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Method for screening dissimilatory Fe(III)-reducing bacteria

A technology of dissimilatory iron and bacteria, applied in the field of environmental microorganisms, can solve the problems such as hindering the screening of dissimilatory iron reducing bacteria, long screening period, expensive equipment, etc., and achieve the effect of simple and easy screening period and reducing screening cost.

Inactive Publication Date: 2015-07-01
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
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Problems solved by technology

However, the conventional anaerobic operating system separation method is not only expensive in equipment, but also has a long screening period, which hinders the screening process of dissimilatory iron-reducing bacteria

Method used

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  • Method for screening dissimilatory Fe(III)-reducing bacteria

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Experimental program
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Effect test

Embodiment 1

[0023] (1) The sediment sludge is enriched and cultivated by gradually increasing the concentration of Fe(Ⅲ) in the liquid medium: the sediment sludge used for inoculation is selected from the bottom sludge of freshwater rivers. During inoculation, add appropriate amount of mud sample to ferric citrate liquid culture medium with an initial concentration of Fe(Ⅲ) of 10mmol / L, and fill the serum bottle with nitrogen for anaerobic culture. Before anaerobic culture, fill the medium with nitrogen to the serum After the air in the bottle is completely exhausted, carry out sealed culture, after 48h of culture, determine the OD in the liquid medium 600 Value and Fe(Ⅱ) concentration, select OD 600 For liquid culture medium with value ≥0.500 and Fe(Ⅱ) concentration ≥1mmol / L, transfer the supernatant bacteria in it to ferric citrate liquid medium with Fe(Ⅲ) concentration of 20mmol / L according to 1% inoculum Medium, select OD after culturing 48h 600 The supernatant bacterial solution in the...

Embodiment 2

[0028] (1) The sediment sludge is enriched and cultivated by gradually increasing the concentration of Fe(Ⅲ) in the liquid medium: the sediment sludge used for inoculation is selected from the intertidal bottom sludge near the ocean. Except for the enrichment culture conditions, this step is exactly the same as the enrichment culture method for freshwater river sediment in step (1) of Example 1. The enrichment culture conditions in this step are: pH 8.0, temperature 35°C, and NaCl concentration It is 25g / L. After the enrichment culture, the concentration of Fe(Ⅱ) in the culture medium was measured to be 5.5 mmol / L, indicating that the bacteria in the sludge sample had obvious iron-reducing ability.

[0029] (2) Isolation and purification of dissimilar iron-reducing bacteria from the enriched flora: This step is basically the same as the method of separating and purifying dissimilar iron-reducing bacteria in step (2) of Example 1, except for the solid culture used in this step Th...

Embodiment 3

[0033] (1) The sediment sludge is enriched and cultivated by gradually increasing the concentration of Fe(Ⅲ) in the liquid culture medium: the sediment sludge used for inoculation is selected from marine sediment bottom sludge. Except for the enrichment culture conditions, this step is exactly the same as the enrichment culture method for freshwater river sediment in step (1) of Example 1. The enrichment culture conditions in this step are: pH 8.0, temperature 21°C, and NaCl concentration It is 30g / L. After the enrichment culture, the concentration of Fe(II) in the culture medium was 6mmol / L. , Which shows that the bacterial flora in the mud sample has obvious iron reduction ability.

[0034] (2) Isolation and purification of dissimilar iron-reducing bacteria from the enriched flora: This step is basically the same as the method of separating and purifying dissimilar iron-reducing bacteria in step (2) of Example 1, except for the solid culture used in this step The concentratio...

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Abstract

The invention discloses a method for screening dissimilatory Fe(III)-reducing bacteria. The method comprises the following steps: (1) carrying out enrichment culture on sediment sludge by way of gradually improving the Fe(III) concentration in a liquid culture medium; and (2) separating and purifying the dissimilatory Fe(III)-reducing bacteria from enriched flora and carrying out gradient-acclimation on heavy metal endurance capacity and salt tolerance. The method for screening dissimilatory Fe(III)-reducing bacteria is simple and feasible and short in screening period. In the screening process, expensive separating equipment such as an anaerobic operation system and the like is required, so that the screening cost is effectively lowered. The obtained pure strain has a relatively strong Fe(III) reducing capacity. In addition, through gradient-acclimation on heavy metal endurance capacity and salt tolerance of the pure strain, a dissimilatory Fe(III)-reducing bacterial strain which has the endurance capacity to Cr (VI) heavy metals and different salt concentration environments can be quickly and simply obtained, thereby providing a germplasm resource for biological repair in the environments such as a heavy metal region, a salt region and the like.

Description

Technical field [0001] The present invention relates to the technical field of environmental microorganisms, in particular to a method for screening dissimilar iron reducing bacteria. Background technique [0002] Iron is a variable-valence metal, and the biological redox of iron involves the biogeochemical cycle of multiple elements. The iron cycle process dominated by Fe(Ⅲ) and Fe(Ⅱ) is one of the key factors regulating the migration and transformation of heavy metals, and the dissimilar iron reduction process mediated by microorganisms can simultaneously achieve the oxidation and decomposition of organic pollutants under anaerobic conditions . Therefore, microorganism-mediated iron reduction process plays an important role in ecological environment and bioremediation. [0003] Due to the rapid development of modern industry and the gradual accumulation of organic pollutants in the environment, many polluted environments provide an anaerobic or hypoxic condition. In an anaerob...

Claims

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Application Information

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IPC IPC(8): C12N1/20
CPCC12N1/20C12N1/36
Inventor 刘洪艳王红玉马帅殷艳艳
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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