The invention relates to a medicament complex which is prepared from total flavone extract of lotus leaf and hypericum japonicum thunb, and new usage for preparing anti-hepatitis B virus medicament. The total flavone of lotus leaf and hypericum japonicum thunb of the invention is total flavone extract which is separated and extracted from lotus leaf and hypericum japonicum thunb which grow in China. The complex of lotus leaf and hypericum japonicum thunb is separated and purified, tested by mass spectrum, 13C and 1HNMR, and proved to contain the following chemical components: quercetin, quercetin-3-O-beta-D-galactopyranose, isoquercetin, and kacmpferol and the like. The complex of total flavone extract is tested by in vitro cell model and in vivo animal model and proved to have strong activity of resisting hepatitis B virus and protecting liver. The complex of total flavone extract can be further prepared into anti-hepatitis B virus medicament and dosage forms can be capsules, tablets, granules, and injection and the like.