Biological periosteum repair material and preparation method thereof

A technology of mucosal layer and submucosa layer, applied in tissue regeneration, medical science, prosthesis, etc., can solve problems such as biological safety and practicability without too much consideration, so as to avoid cytotoxicity, improve biological safety, and improve The effect of biocompatibility

Active Publication Date: 2019-03-22
SUMMIT GD BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these studies are still in the early research stage, and the issues of biosafety and practicality have not been considered too much
[0006] At present, in view of the important role of periosteum in bone repair, and there is no effective product for repairing periosteum to treat bone defects

Method used

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  • Biological periosteum repair material and preparation method thereof

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Effect test

Embodiment 1

[0026] This embodiment provides an acellular matrix material for submucosa tissue of pig small intestine and a preparation method thereof. The preparation method includes the following steps:

[0027] Step 1. Take the jejunum segment of the pig small intestine, clean it and cut it radially, and rinse it repeatedly with PBS solution to remove contaminants.

[0028] Step 2. Physically remove the mucosa, use a wooden scraper to carefully and evenly scrape the mucosa in the inner layer of the SIS membrane.

[0029] Step 3. Physically remove the muscle membrane and serous membrane, and use a wooden scraper and scalpel together to remove the muscle membrane and serous membrane of the outer layer of the SIS membrane.

[0030] Step 4. Prepare 0.18% (mass fraction) peroxyacetic acid solution, 0.18% (mass fraction) sodium hydroxide solution, and 80% acetone solution;

[0031] Then, the SIS membrane with the mucosal layer, muscle membrane layer and serosal layer removed is first immersed in perac...

Embodiment 2

[0037] This embodiment provides an acellular matrix material for submucosa tissue of pig small intestine and a preparation method thereof. The preparation method includes the following steps:

[0038] Step 1. Take the jejunum segment of the pig small intestine, clean it and cut it radially, and rinse it repeatedly with PBS solution to remove contaminants.

[0039] Step 2. Physically remove the mucosa, use a wooden scraper to carefully and evenly scrape the mucosa in the inner layer of the SIS membrane.

[0040] Step 3. Physically remove the muscle membrane and serous membrane, and use a wooden scraper and scalpel together to remove the muscle membrane and serous membrane of the outer layer of the SIS membrane.

[0041] Step 4. Configure 0.25% (mass fraction) peroxyacetic acid solution, 0.25% (mass fraction) sodium hydroxide solution, and 85% acetone (mass fraction) solution;

[0042] Then, the SIS membrane with the mucosal layer, muscle membrane layer and serosal layer removed is first...

Embodiment 3

[0048] This embodiment provides an acellular matrix material for submucosa tissue of pig small intestine and a preparation method thereof. The preparation method includes the following steps:

[0049] Step 1. Take the jejunum segment of the pig small intestine, clean it and cut it radially, and rinse it repeatedly with PBS solution to remove contaminants.

[0050] Step 2. Physically remove the mucosa, use a wooden scraper to carefully and evenly scrape the mucosa in the inner layer of the SIS membrane.

[0051] Step 3. Physically remove the muscle membrane and serous membrane, and use a wooden scraper and scalpel together to remove the muscle membrane and serous membrane of the outer layer of the SIS membrane.

[0052] Step 4. Configure 0.15% (mass fraction) of peracetic acid solution, 0.15% (mass fraction) of sodium hydroxide solution, and 75% (mass fraction) of acetone solution;

[0053] Then, the SIS membrane with the mucosal layer, muscle membrane layer and serosal layer removed is...

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Abstract

The invention relates to a biological periosteum repair material and a preparation method thereof, and particularly relates to an acellular dermis matrix material and a preparation method and application thereof to periosteum repair. The preparation method comprises: (1) taking a jejunum segment of a pig small intestine, cleaning up, radially cutting open, removing a mucous layer, a myolemma layerand a serosal layer and obtaining a pig small-intestine mucous lower-layer tissue membrane; (2) sequentially carrying out the following processing on the obtained pig small-intestine mucous lower-layer tissue membrane: firstly, placing in peracetic acid solution to soak and cleaning up; then placing in sodium hydroxide solution to soak and cleaning up; finally, placing in acetone solution to soak, cleaning and obtaining a pig small-intestine mucous lower-layer tissue acellular matrix. The biological periosteum repair material is high in biological safety, low in immunogenicity and cytotoxicity, good in biocompatibility, can promote multiplication of cells adhered to the biological periosteum repair material, is applicable to use as a biological repair material and is particularly suitablefor periosteum repair.

Description

Technical field [0001] The invention relates to the technical field of tissue repair materials, relates to a biological type periosteal repair material and a preparation method thereof, in particular to an acellular matrix material, a preparation method thereof, and application in periosteal repair. Background technique [0002] Periosteum is composed of microvascularized dense connective tissue, which is tightly covered on the outer surface of cortical bone by Sharpey’s fibers, forming a thin and tough membrane. Periosteum is a highly vascularized tissue that contains osteogenic and chondrogenic progenitor cells and other related biologically active factors. It is described as a better vascularized "bone / cartilage organ", which provides blood circulation for bone, and is considered It is the "umbilical cord" of the bone. Peeling off the periosteum usually destroys the arterial nutrient supply of the bone. In terms of tissue structure, the periosteum is divided into two differen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
CPCA61L27/3633A61L27/365A61L27/3687A61L2430/02A61L2430/40
Inventor 凌友杨景徐斌林祥贵
Owner SUMMIT GD BIOTECH
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