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Staphylococcus caprae for degrading phenol

A staphylococcus, phenol technology, applied in the direction of bacteria, microbial-based methods, water/sludge/sewage treatment, etc., can solve the problems that there are no related reports on phenol research, and achieve good economic value and application prospects

Active Publication Date: 2017-09-05
INNER MONGOLIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few reports on the degradation of pollutants by Staphylococcus. Some studies have shown that Staphylococcus can degrade phenol by more than 80% under the same conditions in 36 hours in a non-salt environment, but the degradation of phenol in a high-salt environment There are no relevant reports in the study

Method used

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  • Staphylococcus caprae for degrading phenol
  • Staphylococcus caprae for degrading phenol
  • Staphylococcus caprae for degrading phenol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The isolation and identification of embodiment 1 Staphylococcus CL

[0025] 1. Isolation of Staphylococcus CL

[0026] a. Dissolve 1-5g of collected solid samples (salt lake sludge, submarine sludge, high-salt wastewater treatment sludge, etc.) or 500ml of liquid samples (salt lake water, seawater, high-salt wastewater, etc.) in 10-50ml Liquid medium (10g MgSO 4 ·7H 2 O, 0.2g CaCl 2 2H 2 O, 2-5g KCl, 2.5g tryptone, 10g yeast extract, NaCl15%, distilled water were added to 1000ml. Adjust the pH to about 7.2, sterilize at 121°C for 15 minutes), shake at 80-160 rpm for 30-120 minutes at room temperature, take it out and let it stand for 20-40 minutes, draw 100 μL with a pipette, and spread evenly on the solid medium for culture Place on a flat plate (obtained by adding 2% agar to the above-mentioned liquid medium), and place it in a constant temperature incubator at 28°C for static culture.

[0027] b. When visible colonies are grown, use an inoculation loop to pick ...

Embodiment 2

[0037] The performance test of the degradation phenol of embodiment 2 Staphylococcus CL

[0038] The performance of staphylococcus CL on the degradation of phenol in phenol-containing medium was detected by high performance liquid chromatography. (medium is 10g MgSO 4 ·7H 2 O, 0.2g CaCl 2 2H 2 O, 2-5g KCl, 2.5g tryptone, 10g yeast extract, NaCl 5%, distilled water were added to 1000ml. Adjust the pH to about 7.2, sterilize at 121°C for 15 minutes, and add phenol according to the following experimental requirements)

[0039] 1. The growth of strain CL and the degradation characteristics of phenol

[0040] Under the optimal growth and degradation conditions, the growth and phenol concentration curves of the strain CL are as follows: figure 2 shown. The strain CL enters the logarithmic growth phase after a stagnation period of about 12 hours. During this period, the strain grows almost linearly with time, and the strain is in a stable growth period close to 40 hours and l...

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Abstract

The invention provides Staphylococcus caprae and application thereof. The bacterial strain is named CL, and the collection number of the bacterial strain is CCTCC NO:M2016305. By adopting the staphylococcus caprae, phenol in 200mg / L liquid culture medium of phenol (10g of MgSO4.7H2O, 0.2g of CaCl2.2H2O, 2 to 5g of KCl, 2.5g of tryptone, 10g of yeast extract, 5 percent of NaCl and distilled water are added to 1,000ml, pH is adjusted to be about 7.2, and sterilization is performed at the temperature of 121 DEGC for 15 minutes) can be degraded by 90 percent or more within 36 hours. The Staphylococcus caprae CL disclosed by the invention has a higher phenol degrading capability, can be applied to biological repair of phenol-polluted environments, and has higher economical value and a better application prospect.

Description

technical field [0001] The invention relates to the technical field of microorganisms and biodegradation, in particular to a staphylococcus and its application in degrading phenol. Background technique [0002] Phenol and its derivatives are highly toxic to humans, animals and plants, and have been included in the list of environmental priority pollutants. Phenolic wastewater mainly comes from industries such as papermaking, oil refining, synthetic fiber, synthetic rubber, and pesticides, and is the main pollutant in industrial wastewater. In addition to phenolic pollutants, these phenolic wastewater often contain a large amount of salt, making it a high-salt phenolic wastewater, which will cause serious pollution to soil, surface water and groundwater when discharged into the environment. Due to the inhibitory and toxic effects of high-salt environment on the growth of microorganisms, conventional biodegradation technology has a bottleneck problem in the treatment of such ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34A62D3/02C12R1/44C02F101/34A62D101/28
CPCA62D3/02A62D2101/28C02F3/34C02F2101/34C12N1/02C12N1/20C12N1/205C12R2001/01C12R2001/44
Inventor 许继飞赵吉宋晓雪武琳慧刘崎峰王川包智华
Owner INNER MONGOLIA UNIVERSITY
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