55results about How to "Not limited by season" patented technology

The invention provides a negative-pressure liquid nitrogen subcooler and a method therefore for reducing liquid nitrogen temperature. The negative-pressure liquid nitrogen subcooler comprises a liquid nitrogen conveying system, a subcooler system, a negative-pressure air exhausting system and a discharging system, wherein the liquid nitrogen conveying system is used for supplying liquid nitrogen to the subcooler system; the negative-pressure air exhausting system is used for reducing pressure for the subcooler; and the discharging system is used for discharging gasified nitrogen. In the negative-pressure liquid nitrogen subcooler, an electric heater is adopted to raise the temperature of gaseous nitrogen to 20-30 DEG C, so that the subcooler is not limited to the weathers; a liquid level sensor is adopted to automatically regulating the opening of a pneumatic regulating valve, so that the height of the liquid level is maintained. The method comprises the steps of: firstly, injecting the liquid nitrogen for a liquid nitrogen container and regulating pressure; then injecting the liquid nitrogen for the subcooler, wherein the height of the liquid level of the liquid nitrogen in the subcooler is kept constant; then starting the negative-pressure air exhausting system, and cooling the liquid nitrogen through a heat exchanger; and finally, recovering the liquid nitrogen and discharging the liquid nitrogen. According to the invention, the temperature of a heat sink can be further reduced, the temperature of the heat sink is regulatable within a range of 70-77k, and the advantagesof simple equipment, good effect and low operating cost are achieved.

The invention belongs to the technical field of old vinegar brewing and particularly relates to a vinegar and a preparation method thereof. The method includes raw material preprocessing, smashing, moisturizing, pasting, liquefying, liquid stationary alcoholic fermentation, stirring, vat feeding, solid acetic acid fermentation, smoking, vinegar pouring and ageing. Meanwhile, a fulled-sealed device is adopted for automatic stirring, producing and fermenting during alcohol fermentation, a traditional vat is adopted during acetic acid fermentation, fermentation is implemented through manual turning, and the finished vinegar can be obtained. The method has the advantages of strain excellence and process reasonableness, the fermentation period is shortened, the raw material utilization rate and vinegar finishing rate are increased, season limitation is eliminated, and the advantages of sour, sweet, soft and sweet tastes of the traditional vinegar are maintained.

The invention discloses a compound microorganism bacterium agent for treating poultry excrement to prepare composts and a preparation method of the compound microorganism bacterium agent. The compound microorganism bacterium agent is prepared from bacillus megaterium, Saccharomyces cerevisiae, bacillus licheniformis, streptomyces albogriseolus and lactobacillus plantarum. The preparation method comprises the following steps of: A. cultivation of the bacillus megaterium and the bacillus licheniformis, to be specific, inoculating the bacillus megaterium and the bacillus licheniformis to a beef extract peptone solid medium for cultivation; B. cultivation of the Saccharomyces cerevisiae, to be specific, inoculating the Saccharomyces cerevisiae to a lactic acid-potato-glucose solid medium for cultivation; C. cultivation of the lactobacillus plantarum, to be specific, inoculating the lactobacillus plantarum on an MRS (De Man, Rogosa and Sharpe) medium to a solid medium for cultivation; D. cultivation of the streptomyces albogriseolus, to be specific, inoculating the streptomyces albogriseolus to a solid medium for cultivation; and E. stirring to mix the cultivated bacterium agent, and ventilating and drying the bacterium agent at a low temperature to obtain the compound microorganism preparation. The compound microorganism bacterium agent for treating the poultry excrement to prepare the composts and the preparation method of the compound microorganism bacterium agent disclosed by the invention have the advantages of reasonable formula, capability of quickly removing smell of the poultry excrement during the treatment and producing organic fertilizers through decomposed conversion, easy implementation as well as simplicity and convenience for operation.

The invention discloses a steel plate concrete column and a construction method, and the method is characterized in that a plastic skin aluminum core lead wire is built in on a steel bar before concrete is poured, unhardened concrete is heated by utilizing an electromagnetic inductive method, and when the concrete is hardened, the shrinkage of a steel plate is greater than that of the concrete, i.e., prestressing force is applied to a steel tube by the concrete. Before the concrete is poured, an inbuilt conducting tube is connected with an anode power supply, the steel bar is connected with a negative power source, the power source is switched on in the process of pouring the concrete, and free water in the concrete is discharged through different electric potential distributions formed in the concrete. The steel plate concrete column provided by the invetnion has the advantages of high intensity, light weight, good plasticity, shock resistance, fatigue resistance, and the like; and the construction method provided by the invention has the characteristics that templates are omitted, labor and materials are saved, the construction is convenient, no season restriction exists, and the like.

The invention relates to a sweet potato drought tolerance identification method, and in particular relates to an indoor auxiliary sweet potato drought tolerance identification method, belonging to the technical field of sweet potato drought tolerance identification. The method comprises the following steps: by selecting a test tube seedling stem section which is subjected to subculture for more than or just 50 days as an explant, cutting a single section, inoculating the explant into a high-permeation identification culture medium, performing inoculation treatment for 30 days, subsequently surveying the relative root growth number coefficients of different varieties, and judging the drought tolerance grade of the varieties according to the relative root growth number coefficients of different varieties, wherein the drought tolerance of the variety of which the relative root growth number coefficient is greater than or equal to 1.0 is high, the drought tolerance of the variety of which the relative root growth number coefficient is greater than 0.5 and less than 1.0 is medium, and the drought tolerance of the variety of which the relative root growth number coefficient is smaller than or equal to 0.5 is relatively poor. The method is simple to operate, is flexible and convenient and applicable to screening on a great number of materials, can relieve the workload of field drought tolerance identification and can improve the working efficiency.

The invention relates to a sand-protecting barrier prepared from biodegradable materials, comprising a biodegradable polylactic acid fiber (PLA) textile, wherein the polylactic acid fiber (PLA) textile is made to a drum-shaped flush system sand-protecting barrier and a drum-shaped low vertical type sand-protecting barrier, diameters of the flush system sand-protecting barrier and the low vertical type sand-protecting barrier are 8-25 cm, and the height of the drum-shaped low vertical type sand-protecting barrier is 15-35 cm. A preparation method of the sand-protecting barrier comprises a flush system preparation method and a low vertical type preparation method, wherein the flush system preparation method and the low vertical type preparation method both comprise a grid type preparation method and a row type preparation method. The flush system sand-protecting barrier and the low vertical type sand-protecting barrier which are prepared by polylactic acid fibers (PLA) overcome secondary pollution and have low cost. The grid type preparation method and the row type preparation method adopted by the flush system sand-protecting barrier and the low vertical type sand-protecting barrier ensure the effective protection of the sandy soil of protected areas, prevent the sandy soil loss, are simple and have obvious effect.

The tissue culture breeding process for clonic fibraurea stem includes the following steps: collecting flush explant of height 15-40 cm without wound and cracking; sterilizing the explant through first washing with washing powder and then sterilization with mercuric chloride in 0.1 % concentration for 1-2 min and thrice rinsing with bacteria-free water; soaking the explant with solution of cysteine or ascorbic acid to prevent browning; bushy bud introduction and secondary proliferation; and rooting culture with preferably seedling of 2 cm height. The said process with flush explant can propagate excellent seedling of clonic line and the present invention has wide application foreground.

The invention relates to a method for preparing coniferous polypentenol by adopting the solvent extraction method and the molecular distillation method; fresh pine branch tender leaves are dried and smashed into pine needle powder with 16 to 30 meshes as a raw material, petroleum ether is used for extraction, then the freezing dewaxing is carried out at the temperature of 0 to 25 DEG C, and a polyprenol crude product is prepared by concentration, saponification and extraction; the polyprenol crude product is separated and purified through a first-stage molecular distillation device and a second-stage molecular distillation device, thereby preparing a high-purity polyprenol finished product. The raw material is sufficient, the solvent extraction method and the molecular distillation method are projects with high efficiency, energy conservation and environmental protection; the prepared product has no solvent residue, and the purity thereof is high, thereby being in line with the modern requirements of green and environmental protection; and the method can not only improve the purity and the yield of the polyprenol, but also can be in line with the industrial production requirements, improve the traditional process and realize the clean production. The polyprenol product produced by the technology has higher purity and can be widely used in health care foods, nutrition supplements and bio-pharmaceutical industry.

The invention discloses a method for quickly and accurately detecting the purity of a hybrid rice seed, which comprises the following steps: according to seed purity requirements, calculating the quantity of samples; sampling in a quantity-equal manner and mixing extracted DNA (deoxyribonucleic acid), or respectively extracting DNA and then mixing in a quantity-equal manner; designing PCR (polymerase chain reaction) primers according to the base sequence of detected sites, and amplifying the detected sites; recovering and purifying the PCR amplification products; building a library by using the amplification products, and performing high-flux sequencing; and calculating the seed purity according to the sequence proportion of the detected sites. The invention has the obvious characteristic that the requirements for detection speed and accuracy in the purity detection of a commodity hybrid seed are well satisfied.

The invention discloses a method for synthesizing arbutin by utilizing hairy roots of scutellaria baicalensis to convert hydroquinone. In the method, the arbutin is synthesized by cultivating the hairy roots of scutellaria baicalensis and converting hydroquinone by cells of the hairy roots of scutellaria baicalensis. The content of the arbutin in the converted hairy roots of scutellaria baicalensis can reach 4 to 6 percent. The conversion rate of the hydroquinone can reach 50 to 60 percent. The method has the characteristics of low pollution, few by-products and the like. A novel technology and a novel approach are provided for the production of the arbutin.

The invention relates to a mulberry silkworm feeding machine which comprises a discharge device, an unloading device, a conveying device and a rack. The discharge device comprises a storage bin, a spiral conveying pipe, a second motor, a hose, a funnel, a shell and a support frame; the unloading device comprises a third motor, a guide rail base, a synchronous belt, a guide rail, a slider, an unloading pipe, a fixing plate and a tank chain; the conveying device comprises a first motor, a conveyor belt, a drum, a belt pulley and a V belt; feed can be added into the storage bin, silkworm rearingtrays with to-be-fed mulberry silkworms can be arranged on the conveyor belt, the spiral conveying pipe can be driven under the rotation actions of the second motor to rotate, and accordingly the feedcan be conveyed and unloaded; the unloading pipe can be driven by the slider to move in the front-back directions, so that feed releasing work can be carried out on each silkworm rearing tray. The mulberry silkworm feeding machine has the advantages that the rotational speeds of the three motors can be set and adjusted, and accordingly the conveying speed of the conveyor belt, the feeding speed of the spiral conveying pipe, the left-right movement range of the slider and the left-right movement speed of the slider can be adjusted; the problems of deficiency of special young silkworm combinedrearing equipment, inaccuracy in feeding capacity control and heavy and monotonous work at present can be effectively solved by the aid of the mulberry silkworm feeding machine.

The present invention relates to a seedling method for cyclocarya paliurus seeds. The method is characterized in comprising the following steps: a. seed selecting; b. seed treating; c. seed germination accelerating; and d. seed seedling. According to the method provided by the present invention, the seedlings emerge in 30-60 days after sowing of the cyclocarya paliurus seeds, the emergence rate is over 90%, and the seedling survival rate is over 95%.

The invention belongs to the field of biotechnology. The invention discloses a method for identifying soybean resources resistant to race 4 of soybean cyst nematode and a special SSR primer. The SSR marker for identifying resistance to race 4 of soybean cyst nematode provided by the invention is composed of Satt533 and Satt504. The method for identifying soybean resistance and susceptibility includes such step as carrying out experiments on soybean resistance and susceptibility resources from home and abroad, carrying out identification on that soybean resistance and susceptibility resources,carrying out identification on the soybean resistance and susceptibility resources, carrying out identification on the soybean resistance and susceptibility resources, and carrying out identificationon the soybean resistance and susceptibility resources. Compared with the identification method of diseased soil, the method has the advantages of saving time and labor, not limited by seasons, and has a better application prospect. It is better to serve the identification and evaluation of soybean cyst nematode race 4 in production practice. As such, that SSR mark combination and the method for identifying soybean cyst nematode resistance race 4 have wide application prospect in soybean cyst nematode resistance race 4 resource screening, variety breeding, and the like.

The invention discloses a special primer combination for quickly detecting polyploid of a willow tree and a detection method for the polyploidy. A primer provided by the invention is used for performing PCR amplification on a DNA template of the to-be-detected willow tree; by means of electrophoresis detection of an amplification product, if the number of alleles detected on amplification sites of any pair of primers of the detected sample is greater than 2, the sample is the polyploid. Compared with the existing willow tree polyploid detection method, the special primer combination which is provided by the invention and is used for willow tree polyploid screening has the advantages of simple and quick operation method and reliable result, can detect a large quantity of samples within short time, and supplies efficient and reliable technical measures to willow tree polyploid screening. An important technical support is supplied to willow tree polyploid cultivation; the special primer combination has extremely high actual application values, can achieve relatively high economic and social benefits, and has wide application prospects.

The invention provides a suspension-cultured cell of Acer ginnala Maxim for producing gallic acid, which is characterized in that a proper amount of callus of Acer ginnala Maxim is inoculated in a solid cultivation medium containing WPM+TDZ 0.002-0.01 mg / L+6-BA 0.05-0.15 mg / L for successive transfer culture for 15 to 20 days to obtain a first successive generation; a well-growing callus is transformed into a triple WPM liquid medium containing a large amount of elements and having a pH value of 5.8 to 6.0, in the presence of a hormone including TDZ 0.002 to 0.010 mg / L and 6-BA 0.05 to 0.15 mg / L and 2% to 3% sugar to obtain the fresh weight of an inoculum size of 20 to 40 g / L; and the culture conditions comprises the temperature of 23 to 25 DEG C, the rotation speed of 110 to 130 rpm, the illumination time of 8 to 10 h / d, the illumination intensity of 1,400 to 1,700 Lx, and the culture period of the first successive generation of 7 to 15 days. The cells are collected after one period of 20 to 30 days, with the fresh weight up to 478.2 g / L and the dry weight up to 22.3 g / L, wherein the content of gallic acid is up to 2.29%, the output of gallic acid is 0.51g / L, and the gallic acid with the amount of 6.1227 g / L can be collected in one year. The invention can improve the output of suspension of gallic acid in Acer ginnala Maxim by cell engineering method for the first time, which solves the raw material problem for the production of gallic acid. The invention has the advantages of easy operation, short cycle, low cost as well as greatly saved labor force and material resource,improved labor productivity and protection of environment and Acer ginnala Maxim resources.

The invention discloses a ficus carica living body bleaching sap minimal invasive collecting device. The device comprises a liquid suction needle head, a negative pressure liquid collecting device andan air suction device which are sequentially connected, the liquid suction needle head, the negative pressure liquid collecting device and the air suction device are connected through hoses; the negative pressure liquid collecting device comprises a negative pressure liquid collecting cavity and a liquid collecting bottle, the part between the negative pressure liquid collecting cavity and the liquid collecting bottle is through, and the liquid collecting liquid is detachable. According to the collecting device, the swelling of ficus carica fruit root systems is utilized to directly performing minimal invasive operation on a ficus carica plant to collect the bleaching sap which contains higher concentration of ficus carica protease, and the activity of the ficus carica protease is preserved to the maximum extent.

The invention relates to a sand-protecting barrier prepared from biodegradable materials, comprising a biodegradable polylactic acid fiber (PLA) textile, wherein the polylactic acid fiber (PLA) textile is made to a drum-shaped flush system sand-protecting barrier and a drum-shaped low vertical type sand-protecting barrier, diameters of the flush system sand-protecting barrier and the low verticaltype sand-protecting barrier are 8-25 cm, and the height of the drum-shaped low vertical type sand-protecting barrier is 15-35 cm. A preparation method of the sand-protecting barrier comprises a flush system preparation method and a low vertical type preparation method, wherein the flush system preparation method and the low vertical type preparation method both comprise a grid type preparation method and a row type preparation method. The flush system sand-protecting barrier and the low vertical type sand-protecting barrier which are prepared by polylactic acid fibers (PLA) overcome secondary pollution and have low cost. The grid type preparation method and the row type preparation method adopted by the flush system sand-protecting barrier and the low vertical type sand-protecting barrier ensure the effective protection of the sandy soil of protected areas, prevent the sandy soil loss, are simple and have obvious effect.

The invention discloses a soilless seedling culturing method of arrowheads and belongs to the technical field of agriculture, in particular vegetable cultivation. The soilless seedling culturing method includes cutting off terminal buds free of tuber at the joint of the terminal buds and the seedball before winter of the first year, soaking the terminal buds in saturated bleaching powder after cleaning, washing by tap water and refrigerating after drying; vertically inserting the terminal buds into a matrix soaked with water in the second year, disposing a seedling cultivating tray at 20-25 degrees Centigrade for 16 hours of light period, cultivating under the condition of 2000-3000 lux of light intensity and transplanting the seedlings to the field until the arrowhead seedlings grow with 2 to 3 leaves. The seedlings are cultivated under the controllable cultivating condition, and the integral seedling cultivation time is shorter than 25 days; on one hand, labor can be saved, and management is facilitated; on the other hand, season limits are broken through, and technical basis is provided for industrial cultivation and year-round cultivation of the arrowheads.

The invention discloses a preparation method of vacuum-packaged boiled pleurotus eryngii. The preparation method comprises the steps of raw material checking and accepting, finishing, cleaning, arrangement, processing, preboiling, cooling, leaching water, examination, bagging, adding soup, sealing, carrying out sterilization, cooling, drying and wiping bag, wherein the soup adding step particularly comprises the step of after the bagging step, adding clean water into a bag as a soup base according to the proportion that solids take 25-35% of the materials in weight after the soup is added; the sterilization and the cooling steps particularly comprises the steps of carrying out automatic sterilization and cooling by virtue of a high-pressure and high-temperature sterilizing pot, wherein the sterilizing temperature is 121-123 DEG C, the time is 35-45 minutes, and the pressure is 0.2-0.23MPa. The preparation method has the advantages that the freshness of materials can be adequately maintained, meanwhile, the nutritional ingredients are not damaged, and the eating period of a product can be prolonged.

The invention relates to a rhizome-based Polygonatum cyrtonema Hua seedling culture method, and relates to a traditional Chinese medicinal material artificial reproduction technology. The method is characterized in that a TDZ solution or a 6KT solution is adopted to carry out soaking treatment on rhizome culture seedlings or new rhizome seedlings, the emergence rate of the obtained treated seedlings after transplanting reaches up to 58%, and the method is suitable for Polygonatum cyrtonema Hua industrialized seedling culture, the seedling emergence time is brought forward from February-March of the next year to November of the same year or even earlier, and therefore the seedling growth time is prolonged.

The invention discloses a method for cultivating bitter weed seedlings, which comprises the following steps: digging the seedbed to a depth of 8 to 10 centimeters, breaking the soil blocks, adding 0.3 to 0.5 kilograms of humus per square meter of the seedbed, stirring evenly, and then feeding the seedlings Add water to the field, and add bleaching powder to the water for disinfection; choose bitter grass with a plant height of 15 to 20 cm as seedlings, put them in the disinfection pool and soak them for 6 to 10 minutes; The seedlings are one-pot, spray the hormone combination solution on the seedbed, and then cover the seedlings with a film; control the water temperature in the nursery field to be 15 to 30 degrees, and when the seedlings are transplanted into the nursery field for 48 to 52 days, the new growth formed by the rhizomes will form. The bitter grass seedlings will grow to a plant height of 10 cm; remove the film covering the bitter grass seedlings, add water to the seedbed, keep it for 9 to 11 days, and move the seedlings out of the nursery field to obtain the bitter grass seedlings. The method of the invention breaks the season limitation of Erytheria galerica planting, and overcomes the disadvantages of high cost, long period and high operation requirements in its tissue culture technology.

The invention relates to a bud stock grafting method of Australian nuts. The grafting method comprises the following steps of: using Australian nuts for dibbled planting and culturing seedling sproutsof the nuts as bud stocks, using one-year-old branches which are lignified on the seed tree of Australian nuts as scion and grafting the branches on the bud stocks, temporarily planting the bud stocks in nutritional bags, planting the obtained nutritional bags with bud stocks on a seedling bed with shade net sheds and mulching film convex sheds and controlling the temperature and relative humidity in the sheds. The bud stock grafting method fully exerts the characteristics of strong regeneration capacity and vigorous growth of young tissues of the bud stocks, and effectively overcomes the defects that the forming layers of the young seedling stocks of the nuts are thin and the growth and coalescence are harder with the scions and grafting survival rate is low, thus greatly improving the grafting survival rate of the Australian nuts by more than 20 percent, shortening the period of out of nursery by more than one year, reducing the culturing cost of seedlings and increasing the economic benefit.

The invention discloses blood tofu, belongs to the technical field of food processing used for preparation of blood tofu and aims to solve the problem that preparation of existing blood tofu requiressmoking but the smoking is harmful to human health. The blood tofu of the invention is prepared from, by weight, 60-80 parts of bean curd, 8-12 parts of minced pork, 4-6 parts of fresh pig blood, 3-5parts of edible salt, 0.8-1.2 parts of Chinese prickly ash powder, 2-6 parts of peanut powder, 1-3 parts of sesame, 4-6 parts of bamboo shoot pulp, 0.4-0.6 part of dried tangerine or orange peel, 0.4-0.6 part of dried ginger, 1-3 parts of white granulated sugar, 1-6 parts of honey, and 2-8 parts of cooking wine. By quickly dehydrating blood tofu at high temperature firstly and then baking at low-temperature, the cooking wine can react with the white granulated sugar and the honey to generate aromatic ester substances, so that the blood tofu has fragrance; in addition, the white sugar and the honey can be colored in the baking process to endow the blood tofu with good sense; and smoking is not needed in the whole preparation process, so that the preparation method is environmentally friendly, and the problem that a large number of harmful ingredients are generated in the traditional smoking process is completely eradicated.

The invention discloses a method for cultivating vallisneria natans seedlings. The method includes steps of digging a seedling bed with the depth ranging from 8 centimeters to 10 centimeters, smashing soil blocks, adding from 0.3 to 0.5 kilogram of humus soil to each square meter of seeding bed, uniformly stirring the humus soil in the seedling bed, adding water into a seedling culture field and adding bleaching powder into the water for disinfection; selecting vallisneria natans with plant heights ranging from 15 centimeters to 20 centimeters as annual seedlings, and placing and soaking the annual seedlings in a disinfection tank for 6 to 10 minutes; transplanting the annual seedlings on the seedling bed, dividing each three to five annual seedlings as a stump, spraying combination hormone liquid on the seedling bed and covering a thin film on the annular seedlings; controlling the temperature of the water of the seedling culture field to range from 15 DEG C to 30 DEG C; and removing the thin film covering the vallisneria natans seedlings, filling water into the seedling bed, keeping the seedlings in the seedling bed for 9 to 11 days and shifting the seedlings out of the seedling culture field to obtain the vallisneria natans seedlings. After the annual seedlings are transplanted in the seedling culture field for 48 to 52 days, plant heights of new vallisneria natans seedlings grown on underground stems can reach 10 centimeters. The method has the advantages that seasonal limitation on plantation of the vallisneria natans can be broken through, and shortcomings of high cost, long period, high operational requirements and the like of a tissue culture technology for the vallisneria natans can be overcome.

The invention discloses a method for transplanting germinated tamarisk seedlings in saline-alkali land by using salt water. The method is to excavate tamarix seedlings, retain thick roots, trim off all germinated branches and leaves on the top of the seedlings and the trunk to obtain the trunk, and align the roots of the seedlings in the same direction. , binding; soak the bundled seedlings in salt water at the planting point for 24-72 h; dig tree pits, sprinkle diamine phosphate at the bottom of each tree pit, and plant a soaked sapling in each tree pit; plant Then pour the salt water at the planting point; cover the entire tree pit surface with plastic film, compact the plastic film on the edge of the tree pit with soil, and perform secondary watering according to the soil moisture content. The method provided by the invention can not only break the seasonal restrictions on the transplanting of tamarix seedlings, but also significantly reduce the consumption of fresh water, reduce the cost of greening saline-alkali land, and can significantly improve the transplanting survival rate of tamarix seedlings, which is beneficial to the original soil vegetation of coastal saline-alkali land. Construction is important.

The invention discloses a soilless seedling culturing method of arrowheads and belongs to the technical field of agriculture, in particular vegetable cultivation. The soilless seedling culturing method includes cutting off terminal buds free of tuber at the joint of the terminal buds and the seedball before winter of the first year, soaking the terminal buds in saturated bleaching powder after cleaning, washing by tap water and refrigerating after drying; vertically inserting the terminal buds into a matrix soaked with water in the second year, disposing a seedling cultivating tray at 20-25 degrees Centigrade for 16 hours of light period, cultivating under the condition of 2000-3000 lux of light intensity and transplanting the seedlings to the field until the arrowhead seedlings grow with 2 to 3 leaves. The seedlings are cultivated under the controllable cultivating condition, and the integral seedling cultivation time is shorter than 25 days; on one hand, labor can be saved, and management is facilitated; on the other hand, season limits are broken through, and technical basis is provided for industrial cultivation and year-round cultivation of the arrowheads.

The invention discloses a rapid propagation method for dendrobium officinale stem tissue culture, and belongs to the technical field of biology. According to the rapid propagation method, stem sections (above the third section from the root) of dendrobium officinale with the growth period more than one year are used for carrying out rapid propagation for the tissue culture. The rapid propagation method comprises the following steps of: preparation of implants except the stems, sterilization, induction culture, subculture, strong seedling culture, rooting culture and light culture hardening-seedling. The rapid propagation method for the dendrobium officinale stem tissue culture, provided by the invention, has the advantages of easiness of material obtaining, large material amount and no limitation on the seasons; the maternal biological property can be kept well; a culture technology is simple and easy to operate and according to the method, the low pollution rate and the rapid propagation speed can be realized; and the rapid propagation method for the dendrobium officinale stem tissue culture has the important meanings on resource regeneration and continuous use of scarce medicinal plants in imminent danger.

The invention belongs to the field of biotechnology and discloses a method for identifying soybean cyst nematode-resistant No. 4 race soybean resources and special SSR primers. The SSR markers identified against race 4 of soybean cyst nematode provided by the present invention consist of Satt533 and Satt504. The invention is verified through experiments on resistant and susceptible soybean resources from home and abroad, and the identification method of the invention is simple to operate, economical and efficient, and the result is stable and reliable. Compared with the diseased soil identification method, it has the advantages of saving time and effort, and is not limited by seasons. It has a better application prospect and better serves the identification and evaluation of soybean cyst nematode resistant race 4 in production practice. Therefore, the combination of SSR markers and the identification method for soybean cyst nematode-resistant race 4 of the present invention will have broad application prospects in the screening of soybean cyst nematode-resistant race 4 resources and variety breeding.

The invention discloses a brewing method of lotus-flavor liquor. The brewing method comprises the following steps: with wheat and lotuses as raw materials, carrying out natural bacterium culture, fermentation, drying and storage to obtain lotus koji; with sorghum, wheat, lotus leaves and lotus seeds as raw materials and the lotus koji as a saccharification leavening agent, successively conductinggrain moistening, grain steaming, cooling and koji mixing, fermentation, and distillation so as to obtain the lotus-flavor liquor. The liquor brewed by using the method has the fresh scent of the lotuses and mellow bouquet; effective components in both medicinal and edible raw materials (the lotuses, the lotus leaves and the lotus seeds) have the health preserving effects of clearing away heat andtoxic materials, tonifying the spleen and nourishing the stomach and the like; and compared with other pure wheat koji or rice koji, the lotus koji is applied to the saccharification process of the lotus seeds, so esterification effect is better, bouquet is mellower, and the utilization rate of the effective components in the lotus seeds is higher.