Medicine for treating nerves damage and preparation method thereof

A technology for the treatment of drugs and nerve damage, applied in the field of biomedicine, can solve the problems of lack of living environment, limited curative effect and poor curative effect at the damaged site

Inactive Publication Date: 2008-11-19
奔驰生物科技(云南)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, both at home and abroad, in the treatment of nerve damage, there are reports on the use of single nerve growth factor NGF or neurotrophic factor NTF drugs, and oral (Xiong Yuliang et al.: The preparation method of medicines for the treatment of peripheral nervous system diseases and male reproductive defects) , Patent Application No. 00116192.X, published on July 25, 2001), there is also the use of intramuscular injection, although it has a certain effect, but the curative effect is poor
The reason is that the drugs corresponding to these conventional administration methods, nerve growth factor NGF, decay faster in the bo

Method used

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  • Medicine for treating nerves damage and preparation method thereof
  • Medicine for treating nerves damage and preparation method thereof
  • Medicine for treating nerves damage and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Fresh spinal cord within 30 minutes from human embryos was washed repeatedly with 1640 or 199 culture medium, cut into ganglia of about 1 mm, cultured in a 37°C incubator for 48 hours, and cobra venom was added for nerve growth After 8 days of co-cultivation of factor NGF and culture medium, the culture medium is a mixed culture medium of 1640 or 199 and 15% newborn calf serum, which accounts for 85% by weight, and is additionally added with penicillin 100 units / ml and streptomycin 100 μg / ml , The culture medium is maintained at pH 7.0. Finally, the cell content in the co-cultured combination drug is not less than 400×10 4 Pieces / ml~600×10 4 Pieces / ml, the content of living cells is not less than 85%, and the NGF is not less than 2500 to 5000 units. Frozen storage and passage. The cryopreserved cells were resuscitated before the operation and implanted in the pia mater of the spinal cord at the injury site.

Embodiment 2

[0021] Example 2: A fresh spinal cord was removed from the body of a golden monkey, washed repeatedly with 1640 or 199 culture medium within 30 minutes, cut into ganglia of about 1 mm, cultured in a 37°C incubator for 48 hours, and added to the mouse submandibular gland After 8 days of co-cultivation of nerve growth factor NGF and culture medium, the culture medium is a mixed culture medium of 1640 or 199 and 15% newborn calf serum, which accounts for 85% by weight, and is additionally added with penicillin 100 units / ml and streptomycin 100 μg / ml, the culture medium is maintained at pH 7.0. Finally, the cell content in the co-cultured combination drug is not less than 400×10 4 Pieces / ml~600×10 4 Pieces / ml, the content of living cells is not less than 85%, and the NGF is not less than 2500 to 5000 units. Frozen storage and passage. The cryopreserved cells were resuscitated before surgery and implanted into the pia mater of the spinal cord of the golden monkey. The measurement resu...

Embodiment 3

[0022] Example 3: Remove the fresh spinal cord from the mouse body, wash it repeatedly with 1640 or 199 culture medium within 30 minutes, cut it into ganglia of about 1 mm, culture it in a 37°C incubator for 48 hours, add the cobra snake venom nerve After the growth factor NGF and the culture solution were co-cultured for 8 days, the culture solution was a mixed culture solution of 85% by weight of 1640 or 199 and 15% of newborn calf serum, and added penicillin 100 units / ml and streptomycin 100 μg / ml, the culture medium is kept at pH 7.0. Finally, the cell content in the co-cultured combination drug is not less than 400×10 4 Pieces / ml~600×10 4 Pieces / ml, the content of living cells is not less than 85%, and the NGF is not less than 2500 to 5000 units. Frozen storage and passage. The cryopreserved cells were resuscitated before the operation and implanted around the sciatic nerve injured by the clamp. The results of the pain recovery measurement of the mice are shown in Table 2.

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Abstract

The invention provides a medicine used for curing nerve damage and a method used for preparing the medicine. The invention belongs to the technical field of a biologic medicine. The medicine is prepared with snake venom nerve growth factors NGF, neurotrophy factors NTF, nerve stem cells and Schwann cells according to the following method: the entire spinal cord of a newborn animal or a fetus which can not be conceived because of natural and man-made calamities is implanted in aseptic PBS liquid or culture fluid; required ganglia are cleaned and shorn again and again. After the required ganglia are cultured in a thermostat of 37 DEG C for 48 hours, little culture fluid is added; the required ganglia are cultured for eight days before the required ganglia are frozen and deposited for passage. Before operation, the frozen and deposited cells are revived, and are implanted in the pia mater spinalis of the damaged spinal cord. The medicine can provide the cultured cells and the damaged neurocyte with new nutriments and NGF required for promoting the growth. The implanted cells can be separated and added to the damaged neural parts to repair the damaged, dead or feeble cells with safe and remarkable cure effect.

Description

Technical field [0001] The invention relates to a nerve injury treatment medicine and a preparation method thereof, and belongs to the technical field of biomedicine. Background technique [0002] At present, in the treatment of nerve injury at home and abroad, there are reports on the use of single nerve growth factor NGF or neurotrophic factor NTF drugs, and oral (Xiong Yuliang et al.: Preparation method of drugs for the treatment of peripheral nervous system diseases and male reproductive defects , Patent Application No. 00116192.X, published on July 25, 2001), there are also intramuscular injections, although it has a certain effect, but the effect is poor. The reason is that the drugs corresponding to these conventional methods of administration, the nerve growth factor NGF decays faster in the body, and the dose at the nerve injury site is already small, so the efficacy is limited, especially the efficacy of oral drugs is even worse. . In order to make the injured site have...

Claims

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Application Information

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IPC IPC(8): A61K38/18A61P25/00A61K31/43A61K31/7036
Inventor 熊郁良王婉瑜吕秋敏吴健波朱绍文李瑞
Owner 奔驰生物科技(云南)有限公司
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