107 results about "Brassinine" patented technology

The invention belongs to the technical field of plant growth conditioning agents, and relates to a plant salt resisting conditioning agent. The plant salt resisting conditioning agent is prepared from porphyra polysaccharides with the concentration of 100-1,000 mg / L, salicylic acid with the concentration of 0 mg / L or 100-200 mg / L, brassinolide with the concentration of 0 mg / L or 0.01-0.1 mg / L, glycine betaine with the concentration of 0 mg / L or 100-1,000 mg / L, spermidine with the concentration of 0 mg / L or 10-100 mg / L, a surfactant with the concentration of 0 mg / L or 1,000-20,000 mg / L and water, wherein the water serves as a solvent, and low-molecular-weight porphyra polysaccharides with the molecular weight of 1 k-50 k Da are adopted as the porphyra polysaccharides. Accordingly, novel application of the porphyra polysaccharides is supplied, and the porphyra polysaccharides can be used for preparing the plant salt resisting conditioning agent; meanwhile, by compounding the porphyra polysaccharides with the biological source induced resisting substances such as salicylic acid, brassinolide, glycine betaine and spermidine to prepare the salt resisting conditioning agent, the comprehensive resistance of plants on salt stress can be improved, and normal growth and development of the plants are guaranteed; according to the salt resisting conditioning agent, the components are simple, the raw materials are easy to obtain, the cost is low, the effect is significant, and environmental friendliness is achieved.

The invention discloses a preparation for preventing and controlling crop bacterial wilt and a preparation method thereof. The preparation consists of the following raw materials: brassinolide, magnesium sulfate, ferrous sulfate, boric acid, compound amino acid, alcohol and water. The preparation method comprises the following steps of: dissolving the brassinolide into the alcohol; adding ethyoxyl trisiloxane; mixing uniformly to obtain a solution; mixing the obtained solution with the magnesium sulfate, ferrous sulfate, boric acid and compound amino acid; and adding water for fixing a volume to obtain the preparation for preventing and controlling crop bacterial wilt. The preparation for preventing and controlling crop bacterial wilt can be used for promoting the synthesis of reduced glutathione, increasing the activity of resistance enzymes, activating the expression of resistance genes, enhancing the resistance of crops, preventing bacterial from invading crops, effectively suppressing the growth of ralstonia solanacearum, remarkably relieving the mobidity extent of bacterial wilt, relieving the bacterial wilt of plants by over 54 percent, and fundamentally reducing economic loss caused by crop morbidity.

The invention relates to a compound plant ash dendrobium seedling culture medium material and a preparation method and application thereof. The preparation method comprises the steps of: mixing plant ash with plant hormone such as brassinolide, naphthylacetic acid and chlormequat chloride, adding calcium nitrate, adding ammonium nitrate and evenly agitating to prepare powder compound plant ash; and adding water into the powder compound plant ash, keeping the humidity to be 12-20 percent, evenly mixing and pressing the compound plant ash into columnar materials to obtain the formed compound plant ash. The powder compound plant ash and the formed compound plant ash are used for dendrobium seedling culture. The compound plant ash is a high-efficacy fertilizer, the effect of slow release can be achieved at the same time, the fertility can be maintained more effectively, the compound plant ash is also a high-quality slow-release fertilizer, not only can ventilation be guaranteed, but also an excellent environment is provided for the growth of dendrobium seedlings through an upper layer and a lower layer, the growth of the dendrobium seedlings is promoted, the yield is improved, and the compound plant ash is particularly suitable for plants such as dendrobium which grow slowly and have harsh requirements on planting environments.

The invention relates to a brassinosteroid synthesizing rate-limiting gene for adjusting and controlling poplar wood formation and application of the brassinosteroid synthesizing rate-limiting gene, and belongs to the technical field of plant gene engineering. The nucleotide sequence of the brassinosteroid synthesizing rate-limiting gene is shown in a sequence 1 in a sequence table, and the aminoacid sequence of the brassinosteroid synthesizing rate-limiting gene is shown in a sequence 2 in the sequence table. The PagDET2 gene is transferred into a poplar tree, compared with wild-type polar tree, the transgenic polar tree with the PagDET2 of overexpression significantly grows tall and becomes thick, in addition, the cell number of the xylem of the transgenic polar tree is significantly increased, it is proved that the PagDET gene is a key adjusting gene for adjusting and controlling poplar wood formation, and the brassinosteroid synthesizing rate-limiting gene has important application value in the fields of forest-tree gene engineering and clonal forestry.

The invention discloses a method for reducing the content of H2O2 and MDA in non-heading Chinese cabbages after low-temperature stress, and relates to the technical field of genetic breeding. A mixedsolution (containing 0.1% Tween 80) of brassinolide (BRs) of 0.1 [mu]mol.L<-1> and strigolactone (SL) of 0.1 [mu]mol.L<-1> is sprayed. The contrast is clear water spraying and BRs and and SL spraying.The concentrations of BRs and SL are the same. According to all treatment, spraying is respectively carried out at 8: 00 and 20: 00 two days before the low temperature, and the spraying parts are theback surfaces of leaves, and spraying is carried out until the surfaces of the leaves drip water. Spraying is performed for four times in total. The mixed solution provided by the invention can effectively improve the low-temperature stress resistance of non-heading Chinese cabbage seedlings, and provides an effective guidance thought for improving the quality and yield of the non-heading Chinesecabbages after low-temperature stress.

The invention discloses a chemical regulation agent for corn growth. Each 1 kg of chemical regulation agent consists of the following materials: 18-22 mg of gibberellins, 10-20 g of potassium dihydrogen carbonate, 10-20 g of urea, 5-15 g of amino acid leaf fertilizer, 0.1-0.5 mg of brassinolide and the balance of water. According to the invention, the male and female interval of the tasseling stage and the silking stage of corn seedlings is shortened to improve the corn seedling pollination and the yield; besides, the chemical regulation agent facilitates the improvement on the grain filling rate, accelerates the fullness of grains, and is suitable for industrial production.

The invention discloses a special florescence prolonging medicament for a flower of a rape flower. The special florescence prolonging medicament for the flower of the rape flower is prepared from the following raw materials in parts by weight: 5 to 10 parts of 6-benzylaminopurine, 6 to 12 parts of naphthylacetic acid, 4 to 8 parts of 2, 4-dichlorphenoxyacetic acid, 2 to 4 parts of indoleacetic acid, 2 to 4 parts of gibberellin, 1 to 6 parts of aminoethoxyvinylglycine, 2 to 8 parts of aminooxyacetic acid, 1 to 3 parts of silver nitrate, 2 to 4 parts of silver thiosulfate, 5 to 12 parts of diethyl aminoethyl hexanoate, 5 to 8 parts of forchlorfenuron, 8 to 12 parts of compound sodium nitrophenolate, 7 to 10 parts of thiocarbamide, 6 to 10 parts of chloroethanol, 6 to 12 parts of hydrogen peroxide, 3 to 5 parts of brassinolide, 3 to 5 parts of triacontanol, 5 to 8 parts of paclobutrazol, and 1 to 2 parts of chlormequat chloride. The special florescence prolonging medicament for the flower of the rape flower has the beneficial effects that the special florescence prolonging medicament for the flower of the rape flower, which is provided by the invention, can effectively prolong the florescence of the flower, is further small in toxic action, and basically has no influence on an environment.

The invention discloses a method for increasing the embryogenesis rate of microspores of Wucai, and belongs to the field of vegetable chemical control. The method further comprises the step of spraying brassinolide to seedlings with 6-7 main leaves before obtaining the microspores of the Wucai, the concentration of the brassinolide added to a culture medium is 0.3 mg / L, the concentration of the brassinolide sprayed to the seedlings of the Wucai is 50 mg / L, and the spraying parts of the seedlings of the Wucai are the back surfaces of the leaves until the surfaces of the leaves drip water. The method provided by the invention can effectively improve the occurrence rate of microspore embryos of the Wucai.

The invention discloses a height-controlling composite growth regulating agent for corn and a preparation method thereof, and a method for applying the height-controlling composite growth regulating agent to corn, belonging to the field of agricultural production. The preparation method comprises the following steps: separately dissolving brassinolide, diethylaminoethyl hexanoate, ethephon and KH2PO4, then uniformly mixing the above dissolved substances under stirring so as to obtain a mixed solution, adding Tween 20 and carrying out dilution with water so as to obtain the height-controlling composite growth regulating agent for corn. The height-controlling composite growth regulating agent can obviously reduce the height of corn, improve the thickness of stalk, promote the development of aerial roots and lateral roots and increase the content of chlorophyll in corn leaves, achieves the purposes of height control, resistance to lodging and yield increase, and has the advantages of safety, environmental protection, no harm to crops, etc. Moreover, the preparation method is simple and suitable for large-scale production.

The invention discloses a special florescence prolonging medicament for common bletilla tuber flowers. The special florescence prolonging medicament is prepared from the following raw materials in parts by weight: 5-10 parts of 6-benzoyladenine, 6-12 parts of naphthaleneacetic acid, 4-8 parts of 2, 4-dichlorophenoxyacetic acid, 3-8 parts of ethephon, 2-6 parts of triiodobenzoic acid, 2-4 parts of indoleacetic acid, 2-4 parts of gibberellin, 1-6 parts of aminoethoxyvinylglycine, 2-8 parts of aminoxyacetic acid, 1-3 parts of silver nitrate, 2-4 parts of silver thiosulfate, 5-12 parts of diethyl aminoethyl hexanoate, 5-8 parts of forchlorfenuron, 8-12 parts of compound sodium nitrophenolate, 7-10 parts of thiourea, 3-5 parts of brassinolide, 3-5 parts of triacontanol, 5-8 parts of paclobutrazol and 1-2 parts of chlormequat chloride. The invention has the beneficial effects that the special florescence prolonging medicament for common bletilla tuber flowers, provided by the invention, can effectively prolong the florescence of the flowers, has small toxic and harmful effects and has almost no impact on the environment.

The invention discloses a purification method of plant endogenous brassinolide based on boron affinity solid phase extraction. According to the method, the plant endogenous brassinolide is extracted by a solvent, an extract liquid directly acts on a boron affinity material, the plant endogenous brassinolide is adsorbed on the surface of the material, matrix impurities are removed through leaching,and then the brassinolide adsorbed on the boron affinity material is eluted through an oxidation-hydrolysis-reverse phase elution strategy. The method is easy to operate, good in selectivity, high inrecovery rate, and thorough in removal of the matrix interference, can be used for high-sensitivity and quantitative analysis of trace brassinolide in plant materials, and has wide application prospects in plant genetics, crop science and other fields.

The invention discloses protein relevant to brassinolide signal conduction and a coding gene and the application thereof; the protein is the protein in the 1) or 2); 1) the protein is formed according to an amino acid sequence shown by a sequence 2 in a sequence table; 2) the amino acid residue sequence of the sequence 2 in a sequence table is substituted and / or lost and / or added by one or a plurality of the amino acid residues and is derived by 1) relevant to the plant tillering number. In the invention, DLT gene is cloned by utilizing a rice half-stuntedness little-tillering mutant, and molecule complementation verification is carried out; the result shows that DLT participates in the brassinolide signal conduction process to control the height, tillering and other phenotypes of the rice; after the DLT gene is transferred to the rice, the fact that the tillering number of the transgenosis rice with high DLT gene expression level is obviously increased; the invention has high potential application value on molecular breeding and improving crop production by utilizing genetic engineering measures to regulate plant hormone level.

The invention discloses a special florescence prolonging medicament for convallaria majalis flowers. The special florescence prolonging medicament is prepared from the following raw materials in parts by weight: 5-10 parts of 6-benzoyladenine, 6-12 parts of naphthaleneacetic acid, 4-8 parts of 2, 4-dichlorophenoxyacetic acid, 3-8 parts of ethephon, 2-4 parts of gibberellin, 1-6 parts of aminoethoxyvinylglycine, 2-8 parts of aminoxyacetic acid, 1-3 parts of silver nitrate, 2-4 parts of silver thiosulfate, 5-12 parts of diethyl aminoethyl hexanoate, 5-8 parts of forchlorfenuron, 8-12 parts of compound sodium nitrophenolate, 7-10 parts of thiourea, 6-10 parts of chloroethanol, 6-12 parts of hydrogen peroxide, 3-5 parts of brassinolide, 3-5 parts of triacontanol, 5-8 parts of paclobutrazol and 1-2 parts of chlormequat chloride. The invention has the beneficial effects that the special florescence prolonging medicament for convallaria majalis flowers, provided by the invention, can effectively prolong the florescence of the flowers, has small toxic and harmful effects and has almost no impact on the environment.

The invention provides a weeding method for millet planting. The weeding method comprises the following steps: selecting millet seeds and soaking the millet seeds with brassinolide with the concentration of 0.001-1mg / L; spraying a weed killer with the concentration of 7.5mg / L in farms after seeding, and spraying the brassinolide with the concentration of 0.0125-0.4mg / L on foliages after 24 hours, wherein the weed killer is a water solution of iodosulfuron-methyl-sodium.mesosulfuron-methyl water dispersible granules and alkyl ethyl sulfonate at a weight ratio of 9 to 1. According to the weeding method, the seeds are soaked with the brassinolide solution, so that the plant height and biomass of the grown millets can be improved, and the photosynthetic pigment content in seeding leaves of the millets can be reduced to a certain extent. According to the weeding method, the seeds and seedlings of the millets are processed with the brassinolide, so that the resistance of the millets to the weed killer is improved; modernized operation in the millet planting is convenient; the application range of the existing weed killer is expanded.

The invention discloses an iris tectorum maxim planting method which aims at solving the problem that seed propagation germination rate is low, a production period is long, root division propagation is limited by the seminal root number and the like in the conventional iris tectorum maxim planting process and accordingly the yield is not high and no available medicine can be adopted clinically. The iris tectorum maxim planting method adopts small iris tectorum maxim stem sections soaked by a brassinolide water solution to perform propagation through cuttage, the step of spraying the water solution outside roots is added, propagation scale is increased by 2-3 times compared with a conventional root division method, a planting period can be shortened, the yield can be improved by higher than 50% to the maximum extent, plant diseases and insect pests can be decreased, pesticide residue and environmental pollution are prevented, and the iris tectorum maxim planting method is simple in implementation, convenient and easy to operate, easy to master, low in input and large in harvesting amount.

The invention provides a method for relieving corn deep sowing stress by applying exogenous brassinosteroid, and relates to the field of corn safety production guarantee, and the method comprises material collection, BR treatment liquid preparation, seed treatment, deep sowing stress test, determination item and determination method, optimal concentration application and result analysis. According to the method, the relieving effect of the BR on the deep sowing stress of the corn seedlings under different concentrations is evaluated, the effect of relieving the deep sowing stress of the corn seedlings by the exogenous BR is scientifically, objectively and comprehensively evaluated by adopting a membership function method, a theoretical foundation is laid for improving the deep sowing resistance of the corn, a technical reference is provided for rapid application of the exogenous BR and screening of the optimal concentration, and the method has a wide application prospect. The application prospect is wide.

The invention discloses a plant growth regulator and application thereof. The plant growth regulator is prepared by mixing 6-benzylamino adenine, brassinolide, polyether modified trisiloxane and wateraccording to a ratio of (100-300)mg to (0.01-0.1)mg to (200-400)mg to 1L. After the two active components including 6-benzylamino adenine and brassinolide, the plant growth regulator is applied to leaves of orchidaceae plants, has obvious synergism, reaches ideal effect of increasing an amount of flower arrows of the orchidaceae plants, can increases the diameter of the flower arrows at the sametime, promotes flowering of butterfly orchids and remarkably improves the commodity of the orchidaceae plants. The plant growth regulator provided by the invention is particularly properly applied toindustrial cultivation of the orchidaceae plants including butterfly orchids. The plant growth regulator is liable in preparation and popularization as well as simple in use method.

The invention discloses a flowering extending agent special for iris japonica, which is prepared from the following raw materials by weight: 5-10 parts of 6-benzyl aminopurine, 6-12 parts of naphthalene acetic acid, 4-8 parts of 2,4-dichlorphenoxyacetic acid, 3-8 parts of ethephon, 2-6 parts of triiodobenzoic acid, 2-4 parts of indoleacetic acid, 2-4 parts of gibberellin, 1-6 parts of aminoethoxyvinylglycine, 2-8 parts of aminooxyacetic acid, 1-3 parts of silver nitrate, 2-4 parts of silver thiosulfate, 5-12 parts of diethylaminoethyl hexanoate, 5-8 parts of forchlorfenuron, 8-12 parts of sodium nitrophenolate, 6-12 parts of hydrogen peroxide, 3-5 parts of brassinolide, 3-5 parts of triacontanol, 5-8 parts of paclobutrazol, and 1-2 parts of chlormequat. The flowering extending agent can effectively extend the flowering time of flowers, is small in toxic action, and has no impact on the environment.

The invention relates to the field of micro algae energy, in particular to a method for promoting a micro algae frond to rapidly accumulate grease. The method comprises the following steps of 1, preparing an algae solution, wherein micro algae is cultured to the logarithm growth stage on the conditions that the temperature is 23-25 DEG C, the light intensity is 1,850-2,100 lx, the light and dark ratio is 12h / 12h, and the algae solution is obtained; 2, performing hormone induction, wherein brassinosteroid is added into 200-300 ml of algae solution in step 1 till the final concentration of the brassinosteroid is 20.2-21.5 mg / l; 3, obtaining the frond with the high grease content, wherein the algae solution obtained after hormone induction in step 2 is placed into a BG11 culture medium and cultured for 17-20 d on the condition that the temperature is 23-25 DEG C, the light intensity is 3,900-4,200 lx and light illumination is performed for 24 h continuously, and centrifuging and collecting are performed to obtain the frond with the high grease content; the method is easy to implement, free of contamination, high in efficiency, easy to realize and high in safety; in addition, the method can promote increase of grease content in micro algae, the grease conversion rate is greatly increased, and the method is beneficial to large-scale production and application.

The invention relates to a new application of an ester concentration complexing agent in improving saline-alkali stress resistance of apples. A product with brassinolide and strigolactone as effectivecomponents is applied to plants stressed by salt and alkali, so that the capability of the plants to resist the salt and alkali stress can be remarkably enhanced, the oxidative damage to the plants caused by the salt and alkali stress is reduced, and the yield and quality reduction caused by the salt and alkali are relieved.

The invention discloses quinoline amide compounds and a preparation method and application thereof. A structural formula of the quinoline amide compounds is shown as a formula I. According to biological activity verification, specific brassinolide reactions such as promotion of arabidopsis mutant det2-1 hypocotyl elongation in the dark and rice blade tilt angle increase, improvement of corn salt stress resistance and the like of some of the compounds are discovered; several compounds have high response values under the condition low concentration and are capable of inhibiting wheat plant height to retard growth and improve lodging resistance under the condition of high concentration; in addition, excellent effects in prevention and control of gramineous weeds such as wild oats, aegilops tauschii, barnyard grass, setaria viridis and aegilops. The quinoline amide compounds are easy to prepare, low in cost, high in agricultural application and promotion value and worthy of subsequent intensive research and development.

Compositions and methods for increasing plant yield, either directly or through selective inhibition of competing weeds, are disclosed herein. Particular focus is paid to cell cycle signaling permutations such as mutations in the DP-E2F-Rb cell cycle regulatory pathway, and to permutations in brassinolide pathways as transgenic or small-molecule targets for increasing plant yield.

The invention relates to a stable environment-friendly pesticide. The stable environment-friendly pesticide is prepared from the following raw materials in parts by weight: 6-14 parts of lavender oil, 3-9 parts of hairyvein agrimony, 4-8 parts of Cynanchum komarovii, 2-7 parts of rhizobium powder, 2-8 parts of lentinan, 4-9 parts of folium isatidis, 3-7 parts of dandelion, 2-6 parts of nitrobacteria powder, 1-6 parts of Cynanchum komarovii extract, 3-8 parts of common cnidium fruit, 5-7 parts of alkaloids of tripterygium wilfordii, 3-5 parts of synergist, 4-6 parts of brassinolide, 5-8 parts of purslane and 2-6 parts of pokeberry root. The stable environment-friendly pesticide prepared from the multiple plant ingredients has the beneficial effects of having non-toxic and pollution-free effects, being harmless to people and livestock and achieving an environment-friendly effect.

The invention relates to the technical field of wastewater treatment, in particular to a method for improving the toxicity resistance of microalgae in the wastewater treatment process through brassinolide. According to the invention, a trace amount of brassinolide is added, so that the toxicity resistance of microalgae is improved, the metabolic activity of microalgae is optimized, the yield of microalgae biomass in a wastewater biological treatment and microalgae biomass energy coupling technology is increased, and the synthesis of high-value additional products is directionally adjusted.

The invention provides a planting method of Chinese chives, and relates to the agricultural field. The planting method of the Chinese chives comprises the following steps: in the winter of the previous year when the Chinese chives are transplanted, soil is ploughed deeply by 30-45cm so as to remove weeds in the soil and expose worms in the soil; 6-9 days before seedlings of the Chinese chives are transplanted, the soil is ploughed deeply by 30-45cm again, and fertilizer is applied and mixed uniformly at the same time; 5500-5900 kilograms of organic fertilizer, 4-5 kilograms of urea and 6-9 kilograms of calcium superphosphate are applied in a field per mu; the seedlings of the Chinese chives are soaked in a brassinolide aqueous solution; the seedlings of the Chinese chives after being processed are transplanted into the soil, wherein 4-9 seedlings are planted in each hole, the row spacing is 20-35cm, and the distance between every two seedlings is 8-20cm; nitrogenous fertilizer is applied additionally after each time of harvesting. By means of the planting method of the Chinese chives, plant diseases and insect pests caused by reproduction of the Chinese chives for many years can be reduced; meanwhile, since the brassinolide aqueous solution is used for processing the seedlings of the Chinese chives, the output of the Chinese chives is increased compared with that in a conventional method, and the planting method is friendly to the environment.

The invention discloses a special flowering phase prolonging chemical for nerium oleander flowers. The special flower phasing prolonging chemical is prepared by adopting the following raw materials in parts by weight: 4 to 8 parts of 2,4-dichlorphenoxyacetic acid, 3 to 8 parts of ethephon, 2 to 6 parts of triiodobenzoic acid, 2 to 4 parts of indoleacetic acid, 2 to 4 parts of gibberellins, 1 to 6 parts of aminoethoxyvinylglycine, 2 to 8 parts of aminooxyacetic acid, 1 to 3 parts of silver nitrate, 2 to 4 parts of silver thiosulfate, 5 to 12 parts of diethylaminoethyl hexanoate, 5 to 8 parts of forchlorfenuron, 8 to 12 parts of compound sodium nitrophenolate, 7 to 10 parts of thiourea, 6 to 10 parts of ethylene chlorohydrins, 6 to 12 parts of hydrogen peroxide, 3 to 5 parts of brassinolide, 3 to 5 parts of triacontanol, 5 to 8 parts of paclobutrazol and 1 to 2 parts of chlormequat chloride. By adopting the special flowering phase prolonging chemical for the flowers, the flowering phase of the flowers can be effectively prolonged, the toxicity is small, and the environment is hardly affected.

The invention relates to the technical field of planting, and particularly discloses an alkali stress treatment agent and a preparation method and application thereof. The alkali stress treatment agent is prepared from brassinolide, melatonin and strigolactones, damage of alkali stress to plants can be effectively relieved through cooperative use of the brassinolide, melatonin and strigolactones, the problems that most of existing methods for relieving damage of alkali stress to plants are achieved by improving soil, the environment is easily polluted, and soil hardening is caused are solved. The provided preparation method is simple, the prepared alkali stress treatment agent can adapt to local conditions and efficiently solve the influence of soil alkalization on plant growth, the alkali stress resistance of plants can be improved when the alkali stress treatment agent is applied to leaf surfaces and / or roots of the plants, and the alkali stress treatment agent has wide market prospects.

The invention discloses a method for inducing a loblolly pine embryonic callus, and a dedicated culture medium thereof and belongs to the technical field of plant tissue culture. The method is characterized in that an immature loblolly pin seed is put on an inducing medium for inducing culture and an embryonic cell line with rapid growth and strong vigor is obtained, wherein the inducing culture medium takes a DCR culture medium as a basic culture medium and is added with 2,4-D, 6-BA, NAA, ABA, brassinolide, L-glutamine, casein hydrolysate, inositol, and 2-(N-morpholine) ethanesulfonic acid monohydrate. The invention provides the stable and efficient method for inducing the loblolly pin embryonic callus, wherein the yield and quality of the embryonic callus are increased, subculture multiplication of the embryonic callus is normal, good embryonic natures are kept, and important technological support is provided for further implementation of studies in somatic embryo seedling culture, genetic transformation, germplasm innovation and the like.

The invention discloses a bactericide for controlling rice sheath blight disease. The bactericide is prepared from raw materials in parts by weight as follows: 5-8 parts of picoxystrobin, 25-35 parts of traditional Chinese medicine extract, 0.8-1.2 parts of methyl jasmonate and 0.4-0.8 parts of brassinosteroid, 2-4 parts of a penetrant, 16-18 parts of a dispersant, 8-10 parts of glycerin and 100-200 parts of water, wherein the traditional Chinese medicine extract comprises raw materials as follows: radix phytolaccae, herba asari, fructus viticis, semen ginkgo, fructus forsythia oil, cortex cinnamomi oil and rhizoma zingiberis oil. The bactericide does not produce drug tolerance and has good effect on controlling bacterial blight of rice.

A method of treating a solid tumor in a mammal by inhibiting angiogenesis, including administering to the mammal which has a solid tumor selected from the group consisting of breast carcinoma, lung carcinoma, prostate carcinoma, colon carcinoma, ovarian carcinoma, neuroblastoma, central nervous system tumor, multiform glioblastoma and melanoma; a composition including brassinosteroids of general formula (I)wherein can be a single or double bond and the configurations of carbon atoms C22 and C23 respectively linked to the substituents HO are S for both carbon atoms and a pharmaceutically acceptable additive.