Method for improving tea seedling field fungus rooting cuttage breeding effects

A technology of mycorrhizalization and tea seedlings, applied in the agricultural field, can solve problems such as poor breeding effects, and achieve the effects of low cost, low labor intensity and simple operation

Active Publication Date: 2019-06-07
GUIZHOU TEA RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for improving the effect of mycorrhized cutting propagation of tea seedlings

Method used

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  • Method for improving tea seedling field fungus rooting cuttage breeding effects
  • Method for improving tea seedling field fungus rooting cuttage breeding effects
  • Method for improving tea seedling field fungus rooting cuttage breeding effects

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Experimental program
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Effect test

Embodiment 1

[0022] 1) Bacteria preparation:

[0023] a) Prepare a matrix with yellow sand and river sand at a ratio of 1:1 and sterilize it by irradiation (12kGy) for planting white clover to cultivate high-efficiency AMF strains.

[0024] b) Select white clover with well-developed root system as the host, scrub the clover seeds, soak them in 0.3% potassium permanganate solution for 3-4 hours, wash them with clean water, soak them in warm water at 20-30°C for 1 day, and drain them;

[0025] c) the treated host seeds are applied to the matrix respectively with the AMF bacterial agent, and the water used during the cultivation is sterile water;

[0026] d) After 3-4 months of inoculant culture, remove the host plant, mix the substrate and granulate it for later use.

[0027] 2) Seedbed establishment:

[0028] a) The test site was selected at the Guiyang tea garden base of Guizhou Tea Research Institute, and tea trees were planted for a long time in the early stage of the test;

[0029] b...

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Abstract

The invention discloses a method for improving tea seedling field fungus rooting cuttage breeding effects. According to the method, AMF (arbuscular mycorrhizal fungi) efficient strain rhizophagus intraradices is applied into a cutting seedbed in advance, and strigolactones is sprayed after cutting is completed, so that the tea seedling field fungus rooting cuttage breeding effects are improved. Test results show that tea seedlings are bred by the method, the tea seedling field fungus rooting cuttage breeding effects can be effectively improved, infection rate of AMF for the tea seedlings is increased, the germination rate, the callus occurrence rate and the survival rate of the sea seedlings are increased, growth indexes such as stem height, root length and dry and fresh weight of the teaseedlings are facilitated, and the method has the advantages that the method is simple to operate, low in labor intensity and suitable for popularization and application in actual production, investment increase is less and the like.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a method for improving the effect of mycorrhized cutting propagation of tea seedlings in the field. Background technique [0002] Tea tree (Camellia sinensis (L.) O. Kuntze) belongs to the Camellia family Camellia, is a perennial evergreen woody plant, and is one of the important economic crops in my country. At present, my country's tea planting area continues to expand, with an annual increase of more than 50,000 hectares. The main breeding method of domestic tea trees is the cutting propagation method, that is, when cutting, select a 2.5-3cm semi-lignified short ear with a complete mature leaf and a full axillary bud. Mature and complete leaves are used for ear cuttings. Compared with the traditional sowing seedlings, this method has the advantages of retaining the excellent traits of the mother ear and facilitating the standardized production and management of tea gar...

Claims

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Application Information

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IPC IPC(8): A01G2/10A01G17/00
Inventor 高秀兵郭灿周玉锋赵华富陈瑶张金峰杨肖委
Owner GUIZHOU TEA RES INST
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