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Rapid propagation method for dendrobium officinale stem tissue culture

A Dendrobium officinale, tissue culture and rapid propagation technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of difficulty in obtaining explant materials, poor plant growth, large amount of materials, etc. Restricting and reducing tissue culture production costs and the effect of a large amount of materials

Active Publication Date: 2014-06-25
黄怀毅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although seeds used as explants have the advantages of simple operation, high survival rate, and rapid number expansion, the difficulty of obtaining explant materials has become a great limiting factor, and the offspring propagated by seeds may mutate; Using young stem tips, axillary buds or young stem segments as materials will have extremely adverse effects on plant growth, and is also limited by seasons
The present invention uses the adult stem section of Dendrobium candidum in the growth period of more than one year as the explant material for tissue culture and rapid propagation, and simultaneously overcomes the defects of the above two explant materials, not only the amount of material is large, but also It can be collected in any season, and there is no relevant report so far

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Dendrobium officinale stem section tissue culture rapid propagation method comprises the following steps:

[0025] 1. Preparation of stem section explants: take the stem section of Dendrobium officinale more than three nodes away from the root in the 1-year growth period, remove the leaves, and rinse with running water;

[0026] 2. Disinfection of stem explants: Take out the cleaned stem explants, put them in a beaker, soak them in 0.1% mercuric chloride solution for 5 minutes, rinse them with sterile water for 5 times, put them on an ultra-clean workbench, and cut them into lengths 1-2cm small stem segments with 2-3 nodes, then soaked in 0.1% mercuric chloride solution for 3 minutes, washed 5 times with sterile water, and set aside;

[0027] 3. Induction culture: inoculate the sterilized small stem segments into sterile induction medium, the medium formula is: MS+6-BA0.8mg / L+NAA0.8mg / L, culture conditions: temperature 24-26 ℃, fluorescent lamp irradiation 24 hours / day...

Embodiment 2

[0035] Dendrobium officinale stem section tissue culture rapid propagation method comprises the following steps:

[0036] 1. Preparation of stem section explants: take the stem section of Dendrobium officinale that is more than three nodes away from the root in the 2-year growth period, remove the leaves, and rinse with running water;

[0037] 2. Disinfection of stem explants: Take out the cleaned stem explants, put them in a beaker, soak them in 0.1% mercuric chloride solution for 5 minutes, rinse them with sterile water 4 times, put them on a clean bench, and cut them into lengths 1-2cm small stem segments with 2-3 nodes, then soaked in 0.1% mercuric chloride solution for 3 minutes, washed 4 times with sterile water, and set aside;

[0038] 3. Induction culture: Inoculate the sterilized small stem segments into sterile induction medium, the medium formula is: MS+6-BA1.0mg / L+NAA1.0mg / L, culture conditions: temperature 24-26 ℃, fluorescent lamp irradiation 24 hours / day, light...

Embodiment 3

[0046] Dendrobium officinale stem section tissue culture rapid propagation method comprises the following steps:

[0047] 1. Preparation of stem section explants: take the stem section of Dendrobium officinale that is more than three nodes away from the root in the 3-year growth period, remove the leaves, and rinse with running water;

[0048] 2. Disinfection of stem explants: Take out the cleaned stem explants, put them in a beaker, soak them in 0.1% mercuric chloride solution for 5 minutes, rinse them with sterile water 4 times, put them on a clean bench, and cut them into lengths 1-2cm small stem segments with 2-3 nodes, then soaked in 0.1% mercuric chloride solution for 3 minutes, washed 5 times with sterile water, and set aside;

[0049] 3. Induction culture: Inoculate the sterilized small stem segments into a sterile induction medium, the medium formula is: MS+6-BA1.2mg / L+NAA1.2mg / L, culture conditions: temperature 24-26 ℃, fluorescent lamp irradiation 24 hours / day, lig...

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Abstract

The invention discloses a rapid propagation method for dendrobium officinale stem tissue culture, and belongs to the technical field of biology. According to the rapid propagation method, stem sections (above the third section from the root) of dendrobium officinale with the growth period more than one year are used for carrying out rapid propagation for the tissue culture. The rapid propagation method comprises the following steps of: preparation of implants except the stems, sterilization, induction culture, subculture, strong seedling culture, rooting culture and light culture hardening-seedling. The rapid propagation method for the dendrobium officinale stem tissue culture, provided by the invention, has the advantages of easiness of material obtaining, large material amount and no limitation on the seasons; the maternal biological property can be kept well; a culture technology is simple and easy to operate and according to the method, the low pollution rate and the rapid propagation speed can be realized; and the rapid propagation method for the dendrobium officinale stem tissue culture has the important meanings on resource regeneration and continuous use of scarce medicinal plants in imminent danger.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a plant cultivation method, in particular to a method for tissue culture and rapid propagation of dendrobium officinale stem segments. Background technique [0002] Dendrobium officinale Kimura et Migo belongs to the epiphytic orchids of Orchidaceae. , The effect of moisturizing the throat and improving eyesight. Modern medical research shows that the polysaccharides, alkaloids, amino acids and other components of Dendrobium officinale can improve immune function, inhibit thrombosis, inhibit tumors and delay aging. Due to the small size of Dendrobium candidum seeds, there is post-ripening phenomenon in the seed embryos. When the fruit matures, the development of the seed embryos does not exceed the spherical stage, the seeds lack endosperm, and the existence of symbiotic bacteria is required. Therefore, the reproductive rate is extremely low under natural conditions. Years of unrestr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 黄威祥黄怀毅农定霖
Owner 黄怀毅
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