52 results about "Brassinine" patented technology

The invention belongs to the field of fertilizers, and particularly relates to a foliar fertilizer with high amino acid synthesis efficiency and a preparation method thereof.The preparation method comprises the steps that spermidine is taken to be subjected to formic acid esterification modification, and formic acid esterification spermidine is obtained; the preparation method comprises the following steps: hydrolyzing 2, 4-epibrassinolide to obtain carboxylated brassinolide; under the action of Tween, carboxylated brassinolide and formic acid esterified spermidine are combined to form a fertilizer base full of emulsified liquid drops; and adding other nutrients to obtain the foliar fertilizer. The leaf fertilizer for increasing the amino acid content of rice can increase the content of amino acids, especially lysine, in grains on the premise of increasing the yield of rice, and nitrogen absorption and utilization of the rice grains are improved.

The invention relates to the technical field of biology, in particular to application of brassinolide in increasing the yield of lettuce latex. According to the invention, brassinolide is adopted as an active component and acts on lettuce plants, so that the yield of lettuce latex can be remarkably increased, and meanwhile, the contents of sucrose and isopentenyl pyrophosphate in lettuce are further remarkably increased. Compared with indoleacetic acid, the brassinolide provided by the invention is more suitable for improving the gum production performance of the plant lettuce, and the brassinolide and the indoleacetic acid are high in adaptability, so that a more excellent technical effect is obtained. In the use process, the use method of brassinolide is simple, the use amount is small, the function of remarkably improving the yield of lettuce latex, sucrose and isopentenyl pyrophosphate can be achieved by adopting brassinolide with low concentration, the cost is low, the effect is good, no new toxic or side effect is caused to the environment, and the brassinolide is suitable for application and popularization.

The invention discloses a management method for improving cold resistance and drought resistance of macadamia nuts and increasing flowering quantity, which combines a four-stage dynamic management mode of soil improvement, cold resistance induction, drought resistance and tip control and flowering phase quality improvement, applies an organic fertilizer, a water-retaining agent and a composite slow-release fertilizer, induces root development, slowly releases the fertility of a chemical fertilizer, prolongs the action time of the chemical fertilizer, and improves the yield of the macadamia nuts. Brassinolide and potassium fulvate are compounded to activate cold-resistant gene expression of macadamia nut plants and improve cell membrane stability, so that cold resistance of the macadamia nut plants is improved, melatonin and methyl jasmonate perform double shoot control, an ABA signal channel of the plants is triggered, drought resistance of the plants is improved, flower bud differentiation is promoted, and growth of the macadamia nut plants is promoted. The synergistic effect of alginate oligosaccharide and gamma-aminobutyric acid enhances the elongation ability of a pollen tube, chemical agent stimulation is replaced by biological agent stimulation, the utilization rate of chemical agents is reduced, then the negative influence of the chemical agents on soil is reduced, and finally the fruit setting rate and the sustainable utilization of the soil are improved.

The invention provides an application of an XTH gene in promotion of grape fruit hardness formation by brassinolide, brassinolide (BR) is adopted for treatment in a grape color changing period, it is found that the XTH gene can mediate synthesis of hemicellulose in grape fruits so as to significantly improve the hardness of the grape fruits, and through whole genome correlation analysis and transcriptomics analysis, the XTH gene can be used for promoting the hardness formation of the grape fruits. The two target genes VvXTH25 and VvXTH31 capable of regulating and controlling the hardness of the grape fruits are found, key genes and mechanisms for regulating and controlling the hardness of the grape fruits are defined, the hardness of the fruits is regulated and controlled by regulating and controlling the content of hemicelluloses, and the VvXTH25 and VvXTH31 have the effects of providing a basis and a means for regulating and controlling the texture of the grape fruits and improving the storage and transportation resistance; a brand-new way is provided for really regulating and controlling the hardness of the grape fruits.

The invention discloses application of brassinolide in improving resistance of sugarcane to smut. According to the method, brassinolide is sprayed on leaf surfaces, so that the brassinolide can improve the resistance of sugarcane to smut caused by sugarcane smut, the morbidity of sugarcane plants infected by sugarcane smut is remarkably reduced, and the influence of sugarcane smut on the yield and quality of sugarcane is reduced. Therefore, the invention provides application of brassinolide in improving or preparing a product for improving the resistance of sugarcane to smut. The method is beneficial to green control of sugarcane smut, and is simple to operate and good in feasibility.

The invention discloses a tissue culture detoxification method of Amomum tsao-ko, which comprises the following steps: S1, selecting an Amomum tsao-ko plant which grows robustly, taking a tender stem segment of the Amomum tsao-ko plant as an explant, disinfecting, inoculating into a callus induction culture medium, culturing, and inducing the generation of callus; wherein the callus induction culture medium is prepared from 0.8 mg / L to 1.2 mg / L of MS + 2, 4-D, 0.4 mg / L to 0.6 mg / L of 6-BA and 3 mu mol / L to 5 mu mol / L of chicoric acid; s2, heat treatment: cutting the callus obtained in the step S1 into small pieces, and putting the small pieces into sterile water at 40 + / -2 DEG C for heat bath for 15-20 minutes; s3, differentiation culture: inoculating the callus subjected to the hot bath treatment into a differentiation culture medium, and inducing tissue differentiation; wherein the differential culture medium is prepared from 1.2 mg / L to 1.8 mg / L of 1 / 2 MS, 6-BA and 0.04 mg / L to 0.06 mg / L of TDZ; s4, inducing rooting: cutting cluster buds obtained through differentiation culture into single buds without callus tissues, and inoculating the single buds into a rooting culture medium; wherein the rooting culture medium is prepared from 1 / 2 MS, 1.0 mg / L to 1.4 mg / L of IBA, 0.2 mg / L to 0.4 mg / L of NAA and 0.04 mg / L to 0.07 mg / L of brassinolide. By using the method provided by the invention, the tomato spotted wilf virus detoxification success rate in the tsaoko amomum fruit tissue culture process can be effectively improved.

The invention discloses a cutting seedling rooting agent and a preparation method thereof, and relates to the technical field of garden horticulture, and the cutting seedling rooting agent comprises the following raw materials by weight: 5-15% of a citrus pectin oligosaccharide-spermidine compound, 0.0001-0.0005% of a brassinolide nanoemulsion, 2-5% of proline, 3-8% of sugar alcohol calcium, 5-10% of modified attapulgite, 0.25-0.55% of a surfactant, and the balance of deionized water. After use, the rooting effect is good and the stability is high.

The invention discloses a macadamia nut fruit retention and fruit setting fertilizer based on hormone synergistic regulation and an application method thereof, and relates to the technical field of fertilizers. The fertilizer comprises a first component, a second component, a third component and a fourth component, the first component is prepared from the following raw materials: urea, vermiculite, water-retaining composite gel, humic acid, pseudomonas fluorescens, actinomycetes and decomposed straw; the second component raw materials comprise stimulant, gibberellin, auxin, sodium thiosulfate, mineral oil, polyvinyl alcohol, calcium chloride, potassium silicate, boric acid and brassinosteroid; a third component raw material: a medium-nitrogen low-phosphorus high-potassium compound fertilizer and a medium-trace element fertilizer; and the fourth component comprises the following raw materials: compound sodium nitrophenolate, isopentenyl adenine, glycine betaine, mineral oil and polyvinyl alcohol. The fertilizer disclosed by the invention can provide sufficient water and nutrients in a young fruit stage, reduces the physiological fruit dropping rate in the early stage and the middle and later stages, can promote protein and fat accumulation in a fruit expansion stage and a fruit maturation stage, and reduces the physiological fruit dropping rate in the middle and later stages.

This invention relates to the field of biotechnology, specifically disclosing a method for improving the biocompatibility of poplar trees. PagBEH3b Methods for promoting poplar lignite development and / or increasing poplar height and / or increasing poplar yield through gene expression. This invention involves... PagBEH3b Genes were transferred into poplar trees and overexpressed. PagBEH3b Compared with wild-type, transgenic poplar showed a significant increase in xylem, indicating that the xylem of the brassinolide signaling pathway is a key regulator of this pathway. PagBEH3b It is a key regulatory factor in controlling xylem development in poplar. This invention provides a new option for regulating xylem development and has significant application value in the field of forest tree genetic engineering.

The invention discloses an SlFMO1 gene and application thereof in regulation and control of growth and development of tomato plants, and belongs to the technical field of gene engineering. According to the method, a CRISPR / Cas9 gene editing technology is utilized, firstly, a CRISPR / Cas9-gRNA vector is constructed to accurately edit an SlFMO1 gene in tomatoes, GV3101 agrobacterium tumefaciens is utilized to conduct mediated transformation on the tomatoes, and kanamycin serves as a resistance marker to conduct screening to obtain SlFMO1-deleted mutant tomatoes. Phenotype observation and analysis show that after the SlFMO1 gene is knocked out, the plant height and the plant width of a tomato plant are obviously increased, the apical development is enhanced, the stem diameter of the plant is increased, and the inflorescence spacing is increased, which indicates that the SlFMO1 gene negatively regulates the growth and development of the tomato, and meanwhile, the expression of related genes synthesized by auxin, gibberellin and brassinolide is obviously enhanced. The discovery has important theoretical significance and practical application value for follow-up cultivation of ideal tomato varieties.

The invention relates to a method for promoting centella asiatica seed germination, and belongs to the technical field of medicinal plant cultivation. Aiming at the technical problems of low germination rate and long period of centella asiatica seeds caused by seed coat obstacles and endogenous inhibitors, the method comprises the following steps: firstly, sequentially carrying out mechanical coat breaking, ultrasonic treatment and high-pressure permeation treatment on the seeds to break a physical barrier; mixing the seeds with a wet substrate, carrying out warm lamination at 18-25 DEG C for 25-35 days, then carrying out cold lamination at 2-6 DEG C for 50-70 days, and spraying a KNO3 solution in the cold lamination stage to activate physiological metabolism; after lamination, washing with running water, sequentially soaking with a thiourea solution, carrying out H2O2 treatment for chemical degradation, and carrying out biological adsorption by adopting an adsorbent containing bacillus subtilis and activated carbon; soaking the seeds into a mixed solution containing brassinolide, kinetin and fluridone, and carrying out gradient heating and drying; and finally carrying out germination culture. The method can significantly improve the germination efficiency and uniformity, and is suitable for centella asiatica large-scale seedling raising and medicinal resource development.

The invention provides a plant biostimulant composition for increasing the carotenoid content of fruits and vegetables as well as a preparation method and a use method of the plant biostimulant composition. The plant biostimulant composition is prepared from the following raw materials in parts by weight: 0.01 to 0.1 part of brassinolide (BR), 50 to 200 parts of sodium sulfite (Na2SO3), 1000 to 2000 parts of ammonium polyphosphate (APP), 50 to 200 parts of APP-manganese chelate (in terms of Mn element), 500 to 2000 parts of APP-magnesium chelate (in terms of Mg element) and 25 to 100 parts of organic silicon additive. The plant biostimulant composition is sprayed on the leaf surfaces of fruit trees, so that crop bodies can be stimulated to increase synthesis and storage of carotenoids, the content of the carotenoids in crops is increased, accumulation of flavor substances is increased, and the quality of fruits and vegetables is improved.

The present invention provides a method for improving the survival rate of rice radiation-induced mutagenesis seedlings and / or promoting the growth of rice radiation-induced mutagenesis seedlings, and belongs to the field of agricultural technology. The present invention provides the use of brassinolide in improving the survival rate of rice radiation-induced mutagenesis seedlings and / or promoting the growth of rice radiation-induced mutagenesis seedlings. Radiation treatment of rice seeds to create mutants is one of the important ways to innovate germplasm resources. However, irradiating rice seeds will affect their physiological metabolic activities, resulting in abnormal growth or even death of seedlings, thereby significantly reducing the mutagenesis efficiency. The present invention utilizes the plant growth regulator brassinolide to treat rice seeds at different growth stages after radiation treatment, which can improve the survival rate of induced seedlings, reduce the loss of mutant genes, and thus improve the efficiency of radiation mutagenesis.

This invention relates to compositions and methods for modifying Brassinosteroid Insensitive-1 (BRI1) genes in plants by introducing at least one mutation in a region of the BRI1 gene encoding the kinase domain, optionally to improve yield traits. The invention further relates to a mutated BRI1 gene, a modified BRI1 polypeptide, and plants having increased improved yield traits produced using the methods and compositions of the invention.

The invention discloses a production method for regulating and controlling Yunnan purple garlic test tube bulbs by brassinolide. According to the method, the Yunnan purple garlic rachis is taken as an explant, and efficient production is realized by establishing a technical system of cluster seedling induction, differentiation and growth and test tube bulb formation. The following culture medium conditions are specifically adopted: a cluster seedling induction culture medium is MS + 1 mg.L <-1 > KT + 0.1 mg.L <-1 > NAA + 0.1 mg.L <-1 > BR + 30.0 g.L <-1 > sucrose + 7.5 g.L <-1 > agar, and the pH is 6.2; the test tube bulb induction culture medium is MS + 90 g.L <-1 > sucrose + 6.8 g.L <-1 > agar, and the pH value is 7.5. Under the system, the adventitious bud induction rate of the clustered seedlings and the formation rate of the test-tube bulbs are up to 98.14% or above, the obtained test-tube bulbs meet detoxification standards, each rachis can produce 16.89 bulbs at most, and the bulbs have growing points and germination potential. In addition, the soluble sugar content of the bulbs is reduced by 50.31%, and long-term storage is facilitated. The method provides reliable technical support for detoxification and rapid propagation breeding of the Yunnan purple garlic.

The invention belongs to the technical field of plant genetic engineering and the technical field of soybean breeding, and particularly discloses application of a soybean brassinolide inactivating enzyme encoding gene GmBAS1C in regulation and control of the soybean branch number, the CDS sequence of the soybean brassinolide inactivating enzyme encoding gene GmBAS1C is shown as SEQ ID No.1, the amino acid sequence of the encoded protein is shown as SEQ ID No.2, and the amino acid sequence of the soybean brassinolide inactivating enzyme encoding gene GmBAS1C is shown as SEQ ID No.3. The invention further discloses application of the soybean brassinolide inactivating enzyme encoding gene GmBAS1C in regulation and control of the soybean branch number. The gene is located in an endoplasmic reticulum, and formation and development of lateral branches are promoted by up-regulating expression quantities of genes such as brassinolide synthesis genes CPD, ROT3 and BR6OX2, auxin response genes IAA, GH3 and SAUR and the like. The invention also discloses three homozygous gene editing mutants (KOGmBAS1C-1, KOGmBAS1C-2 and KOGmBAS1C-3) of the GmBAS1C gene, a soybean plant containing the gene editing mutants, and a vector construction method of the soybean plant. The invention further discloses the three homozygous gene editing mutants (KOGmBAS1C-1, KOGmBAS1C-2 and KOGmBAS1C-3) of the GmBAS1C gene. Experiments prove that the number of branches of homozygous gene editing mutant phenotypes of the three GmBAS1C genes is remarkably increased. The invention provides a new gene target, a molecular tool and a breeding material for soybean high-branch breeding, and has important agricultural application value.

The invention discloses a material-saving-free cultivation method and application of eucalyptus tomentosa seedlings. The cultivation method comprises the step of spraying an exogenous hormone aqueous solution in stages in the 3-8-month-old period of the eucalyptus tomentosa seedlings: (1) at the first stage, spraying a gibberellin aqueous solution; (2) a second stage: spraying a brassinolide aqueous solution; and (3) a third stage: spraying a mixed aqueous solution containing brassinolide and abscisic acid. The material-saving-free cultivation method for the eucalyptus tomentosa nursery stock is used for material-saving-free eucalyptus tomentosa cultivation, synchronous regulation and control of trunk height promotion and lateral branch inhibition of the eucalyptus tomentosa nursery stock can be achieved, the trunk cleaning height and the trunk cleaning rate are remarkably improved, and early-stage material-saving-free formation of the eucalyptus tomentosa is promoted.

The invention belongs to the technical field of botanical herbicides, and provides spirobrassinolide as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing isatin, nitromethane, a chiral catalyst and tetrahydrofuran, and performing asymmetric Henry reaction to obtain an intermediate 1; mixing the intermediate 1, methanol and a palladium-carbon catalyst, and performing catalytic hydrogenation reduction reaction to obtain an intermediate 2; mixing the intermediate 2, carbon disulfide, a sodium hydroxide solution and methyl iodide, and carrying out an addition methylation reaction to obtain an intermediate 3; and mixing the intermediate 3, dichloromethane, pyridine and methylsulfonyl chloride, and carrying out a substitution cyclization reaction to obtain the spirobrassinolide. The process route is efficient and excellent in stereoselectivity, the problem of low efficiency of traditional natural product separation is avoided, and the prepared spirobrassinolide is high in weed inhibition activity, has a remarkable inhibition effect on growth of gramineous weed millet and broadleaf weed chenopodium album and has the potential of becoming a novel plant source herbicide.

The invention provides brassinosterols and application of an inhibitor of the brassinosterols in regulating and controlling the de novo regeneration of dicotyledonous plant root organs, and belongs to the technical field of plant growth regulation and control. According to the invention, brassinosteroid (BR) related mutants are used as materials, and the function of BR in the de novo regeneration process of roots is explored through an in-vitro leaf root regeneration induction method. The result shows that the BR signal can inhibit the root regeneration process, based on this, the invention provides the application of the brassinosteroid synthesis inhibitor in promoting generation of root organs of dicotyledonous plant in-vitro tissue and the application in dicotyledonous plant in-vitro culture, the brassinosteroid synthesis inhibitor is exogenously added, and the yield of the brassinosteroid synthesis inhibitor is improved. The rooting process of the dicotyledonous plant in-vitro leaves can be promoted, the in-vitro culture process is accelerated, and the application value is high.

The application discloses a large-scale cutting of ginkgo and a cutting method thereof, and the cutting method comprises the following steps: cutting and dwarfing pruning a 4-5 year old ginkgo mother plant, pruning the lateral branches of the cut mother plant for multiple times, spraying a plant growth regulator after each pruning to promote the development of the lateral branches of the mother plant, cutting the branches on the lateral branches of the mother plant as cuttings, and inserting the cuttings into a substrate after treating the cuttings with a plant growth regulator. The method adopts the cutting and multiple pruning to promote the sprouting of the plant and generate the juvenile branch, greatly increases the number of cuttings, sprays 6-benzyladenine or brassinolide to improve the quality and quantity of the cuttings, and further uses methyl jasmonate, naphthalene acetic acid and uniconazole to jointly treat the cuttings to significantly improve the rooting rate of the cuttings. The method can be used for cultivating the leaf ginkgo and the ginkgo mother plant with excellent characteristics, significantly shortens the formation time of the cutting callus and adventitious roots, is simple and easy to operate, is suitable for large-scale cutting, and has popularization value in the asexual propagation of the ginkgo clone.

The present invention relates to the field of genetic engineering technology, and in particular to the use of the GmDET2-1 protein and its encoding gene in increasing the oil content of soybean seeds. The present invention is the first to discover that the soybean brassinosteroid synthase GmDET2-1 protein or its encoding gene can regulate the oil content of plant seeds. This has high practical application value in plant variety improvement or breeding.

The application provides application of an XTH gene in promotion of grape fruit hardness formation by brassinosteroids, finds that brassinosteroids (BR) can mediate synthesis of hemicellulose in grape fruits and thus significantly improve the hardness of the grape fruits by treating the grape fruits with the brassinosteroids at a veraison stage, finds two target genes VvXTH25 and VvXTH31 capable of regulating the hardness of the grape fruits through whole genome correlation analysis and transcriptome analysis, and determines the key genes and mechanism for regulating the hardness of the grape fruits, which regulates the hardness of the grape fruits by regulating the content of hemicellulose, has the action of providing the basis and means for regulating the texture of the grape fruits and improving the storage and transportation capacity, and provides a brand-new way for truly regulating the hardness of the grape fruits.

The invention discloses an application of 24-Epi brassinolide in promoting the development of female germline stem cells. Belongs to the technical field of reproductive biology. Female germline stem cells (FGSCs) are important cell models for studying the development of germ cells, however, the regulation and control mechanism of natural products on the development of the FGSCs still needs to be deeply explored. The invention aims at disclosing the regulation and control effect of 24-Epi brassinolide (24-Epibrassinolide, EBR) on FGSCs development and a molecular mechanism of the 24-Epi brassinolide on the FGSCs development by utilizing an FGSCs in-vitro development system and combining LongRNA-seq and RIC-seq technologies, and a new strategy is provided for treatment of infertility and optimization of a clinical treatment scheme.

A biochar-seed gel for vegetation rehabilitation in saline-alkali soil, a preparation method thereof, and an application thereof are provided. The biochar-seed gel includes a water-retaining hydrogel, effective nutrient components, and a structural maintenance agent, where the water-retaining hydrogel is polyacrylamide; the effective nutrient components include Bacillus subtilis inoculant, brassinolide, chitosan oligosaccharide, polyaspartic acid, and compound fertilizer; and the structural maintenance agent includes biochar, clay, chitosan, and Gleditsia sinensis powder. The biochar-seed gel forms a microenvironment on the soil surface, mitigating stress caused by high-salinity soil conditions. The gel exhibits strong water-retention capacity, effectively maintaining moisture to support seed and seedling growth while alleviating water deficiency resulting from soil compaction.

The invention relates to the technical field of analysis and detection, in particular to a molecular imprinting sensor for in-situ detection of brassinolide in plant leaves and a preparation method of the molecular imprinting sensor. The molecular imprinting sensor for detecting brassinolide is prepared by assembling a 4-MPBA-Fc-GO-metal polynitrogen azole framework composite material and MIP on the surface of an electrode layer by layer, and high-selectivity and high-sensitivity detection of target molecules is realized. The technology can effectively reduce the detection cost, simplify the operation process and improve the performance of the sensor. By using the molecular imprinting sensor, brassinolide in plant leaves can be analyzed in vivo and in situ, rapid and reliable quantitative analysis can be realized in complex samples, and the molecular imprinting sensor has a wide application prospect.

The invention belongs to the technical field of agricultural biology and seed engineering, and relates to a growth-promoting stress-resistant rape seed pelleting agent and application. The pelleting agent has the functions of growth promotion, disease resistance (clubroot), pest killing and stress resistance (stain resistance and cold resistance), and is formed by scientifically compounding an inert filler, a composite growth promotion component (including melatonin, naphthylacetic acid and abscisic acid), a bactericide (fluazinam or trichoderma), a broad-spectrum insecticide (70% thiamethoxam), a plant growth regulator (brassinolide or selenium medicament), an adhesive and an auxiliary agent. When an optimal formula of the composite preparation of fluazinam (the ratio of pesticide to seeds is 1: 100) and brassinolide is used, the cost is only about 0.67 yuan per mu, the yield can be increased by about 14 kilograms, the stable net income increase of 85-219 yuan per mu can be realized, and the input-output ratio is as high as 1: 126-1: 337. The invention provides an efficient, safe and comprehensive seed treatment scheme, and has important application value for guaranteeing stable and high yield of rape.

The invention relates to a method for promoting yield increase and quality improvement of peony seeds, which comprises the following steps: after pollination of peony is completed, spraying an exogenous hormone gibberellin GA3 solution or brassinolide BR solution to fruit pods of peony plants, and spraying to the fruit pods to enable liquid beads to drip. By means of the method, generation and growth of peony seeds are promoted, the number of normal peony seeds is greatly increased, the number of abortive seeds is reduced, and after gibberellin GA3 or brassinolide BR is sprayed for treatment, the number of the normal seeds is increased by 50% or above and can reach 2-3 times; the number of abortive seeds is reduced by more than 20%; the size of the peony seeds is increased, the length and width of the seeds are obviously increased, the problem of environmental pollution easily caused by massive fertilization is effectively avoided, the production cost is reduced, and the method is easy to operate and popularize and has important significance for promoting the industrialization development of the peony.

The invention relates to the technical field of saline-alkali soil improvement, in particular to a saline-alkali soil conditioner. The invention provides a saline-alkali soil conditioner. The saline-alkali soil conditioner is prepared from the following components in parts by mass: 10 to 14 parts of improving fungicide, 30 to 40 parts of fermented corn steep liquor, 1 to 3 parts of brassinolide, 0.6 to 1 part of betaine, 2 to 3 parts of potassium fulvate, 3 to 5 parts of polyaspartic acid, 3 to 5 parts of alginic acid extracting solution, 0.4 to 0.6 part of alkyl polyglycoside, 0.1 to 0.3 part of boric acid, 0.8 to 1.2 parts of potassium sulfate, 0.2 to 0.3 part of zinc chloride, 0.1 to 0.3 part of ammonium molybdate and 4 to 6 parts of calcium sulfate. According to the saline-alkali soil conditioner disclosed by the invention, through the synergistic effect of the compound microbial flora and the organic-inorganic components, the saline-alkali stress of soil is effectively reduced, and the growth of crops is promoted.

The present invention discloses the use of brassinolide for increasing the sakurain content in rice. This study first discovered that brassinolide can be used to increase sakurain content in rice and hypothesized the mechanism, suggesting that BL treatment induces the expression of the sakurain biosynthesizing enzyme OsNOMT, thereby increasing sakurain content. The present invention is simple to implement and has significant effects, providing a powerful reference for increasing sakurain content in rice and improving rice immunity.

The invention belongs to the technical field of molecular genetics, and particularly relates to an application of a ZmNAL4 gene or an encoded protein thereof in regulation and control of leaf morphology and plant height of a plant. The analysis of the expression mode of the flavin monooxygenase gene ZmNAL4 in amphiphilic and transgenic plants shows that the leaf width of an overexpression strain is increased by 13.2% and the plant height is increased by 14.8% compared with that of a gene editing strain, and the leaf area and the plant height of an arabidopsis overexpression plant are obviously higher than those of a wild plant, so that the ZmNAL4 has a positive regulation effect on the leaf width; interaction protein analysis identifies that density-tolerant response factors Zmfdx1 and Zmfdx2 and leaf included angle candidate genes ZmPDHE1 and ZmNAL4 interact with each other; plant endogenous hormone analysis shows that the ZmNAL4 regulates and controls the leaf form and the plant height by influencing an auxin signal and a brassinolide path; gene resources and theoretical basis are provided for research on density-tolerant plant types with reasonable leaf widths of corn, and the method has important significance on density-tolerant high-yield breeding.