Method for acquiring regeneration seedlings of dove trees through tissue culture by taking dove tree leaves as explants

A tissue culture and explant technology, applied in plant regeneration, botanical equipment and methods, horticultural methods, etc., can solve problems such as low efficiency and destruction of endangered plants of Dove tree, achieving no seasonal restrictions, good overall efficiency, The effect of a large number of rooting strips

Active Publication Date: 2013-02-13
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the only research on the regeneration system, the winter buds or stems with buds of Davidia involucrata are mostly used as explants. During operation, it is necessary to collect very important buds or stems in the growth process of Davidia involucrata, which will cause damage to Davidia involucr

Method used

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  • Method for acquiring regeneration seedlings of dove trees through tissue culture by taking dove tree leaves as explants
  • Method for acquiring regeneration seedlings of dove trees through tissue culture by taking dove tree leaves as explants
  • Method for acquiring regeneration seedlings of dove trees through tissue culture by taking dove tree leaves as explants

Examples

Experimental program
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Effect test

Example Embodiment

[0036] Example 1

[0037] In this example, young Davidia involucrata leaves were collected from adult Davidia involucrata plants in Yinchanggou Scenic Area, Pengzhou City, Sichuan Province (see figure 1 ), the sterilization operation of the young Davidia involucrata leaves is as follows: the detergent (white cat brand) is made into a dilution with tap water, the volume ratio of the detergent to the tap water is 1:1000, and the collected young The leaves of Davidia involucrata were soaked in the detergent diluent for 10 minutes, then taken out and rinsed with tap water for 1 h to clean the surface debris, then sterilized with ethanol with a mass concentration of 75% under aseptic conditions for 30 seconds, and then put in a 0.1% mass concentration Sterilize in mercury chloride aqueous solution for 4 minutes, then take it out and rinse it with sterile water 5 times to complete the sterilization. Cut the sterilized Davidia involucrata leaves with scissors to a size of about 1 cm 2 T...

Example Embodiment

[0053] Example 2

[0054] In this example, young Davidia involucrata leaves were collected from adult Davidia involucrata plants in Emeishan Scenic Area, Emeishan City, Sichuan Province, and the sterilization operation of the young Davidia involucrata leaves was the same as in Example 1. Cut the sterilized Davidia involucrata leaves with scissors to a size of about 1 cm 2 The small pieces are used for inoculation.

[0055] In this embodiment, the steps of the method for obtaining regenerated seedlings of Davidia involucrata by tissue culture using Davidia involucrata leaves as explants are as follows:

[0056] (1) Obtained callus from leaves of Davidia involucrata

[0057] Use a 100ml Erlenmeyer flask, fill each bottle with about 40ml callus induction medium, inoculate 5~6 small pieces of sterilized Davidia involucrata leaves (with the back of the leaf facing up), and cultivate for 28 days in the dark to obtain recovery of Davidia involucrata leaves. Wound tissue, the callus inductio...

Example Embodiment

[0070] Example 3

[0071] In this example, young Davidia involucrata leaves were collected from adult Davidia involucrata plants in Longcanggou Nature Reserve, Yingjing County, Ya'an City, Sichuan Province, and the sterilization operation of the young Davidia involucrata leaves was the same as in Example 1. Cut the sterilized Davidia involucrata leaves with scissors to a size of about 1 cm 2 The small pieces are used for inoculation.

[0072] In this embodiment, the steps of the method for obtaining regenerated seedlings of Davidia involucrata by tissue culture using Davidia involucrata leaves as explants are as follows:

[0073] (1) Obtained callus from leaves of Davidia involucrata

[0074] Use a 100ml Erlenmeyer flask, fill each bottle with about 40ml callus induction medium, inoculate 5-6 small pieces of sterilized Davidia involucrata leaves (with the back of the leaf facing up), and cultivate for 30 days in the dark to obtain recovery of Davidia involucrata leaves. Wounded tissu...

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Abstract

The invention relates to a method for obtaining regeneration seedlings of dove trees through tissue culture by taking dove tree leaves as explants. The method comprises the following steps: step of: (1) acquiring calluses of the dove tree leaves; (2) breeding the calluses; (3) inducing cluster buds; (4) breeding the cluster buds; and (5) inducing roots. According to the method, the environmental temperature in steps (1)-(5) are 22-26 DEG C, and the environmental humidity is preferably 60%-90%. According to the method, the differentiation rate of the calluses is higher and reaches more than 50%; the rooting percentage is 100%; and the survival rate of transplanting is more than 90%.

Description

technical field [0001] The invention belongs to a method for obtaining regenerated seedlings through tissue culture, in particular to a method for obtaining regenerated seedlings of Davidia involucrata through tissue (leaf) culture. Background technique [0002] Davidia involucrata Baillon (Davidia involucrata Baillon), also known as water pear or pigeon tree, is a plant of the genus Davidia (Davidiaceae) in the family Davidiaceae. The relict species are listed as national first-level key protected plants and need to be strengthened for protection. The height of Davidia involucrata tree is 10-20 meters. When it blooms, the structure of its brown-red flower heads and two bracts at the base resembles white doves. It looks like a group of white doves inhabiting in the shade from a distance, so it also has a high Ornamental value. [0003] It is not easy to reproduce Davidia involucrata with seeds, and the germination rate is often low and the germination time is long, so asex...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 徐莺陈蕤坤陈放
Owner SICHUAN UNIV
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