25 results about "Mesenchymal stem cell differentiation" patented technology

The invention relates to the technical field of stem cells, in particular to a serum-free induction medium with definite components for promoting and inducing mesenchymal stem cells to be differentiated into dermal papilla cells and an induction method. The small molecule compound composition is prepared from all-transretinoic acid, calomel, CHIR-99021 and minoxidil according to the molar ratio of (0.2 to 5): (2 to 50): (0.2 to 10): (0.2 to 10). According to the scheme, a plurality of small molecule compounds are adopted and have a synergistic effect, so that differentiation and function improvement of dermal papilla cells are promoted, and transformation of mesenchymal stem cells to the dermal papilla cells is promoted. The former three promote dermal papilla cell differentiation, and the latter promotes dermal papilla cell proliferation and inhibits apoptosis. According to the technical scheme, the technical problems that cell factors need to be added in the process of forming dermal papilla cells from stem cells through induced differentiation, so that the process cost is high, and the clinical safety is not ideal can be solved, the induction process is accelerated, and the method has ideal popularization and application prospects.

The present invention discloses the use of Avn Anthramides C (Avn C) as an inducer of chondrocytic differentiation in Mesenchymal Stem Cells (MSCs), providing a promising approach for regenerative cartilage therapy. By employing Avn C treatment, MSCs exhibit enhanced chondrocytic differentiation and elevated expression of key specific chondrogenic extracellular matrix (ECM) factors, notably Collagen Type II (COL2A1). Furthermore, when Avn C is used in combination with Chondroitin Sulfate (CS), a synergistic effect is observed, leading to upregulation of the transcriptional activity state of SOX9, a key regulator of chondrogenesis. The disclosed invention shows significant improvements in mRNA gene expression (ACAN, LRP1, SOX9), cellular protein levels (COL2A1, SOX9, LRP1), and functional outcomes, as evidenced by the secretion of extracellular matrix proteins (FSTL-1, BGH3, CO1A1, CO1A2, and CO3A1).

This invention discloses a method for preparing a 3D-printed hydrogel scaffold for cartilage repair, comprising: providing methacrylamide chitosan; providing 2-([1,1-biphenyl]-4-ylcarbamoyl)benzoic acid@polylactic acid-glycolic acid copolymer microspheres; obtaining a 3D-printed bio-ink for cartilage repair using methacrylamide chitosan, 2-([1,1-biphenyl]-4-ylcarbamoyl)benzoic acid@polylactic acid-glycolic acid copolymer microspheres, recombinant collagen CF-1552, and lithium magnesium silicate as main raw materials; and obtaining a 3D-printed bio-ink for cartilage repair using the 3D-printed bio-ink for cartilage repair as a raw material to obtain a 3D-printed hydrogel scaffold for cartilage repair. This 3D-printed hydrogel scaffold has advantages such as antibacterial properties and the ability to promote the differentiation of human bone marrow mesenchymal stem cells (hBMSCs) into chondrocytes, and can be used for cartilage repair, potentially bringing new curative opportunities for cartilage injury diseases.

The application discloses a culture solution for indirectly inducing human induced pluripotent stem cells into mesenchymal stem cells, which is composed of a neuroectoderm induction culture solution, a mesenchymal stem cell differentiation culture solution and a mesenchymal stem cell maintenance culture solution. The neuroectoderm induction culture solution is composed of a Neurobasal culture medium, a DMEM / F12 culture medium, an N2 additive, a B27 additive, a GSK3 inhibitor and a TGF-beta inhibitor. The application further discloses a method for indirectly inducing human induced pluripotent stem cells into mesenchymal stem cells, which comprises the following steps: taking the induced pluripotent stem cells, culturing the induced pluripotent stem cells in the neuroectoderm induction culture solution for 6 days, then culturing the induced pluripotent stem cells in the mesenchymal stem cell differentiation culture solution for 7 days, and then culturing the induced pluripotent stem cells in the mesenchymal stem cell maintenance culture solution for 3-5 generations. The method can direct the induced pluripotent stem cells to differentiate into mesenchymal stem cells in a short time, and greatly shortens the culture time.

To provide osteoblasts and compositions comprising the osteoblasts for treating bone diseases.SOLUTION: Disclosed is an osteoblast having both proliferation ability and differentiation ability, where the osteoblast has higher expression levels of connexin 43, RUNX2 and collagen type 1A compared with those of undifferentiated stem cells and mature osteocytes; higher expression levels of angiopoietin and alkaline phosphatase compared with those of undifferentiated stem cells; and lower expression levels of osterix, osteocalcin and osteopontin compared with those of mature osteocytes. Also, the osteoblast has a lower Ki-67 expression level compared with that of undifferentiated stem cells.SELECTED DRAWING: Figure 1

Provided is a method for inducing differentiation into nerve cells, wherein: a) mesenchymal stem cells are cultured for 1-10 days in a stem cell culture medium that is constituted by containing 60.0-90.0 vol% of a basal medium and 10.0-40.0 vol% of physiological saline and being further supplemented with 1.0-100.0 ng / mL of EGF, 0.2-20.0 ng / mL of FGF-2, 0.2-20.0 ng / mL of PDGF, and 0.5-8.0 mM of magnesium ascorbyl phosphate; b) thereafter, a protein component in a culture solution that has been obtained by the culturing are precipitated with an organic solvent; and c) using a medium that contains a cell-produced protein (CPPs) composition obtained by isolating a precipitate portion and dissolving the precipitate portion in physiological saline or a basal medium, the mesenchymal stem cells seeded at a density of 3×104 to 2×105 cells / mL are cultured at 4-10°C for 8 hours to 2 days, thereby inducing differentiation of the mesenchymal stem cells into nerve cells.

The application provides a method for improving the content of cartilage glycosaminoglycan by using cationic polymer, and the method promotes the content of glycosaminoglycan in cartilage tissue to be obviously increased by treating cartilage with a preparation containing polybrene, creates a good microenvironment for cartilage repair, and promotes the cartilage to be better repaired and regenerated; the method can improve the content of glycosaminoglycan in the differentiated tissue of mesenchymal stem cells by culturing the mesenchymal stem cells in an induction medium containing polybrene, promotes the mesenchymal stem cells to be better differentiated into cartilage, and has a wide clinical application prospect.

The invention relates to the technical field of biomedicine, in particular to a mesenchymal stem cell differentiation method for promoting bone joint repair, which comprises the following steps: inoculating mesenchymal stem cells on a three-dimensional scaffold material to form a complex, applying an alternating electric field within a specific parameter range for stimulation, and combining with growth factor culture to realize directional differentiation. According to the method, by regulating and controlling the frequency, the voltage amplitude and the action time of the alternating electric field, the differentiation efficiency of the cells in the cartilage or osteogenesis direction is remarkably improved, and meanwhile the adaptability of the cells in the complex bone joint microenvironment is enhanced.

The invention relates to the technical field of biological cell culture, and the biological cell culture medium comprises a basic nutrient component, a signal regulation factor, a differentiation promoting factor and an auxiliary stable factor, and the unit of each component is mg / L according to the total volume of the culture medium; the basic nutrition component is prepared from the following substances: 200 to 800 L of glutamine, 150 to 600 L of serine, 300 to 1200 L of proline and 100 to 400 L of lysine hydrochloride. The invention relates to the technical field of biological cell culture. According to the mesenchymal stem cell induced chondrogenic differentiation culture medium and the preparation method thereof, by optimizing the composition of the culture medium, a specific activity enhancing component and a signal regulating component are selectively introduced, so that the efficiency of differentiating mesenchymal stem cells into chondrocytes is remarkably improved; an amino acid derivative in the activity enhancing component can effectively promote cell metabolism activity, and a growth factor simulant and a signal channel regulator in the signal regulating component synergistically act on a key signal channel in the cell differentiation process.

A method for mesenchymal stem cell differentiation from human pluripotent stem cells is provided, particularly including the step of adaptation of embryoid bodies from 3-dimensional culture to 2-dimensional culture. The method enables rapid mesoderm and mesenchymal stem cell transformation by transferring from 3-dimensional culture to 2-dimensional culture. Also, the method relates to obtaining mesenchymal stem cell, which can be used in clinical applications, with this method.

The invention relates to the technical field of biological culture, and particularly discloses a culture medium for improving synthesis of unsaturated fatty acid in cell culture fat, which is used for solving the problem that the content of unsaturated fatty acid in the culture fat is not satisfactory. The differentiation culture medium comprises the following components: a basic culture medium, a specific porcine adipose-derived mesenchymal stem cell differentiation induction factor and an unsaturated fatty acid additive. Wherein the specific induction factors comprise but are not limited to factors such as insulin, dexamethasone, indometacin, 3-isobutyl-1-methylxanthine, rosiglitazone and the like. The unsaturated fatty acid additive comprises alpha-linolenic acid, and by adding the substances into the culture medium, the differentiation efficiency of the porcine adipose-derived mesenchymal stem cells and the directional synthesis of the unsaturated fatty acid in the cells can be improved. According to the improved differential culture medium, cell differentiation and unsaturated fatty acid synthesis are effectively promoted by optimizing components and proportions, and the improved differential culture medium has an important application prospect.

The invention provides a preparation method of adipose-derived stem cells and application of the adipose-derived stem cells in neural restoration, and further, a specific differentiation-promoting peptide CF-8 for promoting differentiation of adipose-derived stem cells into neuron-like cells is screened from pilose antler polypeptides. The neuron-like cells generated by differentiation after the action of the differentiation promoting peptide have a good treatment effect on traumatic brain injury, and the application prospect is good.

The invention relates to the technical field of islet cell culture, and discloses a method for inducing mesenchymal stem cells to be differentiated into islet-like cells and application of the islet-like cells, and the method comprises the following steps: inoculating the mesenchymal stem cells into an umbilical cord-based tissue cell culture medium A for adherent multiplication culture; preparing the amplified mesenchymal stem cells into a cell suspension, and aggregating the cells into balls through centrifugation or limited culture; carrying out induced culture on the mesenchymal stem cell spheres by using a culture medium B; carrying out induced culture on the started cell balls by using a culture medium C; and carrying out induced culture on the differentiated cell balls by using a culture medium D to obtain the islet-like cells. Mesenchymal stem cells are prepared into cell spheres to enhance the intercellular interaction, and a culture medium B containing activin A and all-transretinoic acid, a culture medium C containing high glucose and nicotinamide and a culture medium D containing trichostatin A and Exendin-4 are used for staged induction to obtain the islet-like cells with the insulin secretion function.

The present application relates to the technical field of stem cells, and particularly relates to a serum-free induction medium with clear components for promoting differentiation of induced mesenchymal stem cells into dermal papilla cells and an induction method. A small molecule compound composition comprises all-trans retinoic acid, forskolin, CHIR-99021 and minoxidil in a molar ratio of 0.2-5:2-50:0.2-10:0.2-10. The present application adopts multiple small molecule compounds to promote differentiation and function improvement of dermal papilla cells and transformation of mesenchymal stem cells into dermal papilla cells. The first three promote differentiation of dermal papilla cells, and the last one promotes proliferation and inhibits apoptosis of dermal papilla cells. The present application can solve the technical problems of high process cost and unsatisfactory clinical safety caused by the need to add cytokines in the process of inducing differentiation of stem cells into dermal papilla cells, and speeds up the induction process, and has an ideal application prospect.

The invention discloses an electroactive bone repair membrane material with a bone-imitating unit structure as well as a preparation method and application of the electroactive bone repair membrane material. Research shows that the bioactivity of the bone repair membrane material can be improved by forming a bone-imitating unit structure on the electroactive bone repair membrane material. The electroactive bone repair membrane material with the imitated bone unit structure can effectively promote mesenchymal stem cell differentiation and up-regulate expression of tissue repair related genes, and can promote vascularized bone regeneration in vivo through an osteogenesis-vascularization synergistic effect.

The application provides a preparation method of fat stem cells and the use thereof in nerve repair, and further, a specific differentiation-promoting peptide CF-8 for promoting differentiation of fat mesenchymal stem cells into nerve-like cells is screened from a deer antler polypeptide, wherein the nerve-like cells differentiated after the action of the differentiation-promoting peptide have a good therapeutic effect on treating traumatic brain injury, and have a good application prospect.

The present disclosure belongs to the field of biological medicines, and relates to an inducer for inducing differentiation of mesenchymal stem cells into estradiol-secreting cells. The inducer for inducing differentiation of mesenchymal stem cells into estradiol-secreting cells uses human mesenchymal stem cell serum-free culture medium as a substrate and comprises the following components in mass concentration ratios: 20-60 mg / L of bone morphogenetic protein-4, 20-60 mg / L of bone morphogenetic protein-7, 2-8 mg / L of retinoic acid, 2-8 mg / L of resveratrol, 2-8 mg / L of icariin, 2-8 μg / L of benzamide, 2-8 μg / L of chloroplatinic acid hexahydrate, 2-8 μg / L of ethanolamine, 2-10 μg / L of erythropoietin and 2-10 μg / L of vascular endothelial growth factor. The inducer for inducing differentiation of mesenchymal stem cells into estradiol-secreting cells provided by the present disclosure has a high induction efficiency.

The invention discloses a culture medium for promoting differentiation of mesenchymal stem cells and a culture method, and belongs to the technical field of biology. The culture medium comprises a basic culture medium, beta-nicotinamide mononucleotide (0.3 to 6.0 mM), atractylenolide II (3 to 10 nM), BMP-4 (1 to 5 ng / mL) and glutathione (50 to 100 [mu] g / mL). Osteogenic differentiation related signal pathways are activated through the synergistic effect of multiple factors, and the differentiation efficiency is remarkably improved to 85% or above. Experiments show that the culture medium has a remarkable effect in bone marrow-derived mesenchymal stem cells, the calcification area is increased by 19.9% compared with that of a basic culture medium, the problems of low efficiency and high cost of a single inducible factor in the prior art are solved, and the culture medium is suitable for bone tissue engineering and clinical bone defect repair and has wide clinical application value.

A method for preparing mesenchymal stem cells (MSCs) is provided. The method includes providing expanded potential stem cells or a first cell culture including the expanded potential stem cells; culturing the expanded potential stem cells or the first cell culture in a trophoblast stem cell (TSC) differentiation medium to obtain trophoblast stem cells or a second cell culture including the trophoblast stem cells; culturing the trophoblast stem cells or the second cell culture in a mesenchymal stem cell (MSC) differentiation medium to obtain mesenchymal stem cells or a third cell culture including the mesenchymal stem cells; and passaging the mesenchymal stem cells or the third cell culture in an MSC expansion medium while maintaining characteristics of the mesenchymal stem cells.

The invention discloses a cell culture device for regulating and controlling adipose tissue-derived stem cell differentiation by miR-204-5p, and relates to the technical field of cell culture, the cell culture device for regulating and controlling adipose tissue-derived stem cell differentiation by miR-204-5p comprises a culture box body, a protective door is hinged to the front side of the culture box body, and a culture cavity is formed in the culture box body. In the process of culturing the adipose-derived mesenchymal stem cells by using the culture device, through mutual cooperation of the feeding assembly, the discharging assembly, the wall-adhering assembly and the control assembly, culture medium liquid is conveyed in a rotating and wall-adhering mode in the liquid-changing culture process, disturbance to original culture liquid is effectively reduced, and the culture efficiency is improved. According to the device, the wall-attached cells are prevented from being pulled by mechanical force generated in the liquid dropping process, stable cell culture is facilitated, meanwhile, the flowing-out culture medium liquid can uniformly flow to different positions on the peripheral side in the culture tank, the liquid changing uniformity is ensured, and the uniform culture of the cells is further ensured.

The invention discloses a method for inducing differentiation of uterine blood mesenchymal stem cells into hair follicle-derived melanocytes, which is characterized in that directional differentiation is realized by simulating hair follicle microenvironment conditions and adopting a two-stage induction strategy: in the first stage, the uterine blood mesenchymal stem cells are induced to be differentiated into hair follicle melanocytes; in the second stage, the hair follicle melanin stem cells are further induced to be differentiated into hair follicle-derived melanocytes. The hair follicle-derived melanocyte has dual application values: melanin can be synthesized, hair coloring can be realized after transplantation, and the hair follicle-derived melanocyte can be homed to skin for treating pigment disorder diseases. Researches prove that the T cell immune response of a vitiligo patient has no aggressiveness to hair follicle-derived melanocytes, so that the T cell immune response can become an ideal cell for transplantation treatment of vitiligo compared with skin-derived melanocytes. According to the method, the uterine blood mesenchymal stem cells are induced and differentiated into the hair follicle-derived melanocytes, and a new thought is provided for obtaining the hair follicle-derived melanocytes.

The present invention relates to a method of differentiating mesenchymal stem cells into cardiomyocytes. When the method of the present invention is used, cardiomyocytes may be obtained from mesenchymal stem cells with high efficiency. Therefore, the method of the present invention may be effectively used for treatment of heart diseases, such as heart failure, which require myocardial regeneration for treatment.