Medicine for treating nerves damage and preparation method thereof

Abstract

The invention provides a medicine used for curing nerve damage and a method used for preparing the medicine. The invention belongs to the technical field of a biologic medicine. The medicine is prepared with snake venom nerve growth factors NGF, neurotrophy factors NTF, nerve stem cells and Schwann cells according to the following method: the entire spinal cord of a newborn animal or a fetus which can not be conceived because of natural and man-made calamities is implanted in aseptic PBS liquid or culture fluid; required ganglia are cleaned and shorn again and again. After the required ganglia are cultured in a thermostat of 37 DEG C for 48 hours, little culture fluid is added; the required ganglia are cultured for eight days before the required ganglia are frozen and deposited for passage. Before operation, the frozen and deposited cells are revived, and are implanted in the pia mater spinalis of the damaged spinal cord. The medicine can provide the cultured cells and the damaged neurocyte with new nutriments and NGF required for promoting the growth. The implanted cells can be separated and added to the damaged neural parts to repair the damaged, dead or feeble cells with safe and remarkable cure effect.

Description

Technical field [0001] The invention relates to a nerve injury treatment medicine and a preparation method thereof, and belongs to the technical field of biomedicine. Background technique [0002] At present, in the treatment of nerve injury at home and abroad, there are reports on the use of single nerve growth factor NGF or neurotrophic factor NTF drugs, and oral (Xiong Yuliang et al.: Preparation method of drugs for the treatment of peripheral nervous system diseases and male reproductive defects , Patent Application No. 00116192.X, published on July 25, 2001), there are also intramuscular injections, although it has a certain effect, but the effect is poor. The reason is that the drugs corresponding to these conventional methods of administration, the nerve growth factor NGF decays faster in the body, and the dose at the nerve injury site is already small, so the efficacy is limited, especially the efficacy of oral drugs is even worse. . In order to make the injured site have...

AI Technical Summary

Problems solved by technology

[0002] At present, both at home and abroad, in the treatment of nerve damage, there are reports on the use of single nerve growth factor NGF or neurotrophic factor NTF drugs, and oral (Xiong Yuliang et al.: The preparation method of medicines for the treatment of peripheral nervous system diseases and male reproductive defects) , Patent Application No. 00116192.X, published on July 25, 2001), there is also the use of intramuscular injection, although it has a certain effect, but the curative effect is poor

The reason is that the drugs corresponding to these conventional administration methods, nerve growth factor NGF, decay faster in the body, and the dosage at the nerve injury site is already very small, so the curative effect is limited, especially the curative effect of oral drugs is even worse

In order to make the damaged site have a higher level of NGF, domestic and foreign studies and tests have used a single implant of NGF. Due to the lack of a living environment in the damaged site, the lack of factors such as NGF and NTF that promote the growth and differentiation of nerve cells and the growth of nerve fibers, resulting in implantation Premature apoptosis of entering cells makes it difficult for the nerve cells in the damaged area to restore growth and differentiation

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