Tightening and anti-wrinkle composition, face cream and preparation method of face cream

Abstract

The invention discloses a firming and anti-wrinkle composition, face cream and a preparation method of the face cream, and particularly belongs to the field of preparations for dressing and makeup. The firming and anti-wrinkle face cream comprises a firming and anti-wrinkle composition, composite polypeptide, recombinant collagen, a skin conditioner and the like, and the firming and anti-wrinkle composition comprises a leontopodium alpinum flower / leaf extract, a peach resin extract, a radix gentianae extract and a trollius chinensis bunge extract. The composite polypeptide is at least two of palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, acetyl hexapeptide-8 and palmitoyl hexapeptide-12, and the recombinant collagen is at least one of recombinant XVII type collagen, recombinant I type collagen, recombinant IV type collagen, recombinant V type collagen and recombinant VII type collagen. The firming and anti-wrinkle face cream provided by the invention can effectively improve the elasticity value of skin, can remarkably improve the firming degree of the skin, and has excellent anti-wrinkle and anti-aging effects.

Description

Technical Field

[0001] The invention belongs to the field of cosmetic preparations, and in particular relates to a firming and anti-wrinkle composition, a facial cream and a preparation method thereof. Background Art

[0002] With the increasing demand for skin health and beauty, firming and anti-wrinkle have become important research directions in the field of cosmetics and skin care products. In existing firming and anti-wrinkle products, the combined use of multiple ingredients has become a trend. These ingredients usually include plant extracts, peptides, vitamins, etc., which achieve firming and anti-wrinkle effects through different mechanisms of action, such as promoting collagen production, inhibiting free radical production, and improving skin microcirculation. However, these compositions still have many deficiencies in formula design, ingredient selection, and mechanism of action, such as unclear interactions between ingredients, poor stability, and insignificant effects.

[0003] Although existing firming and anti-wrinkle products have made certain progress, they still face the following problems and challenges: First, the interaction between ingredients is unclear. Traditional compositions often lack in-depth research on the interaction between ingredients, making it difficult to achieve the best effect; Second, poor stability. Some ingredients are prone to chemical reactions or degradation under specific conditions, resulting in poor product stability and affecting the use effect. For example, Chinese patent CN117205113A discloses a firming and anti-wrinkle composition, including components: black truffle extract, hydroxypropyl tetrahydropyrantriol, ylang-ylang flower oil-3, Australian mahogany essential oil, bay leaf oil and emulsifier. Since the composition contains essential oil components, it is easy to decompose and volatilize under specific conditions, affecting the use; Third, the effect is not significant. Due to the limitations of ingredient selection and mechanism of action, existing products often find it difficult to achieve significant firming and anti-wrinkle effects and cannot meet the needs of consumers. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention aims to provide a firming and anti-wrinkle composition containing alpine edelweiss flower / leaf extract, peach resin extract, gentian root extract, and nasturtium extract, as well as a facial cream containing the composition, a composite polypeptide, and recombinant collagen. The ingredients are scientifically proportioned to achieve a synergistic effect, thereby significantly improving the firming and anti-wrinkle effect of the overall formula.

[0005] To achieve the above object, the present invention discloses the following technical solutions: In a first aspect, the present invention provides a firming and anti-wrinkle composition, which comprises the following components in parts by mass: 2-5 parts of alpine edelweiss flower / leaf extract; 1-6 parts of peach resin extract; Gentiana root extract 0.5-0.7 parts; 1-4 parts of nasturtium flower extract.

[0006] Preferably, the preparation method of the nasturtium flower extract comprises the following steps: S1-1. Take nasturtium and crush, sieve, and obtain nasturtium powder; S1-2. Deionized water was added to the nasturtium powder, ultrasonically treated for 20-30min, and the filtrate A and the residue A were filtered to obtain a standby; S1-3. Add ≥95 v / v% ethanol aqueous solution to the residue A, reflux and extract, and filter to obtain filtrate B; S1-4. After combining filtrate A and filtrate B, the filtrate is concentrated and dried to obtain nasturtium flower extract.

[0007] Further preferably, the mesh size of the sieving in step S1-1 is 60 meshes.

[0008] Further preferably, in step S1-2, the mass ratio of nasturtium powder to deionized water is 1:10, and the frequency of ultrasound is 28 kHz.

[0009] Further preferably, in step S1-3, the amount of ethanol aqueous solution added is 5 times the mass of the filter residue, the temperature of reflux extraction is controlled at 85° C., the number of refluxes is 3 times, and each reflux is 0.7 h.

[0010] In a second aspect, the present invention provides use of the firming and anti-wrinkle composition described in the first aspect in the preparation of a toner, essence, gel, lotion and / or cream with firming and anti-wrinkle effects.

[0011] In a third aspect, the present invention provides a facial cream with firming and anti-wrinkle effects, wherein the facial cream comprises the firming and anti-wrinkle composition of the first aspect, and the amount of the firming and anti-wrinkle composition added to the facial cream is ≤3wt%.

[0012] Preferably, the facial cream further comprises a composite polypeptide, recombinant collagen, a skin conditioner, a thickener, an emulsifier, a moisturizer, a preservative, a chelating agent, a moisturizer, and a solvent.

[0013] Further preferably, the composite polypeptide is at least two of palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, acetyl hexapeptide-8, dipeptide diaminobutyryl benzylamide diacetate, carnosine, and nonapeptide-1; The recombinant collagen is at least one of recombinant type XVII collagen, recombinant type I collagen, recombinant type IV collagen, recombinant type V collagen, and recombinant type VII collagen; The skin conditioning agent is at least one of polydeoxyribonucleotide, nicotinamide, caprylhydroxamic acid, and 3-o-ethyl ascorbic acid; The thickener is at least one of sodium polyacrylate, acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, xanthan gum, carbomer, and polyacrylamide; The emulsifier is at least one of PEG-20 methyl glucose sesquistearate, glyceryl stearate, sorbitan stearate, PEG-100 stearate, and cetearyl glucoside; The emollient is at least one of dicaprylyl carbonate, cetearyl alcohol, hydrogenated polyisobutylene phenyl trimethicone, tocopheryl acetate, C13-14 isoparaffin, jojoba seed oil, squalane, caprylic / capric triglyceride, ethylhexyl palmitate, avocado butter, dimethicone, lavender oil, grapefruit peel oil, ylang-ylang flower oil, lemon peel oil, and damascena flower oil; The preservative is at least one of p-hydroxyacetophenone, methylparaben, caprylhydroxamic acid, glyceryl caprylate, 1,2-pentanediol, phenoxyethanol, ethylhexylglycerin, and caprylyl glycol; The chelating agent is at least one of disodium EDTA and tetrahydroxypropyl ethylenediamine; The moisturizing agent is at least one of β-glucan, glycerol, sodium polyglutamate, Tremella fuciformis fruiting body extract, sodium hyaluronate, allantoin, butylene glycol, propylene glycol, and 1,2-hexanediol; The solvent is deionized water.

[0014] In a fourth aspect, the present invention provides a method for preparing the facial cream with firming and anti-wrinkle effects according to the third aspect, the preparation method comprising the following steps: The firming and anti-wrinkle composition, composite polypeptide, recombinant collagen, skin conditioner, thickener, emulsifier, moisturizer, preservative, chelating agent, moisturizer and solvent are uniformly mixed to obtain the firming and anti-wrinkle facial cream.

[0015] In the present invention: Alpine edelweiss flower / leaf extract contains a variety of active ingredients, including volatile oils, flavonoids, phenylpropanoids, isobenzofuranones and terpenoids. These ingredients give it multiple functions such as antioxidant, anti-inflammatory, antibacterial and anti-inflammatory, skin barrier protection and skin brightening. It can scavenge free radicals, reduce UV damage to the skin, prevent photoaging, promote collagen production, and improve skin elasticity and firmness.

[0016] Peach resin extract is rich in polysaccharides, including arabinose, galactose, xylose, mannose, etc. In addition, peach resin extract also contains natural antioxidant active substances such as phenols and flavonoids. Peach resin extract can maintain the water balance inside and outside the skin cells, thereby achieving the effect of moisturizing; it has anti-inflammatory effects, can reduce skin inflammation and allergic reactions; it has the effect of repairing and strengthening the skin barrier, and promotes wound healing; it can promote fibroblast proliferation and type I collagen synthesis, reduce the activity of β-galactosidase, and has anti-wrinkle and skin tightening effects; the phenols and flavonoids contained have antioxidant activity, can scavenge free radicals, and reduce skin damage.

[0017] Gentiana root extract is rich in chemical components such as iridoids, triterpenes, ketones and flavonoids. It can reduce skin inflammation, resist free radical damage, inhibit microbial growth, and promote skin cell renewal and repair, thereby improving skin barrier function. In addition, Gentiana root extract can regulate sebum secretion and improve oily and rough skin conditions. It is a multi-effect skin care ingredient.

[0018] Nasturtium flower extract is rich in active ingredients such as flavonoids, alkaloids and polysaccharides, which give it a variety of skin care benefits. In cosmetics, nasturtium flower extract mainly plays an antibacterial, anti-inflammatory and antioxidant role, which can effectively inhibit bacteria on the skin surface, reduce inflammation and redness, and neutralize free radicals, protect the skin from oxidative damage, and delay skin aging.

[0019] The present invention has found that the combined use of alpine edelweiss flower / leaf extract, peach resin extract, gentian root extract and nasturtium extract can act on the skin more effectively to achieve a firming and anti-wrinkle effect, and to a certain extent slow down skin aging.

[0020] Beneficial effects of the present invention: 1. The present invention uses a compound of alpine edelweiss flower / leaf extract, peach resin extract, gentian root extract, and nasturtium extract to effectively promote collagen production, improve skin elasticity, and achieve a firming and anti-wrinkle effect on the skin; 2. The firming and anti-wrinkle cream provided by the present invention can effectively improve the elasticity value of the skin, can significantly improve the firmness of the skin, and has excellent anti-wrinkle and anti-aging effects. DETAILED DESCRIPTION

[0021] To better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. It should be understood by those skilled in the art that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0022] Unless otherwise specified, the test methods used in the embodiments and comparative examples are conventional methods; the materials, reagents, etc. used, unless otherwise specified, can be obtained from commercial channels; the percentages mentioned in the embodiments and comparative examples, unless otherwise specified, are mass percentages.

[0023] In the present invention: The alpine edelweiss flower / leaf extract is the alpine edelweiss (LEONTOPODIUM ALPINUM) flower / leaf extract, INCI name is LEONTOPODIUM ALPINUM FLOWER / LEAF EXTRACT, purchased from Shanghai Jiayu Biotechnology Co., Ltd.; Peach resin extract is peach (PRUNUS PERSICA) resin extract, INCI name is PRUNUS PERSICA (PEACH) RESIN EXTRACT, purchased from Shanghai Jiayu Biotechnology Co., Ltd.; Gentiana root extract is Gentiana scabra root extract, INCI name is GENTIANA SCABRAROOT EXTRACT, purchased from Shanghai Jiayu Biotechnology Co., Ltd.; Nasturtium is a plant of the genus Nasturtium in the family Nasturtium. Its Latin name is Tropaeolum majus L. , purchased from Guangzhou Qingping Chinese herbal medicine market, the variety has been identified; Other raw materials are commercially available.

[0024] Preparation of Nasturtium flower extract Step S1-1. Take the dried nasturtium and grind it in a high-speed grinder, pass it through a 60-mesh sieve, and obtain nasturtium powder; Step S1-2. Add 10 times the mass of deionized water to the nasturtium powder, ultrasonically treat for 20 min at 28 kHz, and filter to obtain the filtrate A and the residue A for standby; Step S1-3. Add 5 times the mass of 95v / v% ethanol aqueous solution to the residue A, and perform reflux extraction. The reflux extraction temperature is 85°C, the reflux number is 3 times, each reflux is 0.7h, and the filtrate B is obtained by filtering; Step S1-4. After combining filtrate A and filtrate B, the filtrate A and filtrate B are concentrated and vacuum dried to obtain a nasturtium flower extract.

[0025] Preparation of firming and anti-wrinkle composition The raw materials are compounded and mixed according to the formula amounts in Table 1 to obtain the firming and anti-wrinkle compositions 1-9.

[0026] Table 1 Raw materials and their mass ratios in the firming and anti-wrinkle composition Composition 1 Composition 2 Composition 3 Composition 4 Composition 5 Composition 6 Composition 7 Composition 8 Composition 9 Leontopodium Alpinum Flower / Leaf Extract 2 3 5 - 3 3 3 3 1 Peach resin extract 1 3 6 3 - 3 3 3 1 Gentiana Root Extract 0.5 0.6 0.7 0.6 0.6 - 0.6 - 1 Nasturtium flower extract 1 2.5 4 2.5 2.5 2.5 - - 1 Note: “-” in the table means no addition.

[0027] In order to evaluate the firming and anti-wrinkle performance of the firming and anti-wrinkle composition, the firming and anti-wrinkle compositions 1-9 prepared above were respectively diluted with deionized water to form test sample solutions 1-9 with a concentration of 3 wt % for performance testing.

[0028] Performance Testing Zebrafish anti-wrinkle efficacy test Method source: "Standard operating procedures for zebrafish anti-wrinkle efficacy evaluation experiment" System and sample size: Experimental system: wild-type AB strain zebrafish.

[0029] Zebrafish age: 4 days post fertilization (4 dpf).

[0030] The number of experimental samples in each group was 30 (three biological replicates, N = 3).

[0031] Sample to be tested: sample solution 1-9.

[0032] Principle and method: The growth, repair, nutrition, elasticity and tension of the skin are all related to collagen. Its loss will reduce the smoothness of the skin and produce wrinkles. In tetrapods, type I collagen is a trimer, mainly composed of two α1 chains and one α2 chain, encoded by col1a1a and col1a2 genes, respectively, and performs collagen-related biological functions in connective tissue and bone. There are three type I collagen genes in zebrafish, encoding col1a1a, col1a1b and col1a2 of α1 (I), α2 (I) and α3 (I) chains, respectively. Therefore, by detecting the relative expression of col1a1a or / and col1a1b or / and col1a2 genes, it can be indicated whether the sample to be tested has anti-wrinkle effect.

[0033] In this experiment, the anti-wrinkle effect of the composition 1-9 was detected by detecting the relative expression level of the col1a1a gene. The test results are shown in Table 2.

[0034] Zebrafish firming effect experiment Method source: "Standard operating procedures for zebrafish firming efficacy evaluation experiment" System and sample size: Experimental system: wild-type AB strain zebrafish.

[0035] Zebrafish age: 4 days post fertilization (4 dpf).

[0036] The number of experimental samples in each group was 30 (three biological replicates, N = 3).

[0037] Sample to be tested: sample solution 1-9.

[0038] Principle and method: Elastin is the main component of elastic fibers in skin tissue, which can provide structural support for the skin and protect the skin from aging and sagging. Elastin is composed of two types of short peptides arranged alternately. Eln1 and eln2 are responsible for encoding different peptides of elastin. The two genes are jointly responsible for regulating the expression level of elastin, making the skin firm and elastic. Zebrafish have elastin (eln1, eln2) genes similar to humans. Therefore, by detecting the relative expression of eln1 and / or eln2 genes, it can be shown whether the sample to be tested has a firming effect.

[0039] In this experiment, the firming effect of the compositions 1-9 was detected by detecting the relative expression level of the eln1 gene. The test results are shown in Table 2.

[0040] Table 2 Firming and anti-wrinkle test results of the composition Group Relative expression of col1a1a gene Relative expression of eln1 gene Composition 1 3.04 3.15 Composition 2 3.11 3.42 Composition 3 2.97 3.1 Composition 4 1.43 1.69 Composition 5 1.56 2.04 Composition 6 1.23 1.52 Composition 7 1.35 1.85 Composition 8 0.89 1.19 Composition 9 2.25 2.68 Result analysis: 1. The firming and anti-wrinkle composition provided by the present invention has a significant promoting effect on the expression of collagen and elastin in zebrafish; 2. From the performance test results of compositions 4-7, it can be seen that when any one of the ingredients in the firming and anti-wrinkle composition provided by the present invention is missing, the firming and anti-wrinkle performance of the composition is greatly affected, proving the key role of alpine edelweiss flower / leaf extract, peach resin extract, gentian root extract and nasturtium extract in the formula; the performance results of composition 9 show that when the ratio in the composition is not within a specific range, the performance of the composition will be reduced.

[0041] Preparation of Firming Anti-Wrinkle Cream: Weigh each raw material according to the formula in Table 3; Step S2-1, adding part of deionized water into a batching pot and heating it to 85° C., then sequentially adding sodium polyacrylate, acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, PEG-10 polydimethylsiloxane, squalane, polydimethylsiloxane, glycerin, disodium EDTA, allantoin and part of butylene glycol, homogenizing and dispersing at a speed of 1000 r / min for 30 minutes, and keeping the mixture warm for 30 minutes to obtain a mixture C; Step S2-2, heating the remaining butanediol to 60° C., adding p-hydroxyacetophenone and 1,2-hexanediol, and dissolving them completely to obtain a mixture D; Step S2-3, cooling the mixture C to 45° C., adding the mixture D thereto, and stirring evenly to obtain a mixture CD; Step S2-4, cooling the mixture CD to 40°C, adding the components in A, B, E and the remaining deionized water, stirring evenly at 200 r / min, and obtaining a firming and anti-wrinkle facial cream.

[0042] Table 3 Mass percentage of raw materials for firming and anti-wrinkle cream

[0043] Allergy test The above Examples 1-3 were subjected to allergy testing with reference to the human skin patch test in the "2015 Cosmetic Safety Technical Specifications".

[0044] The method of skin occlusion patch test is: select 90 people aged 18-60 years old, and select an area not exceeding 50mm 2 , a qualified patch test device with a depth of about 1mm, take 0.020mL of the finished product prepared in the above embodiment and put it into the patch test chamber, the control well is a blank control (no substance is placed), and the patch tester with the finished product is applied to the curved side of the forearm of the subject with a low-allergenic tape, and the palm is gently pressed to make it evenly applied to the skin for 24 hours. The skin reaction is observed 30 minutes after the removal of the finished product (after the indentation disappears), 24 hours and 48 hours, and the observation results are recorded.

[0045] The reaction level of all subjects was "-", grade 0 reaction; that is, negative reaction, no allergic reaction occurred.

[0046] Human efficacy experiment 1. Subject Screening There were 15 subjects in total, aged 25-30 years old, female, with little difference in skin conditions, with an average of 5 people in each group, and each group corresponded to test examples 1-3.

[0047] 2. Rules for use of test products The subjects used the product once a day, morning and evening, and evenly applied the essence on the face until it was completely absorbed. During the test period, the subjects were required to stop using other similar products. The test lasted for 8 consecutive weeks, and the subjects were revisited once at the 0th, 4th, and 8th weeks, for a total of 3 times.

[0048] On the day of the test, the subjects were required to clean their faces with the cleansing products provided by the laboratory and sit quietly for 30 minutes. The researchers then tested the skin on both sides of the cheeks. The entire test was conducted in a constant temperature environment of (21.0±1.0)℃ and relative humidity of 50%±10%.

[0049] 3. Test Methods The elastic parameter R2 of the cheek was measured using the 2mm probe of Cutometer® MPA 580. R2 = Ua / Uf, which represents the ratio of the skin's rebound without negative pressure to the maximum stretch with negative pressure. It is considered to be the parameter value closest to the true state of skin elasticity. The closer the ratio is to 1, the better the skin elasticity. The average value of the five measurements is taken, and the results are shown in Table 4.

[0050] 4. Test Results Table 4 Skin elasticity R2 value

[0051] 5. Results Analysis According to the results of Examples 1-3, the greater the concentration of the composition, the greater the change in the skin elasticity R2 value, indicating that the composition has excellent firming and anti-wrinkle effects and has a certain concentration dependence; the firming and anti-wrinkle cream provided by the present invention can effectively improve the elasticity value of the skin, that is, the cream can significantly improve the firmness of the skin and has a significant anti-wrinkle effect.

[0052] Finally, it should be noted that the above embodiments and comparative examples are only used to illustrate the technical solution of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A firming and anti-wrinkle composition, characterized in that: The composition comprises the following components in parts by mass: 2-5 parts of alpine edelweiss flower / leaf extract; 1-6 parts of peach resin extract; Gentiana root extract 0.5-0.7 parts; 1-4 parts of nasturtium flower extract.

2. The firming and anti-wrinkle composition according to claim 1, characterized in that: The preparation method of the nasturtium flower extract comprises the following steps: S1-1. Take nasturtium and crush, sieve, and obtain nasturtium powder; S1-2. Deionized water was added to the nasturtium powder, ultrasonically treated for 20-30min, and the filtrate A and the residue A were filtered to obtain a standby; S1-3. Add ≥95 v / v% ethanol aqueous solution to the residue A, reflux and extract, and filter to obtain filtrate B; S1-4. After combining filtrate A and filtrate B, the filtrate is concentrated and dried to obtain nasturtium flower extract.

3. The firming and anti-wrinkle composition according to claim 2, characterized in that: The mesh size of the sieving in step S1-1 is 60 meshes.

4. The firming and anti-wrinkle composition according to claim 2, characterized in that: In the step S1-2, the mass ratio of nasturtium powder to deionized water is 1:10, and the frequency of ultrasound is 28 kHz.

5. The firming and anti-wrinkle composition according to claim 2, characterized in that: In the step S1-3, the amount of ethanol aqueous solution added is 5 times the mass of the filter residue, the temperature of reflux extraction is controlled at 85°C, the number of refluxes is 3 times, and each reflux is 0.7h.

6. Use of the firming and anti-wrinkle composition according to any one of claims 1 to 5 in the preparation of toners, essences, gels, emulsions and / or creams having firming and anti-wrinkle effects.

7. A facial cream with firming and anti-wrinkle effects, characterized in that: The invention comprises the firming and anti-wrinkle composition according to any one of claims 1 to 5, wherein the amount of the firming and anti-wrinkle composition added to the facial cream is 1-3wt%.

8. The facial cream with firming and anti-wrinkle effects according to claim 7, characterized in that: The facial cream with firming and anti-wrinkle effects also contains composite polypeptides, recombinant collagen, skin conditioners, thickeners, emulsifiers, emollients, preservatives, chelating agents, moisturizers, and solvents.

9. The facial cream with firming and anti-wrinkle effects according to claim 8, characterized in that: The composite polypeptide is at least two of palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, acetyl hexapeptide-8, dipeptide diaminobutyryl benzylamide diacetate, carnosine, and nonapeptide-1; The recombinant collagen is at least one of recombinant type XVII collagen, recombinant type I collagen, recombinant type IV collagen, recombinant type V collagen, and recombinant type VII collagen; The skin conditioning agent is at least one of polydeoxyribonucleotide, nicotinamide, caprylhydroxamic acid, and 3-o-ethyl ascorbic acid; The thickener is at least one of sodium polyacrylate, acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, xanthan gum, carbomer, and polyacrylamide; The emulsifier is at least one of PEG-20 methyl glucose sesquistearate, glyceryl stearate, sorbitan stearate, PEG-100 stearate, and cetearyl glucoside; The emollient is at least one of dicaprylyl carbonate, cetearyl alcohol, hydrogenated polyisobutylene phenyl trimethicone, tocopheryl acetate, C13-14 isoparaffin, jojoba seed oil, squalane, caprylic / capric triglyceride, ethylhexyl palmitate, avocado butter, dimethicone, lavender oil, grapefruit peel oil, ylang-ylang flower oil, lemon peel oil, and damascena flower oil; The preservative is at least one of p-hydroxyacetophenone, methylparaben, caprylhydroxamic acid, glyceryl caprylate, 1,2-pentanediol, phenoxyethanol, ethylhexylglycerin, and caprylyl glycol; The chelating agent is at least one of disodium EDTA and tetrahydroxypropylethylenediamine; The moisturizing agent is at least one of β-glucan, glycerol, sodium polyglutamate, Tremella fuciformis fruiting body extract, sodium hyaluronate, allantoin, butylene glycol, propylene glycol, and 1,2-hexanediol; The solvent is deionized water.

10. The method for preparing the facial cream with firming and anti-wrinkle effects according to claim 9, characterized in that: The preparation method comprises the following steps: The firming and anti-wrinkle composition, composite polypeptide, recombinant collagen, skin conditioner, thickener, emulsifier, moisturizer, preservative, chelating agent, moisturizer and solvent are uniformly mixed to obtain the firming and anti-wrinkle face cream.

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