Method for inducing mononuclear cells to differentiate into dendritic cells

Abstract

The invention relates to the technical field of cell culture, in particular to a method for inducing mononuclear cells to differentiate into dendritic cells. The method comprises the following steps: adding CD14+ mononuclear cells into a complete culture medium I, conducting culturing for 4-5 days, adding the cultured system into a complete culture medium II, and conducting culturing for 1-2 days to obtain the dendritic cells. By controlling the sorting method of the CD14+ mononuclear cells, the number of the non-CD14+ mononuclear cells in the adhesion cells and the PBMC is effectively reduced, the content of the cells capable of being differentiated into the dendritic cells is increased, and meanwhile higher dendritic cells can be obtained under the conditions of short time and low cell factor content. GM-CSF or interleukin can be used as a cell factor to promote differentiation of dendritic cells, when GM-CSF or interleukin, such as interleukin-4, is purely added, the induction rate of DC cells is low, and when GM-CSF and interleukin-4 act together, the induction rate is high and the differentiation time is shortened under the condition of low final concentration of the cell factor.

Description

technical field [0001] The invention relates to the technical field of cell culture, and more specifically, the invention relates to a method for inducing monocytes to differentiate into dendritic cells. Background technique [0002] Dendritic cells (DC) are named for their stellate pleomorphism or dendritic processes on their surface. Dendritic cells (DC) are professional antigen-presenting cells with the strongest function in the body. cells, APC), which can efficiently uptake, process and present antigens, immature DC has a strong ability to migrate, mature DC can effectively activate initial T cells, and is at the center of initiating, regulating, and maintaining immune responses. [0003] DCs originate from bone marrow pluripotent hematopoietic stem cells, and there are two main ways of differentiation: ① Myeloid stem cells differentiate into DCs under the stimulation of GM-CSF, called myeloid dendritic cells (MDCs), also known as DC1, and single Nuclear cells and gran...

AI Technical Summary

Problems solved by technology

[0004] At present, DC cells are mainly obtained by differentiation of monocytes extracted from peripheral blood and umbilical cord blood by adding cytokines, and the induction rate of DCs obtained after differentiation is generally low, and more cytokines need to be added, the operation is cumbersome, and it is used for differentiation. Monocytes often contain components that cannot differentiate or differentiate into other cells, and can also affect the differentiation of dendritic cells

Images