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Method of extracting bio-active substances from algae

A technology of bioactive substances and algae, which is applied in the preparation method of peptides, chemical instruments and methods, organic chemistry, etc., can solve the problems of limited output, single extraction of bioactive ingredients, and scarce distribution of Gexianmi, so as to save raw materials, High yield and purity, guaranteed yield effect

Active Publication Date: 2016-01-13
神农架天然美妆原料研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although there are occasional reports about single extraction of certain biologically active ingredients at present, there are no reports of extracting multiple biologically active ingredients at the same time, especially for Gexianmi, because the distribution of Gexianmi in the world is very rare, the output It is also limited, and it is of great significance to develop methods for efficiently extracting (multiple) biologically active substances from algae to provide a basis for the study of biochemical nature

Method used

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Examples

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Embodiment 1

[0062] (1) Extraction by breaking the wall: Weigh 20g of Ge Xianmi sample, swell with 200mL of pH 7.3 phosphate buffer (0.02mol / L) at 4°C, freeze at -20°C for 8h, and thaw at 25°C, repeat the process for 3 The second time, add 400mL of extraction buffer, place in a high-speed tissue homogenizer for high-speed tissue homogenization (5000rpm, 4°C for 4h), centrifuge at 10000r / min for 20min at 4°C, and take the supernatant.

[0063] (2) Salting out: add ammonium sulfate to the supernatant (so that the concentration of ammonium sulfate in the system is 20% by weight), precipitate at 4°C for 8h, centrifuge at 10000r / min for 20min at 4°C, take the supernatant, discard After precipitation, the supernatant was continued to be precipitated with ammonium sulfate (the concentration of ammonium sulfate in the control system was 30% by weight) (4°C, 8h), centrifuged at 10000r / min for 20min at 4°C, and the supernatant was taken by centrifugation and the precipitate was collected. In this wa...

Embodiment 2

[0067] (1) Extraction by breaking the wall: Weigh 20 g of Ge Xianmi sample, swell with 80 mL of pH 5 phosphate buffer (0.02mol / L) at 15°C, freeze at -20°C for 8 hours, and thaw at 25°C, and repeat the treatment 3 times. Add 400mL of extraction buffer, place in a high-speed tissue homogenizer for high-speed tissue homogenization (5000rpm, 15°C for 4h), centrifuge at 10000r / min for 20min at 4°C, and take the supernatant.

[0068] (2) Salting out: add ammonium sulfate to the supernatant (so that the concentration of ammonium sulfate in the system is 15% by weight), precipitate at 4°C for 8h, centrifuge at 10000r / min for 20min at 4°C, take the supernatant, discard After precipitation, the supernatant was continued to be precipitated (4°C, 8h) with ammonium sulfate (the concentration of ammonium sulfate in the control system was 35% by weight), centrifuged at 10000r / min for 20min at 4°C, and the supernatant was taken by centrifugation and the precipitate was collected. In this way,...

Embodiment 3

[0072] (1) Extraction by breaking the wall: Weigh 20g of Ge Xianmi sample, swell with 300mL of pH 8.5 phosphate buffer solution (0.02mol / L) at 10°C, freeze at -20°C for 8h, and thaw at 25°C, repeat the process for 3 The second time, add 400mL of extraction buffer, place in a high-speed tissue homogenizer for high-speed tissue homogenization (5000rpm, 10°C for 4h), centrifuge at 10000r / min for 20min at 4°C, and take the supernatant.

[0073] (2) Salting out: add ammonium sulfate to the supernatant (so that the concentration of ammonium sulfate in the system is 25% by weight), precipitate at 4°C for 8h, centrifuge at 10000r / min for 20min at 4°C, take the supernatant, discard After precipitation, the supernatant was continued to be precipitated (4°C, 8h) with ammonium sulfate (the concentration of ammonium sulfate in the control system was 50% by weight), centrifuged at 10000r / min for 20min at 4°C, and the supernatant was obtained by centrifugation and the precipitate was collecte...

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Abstract

The invention relates to the field of deep processing of algae and discloses a method of extracting bio-active substances from algae. The method includes the following steps: 1) performing cell disruption to an algae sample to dissolve-out phycobiliprotein, and performing solid-liquid separation to obtain a crude extract liquid; 2) mixing the crude extract liquid with inorganic salts to perform salting out, wherein the concentration of the inorganic salts is 15-25% by weight in the mixture system, and performing solid-liquid separation to obtain a liquid phase; 3) selectively mixing the liquid phase with inorganic salts to perform salting out, wherein the concentration of the inorganic salts is 30-55% by weight in the mixture system, and performing solid-liquid separation to collect precipitates; and 4) mixing the liquid phase obtain through the solid-liquid separation in the step 2) or 3) with inorganic salts to perform salting out, wherein the concentration of the inorganic salts is 58-65% by weight in the mixture system, and performing solid-liquid separation to collect precipitates. The method also can comprise a step of extracting polysaccharides. The method can extract the bio-active substances from algae at a high efficiency.

Description

technical field [0001] The invention relates to a method for extracting biologically active substances from algae, in particular to a method for extracting phycobiliprotein and / or polysaccharide from algae. Background technique [0002] Algae can not only be used as food, but also have a close relationship with medicine and agriculture. Taking Nostocsphaeroidskütz as an example, Nostocsphaeroidskütz is an edible cyanobacterium born in rice fields, belonging to the genus Nostoc in the phylum Cyanophyta (Cyanophyceae) and the family Nostocaceae. , also known as tianxiancai, tianxianmi, also known as water fungus, is a freshwater cyanobacteria, which is a traditional and precious wild algae for both food and medicine in my country. The main distribution area in my country is Zoumaping Town, Hefeng County, Hubei Province. Gexianmi has the effects of improving eyesight, nourishing qi, curing night blindness, prolonging life after long-term food, relieving heat and clearing diaph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/30C07K1/14C08B37/00
Inventor 莫开菊程超汪兴平
Owner 神农架天然美妆原料研究院
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