Method for quickly, directly and directionally inducing differentiation from mouse embryonic stem cells to neuroepithelial cells

A technology of neuroepithelial cells and embryonic stem cells, applied in animal cells, nervous system cells, vertebrate cells, etc.
CN104450618AActive Publication Date: 2015-03-25南通大学技术转移中心有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
南通大学技术转移中心有限公司
Publication Date
2015-03-25

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Abstract

The invention discloses a method for quickly, directly and directionally inducing differentiation from mouse embryonic stem cells to neuroepithelial cells. The method comprises the steps of adding an induction medium containing dorsomorphin, noggin, SB431542 and CHIR99021 to a bulk clone of the mouse embryonic stem cells subjected to adherent culture, to induce the mouse embryonic stem cells to form rosette neurospheres; then adding a second stage of induction medium containing bFGF and performing suspension culture to form suspended neurospheres; and finally, digesting the neurospheres into single cells, and then performing adherent culture with a third stage of induction medium to form the neuroepithelial cells with long-term self proliferation and update. By adopting the method, the traditional EB method is abandoned, the differentiation time is shortened, and the differentiation efficiency reaches over 95%. The method can be used for quickly inducing NE cells to substitute NSC cells to treat central system injury, and has very high clinical applicability.
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Description

technical field

[0001] The invention belongs to the field of biomedicine, in particular to a composition for rapidly and directly inducing the differentiation of mouse embryonic stem cells into neuroepithelial cells, especially a method for rapidly and directly inducing the differentiation of mouse embryonic stem cells into neuroepithelial cells using the composition, and A method for further directing neuroepithelial cells to differentiate into neuron cells or glial cells. Background technique

[0002] Neural stem cells (NSCs) are a kind of mother cells with division potential and self-renewal ability, which exist in adult brain tissue as a kind of stem cells. Mainly distributed in the ependyma, subventricular zone, striatum, hippocampal dentate gyrus and other regions, it is a kind of pluripotent cell that can self-renew and differentiate into a variety of neural cells in the adult nervous system. neurons, astrocytes, and oligodendrocytes. The discovery of neural stem ce...

Claims

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