Method for researching influence of tumour-associated antigen p62/IMP2 on esophageal squamous cell carcinoma ESCC

Abstract

The invention discloses a method for researching influence of a tumour-associated antigen p62 / IMP2 on esophageal squamous cell carcinoma ESCC. The method comprises the following steps: step 1, detecting expression characteristics of the p62 / IMP2 in the ESCC; step 2, constructing a stable cell strain for stable over / low expression of the p62 / IMP2; and step 3, observing the effect of the p62 / IMP2 inthe esophageal squamous cell carcinoma. Due to the operation of the process steps, an esophageal squamous cell carcinoma cell model with stable over / low expression of the p62 / IMP2 is constructed in the invention; and cell biology experiments, molecular biology experiments and in-vivo animal models prove that the p62 / IMP2 plays a crucial role in proliferation, migration, invasion, apoptosis and chemotherapy drug resistance of the ESCC (Esophageal Squamous Cell Carcinoma). An essential basis is provided for deep exploration of an ESCC drug resistance mechanism and gene targeted therapy. According to the invention, the functions and molecular mechanisms of the p62 / IMP2 in ESCC growth, transfer, apoptosis and chemotherapy drug resistance are deeply explored by applying technologies, such as cytobiology function experiments, molecular biology experiments and nude mouse subcutaneous tumorigenesis models; and the correlation between clinical tissue sample analysis and clinical factors is combined.

Description

technical field [0001] The present invention relates to the technical field of tumor-associated antigen research, and more specifically relates to a research method for the effect of tumor-associated antigen p62 / IMP2 on esophageal squamous cell carcinoma. Background technique [0002] Esophageal cancer, also known as esophageal cancer, accounts for 22.34% of the total number of malignant tumor deaths in the country, and its mortality rate ranks second. In 2012, there were 456,000 new cases of esophageal cancer worldwide, and about 400,000 deaths, ranking the second among global malignant tumors. eight. In my country, especially in Lin County, Henan Province, esophageal cancer is a high-incidence area in the world, which seriously endangers people's health and causes a great burden to families and social economy. Therefore, research on esophageal cancer has attracted great attention. [0003] Esophageal cancer includes esophageal adenocarcinoma (esophagealadenocarcinoma, EA...

AI Technical Summary

Problems solved by technology

In 2016, AhmadBarghash's research team found that the high expression of p62 / IMP2 in esophageal cancer leads to shortened survival and is related to the metastasis of EAC, indicating that p62 / IMP2 may become a prognostic marker for Barret's esophagus and EAC

It can be seen that the research on the function of p62 / IMP2 in tumors has gradually become a hot spot, but we still know little about its molecular mechanism and other issues

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