55results about How to "Not limited by season" patented technology

The invention provides a negative-pressure liquid nitrogen subcooler and a method therefore for reducing liquid nitrogen temperature. The negative-pressure liquid nitrogen subcooler comprises a liquid nitrogen conveying system, a subcooler system, a negative-pressure air exhausting system and a discharging system, wherein the liquid nitrogen conveying system is used for supplying liquid nitrogen to the subcooler system; the negative-pressure air exhausting system is used for reducing pressure for the subcooler; and the discharging system is used for discharging gasified nitrogen. In the negative-pressure liquid nitrogen subcooler, an electric heater is adopted to raise the temperature of gaseous nitrogen to 20-30 DEG C, so that the subcooler is not limited to the weathers; a liquid level sensor is adopted to automatically regulating the opening of a pneumatic regulating valve, so that the height of the liquid level is maintained. The method comprises the steps of: firstly, injecting the liquid nitrogen for a liquid nitrogen container and regulating pressure; then injecting the liquid nitrogen for the subcooler, wherein the height of the liquid level of the liquid nitrogen in the subcooler is kept constant; then starting the negative-pressure air exhausting system, and cooling the liquid nitrogen through a heat exchanger; and finally, recovering the liquid nitrogen and discharging the liquid nitrogen. According to the invention, the temperature of a heat sink can be further reduced, the temperature of the heat sink is regulatable within a range of 70-77k, and the advantagesof simple equipment, good effect and low operating cost are achieved.

The invention discloses a compound microorganism bacterium agent for treating poultry excrement to prepare composts and a preparation method of the compound microorganism bacterium agent. The compound microorganism bacterium agent is prepared from bacillus megaterium, Saccharomyces cerevisiae, bacillus licheniformis, streptomyces albogriseolus and lactobacillus plantarum. The preparation method comprises the following steps of: A. cultivation of the bacillus megaterium and the bacillus licheniformis, to be specific, inoculating the bacillus megaterium and the bacillus licheniformis to a beef extract peptone solid medium for cultivation; B. cultivation of the Saccharomyces cerevisiae, to be specific, inoculating the Saccharomyces cerevisiae to a lactic acid-potato-glucose solid medium for cultivation; C. cultivation of the lactobacillus plantarum, to be specific, inoculating the lactobacillus plantarum on an MRS (De Man, Rogosa and Sharpe) medium to a solid medium for cultivation; D. cultivation of the streptomyces albogriseolus, to be specific, inoculating the streptomyces albogriseolus to a solid medium for cultivation; and E. stirring to mix the cultivated bacterium agent, and ventilating and drying the bacterium agent at a low temperature to obtain the compound microorganism preparation. The compound microorganism bacterium agent for treating the poultry excrement to prepare the composts and the preparation method of the compound microorganism bacterium agent disclosed by the invention have the advantages of reasonable formula, capability of quickly removing smell of the poultry excrement during the treatment and producing organic fertilizers through decomposed conversion, easy implementation as well as simplicity and convenience for operation.

The invention discloses a steel plate concrete column and a construction method, and the method is characterized in that a plastic skin aluminum core lead wire is built in on a steel bar before concrete is poured, unhardened concrete is heated by utilizing an electromagnetic inductive method, and when the concrete is hardened, the shrinkage of a steel plate is greater than that of the concrete, i.e., prestressing force is applied to a steel tube by the concrete. Before the concrete is poured, an inbuilt conducting tube is connected with an anode power supply, the steel bar is connected with a negative power source, the power source is switched on in the process of pouring the concrete, and free water in the concrete is discharged through different electric potential distributions formed in the concrete. The steel plate concrete column provided by the invetnion has the advantages of high intensity, light weight, good plasticity, shock resistance, fatigue resistance, and the like; and the construction method provided by the invention has the characteristics that templates are omitted, labor and materials are saved, the construction is convenient, no season restriction exists, and the like.

The invention relates to a sweet potato drought tolerance identification method, and in particular relates to an indoor auxiliary sweet potato drought tolerance identification method, belonging to the technical field of sweet potato drought tolerance identification. The method comprises the following steps: by selecting a test tube seedling stem section which is subjected to subculture for more than or just 50 days as an explant, cutting a single section, inoculating the explant into a high-permeation identification culture medium, performing inoculation treatment for 30 days, subsequently surveying the relative root growth number coefficients of different varieties, and judging the drought tolerance grade of the varieties according to the relative root growth number coefficients of different varieties, wherein the drought tolerance of the variety of which the relative root growth number coefficient is greater than or equal to 1.0 is high, the drought tolerance of the variety of which the relative root growth number coefficient is greater than 0.5 and less than 1.0 is medium, and the drought tolerance of the variety of which the relative root growth number coefficient is smaller than or equal to 0.5 is relatively poor. The method is simple to operate, is flexible and convenient and applicable to screening on a great number of materials, can relieve the workload of field drought tolerance identification and can improve the working efficiency.

The tissue culture breeding process for clonic fibraurea stem includes the following steps: collecting flush explant of height 15-40 cm without wound and cracking; sterilizing the explant through first washing with washing powder and then sterilization with mercuric chloride in 0.1 % concentration for 1-2 min and thrice rinsing with bacteria-free water; soaking the explant with solution of cysteine or ascorbic acid to prevent browning; bushy bud introduction and secondary proliferation; and rooting culture with preferably seedling of 2 cm height. The said process with flush explant can propagate excellent seedling of clonic line and the present invention has wide application foreground.

The invention relates to a method for preparing coniferous polypentenol by adopting the solvent extraction method and the molecular distillation method; fresh pine branch tender leaves are dried and smashed into pine needle powder with 16 to 30 meshes as a raw material, petroleum ether is used for extraction, then the freezing dewaxing is carried out at the temperature of 0 to 25 DEG C, and a polyprenol crude product is prepared by concentration, saponification and extraction; the polyprenol crude product is separated and purified through a first-stage molecular distillation device and a second-stage molecular distillation device, thereby preparing a high-purity polyprenol finished product. The raw material is sufficient, the solvent extraction method and the molecular distillation method are projects with high efficiency, energy conservation and environmental protection; the prepared product has no solvent residue, and the purity thereof is high, thereby being in line with the modern requirements of green and environmental protection; and the method can not only improve the purity and the yield of the polyprenol, but also can be in line with the industrial production requirements, improve the traditional process and realize the clean production. The polyprenol product produced by the technology has higher purity and can be widely used in health care foods, nutrition supplements and bio-pharmaceutical industry.

The invention discloses a preparation method of fragrant preserved ducks in rice wine sauce and relates to the field of a food processing technology. The preparation method comprises the following steps: selection of raw materials, smearing of seasoning, bagging and preserving, hanging and air drying, binding with straw, smoking, cleaning, packaging and the like. According to the invention, the problem that shelf life of fragrant preserved ducks is short in the prior art can be solved.

The present invention relates to a seedling method for cyclocarya paliurus seeds. The method is characterized in comprising the following steps: a. seed selecting; b. seed treating; c. seed germination accelerating; and d. seed seedling. According to the method provided by the present invention, the seedlings emerge in 30-60 days after sowing of the cyclocarya paliurus seeds, the emergence rate is over 90%, and the seedling survival rate is over 95%.

The invention belongs to the field of biotechnology. The invention discloses a method for identifying soybean resources resistant to race 4 of soybean cyst nematode and a special SSR primer. The SSR marker for identifying resistance to race 4 of soybean cyst nematode provided by the invention is composed of Satt533 and Satt504. The method for identifying soybean resistance and susceptibility includes such step as carrying out experiments on soybean resistance and susceptibility resources from home and abroad, carrying out identification on that soybean resistance and susceptibility resources,carrying out identification on the soybean resistance and susceptibility resources, carrying out identification on the soybean resistance and susceptibility resources, and carrying out identificationon the soybean resistance and susceptibility resources. Compared with the identification method of diseased soil, the method has the advantages of saving time and labor, not limited by seasons, and has a better application prospect. It is better to serve the identification and evaluation of soybean cyst nematode race 4 in production practice. As such, that SSR mark combination and the method for identifying soybean cyst nematode resistance race 4 have wide application prospect in soybean cyst nematode resistance race 4 resource screening, variety breeding, and the like.

The invention discloses a special primer combination for quickly detecting polyploid of a willow tree and a detection method for the polyploidy. A primer provided by the invention is used for performing PCR amplification on a DNA template of the to-be-detected willow tree; by means of electrophoresis detection of an amplification product, if the number of alleles detected on amplification sites of any pair of primers of the detected sample is greater than 2, the sample is the polyploid. Compared with the existing willow tree polyploid detection method, the special primer combination which is provided by the invention and is used for willow tree polyploid screening has the advantages of simple and quick operation method and reliable result, can detect a large quantity of samples within short time, and supplies efficient and reliable technical measures to willow tree polyploid screening. An important technical support is supplied to willow tree polyploid cultivation; the special primer combination has extremely high actual application values, can achieve relatively high economic and social benefits, and has wide application prospects.

The invention relates to a rhizome-based Polygonatum cyrtonema Hua seedling culture method, and relates to a traditional Chinese medicinal material artificial reproduction technology. The method is characterized in that a TDZ solution or a 6KT solution is adopted to carry out soaking treatment on rhizome culture seedlings or new rhizome seedlings, the emergence rate of the obtained treated seedlings after transplanting reaches up to 58%, and the method is suitable for Polygonatum cyrtonema Hua industrialized seedling culture, the seedling emergence time is brought forward from February-March of the next year to November of the same year or even earlier, and therefore the seedling growth time is prolonged.

The invention discloses blood tofu, belongs to the technical field of food processing used for preparation of blood tofu and aims to solve the problem that preparation of existing blood tofu requiressmoking but the smoking is harmful to human health. The blood tofu of the invention is prepared from, by weight, 60-80 parts of bean curd, 8-12 parts of minced pork, 4-6 parts of fresh pig blood, 3-5parts of edible salt, 0.8-1.2 parts of Chinese prickly ash powder, 2-6 parts of peanut powder, 1-3 parts of sesame, 4-6 parts of bamboo shoot pulp, 0.4-0.6 part of dried tangerine or orange peel, 0.4-0.6 part of dried ginger, 1-3 parts of white granulated sugar, 1-6 parts of honey, and 2-8 parts of cooking wine. By quickly dehydrating blood tofu at high temperature firstly and then baking at low-temperature, the cooking wine can react with the white granulated sugar and the honey to generate aromatic ester substances, so that the blood tofu has fragrance; in addition, the white sugar and the honey can be colored in the baking process to endow the blood tofu with good sense; and smoking is not needed in the whole preparation process, so that the preparation method is environmentally friendly, and the problem that a large number of harmful ingredients are generated in the traditional smoking process is completely eradicated.

The invention discloses a method for cultivating vallisneria natans seedlings. The method includes steps of digging a seedling bed with the depth ranging from 8 centimeters to 10 centimeters, smashing soil blocks, adding from 0.3 to 0.5 kilogram of humus soil to each square meter of seeding bed, uniformly stirring the humus soil in the seedling bed, adding water into a seedling culture field and adding bleaching powder into the water for disinfection; selecting vallisneria natans with plant heights ranging from 15 centimeters to 20 centimeters as annual seedlings, and placing and soaking the annual seedlings in a disinfection tank for 6 to 10 minutes; transplanting the annual seedlings on the seedling bed, dividing each three to five annual seedlings as a stump, spraying combination hormone liquid on the seedling bed and covering a thin film on the annular seedlings; controlling the temperature of the water of the seedling culture field to range from 15 DEG C to 30 DEG C; and removing the thin film covering the vallisneria natans seedlings, filling water into the seedling bed, keeping the seedlings in the seedling bed for 9 to 11 days and shifting the seedlings out of the seedling culture field to obtain the vallisneria natans seedlings. After the annual seedlings are transplanted in the seedling culture field for 48 to 52 days, plant heights of new vallisneria natans seedlings grown on underground stems can reach 10 centimeters. The method has the advantages that seasonal limitation on plantation of the vallisneria natans can be broken through, and shortcomings of high cost, long period, high operational requirements and the like of a tissue culture technology for the vallisneria natans can be overcome.