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BGIos116 gene and application thereof

A kind of use, technology of transgenic plants, applied in the field of genes, can solve the problems such as research on genes related to rice differentiation and emergence that have not been seen

Active Publication Date: 2012-06-20
深圳华大基因农业控股有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research on genes related to rice differentiation and emergence

Method used

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  • BGIos116 gene and application thereof
  • BGIos116 gene and application thereof
  • BGIos116 gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1: PCR amplification of BGIos116 gene and construction of pMD18-T+BGIos116 recombinant vector

[0057] PCR amplification

[0058]Genomic DNA (gDNA) of common wild rice Yuanjiang (Oryza. , respectively design a pair of PCR-specific amplification primers at the beginning and end (upstream primer F1, plus restriction site BamHI and protection base, downstream primer R1, plus restriction site Xba I and protection base). Using the gDNA extracted from Yuanjiang as a template, high-fidelity Ex Taq (TaKaRa, DRR100B) polymerase was used for PCR amplification. As shown in Table 1.

[0059] Table 1 PCR system for target gene amplification

[0060] Composition Volume (μl)

[0061] Genomic DNA 0.2

[0062] dNTPs (2.5mM) 2

[0063] 10×Ex Buffer (with magnesium ions) 2.5

[0064] Primer F1 (50μM) 1

[0065] Primer R1 (50μM) 1

[0066] Ex taq 0.2

[0067] wxya 2 O supplemented to a total volume of 25 μl

[0068] The PCR amplification program was as follows: 94°C p...

Embodiment 2

[0082] Embodiment 2: Construction of p6 recombinant vector

[0083] 1) PCR amplification of maize Ubiquitin promoter fragment and construction of pMD18-T+Ubi recombinant vector

[0084] Ubi promoter PCR amplification

[0085] Genomic DNA of maize variety B73 (Zea mays mays cv.B73) was extracted using a plant genomic DNA extraction kit (TIANGEN New Plant Genomic DNA Extraction Kit, catalog number: DP320-02) (http: / / www.maizegdb.org / ), according to the sequence of the promoter in maize B73gDNA, a pair of PCR-specific amplification primers were designed at the beginning and the end respectively (upstream primer F2: GG CTGCAG TGCAGCGTGACCCGGTCGT (SEQ ID NO: 4), plus restriction site Pst I and protection base, downstream primer R2: GG CTGCAG AAGTAACACCAAAC (SEQ ID NO: 5), plus restriction site Pst I and protection bases). Using the above extracted gDNA of corn B73 as a template, high-fidelity Ex Taq (TaKaRa, DRR100B) polymerase was used for PCR amplification. As shown in t...

Embodiment 3

[0146] Embodiment 3: Construction of p6+BGIos116 recombinant vector

[0147] Extract the pMD18-T+BGIos116 plasmid, and recover the BGIos116 gene fragment after digestion with the restriction endonuclease BamHI / XbaI, and extract the p6 plasmid at the same time, and recover the large fragment after digestion with the corresponding restriction endonuclease BamHI / XbaI. The product was ligated, and then the competent cell DH5α was transformed to obtain the recombinant vector p6+BGIos116. Positive clones were screened for PCR detection and then sequenced for confirmation.

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PUM

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Abstract

The invention relates to a BGIos116 gene and application thereof. The BGIos116 gene has a nucleotide sequence shown in SEQ ID NO:1, or has a variant with function of promoting plants to quickly differentiate and produce shoots. The invention also relates to a transgenic plant with the BGIos116 gene or the variant thereof. The invention also relates to the application of the BGIos116 gene and the variant thereof in promoting plants to quickly differentiate and produce shoots, and a method for promoting plants to quickly differentiate and produce shoots.

Description

technical field [0001] The invention relates to a gene, in particular to the BGIos116 gene, and its use for promoting rapid differentiation and emergence of plants, especially monocotyledonous plants. Background technique [0002] Rice is the most widely consumed and oldest crop in the world. Rice is the staple food of more than 2.5 billion people in the world. For nearly half a century, the progress of rice farming technology in the "Green Revolution" has promoted a substantial increase in grain production in Asia and the world, greatly improved the rice production capacity of major rice-producing countries in Asia, and contributed to the economic growth and social stability of these countries. Alleviating population growth plays an important role in the pressure on food, resources and environment. However, due to the limited germplasm resources of Oryza genus and the limitations of conventional breeding methods in time and space, the further genetic improvement of rice i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12N15/82C12N5/10A01H4/00
Inventor 杨爽孙红正李宁倪雪梅
Owner 深圳华大基因农业控股有限公司
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