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Building method of Pre-revascularizational large biological bone scaffold of composite cell

A construction method and technology of bone scaffolds, applied in the field of biomanufacturing, can solve the problems of unsatisfactory prefabricated vascular network scaffolds and inability to perform cell assembly tests, etc., to achieve rapid repair, improve repair efficiency, and high flexibility

Active Publication Date: 2017-12-01
SHANGHAI UNIV
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the prefabricated vascular network scaffolds prepared by a single process cannot meet the requirements of tissue engineering for the multi-scale structure and vascularized size and shape of bone scaffolds, let alone carry out effective cell assembly experiments. Fabrication of bone scaffolds with prefabricated vascular networks by an assembly-integrated process is still in its infancy

Method used

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  • Building method of Pre-revascularizational large biological bone scaffold of composite cell
  • Building method of Pre-revascularizational large biological bone scaffold of composite cell

Examples

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Embodiment 1

[0029] In this example, see Figure 1~Figure 2 A method for constructing a pre-vascularized bulk biological bone scaffold of composite cells, comprising the steps of:

[0030]a. Preparation of gelatin loaded with human bone marrow mesenchymal stem cells as raw material for printing bone scaffolds and sodium alginate loaded with human umbilical vein endothelial cells as sacrificial material, gelatin and sodium alginate powder baked at high temperature and sterilized by ultraviolet light Pour into the cell culture medium, and prepare gelatin solution and sodium alginate solution with the concentrations that meet the conditions of macroscopic printing and electrohydrodynamic direct writing; respectively, the third-generation human bone marrow mesenchymal stem cells and the third-generation human umbilical cord The venous endothelial cells were taken out from the incubator, after trypsin digestion, centrifugation and other steps, the supernatant was discarded to obtain human bone ...

Embodiment 2

[0036] This embodiment is basically the same as Embodiment 1, especially in that:

[0037] In this embodiment, a method for constructing a pre-vascularized bulk biological bone scaffold of composite cells, the bone scaffold material adopts chitosan, comprising the following steps:

[0038] a. Preparation of chitosan loaded with human bone marrow mesenchymal stem cells as raw material for printing bone scaffolds and sodium alginate loaded with human umbilical vein endothelial cells as sacrificial material: chitosan and The sodium alginate powder was poured into the cell culture medium, and the chitosan solution and the sodium alginate solution with the concentrations meeting the conditions of macroscopic printing and electrohydrodynamic direct writing were respectively prepared; respectively, the third-generation human bone marrow mesenchymal stem cells under cultivation were and third-generation human umbilical vein endothelial cells were taken out from the incubator, digested...

Embodiment 3

[0044] This embodiment is basically the same as the previous embodiment, and the special features are:

[0045] In this embodiment, a method for constructing a prevascularized bulk biological bone scaffold of composite cells, the bone scaffold material is a mixture of gelatin and hydroxyapatite, including the following steps:

[0046] a. Preparation Use gelatin loaded with human bone marrow mesenchymal stem cells as raw material for printing bone scaffolds and sodium alginate loaded with human umbilical vein endothelial cells as sacrificial material, weigh hydroxyapatite, and the quality of gelatin and hydroxyapatite The ratio is 5:1, the gelatin and hydroxyapatite powder that has been baked at high temperature and sterilized by ultraviolet light are mixed and poured into the cell culture medium with sodium alginate powder respectively, and the concentrations are prepared to meet the requirements of macroscopic printing and electrohydrodynamics Gelatin solution and sodium algi...

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Abstract

The invention discloses a building method of a pre-revascularizational large biological bone scaffold of a composite cell which is used for the field of biological manufacturing. According to the building method of the pre-revascularizational large biological bone scaffold of the composite cell, composite biological 3D printing, electro-hydro dynamics direct writing, subtractive manufacturing and a cell assembly technology are adopted to build a functional pre-revascularizational network in the interior of the large biological bone scaffold to promote repairing of large bone defect. The key of the building method lies in the fact that sodium alginate which is a biological material and can be degraded in a body is adopted to serve as a sacrificial material, endothelial cells are loaded in the sodium alginate, and the sodium alginate is adhered to the surface of a prefabricated vessel network through migration of the endothelial cells. Meanwhile, the sodium alginate is degraded to form the prefabricated vessel network. In addition, human mesenchymal stem cells in a bone scaffold material are differentiated into osteoblasts, and synthesizes and secretes bone matrix under the mutual promotional effect of the endothelial cells, mineralization is conducted, so that the efficiency of bone tissue repair is improved, the functional problem of large bone tissue repair is solved, and the building method has important significance on solving the large bone tissue repair problem to clinical medicine.

Description

technical field [0001] The invention relates to a method for constructing a functional clinical large bone tissue repair containing cells, in particular to a method for constructing a composite cell prevascularized large bio-bone scaffold, which is applied in the technical field of biomanufacturing. Background technique [0002] High-energy trauma, bone tumors and other diseases often lead to bone defects, and autologous bone transplantation is the best way to treat them. However, autologous bone transplantation is extremely limited and increases pain, while allogeneic bone transplantation has immune reactions and many complications. Although human bones have a certain ability to regenerate and self-repair, the repair of large bone defects (inner diameter > 5mm) cannot rely solely on the self-healing ability of bone. Therefore, large bone injuries are still an unsolved global problem as a common clinical disease. A large number of patients with large bone defects can onl...

Claims

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Application Information

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IPC IPC(8): A61L27/12A61L27/20A61L27/22A61L27/38A61L27/58A61L27/50B33Y10/00B33Y70/00
CPCA61L27/12A61L27/20A61L27/222A61L27/3808A61L27/3821A61L27/3834A61L27/3847A61L27/3886A61L27/50A61L27/58A61L2430/02B33Y10/00B33Y70/00C08L5/08C08L5/04
Inventor 胡庆夕李帅刘媛媛孙程艳沈显虎张毅汪羽连红军
Owner SHANGHAI UNIV
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