Bioreactor system for nerve stem cell to amplify in large scale and long time

Abstract

The invention discloses a bioreactor system of nerve stem cell's long-term expanding in big scale for bio-engineering technology field. This invention including: the air feed unit, the unit of nutrient fluid providing, the unit of cell expanding and increasing and as soon as wriggles pumping, its connection way is: The air feed unit terminal expands through the silica gel air conduit with the cell increases unit connected, the nutrient fluid supplies unit's terminal expands through the silica gel infusion tube with the cell increases unit connected, as well as wriggles pump in the silica gel infusion. This invention get out of the limit of raising box, and has advantageous for a long time greatly expanding and increasing scale and synchronous detect, and provides most suitable environment for nerve stem cell's raising in high density and long-term rasing.

Description

technical field [0001] The invention relates to a bioreactor system, in particular to a bioreactor system for long-term large-scale expansion of neural stem cells. Used in the field of bioengineering technology. Background technique [0002] In 1992, Reynold and Weiss isolated neural stem cells from the central nervous system of adult mice for the first time, and successfully expanded them in vitro, marking that the research on neural stem cells has entered a new stage. Since nerve cells are not specialized antigen-presenting cells (APCs), the immune response to the transplanted allogeneic nerve tissue is not as strong as that of other tissues, so the allogeneic neural stem cells expanded in vitro will get rid of this restriction factor during transplantation and become a therapeutic agent. A powerful tool for neurological diseases. Now neural stem cells have been tried to be used in the treatment of central nervous diseases, such as Parkinson's syndrome, multiple sclerosi...

AI Technical Summary

Problems solved by technology

The device cannot flexibly control the O 2 , CO 2 The supply of gas, etc., cannot continuously provide medium for the reactor and remove metabolic by-products at the same time, so that the optimal growth environment for neural stem cells cannot be maintained

Moreover, there is a risk of bacterial contamination during the inoculation of neural stem cells

In addition, since the entire culture process is carried out in an incubator, it is extremely inconvenient for observation and sampling, and the inherent volume of the incubator limits the scale of neural stem cell expansion to a certain extent.

All in all, the design of this system is too limited and not conducive to operation and practical application, that is, it cannot get rid of the limitation of the incubator, it is not conducive to expanding the scale of amplification, and it cannot be detected simultaneously; the design lacks flexibility and cannot be used for long-term culture of neural stem cells. Provide the most suitable environment

Therefore, using this bioreactor system to expand neural stem cells will not only consume the time and energy of researchers, but more importantly, such devices cannot achieve long-term continuous high-density culture of neural stem cells

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