7 results about "Neuroectoderm" patented technology

Methods for differentiating pluripotent stem cells to neuroectoderm in dynamic suspension culture using small molecule or protein inhibitors of TGFβ / Activin / Nodal signaling and BMP signaling are provided. Also provided are methoc and protocols for differentiating pluripotent stem cells such as human embryonic stem cells first to neuroectoderm, then further to glial progenitor cells, and further to oligodendrocyte progenitor cells (OPCs), and compositions obtained thereby. The methods of the present disclosure reproducibly produce neuroectoderm progenitor cells by day 7 of the differentiation process, glial progenitor cells by day 21 of the differentiation process and OPCs by day 42 of the differentiation process.

The invention relates to the technical field of cell biology, in particular to a culture medium for inducing neural stem cells and application thereof.The culture medium for inducing the neural stem cells comprises a neural ectoderm induction culture medium and a neural stem cell induction culture medium; the neural ectoderm induction culture medium is prepared from a basic culture medium, an N-2 additive, a B-27 additive, an inhibitor SB431542 and an inhibitor Dorsomorphin, the neural stem cell induction culture medium is prepared from a basic culture medium, an N-2 additive and a B-27 additive, the basic culture medium is composed of a DMEM / F12 culture medium, a Neurobase culture medium, GlutaMax and non-essential amino acid NEAA. The problems that neural stem cells obtained through an existing single-layer adherent induction method based on small molecules or growth factors are uneven in differentiation and low in purity are solved.

The application discloses a culture solution for indirectly inducing human induced pluripotent stem cells into mesenchymal stem cells, which is composed of a neuroectoderm induction culture solution, a mesenchymal stem cell differentiation culture solution and a mesenchymal stem cell maintenance culture solution. The neuroectoderm induction culture solution is composed of a Neurobasal culture medium, a DMEM / F12 culture medium, an N2 additive, a B27 additive, a GSK3 inhibitor and a TGF-beta inhibitor. The application further discloses a method for indirectly inducing human induced pluripotent stem cells into mesenchymal stem cells, which comprises the following steps: taking the induced pluripotent stem cells, culturing the induced pluripotent stem cells in the neuroectoderm induction culture solution for 6 days, then culturing the induced pluripotent stem cells in the mesenchymal stem cell differentiation culture solution for 7 days, and then culturing the induced pluripotent stem cells in the mesenchymal stem cell maintenance culture solution for 3-5 generations. The method can direct the induced pluripotent stem cells to differentiate into mesenchymal stem cells in a short time, and greatly shortens the culture time.

This invention belongs to the field of regenerative medicine and relates to cell therapy, specifically providing pluripotent stem cell-derived RPE cells, formulations, pharmaceutical compositions, reagents, methods, and kits. Obtaining RPE cells by differentiation of iPSCs is achieved by adding small chemical molecules as inhibitors or activators to E6 medium and includes a neuroectoderm cell induction phase, an RPE progenitor cell induction phase, an RPE cell induction phase, and an RPE cell maturation phase. In this differentiation process, there is no need to manually remove heterogeneous cells with non-RPE-like morphology, and pure RPE cells can be obtained by subculturing.

The invention provides an in-vitro cerebral cortex intermediate neuron precursor cell population as well as a preparation method and application of the in-vitro cerebral cortex intermediate neuron precursor cell population. According to the cell population, the iNSC is taken as an initial cell to directionally differentiate to the neuroectoderm cell, so that neuroendoderm and neuromesoderm impurity cells generated in the differentiation process can be reduced, the cell differentiation direction is more accurate, and the differentiated cell purity is higher. The cell population can be used for the prevention, diagnosis and treatment of nervous system diseases.

To provide induction of neural progenitor cells, oligodendrocyte progenitor cells, and oligodendrocytes by stem cell differentiation using landmark transcription factors.SOLUTION: Novel methods of inducing human iPSC-derived neural progenitors, oligodendrocyte progenitors, oligodendrocytes with unprecedented efficiency and functionality. The core of the present invention is the use of experimentally discovered transcription factors in a previously unknown manner at key differentiation decision points along the pathway from pluripotency to ectoderm to neuroectoderm to NPCs to OPCs to oligodendrocytes.SELECTED DRAWING: None

The invention provides a craniofacial skeleton organoid controlled by sensory organs and sensory nerves and a preparation method thereof, and relates to the technical field of biologication.The preparation method comprises the steps that in a three-dimensional matrix, human pluripotent stem cells are induced through morphogenetic gradient driving to form nerve ectoderm with a nerve tube head-tail axis and a nerve tube back-abdominal axis; the neural ectoderm is respectively differentiated into a neural crest cell flow carrying head-tail axis position information, a visual cranium substrate-like structure carrying head-tail axis position information, an olfactory cranium substrate-like structure carrying head-tail axis position information and a trigeminal nerve cranium substrate-like structure carrying head-tail axis position information; and a craniofacial skeleton organ is formed under mediation of nerve cadherin. The technical problem that in the prior art, a method for forming a craniofacial skeleton organ through induction cannot form a sensory organ and a sensory nerve innervation function is solved.