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Induction medium and inducer for transformation of mesenchymal stem cells into testosterone secretory cells

A technology of testosterone-secreting cells and induction medium, applied in animal cells, vertebrate cells, bone/connective tissue cells, etc., can solve the problems of low induction efficiency, few sources, weak cell activity, etc., and achieve the efficiency of induction and differentiation High, no ethical issues, high safety effects

Active Publication Date: 2015-12-09
QINGDAO RESTORE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

3. Leydig cell transplantation: Although it can avoid the production of sperm, it is basically not restricted by ethics, but the source is too small, extraction and cultivation are difficult, and clinical application is limited
2. MSC and Leydig cells are co-cultured to induce testosterone-secreting cells. Leydig cells can secrete a variety of factors and hormones during the culture process. The whole induction process is very complicated, and the signal transduction pathway involved in the induction process is s

Method used

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  • Induction medium and inducer for transformation of mesenchymal stem cells into testosterone secretory cells
  • Induction medium and inducer for transformation of mesenchymal stem cells into testosterone secretory cells
  • Induction medium and inducer for transformation of mesenchymal stem cells into testosterone secretory cells

Examples

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Example Embodiment

[0020] Example 1 An inducer for transforming mesenchymal stem cells into testosterone secreting cells, containing: aspirin, dexamethasone, progesterone, resveratrol, insulin, parathyroid hormone, vitamin C, and cysteine.

Example Embodiment

[0021] Example 2 In the induction medium for transforming mesenchymal stem cells into testosterone secreting cells of this example, the following raw materials were dissolved according to their respective characteristics, and human mesenchymal stem cell serum-free medium was used as the substrate (LONZA, catalog number 00190632), Make the content of each component dilute to 1000ml as follows: aspirin (Sigma, A2093-100G) 2mmol, dexamethasone (Sigma, D4902-25MG) 0.1μmol, progesterone (Sigma, V900699-5g) 2μg, resveratrol (Sigma, R5010-100MG) 30μmol, insulin (Sigma, I0908) 10mg, parathyroid hormone (Sigma, P7036) 0.5nmol, vitamin C (Sigma, A5960-10mg) 2mmol, cysteine ​​(Sigma, 861677-10G) ) 0.4mmol.

Example Embodiment

[0022] Example 3 In the induction medium for transforming mesenchymal stem cells into testosterone-secreting cells of this example, the following materials were dissolved according to their respective characteristics, and human mesenchymal stem cell serum-free medium was used as the substrate (LONZA, catalog number 00190632), Make the content of each component as follows to dilute to 1000ml: aspirin (Sigma, A2093-100G) 1.5mmol, dexamethasone (Sigma, D4902-25MG) 0.2μmol, progesterone (Sigma, V900699-5g) 2.5μg, Tribulus terrestris Retrol (Sigma, R5010-100MG) 40μmol, insulin (Sigma, I0908) 20mg, parathyroid hormone (Sigma, P7036) 1nmol, vitamin C (Sigma, A5960-10mg) 3mmol, cysteine ​​(Sigma, 861677- 10G) 0.6mmol.

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Abstract

The invention belongs to the technical field of induced differentiation of stem cells and particularly relates to an induction medium and an inducer for transformation of mesenchymal stem cells into testosterone secretory cells. The inducer for transformation of the mesenchymal stem cells into the testosterone secretory cells is composed of aspirin, dexamethasone, progesterone, resveratrol, insulin, parathyroid hormone, vitamin C and cysteine. Each 1000ml of the induction medium which takes a human mesenchymal stem cell serum-free medium as a matrix comprises 1-2mmol of aspirin, 0.1-0.3micromole of dexamethasone, 2-3micrograms of progesterone, 30-50micrograms of resveratrol, 10-30mg of insulin, 0.5-1.5nmol of parathyroid hormone, 2-4mmol of vitamin C, 0.4-0.8mmol of cysteine and the rest of the human mesenchymal stem cell serum-free medium. The induction medium and the inducer for transformation of the mesenchymal stem cells into the testosterone secretory cells have the advantages that the induced differentiation efficiency is high; cell viability is high after induction, induced transformation of the mesenchymal stem cells into the testosterone secretory cells can be realized, and high safety is achieved.

Description

technical field [0001] The invention belongs to the technical field of induction and differentiation of stem cells, and in particular relates to a mesenchymal stem cell directional differentiation inducer and a culture medium. Background technique [0002] Testosterone, also known as testosterone, testosterone or testosterone, is a steroid hormone secreted by Leydig cells (Leydig cells) in men or ovaries in women, and a small amount of testosterone is also secreted by the adrenal glands. 95%, testosterone has the functions of maintaining muscle strength and quality, maintaining bone density and strength, refreshing and improving physical fitness in the body. About 11 days after the fetus is born, Leydig cells in the testis differentiate from their stem cells (stemLeydigcells, SLCs) to adult Leydig cells, at this time, 3β-hydroxysteroid dehydrogenase (3β-HSD) can be detected in the interstitial area of ​​the testis expression. 3β-HSD is one of the steroid hormone synthases ...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
Inventor 张炳强
Owner QINGDAO RESTORE BIOTECHNOLOGY CO LTD
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