Method for acquiring regeneration seedlings of dove trees through tissue culture by taking dove tree leaves as explants
A tissue culture and explant technology, applied in plant regeneration, botanical equipment and methods, horticultural methods, etc., can solve problems such as low efficiency and destruction of endangered plants of Dove tree, achieving no seasonal restrictions, good overall efficiency, The effect of a large number of rooting strips
Active Publication Date: 2013-02-13
SICHUAN UNIV
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Abstract
The invention relates to a method for obtaining regeneration seedlings of dove trees through tissue culture by taking dove tree leaves as explants. The method comprises the following steps: step of: (1) acquiring calluses of the dove tree leaves; (2) breeding the calluses; (3) inducing cluster buds; (4) breeding the cluster buds; and (5) inducing roots. According to the method, the environmental temperature in steps (1)-(5) are 22-26 DEG C, and the environmental humidity is preferably 60%-90%. According to the method, the differentiation rate of the calluses is higher and reaches more than 50%; the rooting percentage is 100%; and the survival rate of transplanting is more than 90%.
Application Domain
Plant tissue cultureHorticulture methods
Technology Topic
Cell buddingTransplanting +6
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