Indoor auxiliary sweet potato drought tolerance identification method

Abstract

The invention relates to a sweet potato drought tolerance identification method, and in particular relates to an indoor auxiliary sweet potato drought tolerance identification method, belonging to the technical field of sweet potato drought tolerance identification. The method comprises the following steps: by selecting a test tube seedling stem section which is subjected to subculture for more than or just 50 days as an explant, cutting a single section, inoculating the explant into a high-permeation identification culture medium, performing inoculation treatment for 30 days, subsequently surveying the relative root growth number coefficients of different varieties, and judging the drought tolerance grade of the varieties according to the relative root growth number coefficients of different varieties, wherein the drought tolerance of the variety of which the relative root growth number coefficient is greater than or equal to 1.0 is high, the drought tolerance of the variety of which the relative root growth number coefficient is greater than 0.5 and less than 1.0 is medium, and the drought tolerance of the variety of which the relative root growth number coefficient is smaller than or equal to 0.5 is relatively poor. The method is simple to operate, is flexible and convenient and applicable to screening on a great number of materials, can relieve the workload of field drought tolerance identification and can improve the working efficiency.

Description

technical field [0001] The invention relates to a method for identifying drought tolerance of sweet potatoes, in particular to a method for indoor auxiliary identification of drought tolerance of sweet potatoes, which belongs to the technical field of drought tolerance identification of sweet potatoes. Background technique [0002] The purpose of the identification of drought tolerance of sweet potato is mainly to screen the varieties suitable for cultivation in arid areas from the bred sweet potato varieties and to promote the breeding of new varieties with drought tolerance, high quality and high yield. Sweet potato is a diversified crop integrating new energy raw materials and processing materials. In view of the current shortage of cultivated land, in order to "not compete with others for food and land", and sweet potato is a kind of crop with strong adaptability and stress resistance. crops, inland salinity, drought and other marginal land and hills and mountains are th...

AI Technical Summary

Problems solved by technology

In order to promote the screening and identification of sweet potato drought-tolerant resources and other materials, predecessors have used shade to simulate drought treatment to identify sweet potato drought tolerance, but this requires a lot of manpower and material resources, and the workload is very large. Difficult to meet the initial screening and identification of a large number of materials

However, by studying the relationship between the changes in antioxidant physiological indicators and the drought tolerance of sweet potato under stress and control conditions, it is difficult to directly determine the drought tolerance of sweet potato with a few physiological indicators, and the detection of physiological indicators requires a relatively large workload. Various factors such as climate may affect the accuracy and repeatability of its detection, thus affecting the judgment of drought tolerance results

In conclusion, the low efficiency of drought-tolerant identification has greatly affected the screening of drought-tolerant sweetpotato materials and the process of breeding new drought-tolerant varieties.

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