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Application of components in promoting in-vitro proliferation of neural stem cells and further in neural stem cell transplantation

A neural stem cell, in vitro proliferation technology, applied in the direction of nervous system cells, animal cells, vertebrate cells, etc., can solve the problem of no isonaringoside neural stem cells

Inactive Publication Date: 2021-02-26
南京温博生物科技有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] Isohemiphloin (Isohemiphloin) is a glycoside compound, and there is currently no report that isohemiphloin is used to promote the proliferation of neural stem cells in vitro

Method used

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  • Application of components in promoting in-vitro proliferation of neural stem cells and further in neural stem cell transplantation
  • Application of components in promoting in-vitro proliferation of neural stem cells and further in neural stem cell transplantation
  • Application of components in promoting in-vitro proliferation of neural stem cells and further in neural stem cell transplantation

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Embodiment Construction

[0014] The substantive content of the present invention will be described below in conjunction with specific embodiments, but the protection scope of the present invention cannot be limited thereto.

[0015] 1. Experimental materials

[0016] SPF-grade female SD rats, 15-17 days pregnant, were purchased from Changzhou Cavens Experimental Animal Co., Ltd.

[0017] Isonaringoglucoside was purchased from Sichuan Weikeqi Biotechnology Co., Ltd., with HPLC purity ≥ 98%.

[0018] Trypsin, fetal bovine serum, B27, bFGF, penicillin and streptomycin and DMEM / F12 medium were purchased from Beyontien Biotech.

[0019] 2. Experimental method

[0020] 1. Primary culture and passage of NSCs

[0021] After the pregnant mice were anesthetized with 10% chloral hydrate intramuscular injection, the fetal mice were taken, the brains were taken under aseptic conditions, the meninges were peeled off, the hippocampus tissue was separated, cut into pieces, digested with 0.05% trypsin, and added to...

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Abstract

The invention discloses application of components in promoting in-vitro proliferation of neural stem cells and further in neural stem cell transplantation. The Neural Stem Cells (NSCs) as the most promising cell type in stem cell treatment of neurological diseases have broad treatment prospects in treatment of Parkinson's disease, spinal cord injury, cerebral apoplexy and other diseases. ExogenousNSCs transplantation has high requirements for the number of neural stem cells. However, if only relying on NSCs conventional culture, the speed is limited. The invention finds that isonaringin has the activity of promoting in-vitro proliferation of NSCs, and the high concentration isonaringin has stronger promotion effect on the in-vitro proliferation activity of NSCs.

Description

technical field [0001] The invention belongs to the field of stem cells, and relates to the application of a component in promoting the proliferation of neural stem cells in vitro and then being used for neural stem cell transplantation. Background technique [0002] Neural stem cells (Neural stem cells, NSCs) are a kind of tissue-specific stem cells, which are derived from the neuroepithelium developmentally and have the potential of self-renewal and directed differentiation into neural cells. As the most promising cell type in the treatment of neurological diseases with stem cells, the current basic and clinical researches are widely focused on the treatment of Parkinson's, spinal cord injury, stroke and other diseases. Due to the influence of the number and the local microenvironment after injury, the possibility of relying solely on the self-proliferation and repair of such cells is very small. Therefore, the use of exogenous neural stem cell transplantation to act on t...

Claims

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Application Information

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IPC IPC(8): C12N5/0797
CPCC12N5/0623C12N2501/999
Inventor 姜东花
Owner 南京温博生物科技有限公司
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