Method for establishing human bladder transitional cell carcinoma cell line and a mouse model of bladder carcinoma tissue recombination functional reconstruction

A method of establishment, technology of transitional cells, applied in cell dissociation methods, tumor/cancer cells, tissue culture, etc.

Inactive Publication Date: 2016-10-12
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0002] Stable human bladder transitional cell carcinoma cell lines and t...

Method used

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Experimental program
Comparison scheme
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Embodiment Construction

[0018] Experiment 1: Primary Cell Culture

[0019] Main experimental reagents and materials:

[0020] Tissue source: fresh clinical bladder cancer surgical specimens provided by the Department of Urology, Nantong First People's Hospital

[0021] Reagents: DMEM / F12 basal medium, RPMI1640 basal medium,

[0022] Vascular endothelial growth factor (BPE), epidermal growth factor (EGF), insulin-transferrin-selenium

[0023] (ITS), double antibody, fetal bovine serum, 0.25% trypsin, PBS

[0024] Materials: Surgical instruments (all sterilized by high temperature and high pressure), pipettes

[0025] Primary culture flasks, cell culture flasks, 15ml centrifuge tubes

[0026] CO 2 Incubator, inverted phase contrast microscope, centrifuge

[0027] experimental method:

[0028] The fresh surgical specimens of bladder cancer taken out from the clinic should be immediately placed in the primary cell culture medium and stored at 4°C. Divide the specimen into two parts, one part was ...

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Abstract

The invention discloses a method for establishing a human bladder transitional cell carcinoma cell line and a mouse model of bladder carcinoma tissue recombination functional reconstruction. The method includes the primary cell culture, immortalized cell line identification, tissue reorganization and planting, HE staining, and immunohistochemistry. The invention establishes a novel stable human bladder transitional cell carcinoma cell line, and uses the cell line to construct the mouse model of bladder carcinoma tissue recombination functional reconstruction. The occurrence and development of bladder carcinoma are simulated in mice. The method researches on the biological behavior of bladder carcinoma from in vitro molecular and cell biology and in vivo animal models, and provides an effective tool for the discovery of new biomarkers and therapeutic measures.

Description

technical field [0001] The invention relates to a bladder transitional cell carcinoma cell line and a method for establishing a bladder cancer tissue recombination functional reconstruction model. Background technique [0002] There is a lack of stable human bladder transitional cell carcinoma cell lines and their bladder cancer functional reconstruction models in the prior art. Translational medical research urgently needs to establish such a model. Contents of the invention [0003] The purpose of the present invention is to provide a bladder transitional cell carcinoma cell line and a method for establishing a bladder cancer tissue recombination functional reconstruction mouse model. Contents of the invention [0004] Technical solution of the present invention is: [0005] A method for establishing a human bladder transitional cell carcinoma cell line is characterized in that it comprises the following steps: [0006] (1) Primary cell culture [0007] Aseptically...

Claims

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Application Information

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IPC IPC(8): C12N5/095A01K67/027
CPCA01K67/0271A01K2227/105A01K2267/0331C12N5/0695C12N2509/00C12N2509/10
Inventor 江明王海燕曹靖晨鄂群张冲张永辉陈苗苗吕秀芳潘晓东钱麟
Owner NANTONG UNIVERSITY
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