60 results about "Brassinine" patented technology

The invention provides a high-salinity high-tidal-flat mangrove forest ecological restoration method. The high-salinity high-tidal-flat mangrove forest ecological restoration method comprises the following steps of S1, soil improvement, S2, seedling cultivation, S3, seedling planting, S4, seedling reseeding, S5, bank protection device arrangement and S6, pest control. The soil conditioner is used for improving the soil structure and reducing the soil salinity. Salt-resistant domestication is performed on the seedlings by gradually improving the salinity of irrigation water, so that the salt resistance of the seedlings is improved. Organic fertilizer, ferriporphyrin, abscisic acid and brassinolide are used for providing nutrients for seedlings, osmotic equilibrium in plants is adjusted, opening and closing of air holes are adjusted, plasticity of cell walls is adjusted, and therefore the salt resistance of the plants is enhanced. According to the method, the roots of seedlings are inoculated with the halorhodobryum terbach and the proteobacteria, the halorhodobryum terbach and the proteobacteria terbach form a symbiotic relationship with the roots of plants, a microbial community is formed, and the absorption efficiency of the plants to nutrients in soil is According to the method, the planting survival rate and the plant growth rate are improved through the multi-way overall synergistic effect, and then the biological diversity is improved.

The invention belongs to the field of fertilizers, and particularly relates to a foliar fertilizer with high amino acid synthesis efficiency and a preparation method thereof.The preparation method comprises the steps that spermidine is taken to be subjected to formic acid esterification modification, and formic acid esterification spermidine is obtained; the preparation method comprises the following steps: hydrolyzing 2, 4-epibrassinolide to obtain carboxylated brassinolide; under the action of Tween, carboxylated brassinolide and formic acid esterified spermidine are combined to form a fertilizer base full of emulsified liquid drops; and adding other nutrients to obtain the foliar fertilizer. The leaf fertilizer for increasing the amino acid content of rice can increase the content of amino acids, especially lysine, in grains on the premise of increasing the yield of rice, and nitrogen absorption and utilization of the rice grains are improved.

The invention discloses a tea tree CsBZR1 gene and cold resistance application thereof, the tea tree CsBZR1 gene is a core gene in a tea tree brassinolide signal transduction pathway, and the nucleotide sequence of the tea tree CsBZR1 gene is shown as SEQ ID NO.1 in a sequence table. According to the application of the CsBZR1 gene of the tea tree to regulation and control of cold resistance of the tea tree, the low-temperature tolerance of the tea tree can be reduced by inhibiting expression of the CsBZR1 gene in vivo, and the cold resistance of arabidopsis thaliana is improved by overexpression of the CsBZR1 gene. Expression of CsBZR1 of a tea tree under a low-temperature condition is directly induced to be up-regulated by low-temperature stress of the tea tree, cold resistance of arabidopsis thaliana is improved by overexpression of the gene in the arabidopsis thaliana, and low-temperature damage of the tea tree can be remarkably aggravated by inhibiting expression of CsBZR1 in the tea tree through an antisense oligonucleotide technology. The cloning of the gene not only is beneficial to determining the effects of brassinolide and the signal transduction core factor BZR1 thereof in the cold-resistant process of the tea tree, but also is beneficial to promoting the genetic improvement process aiming at enhancing the cold resistance of the tea tree.

The invention relates to the technical field of biology, in particular to application of brassinolide in increasing the yield of lettuce latex. According to the invention, brassinolide is adopted as an active component and acts on lettuce plants, so that the yield of lettuce latex can be remarkably increased, and meanwhile, the contents of sucrose and isopentenyl pyrophosphate in lettuce are further remarkably increased. Compared with indoleacetic acid, the brassinolide provided by the invention is more suitable for improving the gum production performance of the plant lettuce, and the brassinolide and the indoleacetic acid are high in adaptability, so that a more excellent technical effect is obtained. In the use process, the use method of brassinolide is simple, the use amount is small, the function of remarkably improving the yield of lettuce latex, sucrose and isopentenyl pyrophosphate can be achieved by adopting brassinolide with low concentration, the cost is low, the effect is good, no new toxic or side effect is caused to the environment, and the brassinolide is suitable for application and popularization.

The invention discloses a management method for improving cold resistance and drought resistance of macadamia nuts and increasing flowering quantity, which combines a four-stage dynamic management mode of soil improvement, cold resistance induction, drought resistance and tip control and flowering phase quality improvement, applies an organic fertilizer, a water-retaining agent and a composite slow-release fertilizer, induces root development, slowly releases the fertility of a chemical fertilizer, prolongs the action time of the chemical fertilizer, and improves the yield of the macadamia nuts. Brassinolide and potassium fulvate are compounded to activate cold-resistant gene expression of macadamia nut plants and improve cell membrane stability, so that cold resistance of the macadamia nut plants is improved, melatonin and methyl jasmonate perform double shoot control, an ABA signal channel of the plants is triggered, drought resistance of the plants is improved, flower bud differentiation is promoted, and growth of the macadamia nut plants is promoted. The synergistic effect of alginate oligosaccharide and gamma-aminobutyric acid enhances the elongation ability of a pollen tube, chemical agent stimulation is replaced by biological agent stimulation, the utilization rate of chemical agents is reduced, then the negative influence of the chemical agents on soil is reduced, and finally the fruit setting rate and the sustainable utilization of the soil are improved.

The invention provides an application of an XTH gene in promotion of grape fruit hardness formation by brassinolide, brassinolide (BR) is adopted for treatment in a grape color changing period, it is found that the XTH gene can mediate synthesis of hemicellulose in grape fruits so as to significantly improve the hardness of the grape fruits, and through whole genome correlation analysis and transcriptomics analysis, the XTH gene can be used for promoting the hardness formation of the grape fruits. The two target genes VvXTH25 and VvXTH31 capable of regulating and controlling the hardness of the grape fruits are found, key genes and mechanisms for regulating and controlling the hardness of the grape fruits are defined, the hardness of the fruits is regulated and controlled by regulating and controlling the content of hemicelluloses, and the VvXTH25 and VvXTH31 have the effects of providing a basis and a means for regulating and controlling the texture of the grape fruits and improving the storage and transportation resistance; a brand-new way is provided for really regulating and controlling the hardness of the grape fruits.

A plant medium composition and methodology of application of the medium as it pertains to the micropropagation of Cannabis and related plant species is described that yields two to four-fold increase in the number of biologically functional nodes from axillary shoots thereby allowing for exponential amplification of such plant species. The compositions comprise basal plant media supplemented with: (i) one or more cytokinins, and (ii) one or more gibberellins and / or brassinolides. This achievement in exponential shoot growth of these plant species represents a marked step forward in the Cannabis industry as it pertains to commercial micropropagation of a recreationally and medicinally important plant whose extracts are the subject of numerous clinical trials.

The invention discloses application of brassinolide in improving resistance of sugarcane to smut. According to the method, brassinolide is sprayed on leaf surfaces, so that the brassinolide can improve the resistance of sugarcane to smut caused by sugarcane smut, the morbidity of sugarcane plants infected by sugarcane smut is remarkably reduced, and the influence of sugarcane smut on the yield and quality of sugarcane is reduced. Therefore, the invention provides application of brassinolide in improving or preparing a product for improving the resistance of sugarcane to smut. The method is beneficial to green control of sugarcane smut, and is simple to operate and good in feasibility.

The invention provides an anti-cracking agent for preventing seedless litchi fruits from cracking before picking, and particularly relates to the technical field of crop cultivation. The anti-cracking agent for preventing the seedless litchi fruits from cracking before picking comprises a component A, a component B and a component C, the component A is one of thidiazuron or forchlorfenuron, the component B adopts a brassinolide compound, the component C is a nitrate nitrogen fertilizer, and the brassinolide compound is 24-epibrassinolide. The anti-cracking composition has the beneficial effects that thidiazuron and forchlorfenuron in the anti-cracking composition are efficient plant cytokinin and can efficiently promote plant tissue cell division, brassinosteroid compounds with low concentration can remarkably promote crop growth, calcium ammonium nitrate promotes pericarp growth and increases pericarp toughness, the thidiazuron, forchlorfenuron and brassinosteroid compounds are used cooperatively, and the anti-cracking effect is good. The seed-free lychee anti-cracking agent can achieve an obvious synergistic effect on prevention of seed-free lychee cracking, can rapidly promote fruit peel cell division of fruits, enables fruit peels to grow faster, improves the toughness of the fruit peels, and is good in anti-cracking effect before picking.

The invention discloses a tissue culture detoxification method of Amomum tsao-ko, which comprises the following steps: S1, selecting an Amomum tsao-ko plant which grows robustly, taking a tender stem segment of the Amomum tsao-ko plant as an explant, disinfecting, inoculating into a callus induction culture medium, culturing, and inducing the generation of callus; wherein the callus induction culture medium is prepared from 0.8 mg / L to 1.2 mg / L of MS + 2, 4-D, 0.4 mg / L to 0.6 mg / L of 6-BA and 3 mu mol / L to 5 mu mol / L of chicoric acid; s2, heat treatment: cutting the callus obtained in the step S1 into small pieces, and putting the small pieces into sterile water at 40 + / -2 DEG C for heat bath for 15-20 minutes; s3, differentiation culture: inoculating the callus subjected to the hot bath treatment into a differentiation culture medium, and inducing tissue differentiation; wherein the differential culture medium is prepared from 1.2 mg / L to 1.8 mg / L of 1 / 2 MS, 6-BA and 0.04 mg / L to 0.06 mg / L of TDZ; s4, inducing rooting: cutting cluster buds obtained through differentiation culture into single buds without callus tissues, and inoculating the single buds into a rooting culture medium; wherein the rooting culture medium is prepared from 1 / 2 MS, 1.0 mg / L to 1.4 mg / L of IBA, 0.2 mg / L to 0.4 mg / L of NAA and 0.04 mg / L to 0.07 mg / L of brassinolide. By using the method provided by the invention, the tomato spotted wilf virus detoxification success rate in the tsaoko amomum fruit tissue culture process can be effectively improved.

The invention discloses a cutting seedling rooting agent and a preparation method thereof, and relates to the technical field of garden horticulture, and the cutting seedling rooting agent comprises the following raw materials by weight: 5-15% of a citrus pectin oligosaccharide-spermidine compound, 0.0001-0.0005% of a brassinolide nanoemulsion, 2-5% of proline, 3-8% of sugar alcohol calcium, 5-10% of modified attapulgite, 0.25-0.55% of a surfactant, and the balance of deionized water. After use, the rooting effect is good and the stability is high.

The invention discloses a macadamia nut fruit retention and fruit setting fertilizer based on hormone synergistic regulation and an application method thereof, and relates to the technical field of fertilizers. The fertilizer comprises a first component, a second component, a third component and a fourth component, the first component is prepared from the following raw materials: urea, vermiculite, water-retaining composite gel, humic acid, pseudomonas fluorescens, actinomycetes and decomposed straw; the second component raw materials comprise stimulant, gibberellin, auxin, sodium thiosulfate, mineral oil, polyvinyl alcohol, calcium chloride, potassium silicate, boric acid and brassinosteroid; a third component raw material: a medium-nitrogen low-phosphorus high-potassium compound fertilizer and a medium-trace element fertilizer; and the fourth component comprises the following raw materials: compound sodium nitrophenolate, isopentenyl adenine, glycine betaine, mineral oil and polyvinyl alcohol. The fertilizer disclosed by the invention can provide sufficient water and nutrients in a young fruit stage, reduces the physiological fruit dropping rate in the early stage and the middle and later stages, can promote protein and fat accumulation in a fruit expansion stage and a fruit maturation stage, and reduces the physiological fruit dropping rate in the middle and later stages.

This invention relates to the field of biotechnology, specifically disclosing a method for improving the biocompatibility of poplar trees. PagBEH3b Methods for promoting poplar lignite development and / or increasing poplar height and / or increasing poplar yield through gene expression. This invention involves... PagBEH3b Genes were transferred into poplar trees and overexpressed. PagBEH3b Compared with wild-type, transgenic poplar showed a significant increase in xylem, indicating that the xylem of the brassinolide signaling pathway is a key regulator of this pathway. PagBEH3b It is a key regulatory factor in controlling xylem development in poplar. This invention provides a new option for regulating xylem development and has significant application value in the field of forest tree genetic engineering.

The invention discloses an SlFMO1 gene and application thereof in regulation and control of growth and development of tomato plants, and belongs to the technical field of gene engineering. According to the method, a CRISPR / Cas9 gene editing technology is utilized, firstly, a CRISPR / Cas9-gRNA vector is constructed to accurately edit an SlFMO1 gene in tomatoes, GV3101 agrobacterium tumefaciens is utilized to conduct mediated transformation on the tomatoes, and kanamycin serves as a resistance marker to conduct screening to obtain SlFMO1-deleted mutant tomatoes. Phenotype observation and analysis show that after the SlFMO1 gene is knocked out, the plant height and the plant width of a tomato plant are obviously increased, the apical development is enhanced, the stem diameter of the plant is increased, and the inflorescence spacing is increased, which indicates that the SlFMO1 gene negatively regulates the growth and development of the tomato, and meanwhile, the expression of related genes synthesized by auxin, gibberellin and brassinolide is obviously enhanced. The discovery has important theoretical significance and practical application value for follow-up cultivation of ideal tomato varieties.

The invention relates to a method for promoting centella asiatica seed germination, and belongs to the technical field of medicinal plant cultivation. Aiming at the technical problems of low germination rate and long period of centella asiatica seeds caused by seed coat obstacles and endogenous inhibitors, the method comprises the following steps: firstly, sequentially carrying out mechanical coat breaking, ultrasonic treatment and high-pressure permeation treatment on the seeds to break a physical barrier; mixing the seeds with a wet substrate, carrying out warm lamination at 18-25 DEG C for 25-35 days, then carrying out cold lamination at 2-6 DEG C for 50-70 days, and spraying a KNO3 solution in the cold lamination stage to activate physiological metabolism; after lamination, washing with running water, sequentially soaking with a thiourea solution, carrying out H2O2 treatment for chemical degradation, and carrying out biological adsorption by adopting an adsorbent containing bacillus subtilis and activated carbon; soaking the seeds into a mixed solution containing brassinolide, kinetin and fluridone, and carrying out gradient heating and drying; and finally carrying out germination culture. The method can significantly improve the germination efficiency and uniformity, and is suitable for centella asiatica large-scale seedling raising and medicinal resource development.

The invention provides a plant biostimulant composition for increasing the carotenoid content of fruits and vegetables as well as a preparation method and a use method of the plant biostimulant composition. The plant biostimulant composition is prepared from the following raw materials in parts by weight: 0.01 to 0.1 part of brassinolide (BR), 50 to 200 parts of sodium sulfite (Na2SO3), 1000 to 2000 parts of ammonium polyphosphate (APP), 50 to 200 parts of APP-manganese chelate (in terms of Mn element), 500 to 2000 parts of APP-magnesium chelate (in terms of Mg element) and 25 to 100 parts of organic silicon additive. The plant biostimulant composition is sprayed on the leaf surfaces of fruit trees, so that crop bodies can be stimulated to increase synthesis and storage of carotenoids, the content of the carotenoids in crops is increased, accumulation of flavor substances is increased, and the quality of fruits and vegetables is improved.

The present invention provides a method for improving the survival rate of rice radiation-induced mutagenesis seedlings and / or promoting the growth of rice radiation-induced mutagenesis seedlings, and belongs to the field of agricultural technology. The present invention provides the use of brassinolide in improving the survival rate of rice radiation-induced mutagenesis seedlings and / or promoting the growth of rice radiation-induced mutagenesis seedlings. Radiation treatment of rice seeds to create mutants is one of the important ways to innovate germplasm resources. However, irradiating rice seeds will affect their physiological metabolic activities, resulting in abnormal growth or even death of seedlings, thereby significantly reducing the mutagenesis efficiency. The present invention utilizes the plant growth regulator brassinolide to treat rice seeds at different growth stages after radiation treatment, which can improve the survival rate of induced seedlings, reduce the loss of mutant genes, and thus improve the efficiency of radiation mutagenesis.

The invention discloses a plant underground propagule excitant, and belongs to the technical field of excitant preparation. The plant underground propagule excitant is composed of 169.91-196.13 parts of a mixture A and 2-4 parts of an immobilized microbial agent, and the mixture A is composed of the following components in parts by weight: 0.1-0.2 part of brassinolide, 1-2 parts of sodium nitroprusside, 30-40 parts of cane sugar, 70-80 parts of urea, 68-73 parts of disodium hydrogen phosphate and 0.81-0.93 part of a composite bioactive matter. According to the plant underground propagule excitant prepared by the method, the grassland improvement effect exceeds the expectation, the aboveground biomass is increased by 1-2.5 times, the root biomass is increased by 2-3 times, and the density of vegetation plants (clusters) is increased by 2-3 times.

The invention discloses a synergist capable of improving the overwintering rate of alfalfa and a planting method, and relates to the technical field of agriculture, the synergist comprises the following raw materials: brassinolide, chitosan oligosaccharide coated riboflavin microcapsule powder, brown sugar and water in a mass ratio of (0.5-1.5): (1-3): (6-14): (800-1200); the planting method comprises the following steps: step 1, preparing an alfalfa field for crop rotation, cutting the overground part, integrally taking out root necks and branch buds, cutting the root necks and the branch buds of a whole plant into alfalfa bud particles with the diameter of 0.5-1cm through mechanical equipment, and spraying the alfalfa bud particles with a synergist; 2, the alfalfa bud particles subjected to spraying treatment are sown, the sowing depth ranges from 2 cm to 3 cm, the row spacing of a forage grass planting field ranges from 15 cm to 20 cm, the row spacing of a seed planting field ranges from 30 cm to 40 cm, and the sowing rate ranges from 100 kg / mu to 200 kg / mu. According to the synergist and the planting method provided by the invention, the cold resistance and stress resistance of alfalfa can be effectively improved, the overwintering rate is increased, and the utilization of an alfalfa bud bank is realized.

This invention relates to compositions and methods for modifying Brassinosteroid Insensitive-1 (BRI1) genes in plants by introducing at least one mutation in a region of the BRI1 gene encoding the kinase domain, optionally to improve yield traits. The invention further relates to a mutated BRI1 gene, a modified BRI1 polypeptide, and plants having increased improved yield traits produced using the methods and compositions of the invention.

The invention discloses a production method for regulating and controlling Yunnan purple garlic test tube bulbs by brassinolide. According to the method, the Yunnan purple garlic rachis is taken as an explant, and efficient production is realized by establishing a technical system of cluster seedling induction, differentiation and growth and test tube bulb formation. The following culture medium conditions are specifically adopted: a cluster seedling induction culture medium is MS + 1 mg.L <-1 > KT + 0.1 mg.L <-1 > NAA + 0.1 mg.L <-1 > BR + 30.0 g.L <-1 > sucrose + 7.5 g.L <-1 > agar, and the pH is 6.2; the test tube bulb induction culture medium is MS + 90 g.L <-1 > sucrose + 6.8 g.L <-1 > agar, and the pH value is 7.5. Under the system, the adventitious bud induction rate of the clustered seedlings and the formation rate of the test-tube bulbs are up to 98.14% or above, the obtained test-tube bulbs meet detoxification standards, each rachis can produce 16.89 bulbs at most, and the bulbs have growing points and germination potential. In addition, the soluble sugar content of the bulbs is reduced by 50.31%, and long-term storage is facilitated. The method provides reliable technical support for detoxification and rapid propagation breeding of the Yunnan purple garlic.

The invention belongs to the technical field of plant genetic engineering and the technical field of soybean breeding, and particularly discloses application of a soybean brassinolide inactivating enzyme encoding gene GmBAS1C in regulation and control of the soybean branch number, the CDS sequence of the soybean brassinolide inactivating enzyme encoding gene GmBAS1C is shown as SEQ ID No.1, the amino acid sequence of the encoded protein is shown as SEQ ID No.2, and the amino acid sequence of the soybean brassinolide inactivating enzyme encoding gene GmBAS1C is shown as SEQ ID No.3. The invention further discloses application of the soybean brassinolide inactivating enzyme encoding gene GmBAS1C in regulation and control of the soybean branch number. The gene is located in an endoplasmic reticulum, and formation and development of lateral branches are promoted by up-regulating expression quantities of genes such as brassinolide synthesis genes CPD, ROT3 and BR6OX2, auxin response genes IAA, GH3 and SAUR and the like. The invention also discloses three homozygous gene editing mutants (KOGmBAS1C-1, KOGmBAS1C-2 and KOGmBAS1C-3) of the GmBAS1C gene, a soybean plant containing the gene editing mutants, and a vector construction method of the soybean plant. The invention further discloses the three homozygous gene editing mutants (KOGmBAS1C-1, KOGmBAS1C-2 and KOGmBAS1C-3) of the GmBAS1C gene. Experiments prove that the number of branches of homozygous gene editing mutant phenotypes of the three GmBAS1C genes is remarkably increased. The invention provides a new gene target, a molecular tool and a breeding material for soybean high-branch breeding, and has important agricultural application value.

The invention discloses a material-saving-free cultivation method and application of eucalyptus tomentosa seedlings. The cultivation method comprises the step of spraying an exogenous hormone aqueous solution in stages in the 3-8-month-old period of the eucalyptus tomentosa seedlings: (1) at the first stage, spraying a gibberellin aqueous solution; (2) a second stage: spraying a brassinolide aqueous solution; and (3) a third stage: spraying a mixed aqueous solution containing brassinolide and abscisic acid. The material-saving-free cultivation method for the eucalyptus tomentosa nursery stock is used for material-saving-free eucalyptus tomentosa cultivation, synchronous regulation and control of trunk height promotion and lateral branch inhibition of the eucalyptus tomentosa nursery stock can be achieved, the trunk cleaning height and the trunk cleaning rate are remarkably improved, and early-stage material-saving-free formation of the eucalyptus tomentosa is promoted.

The invention belongs to the technical field of botanical herbicides, and provides spirobrassinolide as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing isatin, nitromethane, a chiral catalyst and tetrahydrofuran, and performing asymmetric Henry reaction to obtain an intermediate 1; mixing the intermediate 1, methanol and a palladium-carbon catalyst, and performing catalytic hydrogenation reduction reaction to obtain an intermediate 2; mixing the intermediate 2, carbon disulfide, a sodium hydroxide solution and methyl iodide, and carrying out an addition methylation reaction to obtain an intermediate 3; and mixing the intermediate 3, dichloromethane, pyridine and methylsulfonyl chloride, and carrying out a substitution cyclization reaction to obtain the spirobrassinolide. The process route is efficient and excellent in stereoselectivity, the problem of low efficiency of traditional natural product separation is avoided, and the prepared spirobrassinolide is high in weed inhibition activity, has a remarkable inhibition effect on growth of gramineous weed millet and broadleaf weed chenopodium album and has the potential of becoming a novel plant source herbicide.

The invention provides brassinosterols and application of an inhibitor of the brassinosterols in regulating and controlling the de novo regeneration of dicotyledonous plant root organs, and belongs to the technical field of plant growth regulation and control. According to the invention, brassinosteroid (BR) related mutants are used as materials, and the function of BR in the de novo regeneration process of roots is explored through an in-vitro leaf root regeneration induction method. The result shows that the BR signal can inhibit the root regeneration process, based on this, the invention provides the application of the brassinosteroid synthesis inhibitor in promoting generation of root organs of dicotyledonous plant in-vitro tissue and the application in dicotyledonous plant in-vitro culture, the brassinosteroid synthesis inhibitor is exogenously added, and the yield of the brassinosteroid synthesis inhibitor is improved. The rooting process of the dicotyledonous plant in-vitro leaves can be promoted, the in-vitro culture process is accelerated, and the application value is high.

The application discloses a large-scale cutting of ginkgo and a cutting method thereof, and the cutting method comprises the following steps: cutting and dwarfing pruning a 4-5 year old ginkgo mother plant, pruning the lateral branches of the cut mother plant for multiple times, spraying a plant growth regulator after each pruning to promote the development of the lateral branches of the mother plant, cutting the branches on the lateral branches of the mother plant as cuttings, and inserting the cuttings into a substrate after treating the cuttings with a plant growth regulator. The method adopts the cutting and multiple pruning to promote the sprouting of the plant and generate the juvenile branch, greatly increases the number of cuttings, sprays 6-benzyladenine or brassinolide to improve the quality and quantity of the cuttings, and further uses methyl jasmonate, naphthalene acetic acid and uniconazole to jointly treat the cuttings to significantly improve the rooting rate of the cuttings. The method can be used for cultivating the leaf ginkgo and the ginkgo mother plant with excellent characteristics, significantly shortens the formation time of the cutting callus and adventitious roots, is simple and easy to operate, is suitable for large-scale cutting, and has popularization value in the asexual propagation of the ginkgo clone.

The present invention relates to the field of genetic engineering technology, and in particular to the use of the GmDET2-1 protein and its encoding gene in increasing the oil content of soybean seeds. The present invention is the first to discover that the soybean brassinosteroid synthase GmDET2-1 protein or its encoding gene can regulate the oil content of plant seeds. This has high practical application value in plant variety improvement or breeding.

The application provides application of an XTH gene in promotion of grape fruit hardness formation by brassinosteroids, finds that brassinosteroids (BR) can mediate synthesis of hemicellulose in grape fruits and thus significantly improve the hardness of the grape fruits by treating the grape fruits with the brassinosteroids at a veraison stage, finds two target genes VvXTH25 and VvXTH31 capable of regulating the hardness of the grape fruits through whole genome correlation analysis and transcriptome analysis, and determines the key genes and mechanism for regulating the hardness of the grape fruits, which regulates the hardness of the grape fruits by regulating the content of hemicellulose, has the action of providing the basis and means for regulating the texture of the grape fruits and improving the storage and transportation capacity, and provides a brand-new way for truly regulating the hardness of the grape fruits.

The invention discloses an application of 24-Epi brassinolide in promoting the development of female germline stem cells. Belongs to the technical field of reproductive biology. Female germline stem cells (FGSCs) are important cell models for studying the development of germ cells, however, the regulation and control mechanism of natural products on the development of the FGSCs still needs to be deeply explored. The invention aims at disclosing the regulation and control effect of 24-Epi brassinolide (24-Epibrassinolide, EBR) on FGSCs development and a molecular mechanism of the 24-Epi brassinolide on the FGSCs development by utilizing an FGSCs in-vitro development system and combining LongRNA-seq and RIC-seq technologies, and a new strategy is provided for treatment of infertility and optimization of a clinical treatment scheme.

A biochar-seed gel for vegetation rehabilitation in saline-alkali soil, a preparation method thereof, and an application thereof are provided. The biochar-seed gel includes a water-retaining hydrogel, effective nutrient components, and a structural maintenance agent, where the water-retaining hydrogel is polyacrylamide; the effective nutrient components include Bacillus subtilis inoculant, brassinolide, chitosan oligosaccharide, polyaspartic acid, and compound fertilizer; and the structural maintenance agent includes biochar, clay, chitosan, and Gleditsia sinensis powder. The biochar-seed gel forms a microenvironment on the soil surface, mitigating stress caused by high-salinity soil conditions. The gel exhibits strong water-retention capacity, effectively maintaining moisture to support seed and seedling growth while alleviating water deficiency resulting from soil compaction.