Rice tillering associated protein, encoding gene and use thereof
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A technology for tiller-related proteins and coding genes, applied in rice tiller-related proteins and their coding genes and applications, to achieve the effect of regulating utilization efficiency
Inactive Publication Date: 2009-11-25
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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Embodiment 1
[0048] Embodiment 1, the discovery of protein and its coding gene
[0049] The seeds of the rice (Oryza sativa L.) dwarf multi-tiller mutant dwarf27 (d27) and the wild-type Shiokari were cultivated according to the above-mentioned field cultivation methods, and the seedlings had the following morphology: figure 1 Shown (A represents wild-type Shiokari, B represents dwarf multi-tiller mutant d27). Then the leaves were taken for DNA extraction.
[0050] Extraction of rice genomic DNA:
[0051] Using improved CTAB method (Mou Z, He Y, Dai Y, et al.Deficiency in fatty acidsynthase leads to premature cell death and dramatic alterations in plantmorphology.The Plant Cell.2000,12,405-418.) from rice leaves Extract genomic DNA. Take 100mg of rice leaves, freeze them with liquid nitrogen, grind them into powder in a small mortar with a diameter of 5cm, transfer them to a 1.5ml centrifuge tube to extract DNA, and dissolve the obtained DNA precipitate in 100μl MQ H 2 O middle.
[005...
Embodiment 2
[0062] Embodiment 2, function and application of gene
[0063] 1. Gene preparation
[0064] Extract the mRNA of the wild-type variety Shiokari seedlings, reverse transcribe to obtain cDNA, use primers to perform PCR amplification on D27F / D27R, sequence the product, and obtain the cDNA sequence of the D27 gene in the wild-type variety, and its nucleotide sequence is shown in the sequence table As shown in Sequence 2, the amino acid sequence of the encoded protein is shown in Sequence 4 in the sequence listing.
[0065] Extract the mRNA of d27 mutant seedlings, reverse transcribe to obtain cDNA, use primers to perform PCR amplification on D27F / D27R, sequence the product, and obtain the cDNA sequence of D27 gene in mutant d27, and its nucleotide sequence is as shown in the sequence list 3, the amino acid sequence of the encoded protein is shown in sequence 5 in the sequence listing.
[0066] D27F: 5'-ATGGAGACCACCACGCTTGTGC-3' (SEQ ID NO: 6),
[0067] D27R: 5'-TCAGATGGAGCAATTCA...
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Abstract
The present invention discloses a rice tillering associated protein, the encoding gene and the use thereof. The protein is selected from the following proteins (a) and (b): (a) a protein which is composed of the amino acid sequence represented by the sequence 4 in the sequence table; (b) a protein which is associated with the plant tillering and is derivate from the protein (a) through substituting and/or deleting and/or adding one or a plurality of amino-acid residues to the amino acid sequence represented by the sequence 4 in the sequence table. The transgene complementation test proves that the protein D27 coded by the gene participates in the synthesizing of the strigolactone and regulates the plant tillering or the branch stem developing. Therefore, the genetic engineering technology can be used for regulating the tillering trait of the rice, regulating the nutrition using efficiency of the plant and effectively controlling the destructive parasitic weed. The D27 gene has important application value in the aspect of researching the beneficial fungus association and the destructive parasitic weed in the agriculture and natural ecosystem.
Description
technical field [0001] The invention relates to a rice tiller-related protein, its coding gene and application. Background technique [0002] The branches that grow on the basal internodes of rice are generally called tillers, which are a specific type of branches in rice. Tillering is an important agronomic trait in rice production. On the one hand, the number of effective tillers per plant determines the number of panicles per unit area, and the number of panicles determines the yield per unit area; on the other hand, the number of tillers per plant is an important factor in the composition of the plant structure. The ideal plant structure has Help ventilation and light transmission, improve photosynthetic efficiency, absorb and distribute mineral nutrients reasonably, thereby promoting effective tillering and inhibiting ineffective tillering. In production practice, on the one hand, according to the law of tiller growth and development, appropriate cultivation measures ...
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