10 results about "Bone marrow aspirate" patented technology

A target needle assembly can include a needle for forming a tunnel in bone and a cannula with filters for extracting bone marrow and bone cells from the bone. The needle can be formed at the end of the cannula. The needle can be a separate piece inside the cannula. The cannula can have filters for draining bone marrow into a lumen of the cannula. The filters can be sized to filter bone particulate.

The present invention provides methods of detecting breast cancer or breast cancer cells comprising: (a) obtaining a liquid sample from a subject which sample comprises cells, microvesicles and / or exosomes; and (b) determining the presence of soluble or cell surface associated Cyr61 in the sample; wherein the liquid sample is selected from blood, and / or bone marrow aspirate and wherein the determination of the presence of Cyr61 comprises detection via ELISA.

This invention discloses a bone marrow cell paraffin block, its preparation method, and its application, belonging to the field of pathological detection technology. The preparation method includes the following steps: Red blood cell lysis: Bone marrow aspirate is taken, and a precipitate containing cells and sediment is separated. A red blood cell lysis agent is added to lyse the red blood cells, and a lysed precipitate containing unlysed cells and sediment is separated. Formation: The lysed precipitate is resuspended in formalin solution for fixation, the supernatant is removed, and the precipitate is resuspended in agar solution. The agar is cooled and solidified, and then fixed again in neutral formalin. Dehydration: The solidified agar block is dehydrated. Embedding: The dehydrated agar block is embedded in paraffin to form a paraffin block. The cell paraffin block prepared by this method can supplement the diagnosis when bone marrow smears or bone marrow biopsy samples are not ideally prepared.

The utility model discloses a bone marrow puncture suction appliance which is labor-saving in operation. The bone marrow puncture suction appliance comprises a needle tube; the tube sleeve is elastically connected to the needle tube in a sleeving mode, a fourth positioning block is arranged at the end of the tube sleeve, and the fourth positioning block is of a kidney-shaped or oval cylinder structure; a needle core; the needle cylinder is detachably connected to the needle tube and the tube sleeve in a clamped mode and comprises a cylinder body, a driven piston arranged in a liquid storage cavity of the cylinder body in a sliding mode, a pressing piston and a linkage rod, extracted bone marrow is stored between the driven piston and the inner bottom of the cylinder body, and the middle of the linkage rod is rotationally connected to the interior of the liquid storage cavity through a rotating shaft; the distance between the rotating shaft and the driven piston is smaller than that between the rotating shaft and the pressing piston. Compared with common related products, the size of the needle tube is designed, so that the needle tube can be conveniently inserted into the bone marrow of a patient and butted with the bone marrow of the patient; the needle tube and the tube sleeve are movable, so that the needle core is convenient to disassemble and assemble; bone marrow suction adopts pressing operation, so that severe pain of a patient caused by instrument shaking is reduced, and the bone marrow suction operation is more labor-saving.

The invention discloses a group of diagnosis, typing and prognostic markers for children Langerhans cell histocytosis, and belongs to the technical field of disease diagnosis. In the invention, an overall metabolic profile of bone marrow aspirates (BMA) of 34 children LCH patients is analyzed, and the patients are divided into three subtypes: single system diseases (SS), multi-system diseases (MS-RO-) without dangerous organ tiredness and multi-system diseases (MS-RO +) with dangerous organ tiredness. The metabonomics characteristics of children diagnosed as LCH are described, the metabolic characteristics of a multi-system LCH (MS-RO +) group with dangerous organs being tired before and after treatment are comprehensively researched, and important metabolic markers for molecular diagnosis, subtype classification, treatment evaluation and prognosis are provided for children patients suffering from LCH, especially high-risk LCH.

Apparatus, system, and method embodiments provide a fractionation device for the fractionation or separation of blood or bone marrow aspirate (BMA) into one or more component layers and the efficient collection of layers of interest. The fractionation device includes a generally cylindrical sidewall, a top wall connected to an upper portion of the sidewall, a bottom wall connected to a lower portion of the sidewall, and an interior partition. The interior partition includes a sloped portion contacting the sidewall and a vertical portion contacting the top wall.

A bone void filler preparation system that includes a processing vessel, a processing cover, a bone void filler preparation container and tubing. The processing vessel has a recess formed therein. The processing vessel is adapted to receive bone marrow aspirate. The processing cover includes an outer wall, a central wall member and a connection port. The outer wall has an upper edge and a lower edge. The central wall member extends inwardly from the outer wall intermediate the upper and lower edges of the processing cover. The central wall member has an aperture formed therein. The central wall member has a downwardly directed portion that defines an air-retaining region. The air-retaining region is closer to the outer wall upper edge than the aperture. The connection port is operably connected to the aperture. The processing cover is movable in the processing cover recess. The bone void filler preparation container has an inlet portion and an outlet port. The bone void filler preparation container is adapted to receive a bone void filler matrix therein. The tubing fluidly connects the processing cover connection port and the bone void filler preparation container inlet port.

The present invention provides improved devices for biopsy, aspiration, stem cell acquisition, and methods of using the same. The devices balance aspiration with concurrent infusion to manage changes in pressure at the site of biopsy. The present invention can be adapted for any biopsy, aspiration, or cell harvest procedure, including adipose tissue aspiration and bone marrow aspiration (BMA). In particular, the present invention limits patient pain, prevents blood contamination, and increases cell mobilization, such as improved stem cell yields with intraosseous (10) pharmacological mobilization of stem cells during a BMA procedure, and with improved stem cell yields using pharmacological mobilization of stem cells from fat. The pharmacological mobilization of cells allows the harvest of cells from a biopsy many fold larger than existing methods.

A method and apparatus for preparing a bone marrow aspirate (BMA) sample for detection of a targeted nucleic acid sequence including a first fixation step including contacting said BMA sample with a first fixation solution including formalin, and a second fixation step including contacting said BMA sample with a second fixation solution including methanol and acetic acid.