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Follicular stem cell originated from human hair and its amplifying prepn process

A technology of hair follicle stem cells and stem cells, applied in the direction of artificially induced pluripotent cells, non-embryonic pluripotent stem cells, biochemical equipment and methods, etc., which can solve problems such as easy aging, poor proliferation activity, and insufficient source of seed cells

Inactive Publication Date: 2007-10-03
TIANJIN HOSPITAL
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Problems solved by technology

[0008] The present invention aims to solve the problems of insufficient source of seed cells, poor proliferative activity, easy aging and lack of appendages such as hair follicles in the development of tissue engineered skin, and provides an epidermal stem cell with good proliferative activity, anti-aging properties and endowed hair follicle stem cells. Phenotypic expression characteristics and follicle generating potential of seed cells

Method used

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  • Follicular stem cell originated from human hair and its amplifying prepn process
  • Follicular stem cell originated from human hair and its amplifying prepn process
  • Follicular stem cell originated from human hair and its amplifying prepn process

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Embodiment Construction

[0026] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0027] Using conventional aseptic techniques, take normal mammalian beard or human hair tissue, remove part of the hair shaft and adipose tissue at the root of the hair, and wash 3 times with Hank’s solution containing penicillin 10u / ml and streptomycin 10μg / ml. Enter the first step of enzyme digestion: add 5-10ml of 0.1-0.5u / ml Dispase II solution, digest at 4°C for 10-18h, take out the tissue and wash it with Hank's solution; enter the second step of enzyme digestion: put the hair follicle tissue in 0.01-0.5 % trypsin and 0.01-0.1% EDTA in a 1:1 mixture, digested in a shaker incubator at 37°C for 0.5-3 hours, added fetal calf serum to terminate the digestion, and filtered the tissue and cell mixture through a 100-mesh steel mesh, Collect the hair follicle cell mixture. Placenta blue staining was used to detect cell number and cell viability, and th...

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Abstract

The present invention relates to follicular stem cell originated from human hair and its amplifying preparation process, and belongs to the field of medical preparation and human cell culture technology. The present invention adopts improved two-step protease digesting method to amplify follicular stem cell originated from human hair and obtains follicular stem cell with active expression of beta-integrin and keratin 19 in an optimized follicular stem cell preparing environment. The optimized micro environment endows follicular stem cell with fast amplification, antisenile characteristic, the epidermic cell-like, fibroblst-like and hair bulb tissue-like structure or functional expression. The present invention makes it possible to provide sufficient seed cell sources for the fast repair and functional reconstruction of skin defect.

Description

technical field [0001] The invention relates to a medical preparation derived from skin hair follicles, or the cultivation of human cells, in particular to a hair follicle stem cell derived from human hair used as tissue engineering skin seed cells and a preparation method for its expansion. Background technique [0002] Due to the increasing number of skin defects and / or skin ulcers caused by trauma, burns, infection and metabolic diseases, accelerating the repair of large areas of skin defects and skin ulcers and reducing the disability rate is an important issue in the field of surgery. With the development of modern medical technology, the application of autologous and allogeneic skin transplantation, heterogeneous or heterogeneous dermal substitutes, and artificial synthetic substitutes has made great progress in skin repair technology. Quick fixes and functional rebuilds break new ground. [0003] The experimental strategy of tissue engineering skin is as follows: 1. ...

Claims

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Application Information

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IPC IPC(8): C12N5/08C12N5/074
Inventor 李秀兰张杨华云旗郭悦张文海白人骁
Owner TIANJIN HOSPITAL
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