Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

A method for identifying soybean cyst nematode-resistant race 4 soybean resources and special ssr primers

A soybean cyst nematode and cyst nematode technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as high variability, and achieve stable and reliable results without seasonal restrictions , the effect of simple operation

Active Publication Date: 2021-06-11
山西省农业科学院农作物品种资源研究所
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The number of repeat units in microsatellites is highly variable, resulting in polymorphism at multiple loci

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for identifying soybean cyst nematode-resistant race 4 soybean resources and special ssr primers
  • A method for identifying soybean cyst nematode-resistant race 4 soybean resources and special ssr primers
  • A method for identifying soybean cyst nematode-resistant race 4 soybean resources and special ssr primers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: Anti-soybean cyst nematode No. 4 race SSR marker screening

[0045] 1. Screening of a single marker

[0046] First, 16 resources (8 high-resistance resources and 8 high-sensitivity resources) were used for SSR primer screening ( figure 1 ). From 1000 pairs of SSR primers, 13 pairs of polymorphic primers with obvious differences between resistant and sensitive resources were screened out. These 13 pairs of primers were used as candidate primers.

[0047] The 13 pairs of primers were further screened by PCR amplification with 75 resources (10 resistant resources and 66 susceptible resources). By analyzing the electrophoresis bands, it was found that there were 2 markers that could effectively distinguish the resistant and sensitive resources. Each marker has the same banding pattern among the resistant resources, but a few of the susceptible resources have the same banding pattern as the resistant resources. R is recorded as the resistant band type, an...

Embodiment 2

[0066] Example 2: Identification of Soybean Cyst Nematode-resistant Race No. 4 by Marker Combination: In this example, 106 resources were used, which were provided by Shanxi Genebank. Combination of two markers: Satt533, Satt504.

[0067] METHODS: PCR amplification and detection of amplified products were performed using the resource genome DNA as a template. According to the individual plant judgment standard of the combination of Satt533 and Satt504 markers, it was confirmed whether the resource was a disease-resistant or a disease-susceptible resource.

[0068] Judgment criteria were as follows: the amplified product had both R1 and R2 characteristic bands but no S1 and S2 bands, and it was judged as a disease-resistant resource; if it had S1 or S2 or both S1 and S2, it was judged as a susceptible resource.

[0069] Result: detection spectrum such as Figure 4-Figure 5 shown. The marker combination identification results and artificial inoculation identification results ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
volumeaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the field of biotechnology and discloses a method for identifying soybean cyst nematode-resistant No. 4 race soybean resources and special SSR primers. The SSR markers identified against race 4 of soybean cyst nematode provided by the present invention consist of Satt533 and Satt504. The invention is verified through experiments on resistant and susceptible soybean resources from home and abroad, and the identification method of the invention is simple to operate, economical and efficient, and the result is stable and reliable. Compared with the diseased soil identification method, it has the advantages of saving time and effort, and is not limited by seasons. It has a better application prospect and better serves the identification and evaluation of soybean cyst nematode resistant race 4 in production practice. Therefore, the combination of SSR markers and the identification method for soybean cyst nematode-resistant race 4 of the present invention will have broad application prospects in the screening of soybean cyst nematode-resistant race 4 resources and variety breeding.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for identifying soybean cyst nematode-resistant No. 4 race soybean resources and special SSR primers. Background technique [0002] Soybean Cyst Nematode (SCN) is one of the devastating diseases in soybean production, the pathogen is soybean cyst nematode ( Heterodera glycines Ichinohe). There are 8 physiological races of soybean cyst nematode in my country, including No. 1, 2, 3, 4, 5, 6, 7, and 14, among which No. 4 race is the most pathogenic and mainly distributed in the Huanghuai soybean production area. The disease generally causes a yield loss of 5-10%, and in serious cases, the yield reduction of the plot can reach more than 30%, or even the grains are not harvested, and the economic loss is serious. It is estimated that the annual economic loss caused by soybean cyst nematode in the world is more than 1.62 billion US dollars. At present, the affected...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张海平陈妍
Owner 山西省农业科学院农作物品种资源研究所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com