Method for promoting single-needle algae accumulated grease by nitrogen deficiency combined with strigolactones

A technology of strigolactone and monospermia, which is applied in the field of biodiesel, can solve the problems of long growth cycle of microalgae algae cells, influence on growth and oil accumulation, and cannot be applied on a large scale, and is beneficial to the growth of microalgae. and the accumulation of oil and fat content, the effect of promoting the accumulation of oil and fat, and the effect of promoting the accumulation of oil and fat content

Inactive Publication Date: 2019-12-24
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, autotrophic culture is mainly used, but the culture method of microalgae has the reasons of long algae cell growth cycle, low yield, easy to infect bacteria and high harvesting cost, so it cannot be applied on a large scale; at the same time, in microalgae culture During the process, in addition to the influence of culture methods, light, temperature, metal ions, abiotic stress and plant hormones also have significant effects on growth and oil accumulation

Method used

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  • Method for promoting single-needle algae accumulated grease by nitrogen deficiency combined with strigolactones
  • Method for promoting single-needle algae accumulated grease by nitrogen deficiency combined with strigolactones
  • Method for promoting single-needle algae accumulated grease by nitrogen deficiency combined with strigolactones

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Prepare a nitrogen-deficient autotrophic BG-11 medium as the basic medium for oleaginous microalgae, adjust the pH value to 6.8-7.0; sterilize under high pressure and high temperature for 20 minutes, insert Monospiritoleum oleaginum, and control the algae cell concentration at 0.7 g / L, without adding SLs, the light culture temperature is 25°C, the light intensity is 3500 lux, the shaker speed is 150r / min, and light shake flask culture is carried out. The maximum oil content of the monospora oleaginous cultured in this example reached 46.18%; the maximum biomass reached 0.86g / L.

Embodiment 2

[0027] Prepare the self-supporting BG-11 basic medium as the basic medium for oleaginous microalgae, adjust the pH value to 6.8-7.0; sterilize at high pressure and high temperature for 20 minutes, insert the oleaginous monospora, and control the algae cell concentration at 0.7g / L, SLs were added to the culture medium so that the final SLs concentration was 1 μM, the light culture temperature was 25° C., the light intensity was 3500 lux, and the shaker speed was 150 r / min, and light shake flask culture was carried out. The maximum oil content of Monopodella oleaginum cultivated in this control example reached 48.76%; the maximum biomass reached 0.91g / L.

Embodiment 3

[0029] Prepare a nitrogen-deficient autotrophic BG-11 medium as the basic medium for oleaginous microalgae, adjust the pH value to 6.8-7.0; sterilize under high pressure and high temperature for 20 minutes, insert Monospiritoleum oleaginum, and control the algae cell concentration at 0.7 g / L, SLs were added to the medium so that the final SLs concentration was 1 μM, the light culture temperature was 25°C, the light intensity was 3500 lux, and the shaker speed was 150 r / min, and light shake flask culture was carried out. The maximum oil content of the monospora oleaginous cultured in this example reached 53.71%; the maximum biomass reached 0.90 g / L.

[0030] The culture solution obtained in Comparative Example 1 and Examples 1 to 3 was enriched by centrifugation (3500r / min, 10min), washed repeatedly with distilled water twice, then freeze-dried and weighed; add freeze-dried algae powder with 2 times the mass of quartz sand and grind for 20min Afterwards, the oil was extracted w...

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Abstract

The invention discloses a method for promoting single-needle algae accumulated grease by nitrogen deficiency combined with strigolactones. Single-needle algae is cultivated through two stages. The method for promoting the single-needle algae accumulated grease by the nitrogen deficiency combined with the strigolactones comprises the following steps that firstly glucose is used as an organic carbonsource to heterotrophically cultivate the single-needle algae to a logarithmic phase later period to collect algae cells; then autotrophy anazotic BG-11 is used as an inducing culture medium of a second stage to renewedly suspend the algae cells to be used as inducing algae liquid; 1mmol/L strigolactones (SLs) mother solutions are prepared by acetone, the strigolactones (SLs) mother solutions areadded into the inducing algae liquid to dilute the strigolactones (SLs) mother solutions to the concentration of 1[mu]mol/L, and the algae liquid is placed in a shaking table for lighting cultivating; and the algae cells cultivated to a stable phase are enriched by a centrifugal separation method, grease is extracted, and methyl esterification is carried out to prepare biodiesel. The method for promoting the single-needle algae accumulated grease by the nitrogen deficiency combined with the strigolactones is simple and practicable, can greatly shorten the cultivated time of the oil-producingsingle-needle algae, obviously increases the grease yield, and thus, greatly improves the efficiency of microalgae for producing the biodiesel.

Description

technical field [0001] The invention belongs to the technical field of biodiesel, and in particular relates to a method for promoting the accumulation of oil in Monopodella algae in combination with nitrogen deficiency and strigolactone. Background technique [0002] In recent years, problems such as environmental pollution and energy shortage caused by excessive use of fossil energy have become increasingly serious. As an ideal substitute for fossil energy, biodiesel has attracted widespread attention. It can be prepared by transesterification of animal and vegetable oils. Its performance indicators Good control, good stability. Biodiesel, fatty acid methyl ester, is an oxygen-containing clean fuel, which is formed by transesterification of renewable oils such as rapeseed oil, soybean oil, recycled cooking oil, animal oil, microbial oil and short-chain alcohols mixed lipids. As a high-quality diesel substitute, biodiesel is a renewable, biodegradable, stable and non-toxic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/38C12P7/64C12R1/89
CPCC12N1/12C12N1/38C12P7/6463C12P7/649Y02E50/10
Inventor 余旭亚余春丽宋雪婷
Owner KUNMING UNIV OF SCI & TECH
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