Methods for differentiating pluripotent stem cells in dynamic suspension culture

A technology of human pluripotent stem cells and dynamic suspension, which is applied in the field of cell biology, neuroectoderm and glial lineage cells, and can solve problems such as not easy to expand
CN113646422APending Publication Date: 2021-11-12LINEAGE CELL THERAPEUTICS INC
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
LINEAGE CELL THERAPEUTICS INC
Publication Date
2021-11-12

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Abstract

Methods for differentiating pluripotent stem cells to neuroectoderm in dynamic suspension culture using small molecule or protein inhibitors of TGF[beta] / Activin / Nodal signaling and BMP signaling are provided. Also provided are method and protocols for differentiating pluripotent stem cells such as human embryonic stem cells first to neuroectoderm, then further to glial progenitor cells, and further to oligodendrocyte progenitor cells (OPCs), and compositions obtained thereby. The methods of the present disclosure reproducibly produce neuroectoderm progenitor cells by day 7 of the differentiation process, glial progenitor cells by day 21 of the differentiation process and OPCs by day 42 of the differentiation process.
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Description

technical field

[0001] The present disclosure relates to the fields of cell biology and neuroectodermal and glial lineage cells (eg, oligodendrocyte progenitor cells). More specifically, the present disclosure relates to novel methods for differentiating pluripotent stem cells into neuroectoderm in dynamic suspension culture using small molecule or protein inhibitors of TGFβ / Activin / Nodal signaling and BMP signaling. The present disclosure further provides novel methods for differentiating pluripotent stem cells (eg, human embryonic stem cells) first into neuroectoderm, then further into glial progenitor cells, and further into oligodendrocyte progenitor cells. The present disclosure further relates to neuroectodermal cells, glial progenitor cells and oligodendrocyte progenitor cells expressing one or more markers produced by the methods according to the present invention. Background technique

[0002] Oligodendrocyte progenitor cells (OPCs) are a subset of glial cells in t...

Claims

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