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Composite marssonia extract as well as preparation method and application of composite marssonia extract

A technology of compounding Marseille bacteria and extracts, applied in the field of biomedicine, can solve the problems of insignificant effect and high cost, and achieve the effect of eliminating melanin deposition, cost-effective, and delaying skin aging

Active Publication Date: 2022-01-07
北京达尔文细胞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing cell repair products include active peptides, vitamins, probiotics, etc., which have defects such as insignificant effect and high cost

Method used

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  • Composite marssonia extract as well as preparation method and application of composite marssonia extract
  • Composite marssonia extract as well as preparation method and application of composite marssonia extract
  • Composite marssonia extract as well as preparation method and application of composite marssonia extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Example 1 Preparation of compound Marseille extract of the present invention

[0089] The preparation of compound Marseille extract comprises the following steps:

[0090] (1) Take out the three starting strains of Marseilles M.violaceinigra B2T, M.putida 6NM-7T, and Massilia sp.NR4-1 frozen at -80°C, respectively, and place them in a constant temperature water bath at 37°C to melt, Spread the plate and place it in a 28°C incubator for one week until colonies grow;

[0091](2) Pick a single colony, inoculate it into 50ml of basal medium, culture it on a shaker at 37°C and 200rpm for 48 hours, then inoculate it into the acclimatization medium according to the inoculum size of 15%, and cultivate it at 25°C and 250rpm 120h, get OD 650 Three kinds of bacterial liquids of 1.0-2.0;

[0092] (3) After mixing the three bacterial solutions at a ratio of 1:1:1, inoculate 10% of the inoculum into the acclimatization medium, and culture at 250prm and 10-31°C for 72 hours, in ...

Embodiment 2

[0094] Example 2 Preparation of compound Marseille extract of the present invention

[0095] The preparation of compound Marseille extract comprises the following steps:

[0096] (1) Take out the three starting strains of Marseilles M.violaceinigra B2T, M.putida 6NM-7T, and Massilia sp.NR4-1 frozen at -80°C, respectively, and place them in a constant temperature water bath at 37°C to melt, Spread the plate and place it in a 28°C incubator for one week until colonies grow;

[0097] (2) Pick a single colony, inoculate it into 50ml of basal medium, culture it on a shaker at 37°C and 200rpm for 48 hours, then inoculate it into the acclimatization medium according to the inoculum size of 15%, and cultivate it at 25°C and 250rpm 120h, get OD 650 Three kinds of bacterial liquids of 1.0-2.0;

[0098] (3) After mixing the three bacterial solutions at a ratio of 1:1:1, they were inoculated into the acclimatization medium according to the inoculum size of 5%, and cultured at 250prm...

Embodiment 3

[0100] Example 3 Preparation of compound Marseille extract of the present invention

[0101] The preparation of compound Marseille extract comprises the following steps:

[0102] (1) Take out the three starting strains of Marseilles M.violaceinigra B2T, M.putida 6NM-7T, and Massilia sp.NR4-1 frozen at -80°C, respectively, and place them in a constant temperature water bath at 37°C to melt, Spread the plate and place it in a 28°C incubator for one week until colonies grow;

[0103] (2) Pick a single colony, inoculate it into 50ml of basal medium, culture it on a shaker at 37°C and 200rpm for 48 hours, then inoculate it into the acclimatization medium according to the inoculum size of 15%, and cultivate it at 25°C and 250rpm 120h, get OD 650 Three kinds of bacterial liquids of 1.0-2.0;

[0104] (3) After mixing the three bacterial solutions at a ratio of 1:1:1, they were inoculated into the acclimation medium at an inoculum size of 15%, and cultured at 250prm and 10-35°C f...

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Abstract

The invention relates to a compound marssonia extract as well as a preparation method and application of the compound marssonia extract. The compound marssonia extract disclosed by the invention contains a plurality of growth factors and proteins with the functions of regulating and controlling cell proliferation and differentiation, repairing cells and nourishing cells, maintaining tissue and cell growth and development and the like, promotes cell repair and organ injury repair, delays skin aging, reduces or even eliminates melanin deposition, and is used for joint repair, hair follicle repair, organ injury repair, skin cell repair, oxidative injury cell repair and the like, and is safe and effective.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a compound Marseille extract, a preparation method thereof and an application thereof. Background technique [0002] Hair is one of the important indicators of human health and plays an important role in appearance. Affected by factors such as busy life, work pressure, and environmental pollution, modern people have problems such as easy hair breakage and premature aging, which seriously affect their personal image. There are more and more men and women with hair loss and a younger trend. According to the results of "China Hair Loss Population Survey", there are about 250 million hair loss people in my country, and the incidence rate of hair loss is as high as 12%. About 60% of the population has hair loss symptoms around the age of 25, and the hair loss population is showing a trend of younger age. The results of the study show that the number and activity of hair follicle stem cel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61K9/19A61K9/08C12N1/20C12N1/36A61P17/00A61P17/10A61P17/18A61P19/02A61P39/06A61P43/00C12R1/01
CPCA61K35/74A61K9/19A61K9/0014C12N1/20C12N1/36A61P17/00A61P19/02A61P39/06A61P17/10A61P17/18A61K2300/00Y02A50/30
Inventor 王宇高文勇陈琳李建军陈育新舒磊晶
Owner 北京达尔文细胞生物科技有限公司
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