The embodiment of the invention discloses an autologous collagen-containing water needle and a preparation method thereof. The autologous collagen-containing water needle comprises the following components in parts by weight: 10-20 parts of autologous collagen, 10-20 parts of hyaluronic acid, 5-10 parts of biopolysaccharide glue, 5-10 parts of a sheep placenta extract, 3-8 parts of a levorotatoryC stock solution, 2-3 parts of a grape seed extract, 2-5 parts of fructan, 2-5 parts of beta-glucan, 1-5 parts of vitamin C, 2-5 parts of amino acid, 2-3 parts of an coenzyme, 2-4 parts of an anti-allergic agent, 2-5 parts of an antioxidant, 1-3 parts of a sun-screening agent and 3-8 parts of a moisturizer. The water needle is prepared from the autologous collagen extracted from human venous blood, is autologous, does not generate exclusion, contains a variety of nutrients required for cell repair and cell growth, and has relatively strong efficacies of accelerating wound healing of skin and mucosa, promoting the cell growth, promoting wound healing and strengthening the immune system.