Regulation and control and use for differentiation by neuro stem cells

A technology of neural stem cells and genes, applied in the fields of bioengineering and developmental biology, can solve problems such as difficulties in in vitro isolation and culture

Inactive Publication Date: 2005-11-09
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the inherent difficulty in the isolation and culture of neural stem cells in vitro, the current reports on them are often limited to the isolation and culture techniques.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0007] Materials and Methods

[0008] 1. Isolation, culture and identification of neural stem cells

[0009] 1. Subculture of neural stem cells (cultivation of undifferentiated neural stem cells)

[0010] Primary culture (culture medium is basic medium + 20ng·mL -1 After one week of EGF and bFGF), the cell mass was collected, blown into a single-cell suspension with a pipette, and then the cell suspension was collected into a 50mL centrifuge tube with a pipette, 1500rpm, 5 minutes, the supernatant was discarded, and then used Resuspend the cell pellet in fresh medium and transfer to a sterile culture flask with a pipette.

[0011] The observation results found that the cells were observed as round bright spots on the first day of the primary culture process; on the second and third days, a few cells formed a dumbbell-shaped shape, most of the individual cells had adhered to the wall, and some cells had protrusions ;On the 4th and 5th days, suspension balls of about dozens o...

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PUM

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Abstract

A gene NM-017084.1 with obviously different expression in the nerve stem cell differentiating procedure is disclosed. It can promote the differentiation of nerve steam cell after its expression is silenced by RNAi.

Description

field of invention [0001] The invention belongs to bioengineering and developmental biology, and relates to the genetic engineering of genes related to the differentiation and development of neural stem cells, and the use of technologies such as RNA interference to use genes related to the differentiation and development of neural stem cells and their products as targets for intervening in the differentiation and development of stem cells, thereby regulating neural stem cells differentiation and development. Background of the invention [0002] Neural stem cells refer to regenerative cells that have the ability to differentiate into neurons, astrocytes, and oligodendrocytes, and can self-renew, enough to provide brain tissue cells. In the development of the mammalian central nervous system, maintaining the function of the central nervous system plays an important role. The differentiation of neural stem cells is an extremely complex process. There are many factors that aff...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12
Inventor 文铁桥李海龙
Owner SHANGHAI UNIV
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