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Bacillus siamensis capable of being used for repairing concrete cracks, microbial inoculum and application

A Bacillus, concrete technology, applied in the field of microorganisms, can solve problems such as expensive, staying, and small cracks that cannot be repaired

Active Publication Date: 2021-01-08
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Manual repair (such as surface sealing repair method, joint glue injection repair method, etc.) is relatively expensive on the one hand, and on the other hand, the current manual repair method mainly stays on the surface of the concrete structure and in the case of relatively wide cracks, the internal Microcracks and tiny cracks invisible to the naked eye remain irreparable

Method used

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  • Bacillus siamensis capable of being used for repairing concrete cracks, microbial inoculum and application
  • Bacillus siamensis capable of being used for repairing concrete cracks, microbial inoculum and application
  • Bacillus siamensis capable of being used for repairing concrete cracks, microbial inoculum and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032]Example 1: Screening and identification of strains adapted to the preparation of concrete self-repairing bacteria

[0033]1) Take 1g of soil sample from the ancient canal on the Chaohui campus of Zhejiang University of Technology and add it to a sterilized Erlenmeyer flask with small glass beads and 100mL sterile water, shake at 30℃, 200r / min for 30min, and then stand for 10min. , To remove the bottom sediment to obtain a soil suspension. Place the soil suspension in water at 100°C for heating in a boiling water bath for 3-5 min to obtain a soil sample stock solution.

[0034]Perform a gradient dilution of the original soil sample solution in an ultra-clean bench with a concentration gradient of 10-1, 10-2, 10-3, 10-4, 10-5, 10-6Draw 200μL of the bacterial suspension of each concentration and inoculate it on a plate with a selection medium for urease-producing strains, seal the plate with a parafilm and invert it, place it in a constant temperature incubator at 37°C for 24 hours, an...

Embodiment 2

[0043]Example 2: The effect of different conditions on the urease activity of strain ZJB20040

[0044]1) Thermal stability

[0045]Due to the exothermic heat of the hydration process during concrete pouring, the temperature will rise to above 40°C, so the strain ZJB20040 suspension with a concentration of 10 mg / mL was treated in a water bath at 40, 45, 50, 55, and 60°C for 60 minutes. After cooling, count the stained bacterial suspension with a hemocytometer to calculate the ratio of live cells to dead cells. The survival rate of the strain ZJB20040 below 60°C remained above 93%, indicating that the strain ZJB20040 has better thermal stability.

[0046]2) Alkali stability

[0047]Because the hydration process in concrete pouring will make the pH between 12-13, the strain ZJB20040 with a concentration of 10mg / mL is adjusted to 12.5 with 1mol / L sodium hydroxide, and the pH is adjusted to 12.5 after standing for 24h , The solution was filtered through a 0.22μm microporous membrane, and the pH valu...

Embodiment 3

[0048]Example 3: Influence of different inosine concentrations on the spore germination rate of strain ZJB20040

[0049]The strain ZJB20040 was inoculated into a spore fermentation medium with a bottled volume of 200 mL, and placed in a constant temperature culture shaker at 30° C. for shaking culture at 150 r / min for 12 hours. The fermentation broth was centrifuged at 8000r / min for 10min at 4°C to obtain the bacteria. The cells obtained by centrifugation are washed 6-8 times with deionized water to prepare a spore suspension.

[0050]Add glucose, fructose, alanine, glutamic acid, glycine, histidine, lysine, threonine, and inosine to the prepared spore germination basal medium, and make the final concentration all 10g / L, the spore germination basal medium without germination agent is used as a blank control. Take the prepared spore suspension and add it to a 96-well plate containing spore germination medium. The volume ratio of the two is 1:4, and the OD is measured after gently mixing.4...

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Abstract

The invention discloses bacillus siamensis capable of being used for repairing concrete cracks, a microbial inoculum and application. The bacillus siamensis is named as bacillus siamensis, the strainnumber of the bacillus siamensis is ZJB20040, and the preservation number of the bacillus siamensis is CCTCC NO: M 2020276. The bacillus siamensis ZJB20040 provided by the invention has a preservationnumber of CCTCC NO: M2020276, has very good thermal stability and acid-base stability, can adapt to environments with different pH values when being used for repairing concrete cracks, and has good potential and application prospect when being used for preparing a biological repairing preparation for self-repairing the concrete cracks.

Description

Technical field[0001]The present invention relates to the technical field of microorganisms, in particular to a Bacillus siamese, an inoculum, and applications that can be used for concrete crack repair.Background technique[0002]Building materials such as concrete and stone are often subjected to various physical, chemical, biological and other factors during their service period, so there are usually micro-cracks inside. This phenomenon is common in the field of civil engineering. Although the existence of micro-cracks will not directly affect the strength and structural integrity of the concrete, it is easy to cause corrosive gases and water to enter the internal structure of the concrete, thereby affecting the durability and water tightness of the concrete. Especially in a humid environment, the existence of micro-cracks easily exposes the internal structure such as steel bars to the external environment and accelerates the corrosion rate of the overall structure. Artificial repa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C04B24/14C12R1/07
CPCC12N1/20C04B40/0039C12R2001/07C12N1/205C04B24/14C04B24/00C04B22/124C04B24/126C04B2103/0001Y02W30/91
Inventor 柳志强钱媛媛程峰徐建妙王远山贾东旭郑裕国
Owner ZHEJIANG UNIV OF TECH
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