Inducing agent and culture medium for transformation of adipose-derived stem cell into testosterone cell

Abstract

The invention belongs to the technical field of cell induced differentiation, and in particular relates to an inducing agent and a culture medium for directional differentiation of an adipose-derived stem cell. The inducing agent for transformation of an adipose-derived stem cell into a testosterone cell consists of icariin, retinoic acid and human chorionic gonadotropin. The inducing culture medium for transformation of the adipose-derived stem cell into the testosterone cell is prepared by a method as follows: each 1000ml of the inducing culture medium consists of 0.5-10umol of icariin, 1-10umol of retinoic acid and 20,000-80,000 units of human chorionic gonadotropin, as well as a human mesenchymal stem cell serum-free medium, and the culture medium is obtained through uniformly mixing, filtering and sterilizing. The inducing agent and the culture medium for transformation of the adipose-derived stem cell into the testosterone cell disclosed by the invention are free from cell transfection, so as to avoid risks of gene modification and cancer, and induced differentiation efficiency is high; the inducing agent and the culture medium develop characteristics of traditional Chinese medicine, and are capable of inducing transformation of the autologous adipose-derived stem cell into the testosterone cell, and are free from an ethics problem and high in safety.

Description

technical field [0001] The invention belongs to the technical field of stem cell induction and differentiation, and in particular relates to an induction agent and culture medium for adipose stem cell directed differentiation. Background technique [0002] Testosterone, also known as testosterone, testosterone or testosterone, is a steroid hormone secreted by Leydig cells (Leydig cells) in men or ovaries in women, and a small amount of testosterone is also secreted by the adrenal glands. 95%, testosterone has the functions of maintaining muscle strength and quality, maintaining bone density and strength, refreshing and improving physical fitness in the body. When the function of Leydig cells in human testis declines, the secretion of testosterone is insufficient, causing sexual dysfunction, loss of libido, fatigue, easy insomnia, and memory loss in young men. At present, there are three treatment methods for the decreased function of Leydig cells in human testis: 1. Traditi...

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Problems solved by technology

3. Leydig cell transplantation: Although it can avoid the production of sperm, it is basically not restricted by ethics, but the source is too small, extraction and cultivation are difficult, and clinical application is limited

2. Mesenchymal stem cells and Leydig cells are co-cultured to induce testosterone-secreting cells. Leydig cells can secrete a variety of factors and hormones during the culture process. The entire induction process is very complicated, and it is not yet possible to know exactly the signal transduction involved in the induction process. Guidance

4. Some researchers found that only RA can induce MSCs to secrete testosterone and estradiol, but there is no significant difference between the secretion amount of the experimental group and the control group in the literature

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