System for inducing nerve stem cell differentiation and inducing method thereof

Abstract

The invention discloses a method for preparing neural stem cells, which comprises a step of inductively culturing P19 cells in N2B27 culture medium to obtain a cell group containing neural stem cells above 94%. The method can culture the P19 cells in an environment without blood serum and retinoic acid, thus suppressing the interference of the blood serum with complex components and preventing the obtained neural stem cells from being transformed posteriorly by retinoic acid. Moreover, the method can directly transform pluripotent stem cells to neutral stem cells without resulting in selective cell apoptosis. The neutral stem cells obtained by the invention have anterior neutral plate characteristics and totipotency, and can well simulate the neurogenesis process in body. Accordingly, the neural stem cells can be used as the research model for analyzing neural induction and neural differentiation process from epiblast to neuroderm, thus providing an ideal path for researching development of embryo after nidation.

Description

System and induction method for inducing neural stem cell differentiation technical field The present invention belongs to the fields of biotechnology and cell biology, and more particularly, the present invention relates to a method for inducing differentiation of neural stem cells and neural stem cells obtained by the method. Background technique Early embryonic development in mice is a complex process. In early pregnancy (E2.5-E4.5), mouse embryos consist of trophectoderm (Trophectoderm) and inner cell mass (ICM). As development progresses, ICM cells undergo reorganization to form another population of pluripotent epithelioid cells, the epiblast (E4.5-E6.5). The cells of epiblast go through gastrulation to form three germ layers (E6.5-E7.5) of mouse embryo, namely ectoderm (Ectoderm), mesoderm (Mesoderm) and endoderm (Endoderm). Embryos subsequently develop into the organogenesis stage (E7.5-E12.5). During this period, the development of the nervous system was the ea...

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Problems solved by technology

And the neural stem cells induced by RA have limited developmental potential and can only form limited neural cell types

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