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139 results about "Mesenchymal stem cell differentiation" patented technology
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The process in which a relatively unspecialized cell acquires specialized features of a mesenchymal stem cell. A mesenchymal stem cell is a cell that retains the ability to divide and proliferate throughout life to provide progenitor cells that can differentiate into specialized mesenchymal cells. [CL:0002452, GOC:BHF]
Method and device for activating stem cells
InactiveUS20100119492A1Enhance osteoblast phenotypeBioreactor/fermenter combinationsBiocideActive agentOsteoblast
Invention embodiments described herein include methods and devices for stimulating mesenchymal stem cells in a stem cell source to differentiate into osteoblasts capable of forming bone. Devices and methods described include exposing a stem cell source, such as bone marrow aspirate, adipose tissue and / or purified allogenic stem cells, to an active agent, in a manner effective to form activated stem cells.
Owner:DEPUY SYNTHES PROD INC
Methods and uses of connective tissue growth factor for differentiation of mesenchymal stem cells
The present invention relates to novel methods and compositions related to the administration of connective tissue growth factor, alone or in combination with other growth factors, compositions or compounds, to induce the differentiation of mesenchymal stem cells into chondrocytes or asteablasts.
Owner:UNIV OF SOUTH FLORIDA +1
Method for differentiating mesenchymal stem cells into neural cells
A method for differentiating mesenchymal stem cells of bone marrow into neural cells comprises culturing the mesenchymal stem cells in a medium containing epidermal growth factor(EGF), basic fibroblast growth factor(bFGF) and hepatocyte growth factor(HGF), and the neural cells produced thereby can be employed for the treatment of a neural disease.
Owner:PHARMICELL +1
Transplant material and method for fabricating the same
InactiveUS6989030B1Improved bone tissue repair speedGood biocompatibilityBone implantTissue cultureCell adhesionOsteoblast
A transplant material which is capable of imparting desired mechanical properties, elevating bone tissue repair speed and improving biocompatibility. This transplant material comprises an artificial and biologically inactive material, which is to be implanted in vivo as a substitute for bone tissue, and at least one type of cells selected from among osteoblasts and precursory osteoblasts which are adhered to the surface of the artificial material so that the artificial material is coated with the bone matrix produced by the cells. The artificial material involves not only a biologically inactive material but also a biologically inactive material coated with a biologically active substrate. This transplant material is produced by culturing mesenchymal stem cells collected from a living body to differentiate into at least one type of cells selected from among osteoblasts and precursory osteoblasts and then culturing the cells together with the artificial material to thereby adhere the differentiated cells on the surface of the artificial material and coat the surface of the artificial material with the bone matrix produced by the differentiated cells.
Owner:JAPAN TISSUE ENG
Inducing method for differentiating umbilical cord mesenchymal stem cells into nerve cells
ActiveCN101914493APromote differentiationNo insertion mutationsSkeletal/connective tissue cellsCell-Extracellular MatrixECM Protein
The invention relates to the field of biotechnology, in particular to an inducing method for differentiating umbilical cord mesenchymal stem cells into nerve cells, which comprises the following steps of: cultivating the separated umbilical cord mesenchymal stem cells in an MSC culture medium to spread a whole culture dish; cultivating the stably-subculturing mesenchymal stem cells in the culture dish which is spread with the extracellular matrix by means of the MSC culture medium to be 70% confluence; and leading the 70% confluent mesenchymal stem cells to sequentially pass through four inducing culture mediums to cultivate, wherein the four inducing culture mediums comprise the following factors of 5-azacytidine+Pam3CSK4, bFGF+Noggin, bFGF+RA+FGF8+Wnt3a, and Bmp4+Shh+RA+NGF+BDNF. The inducing method has the benefit effects of being free of chemical substances with cytotoxicity, transgenic technology and nerve cell co-cultivation, and being high in differentiating efficiency and shorter in time consumption.
Owner:SHANDONG QILU STEM CELL ENG
Differentiation of human mesenchymal stem cells to cardiac progenitor cells that promote cardiac repair
A method for treating a subject afflicted with a cardiac disorder, in vivo, comprises (i) inducing differentiation of a progenitor cell, in vitro, to a cardiogenic cell; and (ii) administering a therapeutically effective amount of the cardiogenic cell of step (i) to the subject, thereby treating the cardiac disorder in the subject. This invention further provides related articles of manufacture and methods.
Owner:THE TRUSTEES OF COLUMBIA UNIV IN THE CITY OF NEW YORK +1
Mesenchymal stem cell differentiation
Owner:NOVARTIS AG +2
Method of inducing differentiation of mesenchymal stem cells into neurons
InactiveUS20080176328A1Enhancing neuroprotectionInhibit cell growthNervous system cellsMammal material medical ingredientsPrimary motor neuronNeuron
The present invention relates to a method for inducing differentiation of bone marrow-derived mesenchymal stem cells into mature neurons by culturing them in an optimal medium supplemented with necessary composition. According to the pre-induction method of the invention and a method for inducing differentiation of mesenchymal stem cells into neurons by culturing them in neuronal induction media (NIM) containing butyl hydroxyanisole, forskolin and VPA, mesenchymal stem cells can be effectively differentiated into neurons or motor neurons, which thereby can be effectively used as a therapeutic agent for cell therapy for neurodegenerative diseases.
Owner:SEOUL NAT UNIV R&DB FOUND
Method for culturing mesenchymal stem cell and method for producing biological tissue prosthesis
InactiveUS20100028997A1Reduce concentrationReduce harmCulture processArtificial cell constructsProsthesisMesenchymal stem cell proliferation
The purpose is to proliferate a mesenchymal stem cell to a sufficient degree while reducing the amount of blood serum contained in a biological tissue progenitor cell to be grafted, and to efficiently differentiate the mesenchymal stem cell into the biological tissue progenitor cell. There is provided a method for culturing a mesenchymal stem cell, comprising: a first culture step of proliferating a mesenchymal stem cell in a medium containing blood serum; and a second culture step of differentiating the mesenchymal stem cell into a biological tissue progenitor cell in a medium containing blood serum at a lower concentration than that in the medium used in the first culture step.
Owner:OLYMPUS CORP
Method of preparing containing endothelium ancestor cell preparation utilizing umbilical or placenta and uses thereof
InactiveCN101199550AGood proliferative potentialConvenient sourceAntipyreticAnalgesicsProgenitorUmbilical cord
The invention discloses a method which adopts umbilical cord or placenta to prepare a preparation containing endothelial progenitor cells and an application of the preparation, mainly relating to a novel source of an endothelial progenitor cell preparation and a preparation method of the endothelial progenitor cell preparation. The stem cell preparation adopts the placenta or the umbilical cord as the source of the cells; mesenchymal stem cells are firstly separated and amplified; and then VEGF is added to induce the mesenchymal stem cells to be differentiated into the endothelial progenitor cells. The endothelial progenitor cell preparation prepared according to the invention contains a large number of endothelial progenitor cells. The preparation can be applied to clinical treatment and tissue engineering, etc. The raw material sources of the preparation are rich; the success rate of the separation and the yield are high; and the proliferation ability of the cells is strong.
Owner:INST OF HEMATOLOGY & BLOOD HOSPITAL CHINESE ACAD OF MEDICAL SCI
Bone repair composite for inducing mesenchymal stem cell differentiation and preparing method of bone repair composite
ActiveCN105999396AGood dispersionReduce agglomeration effectTissue regenerationProsthesisBiological propertyBioactive glass
The invention relates to a bone repair composite for inducing mesenchymal stem cell differentiation and a preparing method of the bone repair composite. The technical problem that existing materials are unsatisfactory in biological property is solved. The bone repair composite is formed by a graphene film and biologically-active glass, wherein the biologically-active glass is uniformly loaded on the surface of the graphene film and is in uniform spherical morphology. The bone repair composite and the preparing method can be applied to the field of bone repair.
Owner:PEKING UNIV SCHOOL OF STOMATOLOGY
Small RNA numerator for differentiation of mesenchyma stem cell into hematopoiesis cell and function target point thereof
The invention provides small RNA (shR337) capable of differentiating hematopoietic stem cells (MSC) from human nonhematopoietic stem cells (HSC) such as mesenchymal stem cells (MSC). The discovery of the small RNA also provides an action target capable of differentiating the hematopoietic stem cells from the human nonhematopoietic stem cells such as the bone marrow mesenchymal stem cells. The small RNA can restrict the protein translation of EIDI genes after being transfected to human bone marrow mesenchymal stem cells to transform the human bone marrow mesenchymal stem cells into hematopoietic stem cells with CD45<+>surface antigen positive, and the hematopoietic stem cells with CD45<+>surface antigen positive can be further differentiated into myeloid cells and lymphoid cells. Mice in vivo experiments show that the differentiation of the small RNA to the mesenchymal stem cells is directional and undivided.
Owner:INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
Mesenchymal stem cell differentiation
ActiveUS20120177644A1Ameliorating and preventing arthritisAmeliorating and preventing and joint injuryOrganic active ingredientsPeptide/protein ingredientsMedicineArthritis
The present invention provides for methods and compositions for treating or preventing arthritis and joint injury.
Owner:NOVARTIS AG +2
Building method of Pre-revascularizational large biological bone scaffold of composite cell
ActiveCN107412851AImplement the buildReduce harmAdditive manufacturing apparatusTissue regenerationOsteoblastDirect writing
The invention discloses a building method of a pre-revascularizational large biological bone scaffold of a composite cell which is used for the field of biological manufacturing. According to the building method of the pre-revascularizational large biological bone scaffold of the composite cell, composite biological 3D printing, electro-hydro dynamics direct writing, subtractive manufacturing and a cell assembly technology are adopted to build a functional pre-revascularizational network in the interior of the large biological bone scaffold to promote repairing of large bone defect. The key of the building method lies in the fact that sodium alginate which is a biological material and can be degraded in a body is adopted to serve as a sacrificial material, endothelial cells are loaded in the sodium alginate, and the sodium alginate is adhered to the surface of a prefabricated vessel network through migration of the endothelial cells. Meanwhile, the sodium alginate is degraded to form the prefabricated vessel network. In addition, human mesenchymal stem cells in a bone scaffold material are differentiated into osteoblasts, and synthesizes and secretes bone matrix under the mutual promotional effect of the endothelial cells, mineralization is conducted, so that the efficiency of bone tissue repair is improved, the functional problem of large bone tissue repair is solved, and the building method has important significance on solving the large bone tissue repair problem to clinical medicine.
Owner:SHANGHAI UNIV
Inducer and induced differentiation complete medium for inducing mesenchymal stem cells into nerve cells
ActiveCN105062970ANo exclusionNo ethical issuesNervous system cellsSkeletal/connective tissue cellsAdditive ingredientNeuroblast
The invention belongs to the technical field of stem cell induction differentiation, particularly relates to an inducer and an induction medium, and discloses an inducer and induced differentiation complete medium for inducing mesenchymal stem cells into nerve cells. The inducer for inducing the mesenchymal stem cells into the nerve cells comprises resveratrol, icariin, hydrocortisone, VEGF, IGF-I and EPO. Every one thousand milliliters of the induced differentiation complete medium for inducing the mesenchymal stem cells into nerve cells comprises 30-50 micromoles of resveratrol, 5-10 micromoles of icariin, 5-10 micromoles of hydrocortisone, 10-20 micrograms of VEGF, 5-10 micrograms of IGF-I, 2-5 micrograms of EPO and the balance mesenchymal stem cell serum-free complete media. According to the induced differentiation complete medium for inducing the mesenchymal stem cells into the nerve cells, the traditional Chinese medicine ingredients of resveratrol and icariin are combined with hydrocortisone and the growth factors of VEGF, IGF-I and EPO to synergistically induce the mesenchymal stem cells to be directionally differentiated into the nerve cells, and the selected induction components are all free of poison, high in induction efficiency, short in induction time, good in activity of the nerve cells obtained through induction, free of rejection after cell transplantation, free of ethical issues and high in safety.
Owner:QINGDAO RESTORE BIOTECHNOLOGY CO LTD
Method for differentiating induced pluripotent stem cells into mesenchymal stem cells
The invention discloses a method for differentiating induced pluripotent stem cells (iPSC) into mesenchymal stem cells (MSC). The method includes the steps that the induced pluripotent stem cells formed through induction are dissociated into single cells; the single cells are cultivated in a cell plate coated with I type collagen through an iPSC medium and one differential medium including platelet-derived growth factors, and then cultivated in another differential medium to obtain MSC-like cells; and finally, the MSC-like cells are maturated in an MSC medium to obtain the MSC. According to the induction approach, the iPSC is directly differentiated into the MSC, and the method has the beneficial effects of being short in period, high in efficiency, safe, stable and the like.
Owner:GUANGDONG XTEM BIOTECH CO LTD
Inducer for inducing human adipose derived stromal cells into insulin-secretion cells, induction medium and method
InactiveCN103756954AGenetic change noNo riskArtificial cell constructsSkeletal/connective tissue cellsInsulin Secreting CellRHODIOLA ROSEA ROOT
The invention belongs to the field of biomedicine, and relates to an inducer for inducing human adipose derived stromal cells into insulin-secretion cells, an induction medium and a method. The inducer for inducing human adipose derived stromal cells into insulin-secretion cells consists of the following components: a rhodiola rosea injection, nicotinamide, taurine and GLP-1. Every 1,000ml of the induction medium containing the inducer comprises 20-40g of the rhodiola rosea injection, 0.97-1.47g of the nicotinamide, 0.25-0.50g of the taurine, 26.84-40.27mg of the GLP-1 and the balance being a human adipose derived stromal cell serum-free medium, which are mixed uniformly, filtered and degermed. According to the inducer for inducing human adipose derived stromal cells into insulin-secretion cells, the induction medium and the method, differentiation of the human adipose derived stromal cells into the insulin-secretion cells is induced by using the injection of a traditional Chinese medicine, namely, rhodiola rosea, and each component is safe and nontoxic. The method has few steps and is short in time and high in the inducing efficiency.
Owner:青岛瑞思科生物科技有限公司
Method for inducing human mesenchymal stem cells differentiation to oligoden drocyte
The invention discloses a method for inducing differentiation from human mesenchymal stem cells to oligodendrocyte. The invention aims to provide a method for obtaining a large quantity of oligodendrocyte and using the oligodendrocyte to treat diseases such as myelin sheath damage or demyelination. The method is to obtain a large quantity of the autogenous human mesenchymal stem cells by a simple platy adhesion method, and to use adhesion molecules F3 / Contactin to induce differentiation from the human mesenchymal stem cells to the oligodendrocyte. The method adopts an easy, simple and convenient means to perform in vitro regulation on differentiation from the human mesenchymal stem cells to the oligodendrocyte, and achieves the aims of acquisition of the autogenous oligodendrocyte, preparation of a large quantity of the autogenous oligodendrocyte, and low cost. The method realizes the aim of applying the oligodendrocyte from the autogenous human mesenchymal stem cells to treat the demyelination disease, and has better economic benefit and social benefit.
Owner:INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
Method for directional induction of differentiation of mesenchymal stem cells (MSCs)
InactiveCN103146642ASimple stepsImprove induction efficiencyArtificially induced pluripotent cellsNon-embryonic pluripotent stem cellsCuticlePolycarbonate
The invention discloses a method for the directional induction of the differentiation of MSCs. The method for the directional induction of the differentiation of MSCs comprises the following steps: inoculating and adsorbing in-vitro induction cells to the lower surface of a polycarbonate film, inoculating in-vitro MSCs to the upper surface of the polycarbonate film, and co-culturing the induction cells and the MSCs to promote the differentiation of the MSCs to target cells, wherein each of the MSCs and the induction cells cannot pass through the holes of the polycarbonate film. The differentiation of UCMSCs to cuticle cells has a beneficial effect on the clinic skin regeneration research progress in the future. The method develops ideas for the increase of the induction method for the multi-directional differentiation of the MSCs, and provides bases for the construction of tissue engineering skins through adopting the UCMSCs as seed cells.
Owner:FIRST HOSPITAL AFFILIATED TO GENERAL HOSPITAL OF PLA
Application of magnetic nano materials in promotion of mesenchymal stem cell osteogenic differentiation
The invention belongs to the technical field of promotion of the mesenchymal stem cell osteogenic differentiation, and discloses application of magnetic nano materials in promotion of the mesenchymal stem cell osteogenic differentiation. The magnetic nano materials are active ingredients and have the function of promoting a mesenchymal stem cell to differentiate into an osteoblast. Toxicity tests prove that nano ruthenium magnetic nano materials nano-modified by ferric oxide, and the nano selenium magnetic nano materials nono-modified by the ferric oxide are almost nontoxic for the mesenchymal stem cell; alizarin red and oil red O dying proves that the magnetic nano materials prepared have the functions of promoting the mesenchymal stem cell osteogenic differentiation, and inhibiting adipogenic differentiation; the prepared magnetic nano materials such as the nano ruthenium or nano selenium nano-modified by the ferric oxide, are nano-coupled with the ferric oxide in excessive amount directly, and no other auxiliary reagent is needed for the preparation process, the product system is simple, and a product can be preserved and used directly.
Owner:JINAN UNIVERSITY
Culture method for inducing mesenchymal stem cell to differentiate for forming osteocyte under condition without CO2
InactiveCN102586181ADoes not affect activityNormal growthSkeletal/connective tissue cellsSodium bicarbonateVitamin C
The invention provides a culture method for inducing mesenchymal stem cell to differentiate for forming osteocyte under the condition without CO2. The culture method comprises the following steps of: culturing the third-generation human mesenchymal stem cell under the conditions of 5 percent of CO2 and 37 DEG C and standing overnight; after the cell is completely attached to the wall and grows until 80 percents of cell is fused, replacing an inductive culture medium and culturing under the conditions of 37 DEG C without CO2 for 14-21 days to obtain osteoblast, wherein the inductive culture medium is formed by adding fetal calf serum, penicillin, streptomycin, hexadecadrol, beta-sodium glycerophosphate and vitamin C in a basal medium and is prepared from the following components calculatedby final concentration: 0.41g / L of sodium bicarbonate, 1.5g / L of disodium hydrogen phosphate dodecahydrate, 0.07g / L of monopotassium phosphate and 8.17g / L of sodium chloride; and solvent of the inductive culture medium is an L-DMEM cell culture medium free of sodium bicarbonate. According to the novel culture medium formula provided by the invention, the mesenchymal stem cell osteoblast can be normally induced to differentiate; the differentiation effect is favorable, so that the consumption of equipment is reduced; and the novel culture medium formula is suitable for cell culture and research on induced differentiation under the condition without CO2.
Owner:ZHEJIANG UNIV
Differentiation of mesenchymal stem cells into fibroblasts, compositions comprising mesenchymal stem cell-derived fibroblasts, and methods of using the same
ActiveUS20130121973A1Prevent rejectionReduce riskBiocideArtificial cell constructsWound healingFibroblast
Methods and compositions are provided for the differentiation and characterization of mammalian fibroblast from mesenchymal stem cells. The methods of the invention provide a means to obtain mesenchymal stem cell-derived fibroblast populations, e.g., seeded on a scaffold, which may be used in wound healing.
Owner:ESCAPE THERAPEUTICS INC
Differentiation of mesenchymal stem cells into fibroblasts, compositions comprising mesenchymal stem cell-derived fibroblasts, and methods of using the same
ActiveUS20110081326A1Avoid immune rejection issuePromote wound healingBiocideMammal material medical ingredientsWound healingFibroblast
Methods and compositions are provided for the differentiation and characterization of mammalian fibroblast from mesenchymal stem cells. The methods of the invention provide a means to obtain mesenchymal stem cell-derived fibroblast populations, e.g., seeded on a scaffold, which may be used in wound healing.
Owner:ESCAPE THERAPEUTICS INC
Genetically modified stem cell for cartilage repair treatment and application thereof
PendingCN113774028AReduce or avoid local inflammationPromote differentiationVirusesPeptide/protein ingredientsInflammatory factorsPharmaceutical drug
The invention discloses a genetically modified stem cell for cartilage repair treatment as well as a preparation method and application of the genetically modified stem cell, and further provides a pharmaceutical composition containing the stem cell. The stem cells comprise nucleic acid for coding an anti-inflammatory factor and nucleic acid for coding a cartilage repair factor, by simultaneously modifying the mesenchymal stem cells by adopting the anti-inflammatory factor and a factor for promoting cartilage differentiation, the mesenchymal stem cells can be effectively promoted to be differentiated into cartilage, and by comprehensively acting on various immune cells through combination of various factors, and the activity of various immune cells is inhibited, so that the local inflammatory environment problem of cartilage repair of parts such as bones and knees is solved, and the swelling condition of a clinical patient is reduced or decreased when the mesenchymal stem cells are applied to bone and knee repair.
Owner:BEIJING JIYUAN BIOTECH
Method for differentiating mesenchymal stem cell into neural cell and pharmaceutical composition containing the neural cell for neurodegenerative disease
InactiveUS20070054399A1Easy to distinguishCarry-out quicklyNervous disorderMuscular disorderNeural cellNeuroblast
The present invention provides a method of differentiating and proliferating a mesenchymal stem cell into the neural cell by culturing in a medium comprising an epidermal growth factor and a hepatocyte growth factor after confluent culture of the mesenchymal stem cell. The present invention provides more effective method of differentiating and proliferating the mesenchymal stem cell or the mononuclear cell comprising the mesenchymal stem cell into the neural cell with a neuron and an astrocyte in terms of time, efficiency and maturity as compared with conventional methods.
Owner:HYUN SOO KIM +2
Application of raloxifene in mesenchymal stem cell in-vitro osteoblast differentiation
InactiveCN102373175AHigh differentiation efficiencyHigh expressionOrganic active ingredientsSkeletal disorderDiseaseBone tissue engineering
The invention belongs to the technical field of cytobiology and bone tissue engineering, relates to a compound used for treating osteoporosis, and especially relates to an application of raloxifene in mesenchymal stem cell in-vitro osteoblast differentiation. According to the invention, raloxifene is added during a mesenchymal stem cell in-vitro osteoblast differentiation culturing process. With the application, mRNA and protein level expressions of osteoblast differentiation marker genes ALP and BMP2, and calcium ion sedimentation can be increased; and mesenchymal stem cell differentiation towards osteoblast can be promoted. The invention relates to the application of raloxifene in mesenchymal stem cell in-vitro osteoblast differentiation, and in treatments of diseases such as fractures,bone defects, and bone wounds.
Owner:NANJING UNIV
Preparation method of gradient mineralized bone extracellular matrix material
ActiveCN110227182AGood regeneration and repair effectEfficient regenerationTissue regenerationProsthesisPorosityBiomechanics
Owner:ZHEJIANG DISAI BIOTECHNOLOGY CO LTD
Culture medium for promoting differentiation of mesenchymal stem cells
ActiveCN111073850APromote differentiationImprove the efficiency of osteogenic differentiationMaterial nanotechnologyCarbon compoundsSomatotropic hormonePancreatic hormone
The invention relates to the field of stem cells, provides a culture medium for promoting differentiation of mesenchymal stem cells, and is used to solve the problem of low differentiation efficiencyof stem cells into bone cells. The culture medium for promoting differentiation of mesenchymal stem cells provided by the invention comprises 6-8 [mu]g / ml of insulin, 0.03-0.05 [mu]g / ml of transferrin, 0.1-0.3 ng / ml of BMP-4, 8-10 ng / ml of FGF-2, 4-6 ng / ml of growth hormone, 160-200 [mu]g / ml of glutathione, 0.5-2 mM of L-glutamine, 60-80 [mu]M of beta-mercaptoethanol, 0.1-0.5 mM of sodium pyruvate, 0.1-0.15 mM of non-essential amino acids, 10-15 [mu]M of nano ferric oxide, and the balance a DMEM culture medium. No biological serum is added into the culture medium, and a nano oxide is introduced at the same time, so that the culture medium can significantly promote differentiation of the mesenchymal stem cells into bone cells.
Owner:朗姿赛尔生物科技(广州)有限公司
Method for differentiating mesenchymal stem cell into neural cell and pharmaceutical composition containing the neural cell for neurodegenerative disease
InactiveUS7635591B2Carry-out quicklyIncrease the rate of differentiationNervous disorderMuscular disorderNeural cellHepatocyte growth factor
Owner:HYUN SOO KIM +2
Method for inducing human mesenchymal stem cells to differentiate into insulin-secreting cells in vitro
InactiveCN102618500AHigh transfection efficiencyImprove securityVector-based foreign material introductionForeign genetic material cellsProgenitorInsulin Secreting Cell
The invention relates to the field of biomedicine, in particular to a method for inducing human mesenchymal stem cells to differentiate into insulin-secreting cells in vitro, which includes, according to the technical scheme, constructing recombinant eukaryotic expression plasmids of SRF (serum response factor) gene; preliminarily inducing human mesenchymal stem cells into nestin cells first; further inducing the nestin cells into pancreatic progenitor cells; transfecting an eukaryotic expression vector containing the SRF gene to the cells above; and finally inducing the cells into insulin-secreting cells, wherein the eukaryotic expression vector comprises pEGFP (plasmid enhanced green florescence protein), pcDNA or pCMV. By the method, new seed cell sources for cell therapy of diabetes mellitus are provided, new theoretical and experimental basis is provided for directed induction of mesenchymal stem cells differentiating into insulin-secreting cells and clinical application of the insulin-secreting cells, and new models for drug development and screening related to the insulin-secreting cells are provided.
Owner:TIANJIN UNIV OF SCI & TECH
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