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Application of polypyrimidine sequence binding protein in preparation of drugs for repairing spinal cord injury

A technology combining proteins and polypyrimidines, applied in the field of biomedicine, can solve the problems of difficult tissue repair and functional reconstruction of SCI, limited neuron regeneration ability, and insufficient replenishment of neurons, and achieve good spinal cord injury repair and functional reconstruction. effect of effect

Pending Publication Date: 2021-07-27
NANTONG UNIVERSITY
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Problems solved by technology

Currently recognized SCI treatment has three major difficulties: First, rapidly activated reactive glial cells, especially astrocytes, will proliferate in large numbers to form dense glial scar tissue, allowing relatively slow-growing axons to extend through the injury When in the process of living, it encounters an insurmountable mechanical barrier and a chemical barrier formed by the secreted inhibitory factors; second, some damaged neurons will be lost due to necrosis or apoptosis, and only a small amount of residual neural stem cells cannot fully replenish the lost neurons. neurons, and the damaged neurons that have not been lost have limited regenerative ability; third, there will be a variety of inhibitory regeneration factors and inflammatory factors at the injury site, forming a chemical microenvironment that is unfavorable for axon regeneration
These unfavorable factors coordinate with each other, and ultimately lead to difficult tissue repair and functional reconstruction in SCI

Method used

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  • Application of polypyrimidine sequence binding protein in preparation of drugs for repairing spinal cord injury
  • Application of polypyrimidine sequence binding protein in preparation of drugs for repairing spinal cord injury
  • Application of polypyrimidine sequence binding protein in preparation of drugs for repairing spinal cord injury

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Embodiment 1

[0019] 1. Obtain high-purity mouse spinal cord astrocytes from primary culture

[0020] Spinal cord tissue was obtained from neonatal mice. Place the spinal cord in a petri dish filled with ice D-Hanks solution and wash it twice, use microsurgical tweezers to carefully peel off the meninges and blood vessels on the surface, rinse it 2-3 times with D-Hanks solution, and transfer it to another dish. In a petri dish, cut up the spinal cord until it was chyle-shaped, transfer the chopped spinal cord pieces in the petri dish into a 15 mL centrifuge tube, then add an equal amount of 0.25% trypsin, and digest in a water bath at 37°C for 15 min, every 5 min Pipette and mix until there is no obvious tissue block, then add twice the amount of 10% FBS complete medium to stop the digestion of the enzyme; centrifuge at 1000 rpm for 5 min, then discard the supernatant, collect the cell pellet, and then use 5 mL basic The medium was used to resuspend the cells, and the centrifugation was re...

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Abstract

The invention discloses an application of a polypyrimidine sequence binding protein silencing agent combined with retinoic acid and purinine amine in preparation of a medicine for repairing spinal cord injury, and belongs to the technical field of biological medicine. The polypyrimidine sequence binding protein (PTB) is silenced in vitro through a virus, and meanwhile, micromolecule retinoic acid (RA) and purinol amine (PMA) which are related to motor neuron differentiation are jointly added, so that mouse spinal cord reactive astrocytes are successfully reprogrammed into motor neuron; and help is provided for further in-vivo research on the effect of a PTB combined micromolecule reprogramming strategy in spinal cord injury post-repair, so that better spinal cord injury repair and function reconstruction effects are realized.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the application of a polypyrimidine sequence-binding protein silencing agent in combination with retinoic acid and purmorphamine in the preparation of spinal cord injury repairing medicine. Background technique [0002] Spinal cord injury (SCI) is a central nervous system injury disease with high disability rate and serious consequences, which seriously impairs the motor function of patients and often leads to paralysis below the injury site. Currently recognized SCI treatment has three major difficulties: First, rapidly activated reactive glial cells, especially astrocytes, will proliferate in large numbers to form dense glial scar tissue, allowing relatively slow-growing axons to extend through the injury When in the process of living, it encounters an insurmountable mechanical barrier and a chemical barrier formed by the secreted inhibitory factors; second, some...

Claims

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Application Information

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IPC IPC(8): A61K31/7088A61P25/00A61K31/203A61K31/5377
CPCA61K31/7088A61K31/203A61K31/5377A61P25/00A61K2300/00
Inventor 杨日云潘静莹包璟崟夏盼慧
Owner NANTONG UNIVERSITY
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