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Method for acquiring regeneration seedlings of dove trees through tissue culture by taking dove tree leaves as explants

A tissue culture and explant technology, applied in plant regeneration, botanical equipment and methods, horticultural methods, etc., can solve problems such as low efficiency and destruction of endangered plants of Dove tree, achieving no seasonal restrictions, good overall efficiency, The effect of a large number of rooting strips

Active Publication Date: 2013-02-13
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the only research on the regeneration system, the winter buds or stems with buds of Davidia involucrata are mostly used as explants. During operation, it is necessary to collect very important buds or stems in the growth process of Davidia involucrata, which will cause damage to Davidia involucrata. The destruction of this endangered plant
However, the efficiency of the current regeneration system using Davidia involucrata leaves as explants is extremely low, and the only successful report has a callus differentiation rate of only 6%, which cannot meet the needs of practical applications.

Method used

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  • Method for acquiring regeneration seedlings of dove trees through tissue culture by taking dove tree leaves as explants
  • Method for acquiring regeneration seedlings of dove trees through tissue culture by taking dove tree leaves as explants
  • Method for acquiring regeneration seedlings of dove trees through tissue culture by taking dove tree leaves as explants

Examples

Experimental program
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Effect test

Embodiment 1

[0037] In this embodiment, young and tender Davidia involucrata leaves are collected from the adult Davidia involucrata plant of Yinchanggou Scenic Area, Pengzhou City, Sichuan Province (see figure 1 ), the sterilization operation of the young Davidia involucrata leaves is as follows: the detergent (White Cat brand) is prepared into a dilution with tap water, and the volume ratio of the detergent to tap water is 1:1000, and the collected young Soak the leaves of Davidia involucrata in the detergent diluent for 10 minutes, take them out and rinse them with tap water for 1 hour to clean the surface debris, then disinfect them with ethanol with a mass concentration of 75% for 30 seconds under aseptic conditions, and then put in 0.1% ethanol Disinfect in mercuric chloride aqueous solution for 4 min, then take it out and rinse it with sterile water for 5 times to complete the sterilization. Cut the sterilized leaves of Davidia involucrata into about 1 cm in size with scissors 2 Sm...

Embodiment 2

[0054] In this embodiment, young leaves of Davidia involucrata are collected from adult Davidia involucrata plants in Mount Emei Scenic Area, Emeishan City, Sichuan Province, and the sterilization operation of the young leaves of Davidia involucrata is the same as that in Example 1. Cut the sterilized leaves of Davidia involucrata into about 1 cm in size with scissors 2 Small pieces are used for inoculation.

[0055] In the present embodiment, the method steps of obtaining the regenerated seedlings of Davidia involucrata through tissue culture using the leaves of Davidia involucrata as explants are as follows:

[0056] (1) Obtained callus from leaves of Davidia involucrata

[0057] Use a 100ml Erlenmeyer flask, fill each bottle with about 40ml of callus induction medium, inoculate 5~6 small pieces of sterilized leaves of Davidia involucrata (the back of the leaves face up), and culture in the dark for 28 days, and the leaves of Davidia involucrata are healed Wounded tissue, ...

Embodiment 3

[0071] In this example, young Davidia involucrata leaves were collected from adult Davidia involucrata plants in Longcanggou Nature Reserve, Yingjing County, Ya'an City, Sichuan Province, and the sterilization operation of the young Davidia involucrata leaves was the same as in Example 1. Cut the sterilized leaves of Davidia involucrata into about 1 cm in size with scissors 2 Small pieces are used for inoculation.

[0072] In the present embodiment, the method steps of obtaining the regenerated seedlings of Davidia involucrata through tissue culture using the leaves of Davidia involucrata as explants are as follows:

[0073] (1) Obtained callus from leaves of Davidia involucrata

[0074] Use a 100ml Erlenmeyer flask, fill each bottle with about 40ml of callus induction medium, inoculate 5~6 small pieces of sterilized leaves of Davidia involucrata (the back of the leaves face up), and culture in the dark for 30 days, and the leaves of Davidia involucrata are healed injured ti...

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Abstract

The invention relates to a method for obtaining regeneration seedlings of dove trees through tissue culture by taking dove tree leaves as explants. The method comprises the following steps: step of: (1) acquiring calluses of the dove tree leaves; (2) breeding the calluses; (3) inducing cluster buds; (4) breeding the cluster buds; and (5) inducing roots. According to the method, the environmental temperature in steps (1)-(5) are 22-26 DEG C, and the environmental humidity is preferably 60%-90%. According to the method, the differentiation rate of the calluses is higher and reaches more than 50%; the rooting percentage is 100%; and the survival rate of transplanting is more than 90%.

Description

technical field [0001] The invention belongs to a method for obtaining regenerated seedlings through tissue culture, in particular to a method for obtaining regenerated seedlings of Davidia involucrata through tissue (leaf) culture. Background technique [0002] Davidia involucrata Baillon (Davidia involucrata Baillon), also known as water pear or pigeon tree, is a plant of the genus Davidia (Davidiaceae) in the family Davidiaceae. The relict species are listed as national first-level key protected plants and need to be strengthened for protection. The height of Davidia involucrata tree is 10-20 meters. When it blooms, the structure of its brown-red flower heads and two bracts at the base resembles white doves. It looks like a group of white doves inhabiting in the shade from a distance, so it also has a high Ornamental value. [0003] It is not easy to reproduce Davidia involucrata with seeds, and the germination rate is often low and the germination time is long, so asex...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 徐莺陈蕤坤陈放
Owner SICHUAN UNIV
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