Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Regulation and control and use for differentiation by neuro stem cells

A technology of neural stem cells and genes, applied in the fields of bioengineering and developmental biology, can solve problems such as difficulties in in vitro isolation and culture

Inactive Publication Date: 2005-11-09
SHANGHAI UNIV
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the inherent difficulty in the isolation and culture of neural stem cells in vitro, the current reports on them are often limited to the isolation and culture techniques.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0007] Materials and Methods

[0008] 1. Isolation, culture and identification of neural stem cells

[0009] 1. Subculture of neural stem cells (cultivation of undifferentiated neural stem cells)

[0010] Primary culture (culture medium is basic medium + 20ng·mL -1 After one week of EGF and bFGF), the cell mass was collected, blown into a single-cell suspension with a pipette, and then the cell suspension was collected into a 50mL centrifuge tube with a pipette, 1500rpm, 5 minutes, the supernatant was discarded, and then used Resuspend the cell pellet in fresh medium and transfer to a sterile culture flask with a pipette.

[0011] The observation results found that the cells were observed as round bright spots on the first day of the primary culture process; on the second and third days, a few cells formed a dumbbell-shaped shape, most of the individual cells had adhered to the wall, and some cells had protrusions ;On the 4th and 5th days, suspension balls of about dozens o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A gene NM-017084.1 with obviously different expression in the nerve stem cell differentiating procedure is disclosed. It can promote the differentiation of nerve steam cell after its expression is silenced by RNAi.

Description

field of invention [0001] The invention belongs to bioengineering and developmental biology, and relates to the genetic engineering of genes related to the differentiation and development of neural stem cells, and the use of technologies such as RNA interference to use genes related to the differentiation and development of neural stem cells and their products as targets for intervening in the differentiation and development of stem cells, thereby regulating neural stem cells differentiation and development. Background of the invention [0002] Neural stem cells refer to regenerative cells that have the ability to differentiate into neurons, astrocytes, and oligodendrocytes, and can self-renew, enough to provide brain tissue cells. In the development of the mammalian central nervous system, maintaining the function of the central nervous system plays an important role. The differentiation of neural stem cells is an extremely complex process. There are many factors that aff...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12
Inventor 文铁桥李海龙
Owner SHANGHAI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com