fatty liver lesion, liver cell fat synthesis is remarkably increased, lipolysis is remarkably reduced, and the mouse model built through the method has the advantages of being stable in heredity, similar to human conditions in clinical symptoms, short in modeling time, high in efficiency and low in death rate. The model is capable of being formed without diet induction, and relatively wide in application and popularization prospect, and is an ideal non-alcoholic fattyliver animal model for drug screening and pharmacological research.

The invention discloses a method for promoting differentiation of rat radial glial cells into neurons, comprising the following steps of: acquiring a fetal rat cerebral cortex cell suspension, and preparing RGCs single cells; and inoculating the RGCs single cells into a culture plate, culturing overnight by using an RGCs culture solution, and continuously culturing by using an RGCs induced differentiation solution. The method for preparing the RGCs single cells comprises the following steps of: resuspending cells by using an RGCs culture solution to form macroscopic suspended cell spheres, replacing the RGCs culture solution, and continuously culturing for 5-7 days to obtain the cell spheres; and treating the cell spheres with Accutase enzyme, digesting the cell spheres into single cells,continuing suspension culture, and carrying out passage. According to the method, the differentiation ratio of the rat embryo cerebral cortex derived radial glial cells to neurons can be obviously improved and can reach about 3 times or even higher than the natural differentiation rate.

The present invention discloses a method for improving induction efficiency of neural stem cells and promoting differentiation of neurons. A programmed induction of SMSINS cells is as follows: using LDN193189 (Selleck S2618, 0.25 [mu]M) and SB431542 (Selleck S1067, 1 [mu]M) on the 1st and 2nd days to treat mouse embryonic fibroblasts (MEFs); using CHIR99021 (Selleck S2924, 3 [mu]M) DAPT (Selleck S2215, 5 [mu]M) and VPA (Sigma P4543, 0.5 mM) on the 3rd and 4th day; using CHIR99021 (Selleck S2924, 3 [mu]M) and DAPT (Selleck S2215, 5 [mu]M) on the 5th and 6th days; adding Shh (Peprotech 315225, 100 ng/ml) and Purmorphamine (selleck S3042, 1 [mu]M) on the 7th and 8th days to improve neural differentiation; and removing all small molecule compounds on the 9th and 10th days and conducting changeto a NSC complete culture medium. At the time, SMSINS cells can be trypsinized into single cells and can be inoculated into 24-well plates without coating PDL/L in the NSC complete culture medium; and the SMSINS cells are subjected to in vitro neuronal differentiation. The method is simple and convenient to operate, can improve induction of the neural stem cells and promote the differentiation ofthe neurons, and is beneficial to popularization and application.

The invention discloses a method for obtaining the submandibular gland of mouse embryos, and relates to the field of biomedicine. The method includes: freeing the uterus: after killing the pregnant mouse, freeing the uterus containing the embryo completely; freeing the embryo: opening the uterine wall, freeing the embryo completely; obtaining embryonic jaw tissue: obtaining the jaw tissue from the embryo under a dissecting microscope ;Obtain embryonic submandibular gland: place the mandibular tissue under a dissecting microscope, zoom in on the field of view clearly and then fix it. After separating the most superficial tissue, pinch off the connection between the submandibular gland, the tongue body and the duct, and then obtain the complete embryonic submandibular gland. This method improves the operation of obtaining the submandibular gland of mouse embryos. Compared with the traditional method, the operation steps are reduced, the operation difficulty is reduced, and the integrity of the extracted submandibular gland tissue is guaranteed to a greater extent, thus providing a better method. The model of submandibular gland cultured in vitro provides a reliable platform for the research of submandibular gland related diseases.

The invention relates to the technical field of medicine, and provides a method of screening and identifying small molecule with anti-aging properties. The method comprises cellular models, namely premature aging mouse embryonic fibroblasts and human HGPS mesenchymal stem cells and animal models, namely premature aging progeroid mice and Caenorhabditis elegans. The method successfully screened and identified Cannabidiol (CBD), which had senolytic effects. The invention also determined that the optimal concentration of CBD treatment is 10-20 μM.

The invention provides an application of a polymethyl methacrylate material in manufacturing a CUBIC transparent tissue microscopic imaging holder. The invention also provides a method for preparing the CUBIC transparent tissue microscopic imaging holder, which comprises the following steps: adding a polymethyl methacrylate material into a blow molding device, an injection device or an extrusion device for molding. The polymethyl methacrylate material can be reshaped into holding devices with various shapes and sizes after being heated, and is suitable for various biological tissue samples after CUBIC transparency, such as zebra fish, mouse embryo, brain and human tumor tissues. The holder prepared from the material disclosed by the invention can be fixedly transferred in a light sheet microscope sample bin, and a sample to be shot can be quickly and conveniently placed in the holder, so that the transparent sample can be imaged more completely and conveniently.

The invention belongs to the field of medicinal chemistry, and discloses a compound with a benzomorpholone-biphenyl skeleton as well as a preparation method and application thereof. The compound has a structural general formula (I) as shown in the specification. The preparation method of the compound is simple and reliable, and the total yield is more than 40%. Preliminary pharmacological experiments show that the compound has a certain relaxation effect on in-vitro artery blood vessels of rats, can inhibit proliferation of mouse embryo fibroblasts (NIH-3T3) and expression of type I collagen to a certain extent, and can be used for development of anti-hypertension drugs and anti-myocardial fibrosis drugs.

The invention relates to a mouse embryonic stem cell strain SXMUe002-A-1, which has a preservation number of CGMCC No: 23035, and is preserved in the China Center for Type Culture Collection on September 28, 2021. The invention also provides an application of the mouse embryonic stem cell strain SXMUe002-A-1 in preparation of a mouse hemophilia model. According to the obtained mutant cell strain SXMUe002-A-1, the growth and reproduction capability and the cellular morphology of the mutant strain are highly similar to those of a wild type, no base is deleted and inserted on an exon, only one base is mutated, and the mutant cell strain SXMUe002-A-1 is closer to clinical cases. The mutant cell strain SXMUe002-A-1 has the characteristics of mouse embryonic stem cells, can be subjected to mouse embryonic stem cell chimeric injection to obtain a hemophilia mouse model, and is of great significance in further hemophilia nonsense mutation mechanism research, hemophilia drug screening and gene therapy.

The invention discloses an application of andrographis paniculata flavonoid glycoside C in preparation of a medicine for preventing and treating Covid-19. A BEAS-2B human normal pulmonary epithelial cell model, an NIH-3T3 mouse embryonic cell model, a molecular docking technology and molecular dynamics simulation are adopted to prove that the andrographis paniculata flavonoid glycoside C has no obvious toxicity on the human normal pulmonary epithelial cell and the mouse embryonic cell; the compound has a strong affinity effect and an enzymatic activity inhibition effect on angiotensinase 2 on human normal pulmonary epithelial cells. The andrographis paniculata flavonoid glycoside C can be combined with human lung epithelial cells ACE2 to block the combination of the SARS-CoV-2 and the ACE2, so that the invasion path that the SARS-CoV-2 depends on the combination with the ACE2 to enter the cells is inhibited, and the effect of preventing and treating Covid-19 is achieved. By researching the cytotoxicity of the andrographis paniculata flavonoid glycoside C and the affinity and inhibitory activity of the andrographis paniculata flavonoid glycoside C to human lung epithelial cells ACE2, a new thought and scheme are provided for research, development and production of medicines for preventing and treating Covid-19.

The invention discloses a culture medium for epitaxy culture of a mouse embryo body, which contains a basic culture medium, fetal calf serum, human umbilical serum, sodium pyruvate, glutamine, penicillin-streptomycin, an N2 additive and a B27 additive, and can promote the continuous culture stage of an in-vitro mouse embryo to E8.0 on the basis of improving the culture success rate. A foundation is provided for development research of pre-implantation embryos and implantation stage embryos, the selection range of mouse embryo related experiment contents and experiment stages can be expanded by improving the culture success rate and prolonging the in-vitro culture time, and a basis can also be provided for in-vitro culture of other mammal embryos.

The invention relates to the technical field of culture of KM mouse embryos, in particular to an observation device for culture in vitro of KM mouse embryos. The observation device comprises an observation box, wherein an observation mirror is mounted at the top end of the observation box, first support rods are welded to four corners of the bottom end of the observation box, a fixing block is inclamping connection to the top ends of the first support rods, a first clamping groove cooperating with the corresponding first support rod is formed in the bottom end of the fixing block, a placing plate is welded to the top end of the fixing block, second clamping grooves are uniformly formed in the top end of the placing plate, a first clamping block is in clamping connection to the inner partof the corresponding second clamping groove, a sterilizing protection pad is bounded to the top ends of the first clamping blocks, a switch gate is in hinged connection to the peripheral wall body ofthe observation box, above the placing plate, and a plurality of observation windows are mounted at the middle of the switch gate. According to the observation device, the placing plate can be convenient to disassemble, so that the placing plate can be conveniently cleaned; and observation windows and the observation mirror are arranged, so that comprehensive observation of the KM mice can be realized.

The invention provides a method for directly reprogramming mouse embryo fibroblasts into melanocytes. The method comprises the following steps: preparing high-quality concentrated lentivirus for transfecting alternative transcription factors; directly reprogramming the mouse embryo fibroblasts into melanocytes; optimizing and screening transcription factors capable of being used for direct reprogramming of melanocytes; and identifying the function of inducing melanocytes. The invention relates to an optimized and efficient melanocyte direct reprogramming method and a simplified and optimized melanocyte direct reprogramming system, and the method mainly comprise two aspects: preparing high-quality concentrated viruses, optimizing reprogramming culture medium components, and improving functions of melanocytes obtained by direct reprogramming; screening the transcription factor with the most significant effect for direct reprogramming of melanocytes; carrying out functional identification on the directly reprogrammed melanocytes, and providing a new treatment strategy for patients suffering from depigmentation diseases such as vitiligo.