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Application of anti-NMDAR autoantibody as congenital megacolon diagnostic marker

A technology for Hirschsprung's disease and autoantibodies, applied in the field of biomedicine, can solve the problems of inconspicuous barium enema, influential results and high price, and achieve the effects of good sensitivity and specificity, simple operation and low cost

Active Publication Date: 2021-07-09
GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rectal biopsy is to directly take rectal tissue to detect whether there is a loss of ganglion cells. The accuracy rate is high, but the sampling site will affect the result
This method is interventional and invasive, and the price is very high. Generally, this method is applied to children whose barium enema is not obvious or not applicable, such as when the child has the possibility of necrotizing enterocolitis (NEC) , barium enema can lead to intestinal perforation, at this time it is not suitable to use barium enema for diagnosis, only consider rectal biopsy
Rectal manometry is to judge the abnormal innervation of intestinal nerve by detecting the lack of relaxation of the internal anal sphincter. It is only an auxiliary diagnostic method, and there are many false positives and false negatives. It cannot be used for independent detection

Method used

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  • Application of anti-NMDAR autoantibody as congenital megacolon diagnostic marker
  • Application of anti-NMDAR autoantibody as congenital megacolon diagnostic marker
  • Application of anti-NMDAR autoantibody as congenital megacolon diagnostic marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1. Collection and Grouping of Plasma and Tissue Samples

[0080] Plasma samples were divided into Hirschsprung children group (37 cases), other enteropathy control group (18 cases), healthy children group (30 cases), aged from 3 months to 3 years old, male sex 3 / 4 Male, disease and control groups were age and sex matched. All samples came from Guangzhou Women and Children's Medical Center, and the healthy children group was the remaining blood samples after physical examination of healthy children. The blood collection method is anticoagulant blood collection, centrifugation to separate plasma, and cryopreservation of samples. The colon tissue samples belonged to the Hirschsprung children group (36 cases), which were surgically resected diseased tissues. Other enteropathy control group (including 11 cases of colon tissue with anal stenosis and intestinal stricture stoma).

Embodiment 2

[0081] Example 2. Human autoimmune antigen chip screening and differentially expressed autoantibody analysis

[0082] The plasma of 5 cases of children with Hirschsprung's disease (HSCR), 5 cases of plasma from children with healthy group (HC) and 5 cases of children with other intestinal diseases (DC) were used for the screening of human neuroimmune chips. Provided by Technology Co., Ltd., including more than 100 kinds of IgG detection of neural autoimmune antibodies, the original data, after subtracting the negative control, were normalized by the RLM method, and the results were analyzed by M statistics. figure 1 The cluster analysis diagram of the differential autoantibodies is shown, from which it can be seen that the anti-N-methyl-D-aspartate receptor (NMDAR) autoantibodies were significantly higher in the plasma of children with Hirschsprung than in the control group ( p=0.018).

Embodiment 3

[0083] Example 3. Detection of autoantibodies against N-methyl-D-aspartate receptor (NMDAR) in Hirschsprung disease tissue

[0084] N-methyl-D-aspartate receptor (NMDAR) is a protein expressed specifically in nerve tissue. Autoantibodies to this protein have been reported in encephalitis, but not in peripheral enteric nervous system diseases. In order to verify that the anti-N-methyl-D-aspartate receptor (NMDAR) autoantibody is indeed derived from the enteric nerve tissue of the lesion of the patient with Hirschsprung, the intestinal tissue of the lesion of the patient with Hirschsprung was collected (36 cases), and Colonic tissues of other intestinal diseases (including 11 cases of colonic tissues of anal stricture and enterostomy) were detected by enzyme-linked immunosorbent immunoassay (ELISA) for anti-N-methyl-D-aspartate receptor (NMDAR) For the level of autoantibodies, the detection kit is human anti-N-methyl-D-aspartate receptor (NMDAR) autoantibody antibody enzyme-link...

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Abstract

The invention relates to the technical field of biological medicine, and in particular, relates to an application of an anti-NMDAR autoantibody as a congenital megacolon diagnostic marker. It is found that the anti-NMDAR autoantibody has the advantages of being easy to operate, free of intervention, high in flux and low in cost in children congenital disease diagnosis, and the defect that plasma diagnosis is not available in the prior art is overcome. The method has good diagnosis sensitivity and specificity, the AUC value is 0.9046, the optimal limit corresponding sensitivity is 93.75%, and the specificity is 81.58%. Besides, children suffering from the congenital megacolon are represented as vomiting, abdominal distension, constipation, enteritis and the like, the children suffering from the symptoms are large, the children suffering from the congenital megacolon account for a small amount, and a simple and effective screening method is needed. When the method is used for disease screening, the sensitivity is 100%, the specificity is 78.95, and the method can be used for disease screening.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of an anti-NMDAR autoantibody as a diagnostic marker for Hirschsprung's disease. Background technique [0002] Hirschsprung disease (HSCR) is a birth defect disease with abnormal development of enteric nerves in children. The pathological mechanism is that the migration and differentiation of enteric neural crest cells into enteric neurons are impaired, resulting in the absence of enteric nerves and persistent spasm. It is one of the common congenital intestinal diseases in children. The early manifestations of Hirschsprung's disease are vomiting, abdominal distension, diarrhea, etc. Clinically, it will cause neonatal death or complications such as recurrent enteritis after surgery, refractory constipation, etc., seriously affecting the growth and quality of life of the children. [0003] Timely diagnosis and treatment of Hirschsprung can reduce the risk of H...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/065
Inventor 朱云张彦
Owner GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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