18 results about "Retinal tissue" patented technology

This invention discloses a novel structural polysaccharide SYQE3-F derived from the roots of *Tripterygium wilfordii*, its preparation method, pharmaceutical composition, and applications, belonging to the field of biomedical technology. This invention is the first to isolate and purify a homogeneous polysaccharide SYQE3-F from the roots of *Tripterygium wilfordii*, with an average molecular weight of 13.174 kDa. Its main chain consists of →4)-β-D-GlcpA-(1→) and →2)-α-D-Manp-(1→), and its side chains include t-Galp and t-Araf. This invention also discloses a pharmaceutical composition containing this polysaccharide and vitamin E. In vitro and in vivo experiments show that this polysaccharide can effectively break down choroidal occlusion barriers and significantly promote the transport of vitamin E to the target retinal tissue, providing a novel natural drug combination for the clinical treatment of DR.

Optical coherence tomography (OCT) data can be analyzed with neural networks trained on OCT data and known clinical outcomes to make more accurate predictions about the development and progression of retinal diseases, central nervous system disorders, and other conditions. The methods take 2D or 3D OCT data derived from different light source configurations and analyze it with neural networks that are trained on OCT images correlated with known clinical outcomes to identify intensity distributions or patterns indicative of different retina conditions. The methods have greater predictive power than traditional OCT analysis because the invention recognizes that subclinical physical changes affect how light interacts with the tissue matter of the retina, and these intensity changes in the image can be distinguishable by a neural network that has been trained on imaging data of retinas.

Provided herein are methods and systems for determining a parameter across a surface of a tissue such as a retinal tissue of an eye of a subject. In some cases, the determined parameter is tissue oxygenation which can be used for a variety of clinical applications such as diagnosing and / or modeling a disease in the subject. In some examples, the method is performed by detecting one or more optical signals from the tissue using an optical detector, and processing the detected optical signals to characterize and map the parameter across the surface of the tissue. The methods and systems provided herein can prove accurate physics-based models of parameters such as tissue oxygenation across a heterogeneous tissue surface.

A method for preventing the retinal pigment from being removed from the network, comprising the steps of pre-fixing liquid fixation, solidifying agent wrapping shaping, fixing liquid fixation, embedding agent embedding, melanin decomposition liquid decomposing melanin, pre-decoloring liquid pre-decoloring, decoloring liquid decoloring and rinsing liquid elution. A method for removing melanin is provided to effectively avoid the retinal RPE layer cell from being removed from the network, protein denaturation and nucleic acid degradation. The method mainly adopts the following steps: pre-fixing liquid to maintain the original state of tissue cells, solidifying agent to maintain the integrity of tissue structure, melanin decomposition liquid composed of various extracts to decompose melanin particles, pre-decoloring liquid and decoloring liquid to separate melanin, and rinsing liquid to elute the decomposed melanin on the surface of the tissue, etc. Under the premise of ensuring the integrity of the retinal tissue structure, not damaging the surface protein, nucleic acid and cell structure of the tissue section, effectively decomposing the melanin particles in the tissue section, maintaining the biological activity of various organelles on the section surface and meeting the needs of subsequent various histochemical staining.

The present invention provides a method for maintaining a continuous epithelial structure of a retinal tissue including culturing the retinal tissue in a medium comprising a methyl group donor or a substrate of the methyl group donor at a concentration at which cell differentiation of a neural retinal progenitor cell is suppressed, and a neurite extension inhibitor at a concentration at which neurite extension is suppressed.

A method and apparatus for identifying fundus lesions are provided. The method includes: acquiring OCT fundus images of a specific patient; using a first neural network model to determine the boundary information of retinal tissue in the OCT fundus images, and segmenting the OCT fundus images into multiple image blocks based on the boundary information of the retinal tissue; providing the multiple image blocks to a second neural network model to obtain the fundus lesion type of the specific patient; the second neural network model calculating the feature vector of each image block and the attention weight of each image block for the specified fundus lesion type, and obtaining the fused feature vector through weighted calculation to obtain the identification result for the specified fundus lesion type. The second neural network model combines spatial saliency and scanning position saliency to enhance important information in the image block group and filter out unimportant information, thereby achieving low-cost model training and application.

A tissue transplantation resin tip and the like that allow easy transplantation of a tissue sucked inside are provided. A tissue transplantation resin tip 10 according to the present invention includes a needle tube 1 that can be inserted into an eyeball. The needle tube includes a main body portion 11 that extends linearly, and a front end portion 12 that is bent with respect to the main body portion and extends linearly, and that discharges a tissue T from a front end 121. A length L1 of the needle tube is 25 mm or more and 50 mm or less, a cross-sectional external shape of the front end portion has an oval shape having a major axis D1 of 0.8 mm or more and 1.5 mm or less, and a minor axis D2 of 0.5 mm or more and 1.0 mm or less, the front end of the front end portion is a bevel surface that extends in a direction substantially perpendicular to the main body portion, and that has a bevel angle Θ1 of 50° or more and 85° or less, the hardness of the front end portion is 0.15 N or more and 0.30 N or less, and the hardness of the center of the needle tube is 0.80 N or more and 1.50 N or less.

The utility model provides a kind of special stage of retinal low temperature stripping and experimental equipment, it is related to experimental apparatus field. Including stage body, the top of stage body is horizontal plane, and recess is set in its top;Ice water channel is set below recess, and ice water channel is through stage body. By setting the stage body of top surface level, and setting recess to place retina tissue, so that the stripping operation of retina can be stably carried out, and tissue is not easy to shift;Ice water channel is set for the flow of ice water mixture, keep the low temperature of stage body, since it is just below recess, and then cold quantity can be transmitted to recess faster, ice water mixture is directly replenished to keep low temperature continuously, and PBS solution is not needed to be pre-cooled frequently, so that liquid exchange is convenient and fast;Stage body can also support operator's both hands, improve operation stability.

The utility model provides an ophthalmic surgical instrument for posterior detachment of a vitreous body, and relates to the technical field of medical instruments. The ophthalmic surgical instrument comprises an injection needle cylinder and a liquid supply pipe connected with the outlet end of the injection needle cylinder, and the end, away from the injection needle cylinder, of the liquid supply pipe is communicated with an injection needle with a silica gel soft head; a slidable piston is arranged in the injection needle cylinder, and the piston rod is detachably connected with the piston. The device is simple in structure, can be manufactured by existing medical common instruments, and can be reused, so that the cost of ophthalmologic operations is reduced; the silicone oil injection device can be connected with a silicone oil injection pipeline of the vitreous cutter for flow control and automatic injection, is stable in operation, saves manpower, and can reduce disturbance to retina tissues caused by unbalanced manual injection liquid flow.

A tissue transplantation resin tip includes a needle tube for eyeball insertion. The needle tube includes a main body portion that extends linearly. A front end portion is bent with respect to the main body portion and extends linearly, with tissue discharging from the front end. The needle tube length is 25 mm to 50 mm, the front end portion cross sectional shape is oval with a major axis D1 of 0.8 mm to 1.5 mm and a minor axis D2 of 0.5 mm to 1.0 mm. The front end of the front end portion is a bevel surface that extends in a direction substantially perpendicular to the main body portion and has a bevel angle of 50° to 85°. The front end portion hardness is 0.15 N to 0.30 N or less and the center of the needle tube hardness is 0.80 N to 1.50 N.

The invention discloses an application of a body fluid extracellular vesicle membrane protein in preparation of a product for diagnosing glaucoma disease, the outer vesicle membrane protein is selected from Tau protein and / or GFAP protein on outer vesicles, the invention firstly proposes that NDEVs in peripheral blood are captured through specific membrane proteins (GLAST and L1CAM); therefore, pathological changes of the central nervous system and the retina tissue can be reflected more accurately.

Methods and systems for measuring hemoglobin concentration (cHb). A retinal tissue having blood vessels is illuminated by light including two isosbestic wavelengths (λ1,λ2). The isosbestic wavelengths(λ1,λ2) correspond to isosbestic points where oxy- and deoxyhemoglobin (HbIO2,Hb) have the same molar extinction coefficient. Measurements are collected of a plurality of backscattered reflection intensities (I(χb,λ1), I(xt,λ1), I(χb,λ2), I(xx,X2)). Preferably, the measurements are spectrally resolved at least for the two isosbestic wavelengths (λ1,λ2) and position-resolved for at least a blood vessel location (xb) coinciding with a selected blood vessel and a tissue location (xt) coinciding with the retinal tissue without blood vessel. The hemoglobin concentration (cHb) can be calculated based on a combination of the measurements at the two isosbestic wavelengths (λt,λ2).

Photovoltaic retinal prosthesis is provided with optically configurable confinement of electrical field. A video stream is projected onto a retinal implant. An array of photovoltaic pixels is configured to provide retinal stimulus responsive to the video stream. The photovoltaic pixels have a common return electrode. Each pixel has an active electrode that is coupled to retinal tissue via a capacitive interface or a faradaic interface. Each pixel includes photodiode(s) connected in series between the common return electrode and the corresponding active electrode. The projected video stream is configured based on a source video stream such that one or more pixels of the retinal implant that will be dark in a next frame of the projected video stream are optically preconditioned (pre-charged) by the projected video stream during the previous frame to become sufficiently conductive to act as transient local return electrodes during the next frame of the projected video stream.

The present disclosure provides methods and an apparatus for imaging and analysing images of presumed protein deposits in the retina, retinal tissue or retinal structures and discloses methods differentiating or classifying these deposits and other optical signals from retinal structures into 1) whether they contain or do not contain classes, of proteins or protein deposits called amyloids or other proteins and / or protein deposits related to neurodegenerative eye and brain disease(s); 2) which type(s) of amyloid or other proteins or protein deposits they contain, as well as 3) whether the form and / or properties of the deposit are associated with a class of diseases or with one or another specific condition(s) (or disease(s)); whether or not this is a disease or class of disease associated with the retina or more generally with the nervous system, including the brain or 4) classified as associated with one or another level of severity of condition(s), or disease(s).

An embodiment relates to a safety vitreous body cutting device capable of safely removing only a vitreous body attached to a retina without damaging the retina. Here, the safety vitreous body cutting device includes a tubular body part, a cutter part, and a safety net part. The tubular body part is formed in a cylindrical shape, negative pressure is provided at the inside, and an open hole is formed on the outer circumferential surface of a distal end. The cutter part reciprocates along the axial direction of the tubular body part at the inside of the tubular body part to cut the vitreous body introduced into the open hole. The safety net part is provided at the distal end of the tubular body part, and allows the vitreous body to pass through, but prevents the retina tissue from passing through.

The invention provides a method for producing a retinal tissue by (1) subjecting pluripotent stem cells to floating culture in a serum-free medium containing a substance inhibiting the Wnt signal pathway to form an aggregate of pluripotent stem cells, (2) subjecting the aggregate to floating culture in a serum-free medium containing a basement membrane preparation, and then (3) subjecting the aggregate to floating culture in a serumcontaining medium. The invention also provides a method for producing an optic-cup-like structure, a method for producing a retinal pigment epithelium, and a method for producing a retinal layer-specific neural cell.

The general inventive concepts contemplate methods and compositions for modifying certain cell lines to facilitate the production of retinal tissue from human induced pluripotent stem cells. Human induced pluripotent stem cells can be cultured to develop into retinal organoids, among other cells types. The retinal organoids play a role in modeling both human retinal development and retinal disease. The methods and compositions discussed herein provide means to measure and monitor human induced pluripotent stem cells during development.

Provided herein are optically transparent materials configured to block an appropriate amount of incident blue light, such that when the materials are positioned in the optical path between environmental light and the retina of a user, the optically transparent materials reduce the amount of blue light from the environmental light that reaches the retina of a user. The materials can block an effective amount of blue light to minimize damage to retinal tissue while permitting transmission of an effective amount of maintain acceptable photopic vision, scotopic vision, color vision, and circadian rhythms.