41 results about "Retinal tissue" patented technology

The present invention provides a method for producing a retinal progenitor cell, including (1) a first step of subjecting pluripotent stem cells to floating culture in a serum-free medium to form an aggregate of pluripotent stem cells, and (2) a second step of subjecting the aggregate formed in step (1) to floating culture in a serum-free medium or serum-containing medium each being free of a substance acting on the Sonic hedgehog signal transduction pathway but containing a substance acting on the BMP signal transduction pathway, thereby obtaining an aggregate containing retinal progenitor cells.

The present invention provides a method for producing retinal cells or a retinal tissue, comprising the following steps (1)-(3):(1) a first step of culturing human pluripotent stem cells in the absence of feeder cells and in a medium comprising a factor for maintaining undifferentiated state,(2) a second step of culturing the pluripotent stem cells obtained in the first step in suspension in the presence of a Sonic hedgehog signal transduction pathway activating substance to form a cell aggregate, and(3) a third step of culturing the aggregate obtained in the second step in suspension in the presence of a 1) a BMP signal transduction pathway activating substance to obtain an aggregate containing retinal cells or a retinal tissue.

Phase-based optoretinography (ORG) is performed using tissue velocity obtained from a modified optical coherence tomography (OCT) system. A swept-source generated OCT A-scan is used to assemble a 2-dimensional profile of the retinal tissue (B-scan) by laterally scanning the imaging beam. These B-scans can be taken at multiple time-points and assembled into a M-scan, which displays motion of a particular cross-section of retinal tissue (after correction for bulk motion). The method captures the changes between each timestep of the M-scan to deduce tissue velocities of the COST, ROST, and IS / OS, among others. An initial contraction of outer segments is detected in outer segments, followed by an elongation shortly thereafter. The tissue velocity measurements can be used to infer retinal dysfunction without the need for costly and computationally expensive scans using adaptive optics.

This invention discloses a novel structural polysaccharide SYQE3-F derived from the roots of *Tripterygium wilfordii*, its preparation method, pharmaceutical composition, and applications, belonging to the field of biomedical technology. This invention is the first to isolate and purify a homogeneous polysaccharide SYQE3-F from the roots of *Tripterygium wilfordii*, with an average molecular weight of 13.174 kDa. Its main chain consists of →4)-β-D-GlcpA-(1→) and →2)-α-D-Manp-(1→), and its side chains include t-Galp and t-Araf. This invention also discloses a pharmaceutical composition containing this polysaccharide and vitamin E. In vitro and in vivo experiments show that this polysaccharide can effectively break down choroidal occlusion barriers and significantly promote the transport of vitamin E to the target retinal tissue, providing a novel natural drug combination for the clinical treatment of DR.

In some embodiments, a system for providing a therapeutic treatment to a patient's eye includes a treatment beam source configured to transmit a treatment beam along a treatment beam path. The system further includes a processor coupled to the treatment beam source, the processor being configured to direct the treatment beam onto retinal tissue of the patient's eye and deliver a series of short duration pulses from the treatment beam onto the retinal tissue at a first treatment spot to treat the retinal tissue. In some embodiments, a pre-treatment evaluation method using electroretinography (ERG) data may be used to predict effects of treatment beams at different power values and to determine optimal power values.

The present invention relates to the application of an EphA2-specific tyrosine kinase inhibitor, ALW-II-41-27, in ophthalmic diseases. The present invention has confirmed that at a specific concentration, ALW-II-41-27 can inhibit the expression of EphA2 in the retina, reduce the damage of acute high intraocular pressure to retinal tissue, and improve the survival rate of retinal ganglion cells, thereby playing a neuroprotective role, and thus being used for the preparation of treatments for preventing and treating acute high intraocular pressure or glaucoma.

Optical coherence tomography (OCT) data can be analyzed with neural networks trained on OCT data and known clinical outcomes to make more accurate predictions about the development and progression of retinal diseases, central nervous system disorders, and other conditions. The methods take 2D or 3D OCT data derived from different light source configurations and analyze it with neural networks that are trained on OCT images correlated with known clinical outcomes to identify intensity distributions or patterns indicative of different retina conditions. The methods have greater predictive power than traditional OCT analysis because the invention recognizes that subclinical physical changes affect how light interacts with the tissue matter of the retina, and these intensity changes in the image can be distinguishable by a neural network that has been trained on imaging data of retinas.

The present invention provides a method for producing a retinal progenitor cell, including (1) a first step of subjecting pluripotent stem cells to floating culture in a serum-free medium to form an aggregate of pluripotent stem cells, and (2) a second step of subjecting the aggregate formed in step (1) to floating culture in a serum-free medium or serum-containing medium each being free of a substance acting on the Sonic hedgehog signal transduction pathway but containing a substance acting on the BMP signal transduction pathway, thereby obtaining an aggregate containing retinal progenitor cells.

Provided herein are methods and systems for determining a parameter across a surface of a tissue such as a retinal tissue of an eye of a subject. In some cases, the determined parameter is tissue oxygenation which can be used for a variety of clinical applications such as diagnosing and / or modeling a disease in the subject. In some examples, the method is performed by detecting one or more optical signals from the tissue using an optical detector, and processing the detected optical signals to characterize and map the parameter across the surface of the tissue. The methods and systems provided herein can prove accurate physics-based models of parameters such as tissue oxygenation across a heterogeneous tissue surface.

The present disclosure provides methods and an apparatus for imaging and analysing images of presumed protein deposits in the retina, retinal tissue or retinal structures and discloses methods differentiating or classifying these deposits and other optical signals from retinal structures into 1) whether they contain or do not contain classes, of proteins or protein deposits called amyloids or other proteins and / or protein deposits related to neurodegenerative eye and brain disease(s): 2) which type(s) of amyloid or other proteins or protein deposits they contain, as well as 3) whether the form and / or properties of the deposit are associated with a class of diseases or with one or another specific condition(s) (or disease(s)); whether or not this is a disease or class of disease associated with the retina or more generally with the nervous system, including the brain or 4) classified as associated with one or another level of severity of condition(s), or disease(s).

A method for preventing the retinal pigment from being removed from the network, comprising the steps of pre-fixing liquid fixation, solidifying agent wrapping shaping, fixing liquid fixation, embedding agent embedding, melanin decomposition liquid decomposing melanin, pre-decoloring liquid pre-decoloring, decoloring liquid decoloring and rinsing liquid elution. A method for removing melanin is provided to effectively avoid the retinal RPE layer cell from being removed from the network, protein denaturation and nucleic acid degradation. The method mainly adopts the following steps: pre-fixing liquid to maintain the original state of tissue cells, solidifying agent to maintain the integrity of tissue structure, melanin decomposition liquid composed of various extracts to decompose melanin particles, pre-decoloring liquid and decoloring liquid to separate melanin, and rinsing liquid to elute the decomposed melanin on the surface of the tissue, etc. Under the premise of ensuring the integrity of the retinal tissue structure, not damaging the surface protein, nucleic acid and cell structure of the tissue section, effectively decomposing the melanin particles in the tissue section, maintaining the biological activity of various organelles on the section surface and meeting the needs of subsequent various histochemical staining.

The present invention provides a method for maintaining a continuous epithelial structure of a retinal tissue including culturing the retinal tissue in a medium comprising a methyl group donor or a substrate of the methyl group donor at a concentration at which cell differentiation of a neural retinal progenitor cell is suppressed, and a neurite extension inhibitor at a concentration at which neurite extension is suppressed.

A method and apparatus for identifying fundus lesions are provided. The method includes: acquiring OCT fundus images of a specific patient; using a first neural network model to determine the boundary information of retinal tissue in the OCT fundus images, and segmenting the OCT fundus images into multiple image blocks based on the boundary information of the retinal tissue; providing the multiple image blocks to a second neural network model to obtain the fundus lesion type of the specific patient; the second neural network model calculating the feature vector of each image block and the attention weight of each image block for the specified fundus lesion type, and obtaining the fused feature vector through weighted calculation to obtain the identification result for the specified fundus lesion type. The second neural network model combines spatial saliency and scanning position saliency to enhance important information in the image block group and filter out unimportant information, thereby achieving low-cost model training and application.

Provided is a method for quickly, objectively and properly evaluating the quality of a cell aggregate by determining the quality of the cell aggregate by determining an evaluation score on the basis of a determination criterion from an appropriate point of view with respect to an image of the cell aggregate.　With respect to a cell aggregate which can include a retinal tissue derived from a pluripotent stem cell, in an image region of the cell aggregate which is each of an inverted observation image of the cell aggregate and an elected observation image of the cell aggregate, a region that is determined as satisfying a color-related candidate region determination criterion for each of the inverted observation image and the elected observation image on the basis of the candidate region determination criterion is identified as a candidate region included in a region that is a candidate for a normal retinal tissue region. Subsequently, in the candidate region of each of the inverted observation image and the elected observation image, a candidate region that is determined as satisfying a brightness- and / or shape-related normal region determination criterion for each of the inverted observation image and the elected observation image is determined as the normal retinal tissue region.

The present disclosure includes: a method for producing a retinal tissue, the method comprising: (1) inoculating pluripotent stem cells to an area A having cell adhesion on a culture substrate at a density of 0.5 * 105 cells / cm < 2 > to 2.5 * 105 cells / cm < 2 >, (2) culturing the pluripotent stem cells inoculated in step (1) in a culture medium containing a ROCK inhibitor for 1-16 hours, and (3) culturing the pluripotent stem cells inoculated in step (1) in a culture medium containing a ROCK inhibitor for 1-16 hours, and (3) culturing the cells obtained after step (2) in a culture medium comprising a BMP signaling activator; a retinal tissue produced by the method; a composition comprising the retinal tissue; and the like.

A tissue transplantation resin tip and the like that allow easy transplantation of a tissue sucked inside are provided. A tissue transplantation resin tip 10 according to the present invention includes a needle tube 1 that can be inserted into an eyeball. The needle tube includes a main body portion 11 that extends linearly, and a front end portion 12 that is bent with respect to the main body portion and extends linearly, and that discharges a tissue T from a front end 121. A length L1 of the needle tube is 25 mm or more and 50 mm or less, a cross-sectional external shape of the front end portion has an oval shape having a major axis D1 of 0.8 mm or more and 1.5 mm or less, and a minor axis D2 of 0.5 mm or more and 1.0 mm or less, the front end of the front end portion is a bevel surface that extends in a direction substantially perpendicular to the main body portion, and that has a bevel angle Θ1 of 50° or more and 85° or less, the hardness of the front end portion is 0.15 N or more and 0.30 N or less, and the hardness of the center of the needle tube is 0.80 N or more and 1.50 N or less.

The invention discloses a retinal organoid culture medium and application thereof, and relates to the field of organoid culture. The culture medium comprises a basic culture medium and specific addition factors, wherein the specific addition factors comprise A8301, Forskolin, Prostaglandin E2, R-Spondin protein, Noggin protein and FGF10 (Fibroblast Growth Factor 10) protein, and the specific addition factors comprise A8301, Forskolin, Prostaglandin E2, R-Spondin protein, Noggin protein and FGF10 (Fibroblast Growth Factor 10) protein. The culture medium disclosed by the invention can be used for obtaining retina organoid culture from retina tissue culture, and is high in organoid formation rate, high in biological consistency, high in research value and wide in application prospect.

The utility model provides a kind of special stage of retinal low temperature stripping and experimental equipment, it is related to experimental apparatus field. Including stage body, the top of stage body is horizontal plane, and recess is set in its top;Ice water channel is set below recess, and ice water channel is through stage body. By setting the stage body of top surface level, and setting recess to place retina tissue, so that the stripping operation of retina can be stably carried out, and tissue is not easy to shift;Ice water channel is set for the flow of ice water mixture, keep the low temperature of stage body, since it is just below recess, and then cold quantity can be transmitted to recess faster, ice water mixture is directly replenished to keep low temperature continuously, and PBS solution is not needed to be pre-cooled frequently, so that liquid exchange is convenient and fast;Stage body can also support operator's both hands, improve operation stability.

Optical coherence tomography (OCT) data can be analyzed with neural networks trained on OCT data and known clinical outcomes to make more accurate predictions about the development and progression of retinal diseases, central nervous system disorders, and other conditions. The methods take 2D or 3D OCT data derived from different light source configurations and analyze it with neural networks that are trained on OCT images correlated with known clinical outcomes to identify intensity distributions or patterns indicative of different retina conditions. The methods have greater predictive power than traditional OCT analysis because the invention recognizes that subclinical physical changes affect how light interacts with the tissue matter of the retina, and these intensity changes in the image can be distinguishable by a neural network that has been trained on imaging data of retinas.

The utility model provides an ophthalmic surgical instrument for posterior detachment of a vitreous body, and relates to the technical field of medical instruments. The ophthalmic surgical instrument comprises an injection needle cylinder and a liquid supply pipe connected with the outlet end of the injection needle cylinder, and the end, away from the injection needle cylinder, of the liquid supply pipe is communicated with an injection needle with a silica gel soft head; a slidable piston is arranged in the injection needle cylinder, and the piston rod is detachably connected with the piston. The device is simple in structure, can be manufactured by existing medical common instruments, and can be reused, so that the cost of ophthalmologic operations is reduced; the silicone oil injection device can be connected with a silicone oil injection pipeline of the vitreous cutter for flow control and automatic injection, is stable in operation, saves manpower, and can reduce disturbance to retina tissues caused by unbalanced manual injection liquid flow.

A tissue transplantation resin tip includes a needle tube for eyeball insertion. The needle tube includes a main body portion that extends linearly. A front end portion is bent with respect to the main body portion and extends linearly, with tissue discharging from the front end. The needle tube length is 25 mm to 50 mm, the front end portion cross sectional shape is oval with a major axis D1 of 0.8 mm to 1.5 mm and a minor axis D2 of 0.5 mm to 1.0 mm. The front end of the front end portion is a bevel surface that extends in a direction substantially perpendicular to the main body portion and has a bevel angle of 50° to 85°. The front end portion hardness is 0.15 N to 0.30 N or less and the center of the needle tube hardness is 0.80 N to 1.50 N.

A composite hydrogel includes gelatin, cinnamic acid, polydopamine nanoparticles, and curcumin is provided. The gelatin is chemically cross-linked with the cinnamic acid to form cinnamic acid-modified gelatin (GelCA), and the curcumin is non-covalently linked to the polydopamine nanoparticles to form curcumin-loaded polydopamine nanoparticles (Cur@PDA). The GelCA undergoes a photo-crosslinking process and then binds with the Cur@PDA to form the composite hydrogel. A method for treating retinal tissue damage includes administering to a subject in need thereof an effective amount of the composite hydrogel.

The invention discloses a retinal tissue layering thickness evaluation method and a storage medium. The method comprises the following steps: acquiring a tissue scanning image of a retina tissue, and determining layering curves corresponding to a plurality of structural layers of the retina tissue in the tissue scanning image; determining a reference curve from the plurality of layered curves, and moving the reference curve along the arrangement direction of the plurality of structural layers to obtain a reference curve; determining a first position deviation value of the reference curve and the layering curve at a first reference point, determining a target statistical histogram according to the first position deviation value, and determining thickness characteristic data corresponding to the layering thickness of the retina tissue according to the target statistical histogram, the accuracy and the automation degree of retinal layering thickness measurement are remarkably improved, personal errors are reduced, and the accuracy and the detection efficiency of retinal tissue layering thickness detection are improved.

The invention discloses an application of a body fluid extracellular vesicle membrane protein in preparation of a product for diagnosing glaucoma disease, the outer vesicle membrane protein is selected from Tau protein and / or GFAP protein on outer vesicles, the invention firstly proposes that NDEVs in peripheral blood are captured through specific membrane proteins (GLAST and L1CAM); therefore, pathological changes of the central nervous system and the retina tissue can be reflected more accurately.

The present invention comprises a method for producing a retinal tissue having an epithelial structure, the method comprising the steps of: (1) inoculating pluripotent stem cells onto a region (A) on a culture substrate having, on the surface thereof, a region (A) having cell adhesiveness and a region (B) adjacent to at least a portion of the region (A) and having lower cell adhesiveness than the region (A); (2) culturing the pluripotent stem cells inoculated in the step (1) in a culture medium containing a ROCK inhibitor; (3) culturing the cells obtained in the step (2) in a culture medium containing a BMP signal transduction pathway acting substance to obtain a retinal tissue; (4) dispersing the retinal tissue obtained in step (3) to obtain a dispersed retinal cell population; and (5) subjecting the dispersed retinal cell population obtained in step (4) to suspension culture or adhesion culture in a culture medium containing a Wnt signal transduction pathway acting substance.

Methods and systems for measuring hemoglobin concentration (cHb). A retinal tissue having blood vessels is illuminated by light including two isosbestic wavelengths (λ1,λ2). The isosbestic wavelengths(λ1,λ2) correspond to isosbestic points where oxy- and deoxyhemoglobin (HbIO2,Hb) have the same molar extinction coefficient. Measurements are collected of a plurality of backscattered reflection intensities (I(χb,λ1), I(xt,λ1), I(χb,λ2), I(xx,X2)). Preferably, the measurements are spectrally resolved at least for the two isosbestic wavelengths (λ1,λ2) and position-resolved for at least a blood vessel location (xb) coinciding with a selected blood vessel and a tissue location (xt) coinciding with the retinal tissue without blood vessel. The hemoglobin concentration (cHb) can be calculated based on a combination of the measurements at the two isosbestic wavelengths (λt,λ2).

An object of the present invention is to provide a cell population suitable for transplant of retinal tissue and a method of production thereof. The present invention provides a cell population for transplant, comprising retinal cells with a modified bipolar cell-regulating gene and a method of production thereof.

Photovoltaic retinal prosthesis is provided with optically configurable confinement of electrical field. A video stream is projected onto a retinal implant. An array of photovoltaic pixels is configured to provide retinal stimulus responsive to the video stream. The photovoltaic pixels have a common return electrode. Each pixel has an active electrode that is coupled to retinal tissue via a capacitive interface or a faradaic interface. Each pixel includes photodiode(s) connected in series between the common return electrode and the corresponding active electrode. The projected video stream is configured based on a source video stream such that one or more pixels of the retinal implant that will be dark in a next frame of the projected video stream are optically preconditioned (pre-charged) by the projected video stream during the previous frame to become sufficiently conductive to act as transient local return electrodes during the next frame of the projected video stream.

The present disclosure provides methods and an apparatus for imaging and analysing images of presumed protein deposits in the retina, retinal tissue or retinal structures and discloses methods differentiating or classifying these deposits and other optical signals from retinal structures into 1) whether they contain or do not contain classes, of proteins or protein deposits called amyloids or other proteins and / or protein deposits related to neurodegenerative eye and brain disease(s); 2) which type(s) of amyloid or other proteins or protein deposits they contain, as well as 3) whether the form and / or properties of the deposit are associated with a class of diseases or with one or another specific condition(s) (or disease(s)); whether or not this is a disease or class of disease associated with the retina or more generally with the nervous system, including the brain or 4) classified as associated with one or another level of severity of condition(s), or disease(s).

The invention provides a medical image quality control method, system and device and a medium. The method comprises the following steps: acquiring a fundus image of a lesion retina; performing frequency domain conversion on the fundus image to obtain a high-frequency feature map and a low-frequency feature map; determining image sub-blocks of the high-frequency feature map, determining an identification scale according to edge distribution information of the focus part, extracting sub-region features according to the identification scale, and determining the texture complexity of the retina tissue of the fundus image in a high-frequency state based on the sub-region features; determining the feature loss of the color of the low-frequency feature map in frequency domain conversion, and determining the global chromaticity offset index of the fundus image in a low-frequency state based on the feature loss and the pixel color space of the fundus image; and determining a quality feedback coefficient of the fundus image according to the texture complexity and the chromaticity offset index, and adjusting the contrast of the fundus image based on the quality feedback coefficient. By adopting the scheme of the invention, the contrast ratio can be optimized and adjusted in the eye fundus image quality control process.