Application of anti-NMDAR autoantibody as congenital megacolon diagnostic marker

A technology for Hirschsprung's disease and autoantibodies, applied in the field of biomedicine, can solve the problems of inconspicuous barium enema, influential results and high price, and achieve the effects of good sensitivity and specificity, simple operation and low cost

Active Publication Date: 2021-07-09
GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rectal biopsy is to directly take rectal tissue to detect whether there is a loss of ganglion cells. The accuracy rate is high, but the sampling site will affect the result
This method is interventional and invasive, and the price is very high. Generally, this method is applied to children whose barium enema is not obvious or not applicable, such as when the child has the possibility of necrotizing enterocolitis (NEC) , b

Method used

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  • Application of anti-NMDAR autoantibody as congenital megacolon diagnostic marker
  • Application of anti-NMDAR autoantibody as congenital megacolon diagnostic marker
  • Application of anti-NMDAR autoantibody as congenital megacolon diagnostic marker

Examples

Experimental program
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Example Embodiment

[0079] Example 1. Plasma and tissue sample collection and grouping

[0080] Plasma samples were divided into Hirschsprung's disease children group (37 cases), other enteropathy control group (18 cases), healthy children group (30 cases), the age ranged from 3 months to 3 years old, and the gender was 3 / 4 male. Male, disease and control groups were matched for age and sex. All samples were obtained from Guangzhou Women's and Children's Medical Center, and the healthy children group was the remaining blood samples after physical examination of healthy children. The blood collection method is anticoagulation blood collection, centrifugation to separate plasma, and frozen samples. Colon tissue samples were from the group of children with Hirschsprung's disease (36 cases), which were surgically resected lesions. Other enteropathy control group (including 11 cases of colon tissue with anal stricture and intestinal stricture fistula).

Example Embodiment

[0081] Example 2. Human autoimmune antigen chip screening and differentially expressed autoantibody analysis

[0082] Plasma from 5 children with Hirschsprung's disease (HSCR), 5 from healthy group (HC) and 5 from other enteropathy (DC) groups were used for the screening of human neuroimmune chip, which was developed by Guangzhou Yijin Biotechnology Co., Ltd. Provided by Technology Co., Ltd., including the IgG detection of more than 100 kinds of neural autoimmune antibodies, the original data, after subtracting the negative control, were normalized by the RLM method, and the results were analyzed by M statistics. figure 1 Cluster analysis graph showing differential autoantibodies, from which it can be seen that anti-N-methyl-D-aspartate receptor (NMDAR) autoantibodies were significantly higher in plasma from children with Hirschsprung's disease than in controls ( p=0.018).

Example Embodiment

[0083] Example 3. Detection of anti-N-methyl-D-aspartate receptor (NMDAR) autoantibodies in Hirschsprung's disease tissue

[0084] N-methyl-D-aspartate receptor (NMDAR) is a protein specifically expressed in neural tissue, and autoantibodies to this protein have been reported in encephalitis, but not in peripheral enteric nervous system diseases. In order to verify that the anti-N-methyl-D-aspartate receptor (NMDAR) autoantibodies are indeed derived from the enteric nerve tissue of the diseased segment of megacolon patients, the intestinal tissues of the diseased segment of megacolon patients were collected (36 cases), and Colon tissue of other intestinal diseases (including 11 cases of colon tissue with anal stenosis and intestinal stenosis), the anti-N-methyl-D-aspartate receptor (NMDAR) in the tissue was detected by enzyme-linked immunosorbent assay (ELISA). The level of autoantibodies was detected by a human anti-N-methyl-D-aspartate receptor (NMDAR) autoantibody antibody ...

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Abstract

The invention relates to the technical field of biological medicine, and in particular, relates to an application of an anti-NMDAR autoantibody as a congenital megacolon diagnostic marker. It is found that the anti-NMDAR autoantibody has the advantages of being easy to operate, free of intervention, high in flux and low in cost in children congenital disease diagnosis, and the defect that plasma diagnosis is not available in the prior art is overcome. The method has good diagnosis sensitivity and specificity, the AUC value is 0.9046, the optimal limit corresponding sensitivity is 93.75%, and the specificity is 81.58%. Besides, children suffering from the congenital megacolon are represented as vomiting, abdominal distension, constipation, enteritis and the like, the children suffering from the symptoms are large, the children suffering from the congenital megacolon account for a small amount, and a simple and effective screening method is needed. When the method is used for disease screening, the sensitivity is 100%, the specificity is 78.95, and the method can be used for disease screening.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of an anti-NMDAR autoantibody as a diagnostic marker for Hirschsprung's disease. Background technique [0002] Hirschsprung disease (HSCR) is a birth defect disease with abnormal development of enteric nerves in children. The pathological mechanism is that the migration and differentiation of enteric neural crest cells into enteric neurons are impaired, resulting in the absence of enteric nerves and persistent spasm. It is one of the common congenital intestinal diseases in children. The early manifestations of Hirschsprung's disease are vomiting, abdominal distension, diarrhea, etc. Clinically, it will cause neonatal death or complications such as recurrent enteritis after surgery, refractory constipation, etc., seriously affecting the growth and quality of life of the children. [0003] Timely diagnosis and treatment of Hirschsprung can reduce the risk of H...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/065
Inventor 朱云张彦
Owner GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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