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Method for evaluating in-vivo bioavailability of perchlorate in food matrix

A perchlorate and evaluation method technology, applied in biological testing, material inspection products, measuring devices, etc., can solve the problems of expanding the biological effective dose of pollutants, affecting the accuracy of risk assessment, etc., to achieve the effect of reducing differences

Inactive Publication Date: 2020-11-24
WUHAN POLYTECHNIC UNIVERSITY
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Problems solved by technology

[0002] Perchlorate (ClO 4 - ) is a new type of persistent pollutant, due to its ability to competitively inhibit the absorption of iodine by the thyroid gland, it may cause potential health risks for human beings for long-term exposure
In recent years, there have been reports on the exposure assessment of perchlorate in various foods and populations, but most of the risk assessments are based on the total concentration of perchlorate in the test medium, that is, assuming that all the high chlorine that can be detected salts can be absorbed by the human body, which expands the biologically effective dose of pollutants and affects the accuracy of risk assessment

Method used

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  • Method for evaluating in-vivo bioavailability of perchlorate in food matrix
  • Method for evaluating in-vivo bioavailability of perchlorate in food matrix
  • Method for evaluating in-vivo bioavailability of perchlorate in food matrix

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Embodiment Construction

[0039] The present invention will be further described below in conjunction with specific embodiments.

[0040] A method for evaluating the bioavailability in vivo of perchlorate in a food matrix, comprising the following steps:

[0041] (1) Pretreatment of food matrix: Wash the pre-purchased food samples (rice, pork, soybean, lettuce) three times with ultrapure water to remove surface impurities. The rice is washed three times with ultra-pure water in advance, and then put into the rice cooker to ripen. Pork, soybeans, and lettuce are washed and then cooked with ultra-pure water. Put the cured sample in a clean glass to cool to room temperature, put it in a -20°C refrigerator, freeze-dry it for 24 hours after solidification, and then grind and sieve it with a universal grinder. Milk powder does not need to be milled. ℃ airtight and dry storage.

[0042] (2) Preparation of perchlorate standard solution: Accurately draw 1000 mg / L sodium perchlorate original standard solution ...

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Abstract

The invention provides a method for evaluating the in-vivo bioavailability of a perchlorate in a food matrix. Different food matrixes are used as raw materials; the method comprises steps: adding a standard by adopting a perchlorate solution; preparing different feeds to feed ICR mice, carrying out diet exposure, determining the content of the perchlorate in blood, tissues, organs and urine excrement of the mice in different experimental groups and control groups by using a chromatography-mass spectrometry technology, and calculating the perchlorate in-vivo accumulation rate and the perchlorate bioavailability of the mice. The in-vivo animal model effectively simulates and evaluates the biological utilization rate of perchlorate exposed to human diet, so that the difference caused by the biological utilization rate evaluation of a traditional in-vitro cell model on the body action can be reduced, and technical guidance can be provided for the in-vivo biological utilization rate evaluation of other toxic and harmful pollutants in food.

Description

technical field [0001] The invention belongs to the field of bioavailability evaluation of toxic and harmful substances in food safety, and relates to a method for evaluating the in vivo bioavailability of perchlorate in a food matrix. Background technique [0002] Perchlorate (ClO 4 - ) is a new type of persistent pollutant, which may lead to potential health risks for long-term human exposure due to its ability to competitively inhibit the absorption of iodine by the thyroid gland. In recent years, there have been reports on the exposure assessment of perchlorate in various foods and populations, but most of the risk assessments are based on the total concentration of perchlorate in the test medium, that is, assuming that all the high chlorine that can be detected All acid salts can be absorbed by the human body, which expands the biologically effective dose of pollutants and affects the accuracy of risk assessment. [0003] Due to the complexity of the digestion proces...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/88G01N33/50
CPCG01N30/06G01N30/88G01N33/5014
Inventor 柳鑫张虎田一媚许琳宫智勇方敏吴永宁
Owner WUHAN POLYTECHNIC UNIVERSITY
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