Method for synthesizing arbutin by utilizing hairy roots of scutellaria baicalensis to convert hydroquinone
A hydroquinone and hairy root technology, applied in the field of biotransformation, can solve the problems of many by-products, high synthesis cost, and large pollution, and achieve the effects of less by-products, simple production process, and low pollution
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Embodiment 1
[0024] 1) Inoculate the hairy roots of Scutellaria baicalensis in the seed medium of Scutellaria baicalensis hairy roots at 4g / L, and culture them in the dark at 28°C at 120r / min for 18 days; Modified MS medium with sucrose;
[0025] 2) Inoculate the hairy roots of Scutellaria baicalensis cultivated in step 1) for 18 days into the culture medium at 5 g / L, at 28° C., 150 r / min, and culture in the dark for 28 days; the culture medium is added with 50 g / L sucrose modified MS medium.
[0026] 3) dissolving hydroquinone in the aqueous ethanol solution with a volume fraction of 70%, the dissolving ratio is 20 mg per 1 mL of aqueous ethanol solution, and adding it under aseptic conditions into the Scutellaria baicalensis hairy root culture solution after step 2) cultivation, The hairy root of Scutellaria baicalensis was used for transformation for 40 hours, the temperature during transformation was 25°C, and the transformation was carried out under dark conditions; the amount of hyd...
Embodiment 2
[0035] 1) Inoculate the hairy roots of Scutellaria baicalensis in the seed medium of Scutellaria baicalensis hairy roots at 5g / L, and culture them in the dark at 28°C at 100r / min for 20 days; Modified MS medium with sucrose;
[0036] 2) The fresh and tender Scutellaria baicalensis hairy root seeds that have been cultivated for 20 days in step 1) are inoculated into the culture medium at 5 g / L, 28 ° C, 100 r / min, and cultured in the dark for 20 days; the culture medium is added with 50 g / L sucrose and the improved MS medium of 10g / L glucose and 0.4mmol / L phenylalanine; The improved MS medium adopts the formula as in Example 1;
[0037] 3) dissolving hydroquinone in the aqueous ethanol solution with a volume fraction of 70%, the dissolving ratio is to dissolve 20 mg per 2 mL of aqueous ethanol solution, and add it under aseptic conditions into the Scutellaria baicalensis hairy root culture solution after step 2) cultivation, The hairy root of Scutellaria baicalensis was used fo...
Embodiment 3
[0040]Cultivate the hairy roots of Scutellaria baicalensis, and use the cultivated Scutellaria baicalensis hairy roots to transform hydroquinone into arbutin. The time for the transformation is 30 hours, and the temperature during the transformation is 20°C. The transformation is carried out under dark conditions. Hydroquinone is dissolved with ethanol aqueous solution with a volume fraction of 70% before adding, and the dissolving ratio is that every 1mL ethanol aqueous solution dissolves 20mg hydroquinone, and the addition amount of hydroquinone is every 100mL in the culture solution of Scutellaria baicalensis hairy root Add 15 mg.
[0041] The method for described cultivating Scutellaria baicalensis hairy root is:
[0042] 1) The hairy root of Scutellaria baicalensis was inoculated in the medium of hairy root of Scutellaria baicalensis at 5 g / L, and cultured for 19 days in the dark and shaking at 150 r / min to obtain the hairy root of Scutellaria baicalensis seeds. The medi...
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