A method for identifying soybean resources resistant to race 4 of soybean cyst nematode and special SSR prim

A technique for soybean cyst nematodes and cyst nematodes, which is applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve problems such as high variability, and achieve stable and reliable results without seasonal restrictions , the effect of simple operation

Active Publication Date: 2019-01-18
山西省农业科学院农作物品种资源研究所
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Problems solved by technology

The number of repeat units in microsatellites is highly variable, resulting in polymorphism at multiple loci

Method used

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  • A method for identifying soybean resources resistant to race 4 of soybean cyst nematode and special SSR prim
  • A method for identifying soybean resources resistant to race 4 of soybean cyst nematode and special SSR prim
  • A method for identifying soybean resources resistant to race 4 of soybean cyst nematode and special SSR prim

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Experimental program
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Effect test

Embodiment 1

[0044] Embodiment 1: Anti-soybean cyst nematode No. 4 race SSR marker screening

[0045] 1. Screening of a single marker

[0046] First, 16 resources (8 high-resistance resources and 8 high-sensitivity resources) were used for SSR primer screening ( figure 1 ). From 1000 pairs of SSR primers, 13 pairs of polymorphic primers with obvious differences between resistant and sensitive resources were screened out. These 13 pairs of primers were used as candidate primers.

[0047] The 13 pairs of primers were further screened by PCR amplification with 75 resources (10 resistant resources and 66 susceptible resources). By analyzing the electrophoresis bands, it was found that there were 2 markers that could effectively distinguish the resistant and sensitive resources. Each marker has the same banding pattern among the resistant resources, but a few of the susceptible resources have the same banding pattern as the resistant resources. R is recorded as the resistant band type, an...

Embodiment 2

[0066] Example 2: Identification of Soybean Cyst Nematode-resistant Race No. 4 by Marker Combination: In this example, 106 resources were used, which were provided by Shanxi Genebank. Combination of two markers: Satt533, Satt504.

[0067] METHODS: PCR amplification and detection of amplified products were performed using the resource genome DNA as a template. According to the individual plant judgment standard of the combination of Satt533 and Satt504 markers, it was confirmed whether the resource was a disease-resistant or a disease-susceptible resource.

[0068] Judgment criteria were as follows: the amplified product had both R1 and R2 characteristic bands but no S1 and S2 bands, and it was judged as a disease-resistant resource; if it had S1 or S2 or both S1 and S2, it was judged as a susceptible resource.

[0069] Result: detection spectrum such as Figure 4-Figure 5 shown. The marker combination identification results and artificial inoculation identification results ...

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Abstract

The invention belongs to the field of biotechnology. The invention discloses a method for identifying soybean resources resistant to race 4 of soybean cyst nematode and a special SSR primer. The SSR marker for identifying resistance to race 4 of soybean cyst nematode provided by the invention is composed of Satt533 and Satt504. The method for identifying soybean resistance and susceptibility includes such step as carrying out experiments on soybean resistance and susceptibility resources from home and abroad, carrying out identification on that soybean resistance and susceptibility resources,carrying out identification on the soybean resistance and susceptibility resources, carrying out identification on the soybean resistance and susceptibility resources, and carrying out identificationon the soybean resistance and susceptibility resources. Compared with the identification method of diseased soil, the method has the advantages of saving time and labor, not limited by seasons, and has a better application prospect. It is better to serve the identification and evaluation of soybean cyst nematode race 4 in production practice. As such, that SSR mark combination and the method for identifying soybean cyst nematode resistance race 4 have wide application prospect in soybean cyst nematode resistance race 4 resource screening, variety breeding, and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for identifying soybean cyst nematode-resistant No. 4 race soybean resources and special SSR primers. Background technique [0002] Soybean Cyst Nematode (SCN) is one of the devastating diseases in soybean production, the pathogen is soybean cyst nematode ( Heterodera glycines Ichinohe). There are 8 physiological races of soybean cyst nematode in my country, including No. 1, 2, 3, 4, 5, 6, 7, and 14, among which No. 4 race is the most pathogenic and mainly distributed in the Huanghuai soybean production area. The disease generally causes a yield loss of 5-10%, and in serious cases, the yield reduction of the plot can reach more than 30%, or even the grains are not harvested, and the economic loss is serious. It is estimated that the annual economic loss caused by soybean cyst nematode in the world is more than 1.62 billion US dollars. At present, the affected...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张海平陈妍
Owner 山西省农业科学院农作物品种资源研究所
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