Rice callus differentiation culture medium as well as preparation method and application thereof
A technology for differentiation medium and callus, which is applied to the field of rice callus differentiation medium and its preparation, can solve the problems of time-consuming, many methods, pollution, etc. The effect of good, easy differentiation conditions
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[0048] Example 1
[0049] A rice callus differentiation medium (abbreviated as MSK1N0.5) is prepared by adding KT, NAA and sorbitol to MS basic medium; wherein, the KT, NAA and sorbitol are in the rice The concentration of wound tissue differentiation medium is 1mg / L, 0.5mg / L and 40g / L; the rice callus differentiation medium also contains sucrose at a concentration of 30g / L and Phytagel plant at a concentration of 3g / L gel.
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[0050] Example 2
[0051] A rice callus differentiation medium (abbreviated as MSK1N1) is prepared by adding KT, NAA and sorbitol to MS basal medium; wherein, the KT, NAA and sorbitol are in the rice callus The concentrations in the differentiation medium are respectively 1 mg / L, 1 mg / L and 40 g / L; the rice callus differentiation medium also contains sucrose at a concentration of 30 g / L and Phytagel plant gel at a concentration of 3 g / L.
Example Embodiment
[0052] Example 3
[0053] A rice callus differentiation medium (abbreviated as MSK1N1.5) is prepared by adding KT, NAA and sorbitol to MS basal medium; wherein, the KT, NAA and sorbitol are in the rice The concentrations in the wound tissue differentiation medium were 1mg / L, 1.5mg / L and 40g / L; the rice callus differentiation medium also contained sucrose at a concentration of 30g / L and Phytagel plant at a concentration of 3g / L gel.
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