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Preparation and use method of microbial immobilized material for remedying polluted soil

A technology for immobilizing microorganisms and polluting soil, which is applied in the field of preparation of immobilized microorganisms, can solve the problems of large-scale application of microbial remediation and low concentration of effective degradation bacteria, and achieve high immobilization efficiency, improved resistance, and environmental protection. friendly effect

Inactive Publication Date: 2009-02-25
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low concentration of effective degrading bacteria per unit volume, microbial remediation cannot be applied to in-situ contaminated soil remediation on a large scale, and microbial immobilization technology can overcome these disadvantages

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1) Crushing or crushing the waste soybean stalk raw material after air drying into 1-20mm particles;

[0018] 2) carbonize the biomass particles at 150° C. and sterilize them under oxygen-limited conditions for 0.5 hours to obtain a carrier material for immobilized microorganisms;

[0019] 3) After inserting the white rot fungus suspension at a solid-to-liquid ratio of 0.5mL / g, carry out proliferation and culture for 12 hours, and then obtain the microorganism-immobilized material;

[0020] 4) Add the prepared microbial immobilization material to PAH-contaminated soil at a rate of 200 tons per hectare, and plow the surface 0-30cm of soil to fully mix it with the microbial immobilization material to pollute the soil Simultaneous bioremediation and production of safe agricultural products.

Embodiment 2

[0022] 1) Crushing or crushing the waste soybean stalk raw material after air drying into 1-20mm particles;

[0023] 2) Carbonize and sterilize the biomass particles at 300°C and oxygen-limited conditions for 1 hour to prepare the carrier material for immobilized microorganisms

[0024] 3) After inserting the white-rot fungal suspension at a solid-to-liquid ratio of 2 mL / g, carry out proliferation and culture for 24 hours, and then obtain the microorganism immobilized material;

[0025] 4) Add the prepared microbial immobilization material to PAH-contaminated soil at a rate of 10 tons per hectare, and plow the surface 0-30cm of soil to fully mix it with the microbial immobilization material to pollute the soil Simultaneous bioremediation and production of safe agricultural products.

Embodiment 3

[0027] 1) Crushing or crushing the waste soybean stalk raw material after air drying into 1-20mm particles;

[0028] 2) Carbonize and sterilize the biomass particles at 400°C and oxygen-limited conditions for 5 hours to prepare the carrier material for immobilized microorganisms

[0029] 3) After inserting the white-rot fungal suspension at a solid-to-liquid ratio of 1 mL / g, carry out proliferation and culture for 12 hours, and then obtain the microorganism immobilized material;

[0030] 4) Add the prepared microbial immobilization material to the PAH-contaminated soil at a rate of 100 tons per hectare, and plow the surface 0-30cm of soil to fully mix it with the microbial immobilization material to pollute the soil Simultaneous bioremediation and production of safe agricultural products.

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Abstract

The invention discloses a method for preparing and using a microbial immobilized material for repairing contaminated soil. The method comprises the following steps: (1) after being dried by air, the waste biomass raw material is pulverized or ground into 1-20mm of particles; (2) the biomass particles are carbonized and sterilized at the temperature of 150-450 DEG C and under limited oxygen condition for 0.5-6 hours to prepare a carrier material; (3) the carrier material is put into bacterium suspension for enrichment culture according to the solid-to-liquid ratio of 0.5-2mL / g, then the microbial immobilized material is produced; (4) the produced microbial immobilized material is added to organic contaminated soil according to the proportion of 1-500 tons per hectare soil, 0-30cm of topsoil is ploughed to cause the microbial immobilized material to be mixed evenly in the soil, simultaneously, the contaminated soil is biologically repaired and safe agricultural products are produced. The method has the advantages that the carrier material contains abundant lignin structures, carbon and nutrient elements, which provides more suitable matrix for microbial growth; and the immobilized material has strong affinity, high immobilization efficiency, strong adsorption capacity and good resistance against the pollutants in the environment; and the immobilized material is a soil fertilizer, and is suitable for large-scale in-situ biological repair of the contaminated soil.

Description

technical field [0001] The invention relates to a microorganism immobilization technology, in particular to a method for preparing and using a microorganism immobilization material for remediation of contaminated soil. Background technique [0002] Soil is an important part of the human environment, an indispensable and non-renewable natural resource, and an important basis for the survival of all living things and the growth of crops. At the same time, soil is the source and sink of various pollutants. With the development of modern industrial and agricultural production, large-scale application of chemical fertilizers and pesticides, industrial waste water continuously invades farmland, urban industrial waste and other artificial synthetic substances continuously enter the soil, landfill leakage, oil well mining and atmospheric subsidence cause soil environmental pollution accidents Keep happening. After pollutants enter the soil, they not only affect the growth and deve...

Claims

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Application Information

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IPC IPC(8): C12N11/02B09C1/10
Inventor 陈宝梁王银善元妙新
Owner ZHEJIANG UNIV
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