Culture method for inducing differentiation of umbilical cord mesenchymal stem cell into cartilage and culture medium used in method
A mesenchymal stem cell and culture medium technology, applied in the field of cell induction culture, can solve the problems of low differentiation rate, fast dedifferentiation speed, inability of mesenchymal stem cells to differentiate into chondrocytes, etc., and achieve the effect of increasing differentiation rate and induction speed
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0045] 1. Inducing components
[0046] name
components
TGF-β3
7ng / mL
0.07μmol / L
Ascorbate
40μg / mL
1×ITS
1.3%
L-proline
30μg / mL
sodium pyruvate
1.3mmol / L
[0047] 2. Preparation of induction medium
[0048] 1) The basal medium for inducing differentiation is DMEM high-glucose medium.
[0049]2) Calculate the amount of induction components TGF-β3, dexamethasone, ascorbate, 1×ITS, L-proline, and sodium pyruvate by preparing 100 mL of complete induction medium, and pack them in sterile 0.5 mL EP tubes. Store at -20°C. All components were protected from light.
[0050] 3) When preparing, take out the required volume of DMEM high-glucose medium, dexamethasone, ascorbate, 1×ITS, L-proline, sodium pyruvate, dissolve and mix to make a premix, which can be stored at 4°C 5-7d.
[0051] 4) Before use, add TGF-β3 to make a complete medium, and try to use up the complete medium within 24 ho...
Embodiment 2
[0066] 1. Inducing components
[0067] name
components
TGF-β3
13ng / mL
0.13μmol / L
Ascorbate
60μg / mL
1×ITS
0.7%
L-proline
50μg / mL
0.7mmol / L
[0068] 2. Preparation of induction medium
[0069] With embodiment 1.
[0070] 3. Induction of Differentiation Steps
[0071] With embodiment 1.
Embodiment 3
[0073] 1. Inducing components
[0074]
[0075]
[0076] 2. Preparation of induction medium
[0077] With embodiment 1.
[0078] 3. Induction of Differentiation Steps
[0079] With embodiment 1.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com