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Laver free filament factory cultivation method based on function foodstuff development

A cultivation method and a technology of functional food, which are applied in the field of industrial cultivation of laver free filaments, can solve the problems of being unable to develop food-grade phycobiliproteins, consume a lot of manpower and material resources, and limit the scope of application, and achieve the goal of improving phycobiliproteins. Protein content, convenient source, and the effect of ensuring biomass

Inactive Publication Date: 2015-12-09
SHANTOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Patent 201010299370.4 "A kind of Porphyra filamentous algae with high content of phycoerythrin obtained by optimizing and regulating culture conditions" and 201010238278.4 "A method of optimizing and regulating culture conditions to improve the growth rate of Porphyra filamentous algae" achieved by optimizing and regulating culture conditions To improve the phycobiliprotein content and production rate of Porphyra, they use mature thallus to extract fruit spores, and then cultivate them into filaments. This method needs to extract fruit spores after picking vegetables every year. The process of purchasing, storing and picking fruit spores requires a lot of manpower and material resources. In addition, vegetables can only be produced once a year, which has obvious seasonal restrictions.
Moreover, the nutrient solution formula of patent 201010299370.4 "A Porphyra Porphyra filamentous algae with high content of phycoerythrin obtained by optimizing and regulating culture conditions" contains heavy metal copper plasma that inhibits the growth and pollution of macroalgae, and cannot be used to develop food-grade phycobilis protein, which limits the scope of application

Method used

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  • Laver free filament factory cultivation method based on function foodstuff development
  • Laver free filament factory cultivation method based on function foodstuff development
  • Laver free filament factory cultivation method based on function foodstuff development

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Embodiment 1

[0037] The present invention is a kind of factory cultivation method of laver free filament developed based on functional food, and the cultivation method process is as follows: figure 1 As shown, this embodiment specifically includes the following steps.

[0038] S1 material selection: the free filamentous strain of algae Porphyra (filamentous algal filaments > 99%) selected by the Algae Breeding Room of the Key Laboratory of Marine Biology, Shantou University. The fronds were collected in Pingyu, Nanao, Shantou in 2012. Completely isolated from the mainland and less polluted. The free filaments were crushed with a pulverizer for 15 seconds, the algal bodies were sieved and washed with sterilized seawater, placed in a 20°C culture room (±1°C) for temporary cultivation, and the light was 40μmol / (m 2 ·s), the light-to-dark ratio is 12h:12h, and the medium 3 / 5 is replaced every seven days. Wait until 15 days for use.

[0039] S2 algae culture: three levels of culture are set ...

Embodiment 2

[0050] S1 material selection: the free filamentous strain of algae Porphyra (filamentous algal filaments > 99%) selected by the Algae Breeding Room of the Key Laboratory of Marine Biology, Shantou University. The fronds were collected in Pingyu, Nanao, Shantou in 2012. Completely isolated from the mainland and less polluted. The free filaments were crushed with a pulverizer for 15 seconds, the algae were filtered with sieved silk, washed with sterilized seawater, placed in a 20°C culture room (±1°C) for temporary cultivation, and the light was 40μmol / (m 2 ·s), the light-to-dark ratio is 12h:12h, and the medium 3 / 5 is replaced every seven days. Wait for up to 14 days for use;

[0051] S2 algal filament culture: Select the above-mentioned filaments with good culture status and inoculate them into conical flasks with an initial density of 1g: 1L culture solution, place them in a 20°C culture room (±1°C) for suspension culture, and set the speed according to the size of the cultu...

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Abstract

The invention relates to an algae cultivation method, and specifically relates to a laver free filament factory cultivation method based on function foodstuff development; the method comprises the following steps: s1, material selection: selecting laver free filaments with good growth state, mixing the laver free filaments with a nutrient solution in proportion, using a pulverizer to break and cut the laver free filaments, bolting and filtering the algae and using disinfection seawater to wash, adding fresh nutrient solution and placing the algae under specific parameters for cultivation, replacing the nutrient solution in every 7days until wound heals for 14-15days, and preparing; s2, algae filament cultivation: selecting the filaments cultivated in the s1, inoculating the filaments in a culture container according to initial density 0.8-1.2g: 1L nutrient solution, placing the nutrient solution in specific parameters for cultivation, and replacing the nutrient solution in every 7days. Compared with the prior art, the laver free filament factory cultivation method based on function foodstuff development can keep a high growth rate of the laver free filament and improve phycobiliprotein content in the laver free filaments, thus satisfying development function food stuff requirements.

Description

technical field [0001] The invention relates to a method for cultivating algae, in particular to a method for industrialized cultivation of laver free filaments developed based on functional food. Background technique [0002] Porphyra is a kind of large marine algae with edible value and economic value. Our annual production of laver is huge, which is particularly important for the development and research of laver. At present, the research of domestic and foreign scholars mainly focuses on two aspects. One is the cultivation of laver, which mainly studies the growth law and growth environment of laver, and optimizes the growth conditions of cultivated laver. However, due to the limitations of local sea areas and seasons, the cultivation scale and When the quantity reaches a certain level, it is difficult to increase it. Researchers have been exploring industrialized and large-scale laver cultivation methods. The second is research on the functional development of laver. T...

Claims

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Application Information

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IPC IPC(8): A01G33/02
Inventor 黄冰心颜泽伟丁兰平张全亮谭华强马元元李雯诗徐佩杭曾令昭胡凯
Owner SHANTOU UNIV
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