Application of magnetic nano materials in promotion of mesenchymal stem cell osteogenic differentiation

A magnetic nano- and stem cell technology, applied in the field of promoting osteogenic differentiation of mesenchymal stem cells, can solve problems such as troubles, and achieve the effects of inhibiting adipogenic differentiation, promoting osteogenic differentiation, and simple product system

Active Publication Date: 2014-10-15
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Stem cell transplantation to treat various diseases has become a hot spot in medical research today, but how to distinguish donor cells from recipients after transplantation and observe their survival and migration in vivo has always been a bottleneck that plagues its clinical application

Method used

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  • Application of magnetic nano materials in promotion of mesenchymal stem cell osteogenic differentiation
  • Application of magnetic nano materials in promotion of mesenchymal stem cell osteogenic differentiation
  • Application of magnetic nano materials in promotion of mesenchymal stem cell osteogenic differentiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: the preparation of the nanometer ruthenium of ferric oxide nanometer modification

[0038] 20mg γ-Fe 2 o 3 The nanoparticles were uniformly ground and dispersed in 160mL distilled water, and ultrasonically dispersed in distilled water. After 1 min, at a mechanical stirring speed of 170 r / min, 4 mL of RuCl with a concentration of 0.043 M was added dropwise 3 solution, after the dropwise addition was completed, then add 0.28g of tiopronin. It can be seen that the suspension gradually changes from red to brown. After the reaction was completed, it was washed three times with distilled water under the action of a magnetic field to remove unreacted ions and colloidal ruthenium particles generated. Resuspended in distilled water to obtain Fe 2 o 3Such as suspension. Centrifuge, wash with ultrapure water, and dry the product at 70°C for 2 days to obtain nano-selenium modified with ferric oxide. Observed by TEANAI-10 transmission electron microscope, the r...

Embodiment 2

[0039] Embodiment 2: the preparation of the nano-selenium of ferric oxide nano-modification

[0040] 20mg γ-Fe 2 o 3 The nanoparticles were uniformly ground and dispersed in 160mL distilled water, and ultrasonically dispersed in distilled water. After 1min, under the mechanical stirring speed of 170r / min, add 11.6mg and then add Na 2 SeO 3 , 0.029g tiopronin. It can be seen that the suspension gradually changes from light red to dark red. After the reaction is completed, wash with distilled water three times under the action of a magnetic field to remove unreacted ions and colloidal selenium particles. Resuspended in distilled water to obtain Fe 2 o 3 Se suspension. Centrifuge, wash with ultrapure water, and dry the product at 70°C for 2 days to obtain nano-selenium modified with ferric oxide. Its morphology was observed by TEANAI-10 transmission electron microscope, as shown in figure 1 Shown in B. Its particle size is 160-170nm. The nanoparticles can exist stably...

Embodiment 3

[0041] Example 3: Toxicity detection of magnetic nanomaterials on human umbilical cord mesenchymal stem cells

[0042] In this experiment, human umbilical cord mesenchymal stem cells (MSCs) purchased from ATCC were selected. The sample number tested is: Fe 2 o 3 Ru (nano-ruthenium modified with ferric oxide nanometers), Fe 2 o 3 Se (nano-selenium modified with ferric oxide nanometers) and sodium fluoride.

[0043] (1) MTT test method

[0044] The well-grown third generation human umbilical cord mesenchymal stem cells were taken, and the concentration of living cells was adjusted to 2×10 4 / mL was added to a 96-well culture plate, 100 μL per well, cultured in an incubator for 24 hours, and then added 100 μL of aqueous solutions of magnetic nanomaterials with different concentrations. The negative control was equal volume of normal saline, and the positive control was sodium fluoride. Four replicate wells were set up in both the sample addition group and the control group,...

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Abstract

The invention belongs to the technical field of promotion of the mesenchymal stem cell osteogenic differentiation, and discloses application of magnetic nano materials in promotion of the mesenchymal stem cell osteogenic differentiation. The magnetic nano materials are active ingredients and have the function of promoting a mesenchymal stem cell to differentiate into an osteoblast. Toxicity tests prove that nano ruthenium magnetic nano materials nano-modified by ferric oxide, and the nano selenium magnetic nano materials nono-modified by the ferric oxide are almost nontoxic for the mesenchymal stem cell; alizarin red and oil red O dying proves that the magnetic nano materials prepared have the functions of promoting the mesenchymal stem cell osteogenic differentiation, and inhibiting adipogenic differentiation; the prepared magnetic nano materials such as the nano ruthenium or nano selenium nano-modified by the ferric oxide, are nano-coupled with the ferric oxide in excessive amount directly, and no other auxiliary reagent is needed for the preparation process, the product system is simple, and a product can be preserved and used directly.

Description

technical field [0001] The invention belongs to the technical field of promoting osteogenic differentiation of mesenchymal stem cells, and in particular relates to the application of a magnetic nanometer material in promoting the osteogenic differentiation of mesenchymal stem cells. Background technique [0002] Stem cells are a type of cell with self-renewal ability and multi-directional differentiation potential. It is the first cell in the process of human newborn cells from proliferation, migration, differentiation to maturity. The study of stem cells has become one of the most challenging and attractive fields in biology. Stem cells can be divided into embryonic stem cells and adult stem cells according to their source. Mesenchymal stem cells (MSCs, mesenehymal stem cells) are one of the adult stem cells. As a type of adult stem cells with self-renewal ability, mesenchymal stem cells can differentiate into various cell types. It can not only differentiate into a vari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
Inventor 刘杰刘莹刘亚楠郑楚萍
Owner JINAN UNIVERSITY
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