66 results about "Bone marrow aspirate" patented technology

Methods of arthroscopic harvesting of bone marrow aspirate from bone joints and therapeutic application of the bone marrow aspirate in a bone joint. The method of harvesting includes extracting bone marrow from a joint, adding a biomaterial, and adding clotting agent to form a clot.

Various devices and methods may be provided to aspirate bone marrow from an associated bone using a powered drive and an aspiration needle or aspiration needle set. Such aspiration devices and methods may also be used in association with stem cell transplant procedures. The aspiration devices may include a coupler assembly, a containment bag or sterile sleeve, an ejector and / or an ejector funnel. An aspiration needle set may include a cannula and trocar with respective tips having optimum configurations, dimensions and / or orientations relative to each other to optimize penetration of a bone and / or bone marrow with minimum trauma to a patient. Exterior portions of some cannulas may include markings to indicate depth of penetration into associated bone marrow. Some cannulas may include one or more side ports for use during aspiration of bone marrow.

The present invention is directed to methods and compositions regarding the preparation of an cell concentrate, such as, for example, an osteogenic cell concentrate, from a physiological solution, such as bone marrow aspirate, blood, or a mixture thereof. In specific embodiments, the invention provides methods and compositions utilizing two physiological solution-processing techniques, particularly in a point of care environment, wherein centrifugation is not employed.

Invention embodiments described herein include methods and devices for stimulating mesenchymal stem cells in a stem cell source to differentiate into osteoblasts capable of forming bone. Devices and methods described include exposing a stem cell source, such as bone marrow aspirate, adipose tissue and / or purified allogenic stem cells, to an active agent, in a manner effective to form activated stem cells.

This invention is directed to a method for preparation of a biological sample for measurement of protein epitopes that allows for the preservation of intracellular protein epitopes and detection of signal transduction pathways based on the ability to capture transient activation states of the epitopes. The method provided by the invention allows for the rapid fixation of biological samples containing red blood cells, to ensure that epitopes of signal transduction molecules and other intracellular protein epitopes are preserved in the active state. The method of the invention further allows for lysis of red blood cells, thereby making it a useful method for cytometric analysis of biological samples, including, for example, whole blood, bone marrow aspirates, peritoneal fluids, and other red blood cell containing samples. The invention also provides a method to recover or “unmask” epitopes on intracellular antigens that have been made inaccessible by the cross linking fixative necessary to fix the sample. Significantly, the methods of the invention allow preservation and analysis of phospho-epitope levels in biological samples taken directly from patients to determine disease-specific characteristics.

The present invention is directed to methods and compositions regarding the preparation of an cell concentrate, such as, for example, an osteogenic cell concentrate, from a physiological solution, such as bone marrow aspirate, blood, or a mixture thereof. In specific embodiments, the invention provides methods and compositions utilizing two physiological solution-processing techniques, particularly in a point of care environment, wherein centrifugation is not employed.

Methods of arthroscopic harvesting of bone marrow aspirate from bone joints and therapeutic application of the bone marrow aspirate in a bone joint. The method of harvesting includes extracting bone marrow from a joint, adding a biomaterial, and adding clotting agent to form a clot.

A floating separating element for use in centrifugal separation of components of a physiological fluid comprises a positioning part and a separating part, where the positioning part is designed to automatically assume a position in a supernatant and a separating part is positioned at a desired location with respect to the interface between the supernatant and heavier components. In preferred embodiments the physiological fluids are blood or bone marrow aspirate, and the heavier components comprise red blood cells. The positioning part comprises the majority of the mass of the separating element and is thin so that differences in the position of the separating element with respect to the interface are small compared to differences in the densities of the separated compoments, particularly the component comprising red blood cells. A method allows red blood cells to move the separating element during decanting to ensure complete decant of the supernatant.

A method and apparatus for preparing a bone graft composite using an infusion chamber. A modular tube having a porous material contained therein and having removable end caps is provided. Bone marrow aspirate or other bone morphogenic protein containing suspensions may be infused into the tube. A filter on one end of the tube prevents the fluid from escaping while permitting air to be expelled from the tube as it is filled with bone marrow aspirate. Once infused into the tube, the bone marrow aspirate is allowed to settle to a putty or paste-like consistency, the putty and material together forming a bone graft composite.

Methods of arthroscopic resurfacing of anatomical tissue utilizing a biological component strengthened with glue or similar material. The biological component is selected from the group consisting of blood, blood components, PRP, bone marrow aspirate (BMA) and autologous conditioned plasma (ACP). The biological component / glue composition may be inserted (by injection and / or by employing a biologic resurfacing mold, for example) into, or in the vicinity of, the anatomical tissue. Upon insertion within, or contact with, the anatomical tissue, the biological component / glue composition is designed to coagulate and solidify (or partially solidify) within minutes, to advance healing or tissue growth of the anatomical tissue. The biological component / glue composition may optionally comprise components such as growth factors, antiseptic chemicals and / or antibiotics and / or electrolytes, or hormones or site-specific hybrid proteins, among others.

A perfusion device and method is provided. In one embodiment, the device includes a container having a first internal chamber configured to hold the material; a port for introducing the liquid into the chamber; a vent for releasing gas and liquid from the chamber; and a means for sealing the vent to allow a vacuum to be drawn on the first chamber The material may be biomaterial, such as a bone graft material in any form. In one embodiment, the container is a syringe that defines the internal material chamber and includes an end cap and a plunger The vent may be formed by a venting passageway in plunger and / or cap in some embodiments. In one embodiment, the vacuum may be created by a medical syringe coupled to the container, and which in some embodiments may also be used to deliver the liquid into the container The liquid may be bone marrow aspirate in some embodiments.

Devices for extracting bone marrow from a bone of a patient are disclosed. The device includes a housing defined in part by a cannula and a hollow needle disposed within the cannula. The hollow needle is movable axially relative to the cannula and extendable therefrom. The hollow needle is configured to aspirate bone marrow within a bone. A depth adjustment mechanism coupled to the hollow needle and the cannula is used to adjust the depth of the hollow needle relative to the cannula to control the distance of the needle within the bone. The device is attachable to an extraction device to extract bone marrow from the patient through the hollow needle. Methods of using these devices and methods of extracting bone marrow are also included.

Methods and therapeutic compositions for treating respiratory diseases, such as chronic obstructive pulmonary disease (COPD) and devices for administering the therapeutic compositions. Methods for treatment include producing a protein solution, producing a concentrated bone marrow aspirate (cBMA), optionally combining the protein solution and cBMA to form an therapeutic composition, and optionally saturating the autologous therapeutic composition with hydrogen gas, and administering the therapeutic composition to a subject in need thereof. The present methods, compositions and devices are useful for treating COPD and the progression of COPD.

Methods of arthroscopic resurfacing of a joint utilizing a biological component strengthened with fibrin glue. The biological component is selected from the group consisting of PRP, bone marrow aspirate (BMA) and autologous conditioned plasma (ACP). The biological component / fibrin glue composition may be inserted (by injection or by employing a biologic resurfacing mold, for example) into a transosseous tunnel in the vicinity of the defect to be repaired. Upon insertion at the defect site, the biological component / fibrin glue composition is designed to coagulate and solidify within few minutes, to advance the healing of the damaged tissue and tissue growth. The biological component / fibrin glue composition may optionally comprise components such as growth factors, antiseptic chemicals and / or antibiotics and / or electrolytes, or hormones or site-specific hybrid proteins, among others.

Methods of treating peripheral vascular disease comprising administering a protein solution site of a defect at least two proteins from the group IL-1ra, sTNF-RI, sTNF-RII, IGF-I, EGF, HGF, PDGF-AB, PDGF-BB, VEGF, TGF-β1, and sIL-1RII. The solution may also comprise white blood cells, platelets, concentrated bone marrow aspirate, and combinations thereof.

Bone marrow aspirate from which water and fat have been substantially removed.

A method of preparing a bone graft. Mixing bone marrow aspirate with an effective amount of a binding reagent that is capable of binding with red blood cells in the bone marrow aspirate. The bound red blood cells are aggregated. The aggregated bound red blood cells are separated from the bone marrow aspirate to provide a supernatant. At least a portion of the supernatant is associated with an osteoconductive matrix to form the bone graft.

Multi-component separation devices configured to separate components of a liquid sample by centrifugation are provided. Aspects of the separation devices include a container having a distal end and a proximal end and a buoy configured to be displaced along a longitudinal axis within the container where the buoy includes one or more sealed chambers. Also provided are methods of using the subject devices to separate components of a multi-component liquid sample such as whole blood, bone marrow aspirate or stromal vascular fraction as well as systems suitable for practicing the subject methods.

This invention is directed to a method for preparation of a biological sample for measurement of protein epitopes that allows for the preservation of intracellular protein epitopes and detection of signal transduction pathways based on the ability to capture transient activation states of the epitopes. The method provided by the invention allows for the rapid fixation of biological samples containing red blood cells, to ensure that epitopes of signal transduction molecules and other intracellular protein epitopes are preserved in the active state. The method of the invention further allows for lysis of red blood cells, thereby making it a useful method for cytometric analysis of biological samples, including, for example, whole blood, bone marrow aspirates, peritoneal fluids, and other red blood cell containing samples. The invention also provides a method to recover or ''unmask'' epitopes on intracellular antigens that have been made inaccessible by the cross linking fixative necessary to fix the sample. Significantly, the methods of the invention allow preservation and analysis of phospho-epitope levels in biological samples taken directly from patients to determine disease-specific characteristics.

Methods of arthroscopic resurfacing of anatomical tissue utilizing a biological component strengthened with glue or similar material. The biological component is selected from the group consisting of blood, blood components, PRP, bone marrow aspirate (BMA) and autologous conditioned plasma (ACP). The biological component / glue composition may be inserted (by injection and / or by employing a biologic resurfacing mold, for example) into, or in the vicinity of, the anatomical tissue. Upon insertion within, or contact with, the anatomical tissue, the biological component / glue composition is designed to coagulate and solidify (or partially solidify) within minutes, to advance healing or tissue growth of the anatomical tissue. The biological component / glue composition may optionally comprise components such as growth factors, antiseptic chemicals and / or antibiotics and / or electrolytes, or hormones or site-specific hybrid proteins, among others.

Compositions and methods for separating a sample of whole blood or bone marrow aspirate into a fraction rich in at least one of platelets and pluripotent cells are provided. A sample of whole blood can be centrifuged in a collection tube comprising a separator substance formulated to settle between the PRP fraction and the at least one other fraction. Preferably, centrifugation is completed without substantial activation of platelets. Optionally, the separator substance could be hardened to form a solid barrier that allows removal of all or substantially all of the platelets in the PRP fraction without remixing of the PRP fraction with the at least one other fraction. Transfer devices and methods are also provided in which a sterile sample can be transferred from a separation / preparation container (e.g., vacutainer) to a consumer or other container (e.g., dropper) while maintaining sterility of the sample.

Various devices and methods may be provided to aspirate bone marrow from an associated bone using a powered drive and an aspiration needle or aspiration needle set. Such aspiration devices and methods may also be used in association with stem cell transplant procedures. The aspiration devices may include a coupler assembly, a containment bag or sterile sleeve, an ejector and / or an ejector funnel. An aspiration needle set may include a cannula and trocar with respective tips having optimum configurations, dimensions and / or orientations relative to each other to optimize penetration of a bone and / or bone marrow with minimum trauma to a patient. Exterior portions of some cannulas may include markings to indicate depth of penetration into associated bone marrow. Some cannulas may include one or more side ports for use during aspiration of bone marrow.

This invention is directed to a method for preparation of a biological sample for measurement of protein epitopes that allows for the preservation of intracellular protein epitopes and detection of signal transduction pathways based on the ability to capture transient activation states of the epitopes. The method provided by the invention allows for the rapid fixation of biological samples containing red blood cells, to ensure that epitopes of signal transduction molecules and other intracellular protein epitopes are preserved in the active state. The method of the invention further allows for lysis of red blood cells, thereby making it a useful method for cytometric analysis of biological samples, including, for example, whole blood, bone marrow aspirates, peritoneal fluids, and other red blood cell containing samples. The invention also provides a method to recover or “unmask” epitopes on intracellular antigens that have been made inaccessible by the cross linking fixative necessary to fix the sample. Significantly, the methods of the invention allow preservation and analysis of phospho-epitope levels in biological samples taken directly from patients to determine disease-specific characteristics.

Methods and systems for delivering biological products such as bone marrow aspirate (BMA) to different surgical sites during surgery (for example, arthroscopic surgery). The biological product is encapsulated or contained within a containment system comprising a carrier such as collagen carrier. The containment system may be formed of water soluble polymers, either natural, synthetic or semisynthetic, provided into films that may be made or molded into various shapes and sizes, and that may be manipulated to confer specific properties (such as solubility or degradation rates according to a specific environment, for example) of such films. The water soluble films may be processed into capsules, packets or other containers.

Methods of arthroscopic resurfacing of a joint utilizing a biological component strengthened with fibrin glue. The biological component is selected from the group consisting of PRP, bone marrow aspirate (BMA) and autologous conditioned plasma (ACP). The biological component / fibrin glue composition may be inserted (by injection or by employing a biologic resurfacing mold, for example) into a transosseous tunnel in the vicinity of the defect to be repaired. Upon insertion at the defect site, the biological component / fibrin glue composition is designed to coagulate and solidify within few minutes, to advance the healing of the damaged tissue and tissue growth. The biological component / fibrin glue composition may optionally comprise components such as growth factors, antiseptic chemicals and / or antibiotics and / or electrolytes, or hormones or site-specific hybrid proteins, among others.

Methods of treating peripheral vascular disease comprising administering a protein solution site of a defect at least two proteins from the group IL-1ra, sTNF-RI, sTNF-RII, IGF-I, EGF, HGF, PDGF-AB, PDGF-BB, VEGF, TGF-β1, and sIL-1RII. The solution may also comprise white blood cells, platelets, concentrated bone marrow aspirate, and combinations thereof.

Methods and systems for collecting and processing bone marrow in a closed system are provided. The systems and methods include the use of a bone marrow aspiration needle and guide configured to puncture cortical bone. Tube assembly is in fluid flow communication with the bone marrow aspiration needle. A first syringe is in fluid flow communication with the tube assembly and the bone aspiration. A bone marrow aspirate collection container in fluid flow communication with the first syringe and the tube assembly. A second syringe is connected to the collection container, a bone marrow aspirate filter in fluid flow communication with the tube assembly to filter bone marrow aspirate from the first syringe into the collection container. At least one flow controller is configured to direct the flow of bone marrow aspirate between the aspiration needle, the first syringe and the collection container.