A culture medium composition adaptable test-tube seedling rooting of carnation and application thereof

A medium and a technology of carnation, applied in the field of plant tissue culture, can solve problems such as unfavorable large-scale industrial production, high concentration of medium components, expensive cultivation cost, etc., and achieves reduction of oxidative pollution of the environment and water body, simple formula, and reduced Effects of Environmental Hormone Pollution

Inactive Publication Date: 2007-04-25
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved by the present invention is to provide a medium composition suitable for carnation test-tube seedling rooting and its application, to overcome the need for more than one hormone in the existing carnation rooting medium and the high concentration of medium components and the cost of cultivation. Defects that are expensive, easy to cause pollution, and unfavorable for large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] In this example, test-tube plantlets of carnation were inoculated on rooting medium supplemented with different types and concentrations of auxins, the rooting rate and the quality of regenerated roots were compared, and the best rooting medium was selected.

[0037] 1. Test material: The carnation variety used for the test is: "0420" Mary, provided by Shanghai Flower Breed Farm.

[0038] 2. Medium and culture conditions

[0039] The rooting medium is: 1 / 2MS medium is the basal medium plus 10 kinds of hormones shown in Table 2. The sucrose concentration of the medium is 3.0%, the agar concentration is 0.7%, the pH value is 5.8, the culture temperature is 25±2°C, the light intensity is 1500-2000lx, and the light time is 16h / d.

[0040]Hormone components and culture results in the rooting medium of table 2

[0041] hormone

Concentration (mg / L)

Rooting rate (%)

Average number of roots (bars)

root length

Amount of lateral root

0

...

Embodiment 2

[0054] In this embodiment, the test-tube seedlings of three other carnation varieties other than "0420" Mary are inoculated on the rooting medium with different types and concentrations of auxin as shown in Table 2, and the rooting rate and the quality of the regenerated root are compared, and the test is carried out. Suitability of rooting medium for different cultivars.

[0055] 1. Test materials: The three carnation varieties tested are: "0409" Cherry Blossom, "0413" Magnolia and "0418" Poppy, provided by Shanghai Flower Breeding Farm.

[0056] 2. Culture medium, culture condition and test method are the same as embodiment 1.

[0057] 3. Observe the test phenomenon and analyze the results:

[0058] The medium suitable for the rooting of the test-tube seedlings of the above three Carnation varieties is IAA0.1, 0.5 and 1.0mg / L and IBA0.1, 0.5 and 1.0mg / L among the above 10 rooting mediums. It is 1 / 2MS+IAA0.1mg / L.

[0059] The test-tube seedlings of three carnation varietie...

Embodiment 3

[0061] In this example, carnation test-tube seedlings were inoculated on 1 / 2 concentration of LS medium and White medium, and different types and concentrations of auxin were added to compare the rooting rate and the quality of regenerated roots, and test the applicability of different basic medium .

[0062] 1. Test material: The carnation variety used for the test is: "0420" Mary, provided by Shanghai Flower Breed Farm.

[0063] 2. Medium:

[0064] The rooting medium is based on 1 / 2LS medium or White as the base medium plus 10 kinds of hormones and 20 kinds of medium shown in Table 2. The concentration of sucrose in the medium is 3.0%, the concentration of agar is 0.7%, and the pH value is 5.8.

[0065] 3. Culture condition and test method are the same as embodiment 1.

[0066] 4. Observe the test phenomenon and analyze the results:

[0067] The culture medium of the above-mentioned 20 kinds of carnation test-tube seedlings are all suitable for rooting.

[0068] Carnati...

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PUM

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Abstract

The invention relates to cultivate medium composite, used for induce germ at bamboo. Wherein, it adds IAA 0.1-1.0mg/L or IBA 1.0mg/L into semi-density MS cultivate medium. The invention points out that growth adjuster IAA can accelerate the germ induction of bamboo. The invention also provides an application of said composite to cultivate the bamboo organism.

Description

technical field [0001] The invention belongs to the field of plant tissue culture. Background technique [0002] Carnation (Dianthus Caryophyllus) is also known as carnation and carnation. It is a perennial herbaceous plant of the family Caryophyllaceae, native to southern Europe. It has delicate stems and leaves, rich and elegant flowers, gorgeous and delicate colors, high ornamental value, and a single flower with a long flowering period. It is suitable for making bouquets and flower baskets. It is one of the four famous cut flowers in the world. [0003] Carnation is not easy to reproduce by seeds, and sexual reproduction is prone to hybridization between varieties, which cannot maintain the good quality of the parents. Cuttings are often used for reproduction in production. However, due to the low propagation coefficient and slow speed of cuttings, it is difficult to meet the needs of the market, especially for some newly bred and newly introduced varieties. Due to the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘丽娜魏秀丽逯慧李建粤
Owner SHANGHAI NORMAL UNIVERSITY
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